FastQCFastQC Report
Thu 26 May 2016
SRR1364399_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364399_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1311656
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20520.15644345773586976No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15800.058.031261
GTATCAA24100.051.880081
ATCAACG28450.042.469743
TCAACGC29750.040.6139224
CAACGCA30500.039.615225
AACGCAG30900.039.287536
TATCAAC31500.038.7355042
GTACATG72650.036.0591471
TACATGG72250.035.0122
ACATGGG74350.032.422013
ACGCAGA38300.031.2258427
CGCAGAG39450.030.3121138
CATGGGA49150.029.063884
ATGGGAT19250.027.5185245
CATGGGG33050.025.7531554
GTAAGGT6050.025.5790164
GCAGAGT47950.024.69069
GTGGTAT9550.024.3141781
TAAGGTG7850.024.2630925
CAGTGCG3052.764864E-1023.407159
Produced by FastQC (version 0.11.5)