FastQCFastQC Report
Thu 26 May 2016
SRR1364399_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364399_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1311656
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51620.3935483083979336No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46920.3577157425422519No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28340.2160627481595784No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30300.082.9404141
GTATCAA53300.054.8593561
ATCAACG69550.041.77693
TATCAAC69850.041.5122342
TCAACGC69850.041.5122344
CAACGCA71900.040.3286445
AACGCAG72800.039.890566
GTACATG68700.037.274181
TACATGG70950.035.749562
TGGTATC10800.035.2833442
GTGGTAT11900.035.030591
ACGCAGA85100.034.1883547
ACATGGG72550.034.1404653
CGCAGAG86400.033.7428058
GCAGAGT96450.029.9800919
CATGGGA47900.029.9567784
GAGTACT60000.029.55043612-13
CAGAGTA93400.028.4110210-11
CATGGGG31600.027.5092854
GTACTTT66050.026.75361814-15
Produced by FastQC (version 0.11.5)