##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364399_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1311656 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.3047613093677 33.0 31.0 34.0 30.0 34.0 2 32.29921336082022 33.0 31.0 34.0 30.0 34.0 3 32.40538830303067 34.0 31.0 34.0 30.0 34.0 4 36.025810120946346 37.0 35.0 37.0 35.0 37.0 5 35.9664447080637 37.0 35.0 37.0 35.0 37.0 6 36.03453649432473 37.0 35.0 37.0 35.0 37.0 7 35.98944997773807 37.0 35.0 37.0 35.0 37.0 8 35.99782946138317 37.0 35.0 37.0 35.0 37.0 9 37.73133428276926 39.0 38.0 39.0 35.0 39.0 10-11 37.695868428917336 39.0 37.5 39.0 35.0 39.0 12-13 37.6617295235946 39.0 37.0 39.0 35.0 39.0 14-15 39.04921602920278 41.0 38.0 41.0 35.5 41.0 16-17 39.06466596424673 41.0 38.0 41.0 35.5 41.0 18-19 39.13212153186507 41.0 38.5 41.0 36.0 41.0 20-21 39.14455619461201 41.0 39.0 41.0 35.5 41.0 22-23 39.10057095762913 41.0 39.0 41.0 35.0 41.0 24-25 39.05991433729575 41.0 39.0 41.0 35.0 41.0 26-27 38.96324493617229 41.0 38.5 41.0 35.0 41.0 28-29 38.8584838555231 40.5 38.0 41.0 35.0 41.0 30-31 38.71540060808627 40.0 38.0 41.0 35.0 41.0 32-33 38.55459472605622 40.0 38.0 41.0 34.0 41.0 34-35 38.43791093091481 40.0 38.0 41.0 34.0 41.0 36-37 38.30468506986588 40.0 38.0 41.0 34.0 41.0 38-39 38.19743210109968 40.0 38.0 41.0 33.5 41.0 40-41 38.058299965844704 40.0 38.0 41.0 33.0 41.0 42-43 37.880743884067165 40.0 37.0 41.0 33.0 41.0 44-45 37.67108792244308 40.0 37.0 41.0 33.0 41.0 46-47 37.35404404813457 40.0 37.0 41.0 32.0 41.0 48-49 37.07190071177199 40.0 37.0 41.0 31.0 41.0 50-51 36.887030212189785 40.0 36.0 41.0 31.0 41.0 52-53 36.72306420280927 40.0 36.0 41.0 31.0 41.0 54-55 36.53753728111639 39.5 35.5 41.0 31.0 41.0 56-57 36.3215827930494 39.0 35.0 41.0 30.0 41.0 58-59 36.08384706050977 39.0 35.0 41.0 30.0 41.0 60-61 35.81946485968882 38.5 35.0 41.0 29.0 41.0 62-63 35.572283815268634 38.0 35.0 40.5 29.0 41.0 64-65 35.26802835499552 37.0 35.0 40.0 29.0 41.0 66-67 34.93713214440372 37.0 34.5 40.0 29.0 41.0 68-69 34.59596113615155 36.0 34.0 39.0 28.0 41.0 70-71 34.33402698573407 36.0 34.0 39.0 28.5 41.0 72-73 34.13910621382436 35.5 34.0 39.0 29.0 40.5 74-75 33.8167168068457 35.0 34.0 37.5 28.5 40.0 76-77 33.4961262709125 35.0 34.0 37.0 28.0 39.0 78-79 33.19822384832609 35.0 34.0 36.5 28.0 39.0 80-81 32.9221689985789 35.0 34.0 36.0 27.0 38.5 82-83 32.659173213098555 35.0 34.0 36.0 27.0 37.0 84-85 32.45197902498826 35.0 33.5 35.0 27.0 37.0 86-87 32.27326257799301 35.0 33.0 35.0 26.0 36.5 88-89 32.12019614899029 35.0 33.0 35.0 26.0 36.0 90-91 31.986819714925254 35.0 33.0 35.0 26.0 36.0 92-93 31.86286381490269 35.0 33.0 35.0 25.5 36.0 94-95 31.776562604829316 35.0 33.0 35.0 25.0 35.0 96-97 31.69672650451033 35.0 33.0 35.0 25.0 35.0 98-99 31.63040957385168 35.0 33.0 35.0 25.0 35.0 100-101 31.560518535347683 35.0 33.0 35.0 25.0 35.0 102-103 30.572147346560378 34.0 30.5 35.0 23.5 35.0 104-105 31.295546240782645 35.0 32.5 35.0 24.5 35.0 106-107 31.59901872137207 35.0 33.0 35.0 25.0 35.0 108-109 31.6146440072702 35.0 33.0 35.0 25.0 35.0 110-111 31.578278908494298 35.0 33.0 35.0 25.0 35.0 112-113 31.51266909921504 35.0 33.0 35.0 25.0 35.0 114-115 31.428777438596704 35.0 33.0 35.0 25.0 35.0 116-117 31.37038865373238 35.0 33.0 35.0 24.0 35.0 118-119 31.257795489061156 35.0 33.0 35.0 24.0 35.0 120-121 31.12247952206981 35.0 33.0 35.0 23.0 35.0 122-123 30.97087079234189 35.0 33.0 35.0 19.5 35.0 124-125 30.276213809108487 34.5 32.0 35.0 10.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.050163900371281045 1101 2 0.10682820962816919 1101 3 -0.0016086706103450865 1101 4 0.08733901611135764 1101 5 0.1724727687978529 1101 6 0.1435457714912829 1101 7 0.23412475534938437 1101 8 0.1342437434712025 1101 9 0.05491075164545833 1101 10-11 0.13033310064601977 1101 12-13 0.13635441205246934 1101 14-15 0.09794587444611835 1101 16-17 0.23993957169850688 1101 18-19 0.2017689438110324 1101 20-21 0.0968217503360762 1101 22-23 0.08336808778992832 1101 24-25 0.11121554913004417 1101 26-27 -0.024904488805276515 1101 28-29 -0.07809754118427037 1101 30-31 0.04584285818145162 1101 32-33 -0.15094450307708485 1101 34-35 -0.1090673824596422 1101 36-37 -0.08295647104230852 1101 38-39 -0.0615270412575839 1101 40-41 -0.05442450194157544 1101 42-43 -0.10322879438431443 1101 44-45 -0.0811225715045083 1101 46-47 -0.1439756255850284 1101 48-49 -0.33387359498880187 1101 50-51 -0.28715770571466237 1101 52-53 -0.2520298650828465 1101 54-55 -0.20496364861998728 1101 56-57 -0.0637375988822626 1101 58-59 -0.30800334895415205 1101 60-61 -0.1479002689316431 1101 62-63 -0.20085206689926594 1101 64-65 -0.20190666221273545 1101 66-67 -0.21292884713989224 1101 68-69 -0.24593469824117165 1101 70-71 -0.20769061714516823 1101 72-73 -0.17057838472879894 1101 74-75 -0.22110291947489458 1101 76-77 -0.35246406225115834 1101 78-79 -0.4866352835055068 1101 80-81 -0.4662948368645985 1101 82-83 -0.49648791252428737 1101 84-85 -0.6629037491199199 1101 86-87 -0.5324624888902889 1101 88-89 -0.5165041721329757 1101 90-91 -0.8828609243288561 1101 92-93 -0.8012765229178918 1101 94-95 -0.6678767618058146 1101 96-97 -0.5324178345831179 1101 98-99 -0.4210999671175202 1101 100-101 -0.648283564167091 1101 102-103 -0.35654169595753515 1101 104-105 -0.44698798544626683 1101 106-107 -0.4275852306241248 1101 108-109 -0.38708117520820196 1101 110-111 -0.44624689755760727 1101 112-113 -0.4026832500718527 1101 114-115 -0.48218026029724825 1101 116-117 -0.4619615554652121 1101 118-119 -0.5285827672275261 1101 120-121 -0.401255351699465 1101 122-123 -0.3661382432350031 1101 124-125 -0.495448971563043 1102 1 0.15418005358829134 1102 2 0.1670108704787694 1102 3 0.08468239400252742 1102 4 0.06382057963371324 1102 5 0.06115103351977069 1102 6 0.04324843643130549 1102 7 0.19424011112909767 1102 8 0.1892635090110062 1102 9 0.18279038632375944 1102 10-11 0.2605833221563856 1102 12-13 0.19341675869914354 1102 14-15 0.25405743047119955 1102 16-17 0.302969918617805 1102 18-19 0.284505193129462 1102 20-21 0.2885240574516672 1102 22-23 0.2659117934878594 1102 24-25 0.31698589400463817 1102 26-27 0.2988478114946105 1102 28-29 0.3231407869236804 1102 30-31 0.315930013988428 1102 32-33 0.39416267385857395 1102 34-35 0.3473483243287916 1102 36-37 0.4179785582652116 1102 38-39 0.5171964626955301 1102 40-41 0.42890258366365686 1102 42-43 0.4231735365862406 1102 44-45 0.3618449005511124 1102 46-47 0.452532604376799 1102 48-49 0.4437172242587408 1102 50-51 0.41168618059940343 1102 52-53 0.41890371588770847 1102 54-55 0.4340128031248298 1102 56-57 0.5167984561886243 1102 58-59 0.46174089177649336 1102 60-61 0.4237988007248461 1102 62-63 0.43918720859146987 1102 64-65 0.47215575259096454 1102 66-67 0.40762390640426815 1102 68-69 0.39096722378718596 1102 70-71 0.39139480065744436 1102 72-73 0.39516759400620316 1102 74-75 0.391062259096536 1102 76-77 0.39193641144022706 1102 78-79 0.4123283035991818 1102 80-81 0.4683958153982175 1102 82-83 0.4134574766523684 1102 84-85 0.3836432451548859 1102 86-87 0.4187562440704511 1102 88-89 0.3960333142356163 1102 90-91 0.445770993610072 1102 92-93 0.42472155164590575 1102 94-95 0.4471723112585302 1102 96-97 0.4786427740591961 1102 98-99 0.5206236390711325 1102 100-101 0.43194365434435866 1102 102-103 0.5517402356128009 1102 104-105 0.4388340735777234 1102 106-107 0.4824943427128403 1102 108-109 0.48903489622036744 1102 110-111 0.39189505650455203 1102 112-113 0.3546883591157126 1102 114-115 0.36776776996447325 1102 116-117 0.25537631599062394 1102 118-119 0.17201616270703823 1102 120-121 0.14438430206280017 1102 122-123 0.0646269898620524 1102 124-125 -0.024601012866206418 1103 1 0.056893730461212044 1103 2 0.15158163731503294 1103 3 0.11001402122955284 1103 4 0.08174426176225325 1103 5 0.026741367684770978 1103 6 0.04983090067453588 1103 7 0.053382803963565095 1103 8 0.14704919459902044 1103 9 0.20127620572182536 1103 10-11 0.2097512577114813 1103 12-13 0.22154584882454742 1103 14-15 0.36277528482869315 1103 16-17 0.2771681555662653 1103 18-19 0.279386874206871 1103 20-21 0.2117779713113066 1103 22-23 0.3054752283435178 1103 24-25 0.3140128687157784 1103 26-27 0.38653109694217846 1103 28-29 0.34209730484624146 1103 30-31 0.348715446361453 1103 32-33 0.374573681170304 1103 34-35 0.41237955980410135 1103 36-37 0.2854568070978942 1103 38-39 0.3391354029964404 1103 40-41 0.22950337842109292 1103 42-43 0.10318913462939605 1103 44-45 0.1780516162627066 1103 46-47 0.1880484258118429 1103 48-49 0.054988611077831706 1103 50-51 -0.13700002751180307 1103 52-53 -0.05377860734290607 1103 54-55 -0.05302877927751126 1103 56-57 -0.04504311450956777 1103 58-59 -0.07373143295090046 1103 60-61 -0.07144160158312474 1103 62-63 -0.02487933405613063 1103 64-65 -0.03646059069203034 1103 66-67 -0.10130557941175056 1103 68-69 -0.21786936026387593 1103 70-71 0.06110032850940428 1103 72-73 0.08238743975888951 1103 74-75 -0.0076919794409917586 1103 76-77 0.026383159203213324 1103 78-79 0.08452406016617431 1103 80-81 0.09871459618494072 1103 82-83 0.0990280487944446 1103 84-85 0.0032912650699614687 1103 86-87 0.0628018092631848 1103 88-89 0.026933926790803753 1103 90-91 0.12595765244396162 1103 92-93 0.05823928175923854 1103 94-95 0.02425798683048086 1103 96-97 0.008427327494871406 1103 98-99 0.05303487596444967 1103 100-101 0.028899109889373165 1103 102-103 0.14706048504644542 1103 104-105 0.09786996595797604 1103 106-107 0.0481451108545734 1103 108-109 -0.012844803027750373 1103 110-111 0.05539006479312292 1103 112-113 0.15443075506967574 1103 114-115 0.06078848970275175 1103 116-117 -0.04606571522121783 1103 118-119 -0.0433479660702254 1103 120-121 -0.001903341158406846 1103 122-123 -0.10817198107224257 1103 124-125 -0.32934581154903597 1104 1 0.18040577206785002 1104 2 0.09625257499703821 1104 3 0.18565084187756042 1104 4 0.14282591172291603 1104 5 0.21226487359573554 1104 6 0.19876978837135795 1104 7 0.257737604768721 1104 8 0.2246938011649462 1104 9 0.20175643950371835 1104 10-11 0.2938560304178566 1104 12-13 0.31464478720135247 1104 14-15 0.27135205581848965 1104 16-17 0.2378477374563417 1104 18-19 0.33667007930756654 1104 20-21 0.31594527658672433 1104 22-23 0.24733884194946398 1104 24-25 0.3501249164530549 1104 26-27 0.22696575960348753 1104 28-29 0.2881476374336813 1104 30-31 0.37400435296109436 1104 32-33 0.32756952255010674 1104 34-35 0.30279685230734543 1104 36-37 0.44148936081766266 1104 38-39 0.35743026653852183 1104 40-41 0.28650304123567594 1104 42-43 0.26777393676285755 1104 44-45 0.2615484334366869 1104 46-47 0.29362833364783114 1104 48-49 0.22008430021330838 1104 50-51 0.22113431533423977 1104 52-53 0.3021589213944793 1104 54-55 0.3462549571456677 1104 56-57 0.29756537234531066 1104 58-59 0.2752963490842646 1104 60-61 0.2644487211952864 1104 62-63 0.35028031813200045 1104 64-65 0.35234740775487694 1104 66-67 0.28135644840094187 1104 68-69 0.37567404980468666 1104 70-71 0.38925071320731774 1104 72-73 0.44628458798764115 1104 74-75 0.5121074463220197 1104 76-77 0.5240560689090472 1104 78-79 0.38016415049099805 1104 80-81 0.42881146036051376 1104 82-83 0.3645590136556365 1104 84-85 0.3349080180765327 1104 86-87 0.3551645594700972 1104 88-89 0.41167526470168525 1104 90-91 0.3821368307333053 1104 92-93 0.4626012349824258 1104 94-95 0.3985082601550296 1104 96-97 0.28764240070497493 1104 98-99 0.3913876740925559 1104 100-101 0.41216909323445705 1104 102-103 0.39522895514422984 1104 104-105 0.3229182447796646 1104 106-107 0.3619370163609119 1104 108-109 0.35297072589356304 1104 110-111 0.3770036503002814 1104 112-113 0.32162108091340613 1104 114-115 0.2562027679726846 1104 116-117 0.23156560828265071 1104 118-119 0.223087268784532 1104 120-121 0.16564205115172115 1104 122-123 0.14322274044325312 1104 124-125 0.09929860087982334 1105 1 0.08615610110533112 1105 2 0.07651341572642423 1105 3 0.08929821462398735 1105 4 0.07036634883298376 1105 5 0.03756708345058968 1105 6 0.14113055762906157 1105 7 0.19111189185418453 1105 8 0.15664457633399564 1105 9 0.15329266864150526 1105 10-11 0.19184385551615435 1105 12-13 0.1461222427272304 1105 14-15 0.18343893098197128 1105 16-17 0.22696027857315215 1105 18-19 0.21595915237963936 1105 20-21 0.19215432197638904 1105 22-23 0.20497024582211765 1105 24-25 0.19294682736268243 1105 26-27 0.19223647145728506 1105 28-29 0.20994703991397756 1105 30-31 0.301569892695845 1105 32-33 0.27869226640343925 1105 34-35 0.2036961956741763 1105 36-37 0.1768995866228522 1105 38-39 0.23265593269781704 1105 40-41 0.3060224559917941 1105 42-43 0.3203181070837857 1105 44-45 0.359016518345868 1105 46-47 0.39456302665946197 1105 48-49 0.3451279401608289 1105 50-51 0.36729466935591404 1105 52-53 0.34096358847446595 1105 54-55 0.27255390855110306 1105 56-57 0.31427535283992825 1105 58-59 0.17884421245184257 1105 60-61 0.1439910089480847 1105 62-63 0.25416429920745287 1105 64-65 0.18986198015033295 1105 66-67 0.24458985698207414 1105 68-69 0.1257612009683129 1105 70-71 0.3583108915363695 1105 72-73 0.28592129765403484 1105 74-75 0.3203042127950084 1105 76-77 0.3073028861536926 1105 78-79 0.3863321872031307 1105 80-81 0.3677049778587218 1105 82-83 0.38663889612901414 1105 84-85 0.3742596649117047 1105 86-87 0.29001488088667315 1105 88-89 0.4136641649647075 1105 90-91 0.4233683423283985 1105 92-93 0.4613630962944164 1105 94-95 0.33861674468768754 1105 96-97 0.34920690955861033 1105 98-99 0.24452084210043523 1105 100-101 0.28963661687469155 1105 102-103 0.36263841062067215 1105 104-105 0.34399234875757045 1105 106-107 0.3769147870212777 1105 108-109 0.4498268216862158 1105 110-111 0.37153900200478773 1105 112-113 0.35916894325112736 1105 114-115 0.39403947784504467 1105 116-117 0.42063368557092673 1105 118-119 0.29834455137778093 1105 120-121 0.3476991584642839 1105 122-123 0.34249432664077517 1105 124-125 0.19966391487117718 1106 1 0.11987311851800087 1106 2 0.06606423136419437 1106 3 0.07195428967136763 1106 4 0.03720126675486313 1106 5 0.08126493149626413 1106 6 -0.05363634365957637 1106 7 -0.0135107149423348 1106 8 -0.0413760727736161 1106 9 0.13841613202583858 1106 10-11 0.05412659279888743 1106 12-13 0.06630082051450614 1106 14-15 0.03897702836790273 1106 16-17 0.06837654661985937 1106 18-19 0.10144345090741069 1106 20-21 0.07625235281355458 1106 22-23 0.041680473836692045 1106 24-25 0.03070479283804417 1106 26-27 0.05397098264832323 1106 28-29 -0.07083497728108767 1106 30-31 0.064015886120103 1106 32-33 0.04263803034920244 1106 34-35 0.1667879930051015 1106 36-37 0.21809765227744293 1106 38-39 0.20975679838563366 1106 40-41 0.10108703638246652 1106 42-43 0.10090279255977208 1106 44-45 0.17813529126898686 1106 46-47 0.24060175313297094 1106 48-49 0.16790105912959774 1106 50-51 0.15223425320854034 1106 52-53 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-0.7887727318026521 2216 42-43 -0.7667714669592343 2216 44-45 -0.6357705247083487 2216 46-47 -0.7174025878848909 2216 48-49 -0.7019515671556462 2216 50-51 -0.6236006746106852 2216 52-53 -0.7466347086284202 2216 54-55 -0.5662050140333577 2216 56-57 -0.5826017856930576 2216 58-59 -0.5671078732049679 2216 60-61 -0.6387311313000268 2216 62-63 -0.5248365781836029 2216 64-65 -0.43369943894783347 2216 66-67 -0.40094964802457866 2216 68-69 -0.6144305279402573 2216 70-71 -0.6753816147855005 2216 72-73 -0.7925319429183233 2216 74-75 -0.6764724739419066 2216 76-77 -0.67618257570048 2216 78-79 -0.5457197788251094 2216 80-81 -0.4976734552139206 2216 82-83 -0.434085136801599 2216 84-85 -0.3328520292115016 2216 86-87 -0.4336097353624666 2216 88-89 -0.41448073901354476 2216 90-91 -0.4448593492712263 2216 92-93 -0.4489234520422478 2216 94-95 -0.5129175692321439 2216 96-97 -0.5900272952475625 2216 98-99 -0.6181991305263423 2216 100-101 -0.5103223146732887 2216 102-103 -0.6593647774633595 2216 104-105 -0.6791290173523805 2216 106-107 -0.599183524733526 2216 108-109 -0.44519038569352887 2216 110-111 -0.3464885832391822 2216 112-113 -0.3003761751621923 2216 114-115 -0.4481517632409897 2216 116-117 -0.4931805264405398 2216 118-119 -0.41519297648240183 2216 120-121 -0.34908035092069767 2216 122-123 -0.34428869920347793 2216 124-125 -0.24609157657986458 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 198.0 3 16.0 4 40.0 5 127.0 6 306.0 7 582.0 8 1045.0 9 1736.0 10 2920.0 11 3982.0 12 5044.0 13 7421.0 14 11528.0 15 9948.0 16 4692.0 17 3969.0 18 3952.0 19 3736.0 20 3405.0 21 3542.0 22 3579.0 23 4062.0 24 5170.0 25 6792.0 26 8793.0 27 11211.0 28 14434.0 29 18631.0 30 23644.0 31 29303.0 32 37768.0 33 48570.0 34 67992.0 35 105226.0 36 213949.0 37 438439.0 38 204769.0 39 1135.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.69021487139441 18.350090292351993 13.1781840255747 23.781510810678903 2 16.308239355440758 20.52557987765085 38.2635386107333 24.902642156175094 3 18.78152503400282 25.13486767872064 30.00436089950414 26.079246387772404 4 12.776520673103315 16.50447983312698 36.355873796178265 34.36312569759144 5 12.937279782015185 38.529899299497984 34.15860805832864 14.374212860158195 6 32.38804900329566 36.620630125161846 16.70560658582821 14.285714285714285 7 28.33326725909842 33.36576053477437 20.600447068438676 17.70052513768854 8 27.326448398055586 34.60747330092646 19.160664076556657 18.905414224461293 9 27.461925992790793 14.719026939990362 19.28706917057521 38.53197789664363 10-11 25.40086730057271 26.21255115670572 27.038949236690108 21.347632306031457 12-13 26.31414029288167 23.873866318607927 27.65915758400068 22.152835804509717 14-15 23.007442500167727 24.807037820892063 25.933972017053254 26.25154766188696 16-17 22.356128436114346 28.327511176710967 26.596874485383363 22.719485901791323 18-19 22.32963520923169 26.071660557341254 29.364787718731133 22.233916514695924 20-21 23.04723356500047 25.279609225617772 29.744398666719523 21.92875854266223 22-23 22.980415596772325 25.247740261166037 29.628385796275854 22.143458345785785 24-25 22.507273248473687 25.560131619875943 29.547190726836913 22.385404404813457 26-27 22.344811339275196 25.91175947001247 29.42240462134245 22.321024569369882 28-29 22.35094011073042 25.89714970563883 29.511898717192185 22.24001146643856 30-31 22.283925486446883 25.51564665681651 29.654657402028278 22.54577045470833 32-33 22.315128597077738 25.708322691561424 29.43803820364349 22.538510507717348 34-35 22.776577216027544 25.621876797586555 29.18516270862487 22.416383277761025 36-37 22.8615200936831 25.38173118561574 29.04686899613923 22.709879724561926 38-39 22.607490073616862 25.505887214330585 29.125510042267177 22.761112669785373 40-41 22.744242960229634 25.346794698301 29.193881012918794 22.71508132855057 42-43 22.636041767048678 25.44420945735772 28.98599175393548 22.933757021658117 44-45 22.651450781093054 25.610611932782657 28.939115491834556 22.798821794289736 46-47 23.147037028001243 26.01985581585416 28.044776984209275 22.788330171935325 48-49 23.160200388670464 25.926218178463635 27.742821832941843 23.170759599924065 50-51 23.04464737705618 26.21083576791476 27.780187793140886 22.96432906188818 52-53 23.276529821843532 26.07989442353788 27.622943820635896 23.020631933982692 54-55 22.877682868069066 26.14138158175619 27.68812859469251 23.29280695548223 56-57 22.953872380264773 26.39704281882157 27.607079455756068 23.042005345157584 58-59 23.037260927126063 25.91366406804226 27.591810502071617 23.45726450276006 60-61 22.75482291088517 26.023324713186994 28.093036588861715 23.12881578706612 62-63 22.634173899254073 26.056222058222584 28.18444775154461 23.125156290978733 64-65 22.465532121226907 26.014480930975804 28.09025384704526 23.429733100752024 66-67 22.45706953652482 26.16276676201687 27.953137103020914 23.427026598437394 68-69 22.453770236838196 25.842237478605274 28.19712500619446 23.50686727836207 70-71 22.52927316551208 25.96402174812174 27.889911474333733 23.616793612032446 72-73 22.643767851976264 26.141106518522978 27.5293913145573 23.685734314943456 74-75 22.592051574498193 26.38084985697469 27.500655659715655 23.526442908811457 76-77 22.8441549035379 26.175175636886223 27.436940353980276 23.543729105595602 78-79 22.846805869831723 26.299654787535758 27.453044090828694 23.400495251803825 80-81 22.85295402511796 26.547115048133996 27.26243490074723 23.33749602600082 82-83 22.791799069268162 26.44862677409321 27.22241959782138 23.53715455881725 84-85 22.868534127850594 26.211483803680235 27.249713339473153 23.670268728996017 86-87 22.871316869667048 26.173745250279033 27.205341949413565 23.749595930640353 88-89 22.860948297419444 26.374560098074497 27.041198301993813 23.723293302512243 90-91 22.842430805954763 26.478255913995707 27.02131009247474 23.658003187574785 92-93 22.742700830095696 25.950439749446502 27.18407112840562 24.122788292052185 94-95 22.710642119580136 25.883234628591644 27.32198838719908 24.08413486462914 96-97 22.864387790849044 25.79259569300018 27.174055992598667 24.16896052355211 98-99 22.768495819592736 25.861116627892482 26.906399512981505 24.463988039533277 100-101 22.63151414376717 25.830562979379874 27.17516146579647 24.362761411056486 102-103 22.474223424434456 25.57408344870911 27.224401824868714 24.727291301987716 104-105 22.502177621484595 25.71808140587624 27.191011235955052 24.588729736684105 106-107 22.545354879633077 25.786181742774023 26.99457784663052 24.673885530962387 108-109 22.555542298146094 25.74152651403534 26.9200551525176 24.782876035300966 110-111 22.512126392000013 25.95112694247188 27.009870372262316 24.526876293265794 112-113 22.325060839122454 26.048788706795072 27.177095213989034 24.44905524009344 114-115 22.297309660459756 26.122512305055594 26.850561427691407 24.729616606793243 116-117 22.135575995373785 25.99855678568039 27.110815301731282 24.755051917214544 118-119 21.980801368651537 26.349553541477338 26.76086565379947 24.908779436071654 120-121 22.120836120847557 26.48186162112297 26.84499133726349 24.55231092076598 122-123 22.05442235476959 26.476186264841534 26.708184395438263 24.76120698495062 124-125 21.994266024882293 26.573707806661123 26.63938816251676 24.79263800593982 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 177.0 1 146.5 2 251.0 3 389.0 4 254.0 5 183.0 6 453.5 7 1197.5 8 2146.5 9 2511.5 10 1945.0 11 1258.0 12 1130.5 13 1218.0 14 1378.5 15 1525.0 16 1571.5 17 1674.5 18 1823.5 19 1930.0 20 2140.0 21 2524.0 22 2969.0 23 3580.0 24 4515.0 25 5697.5 26 7039.0 27 8617.5 28 10302.5 29 12284.0 30 14588.0 31 16868.5 32 19879.0 33 21517.5 34 22247.5 35 23474.0 36 25665.0 37 28563.5 38 30689.0 39 32052.0 40 33018.5 41 35160.5 42 37141.5 43 38717.0 44 41507.5 45 44368.5 46 45116.0 47 45689.0 48 46020.5 49 45635.5 50 46275.0 51 46324.0 52 46607.5 53 48537.0 54 50821.0 55 48844.5 56 44286.0 57 41303.5 58 39299.5 59 35516.5 60 29237.0 61 24132.5 62 19891.5 63 15031.5 64 11668.0 65 9493.5 66 6833.5 67 5431.0 68 3972.5 69 2263.5 70 1506.0 71 1071.0 72 776.5 73 550.5 74 437.0 75 392.5 76 265.0 77 117.0 78 50.0 79 21.5 80 10.5 81 9.0 82 6.0 83 3.5 84 2.5 85 1.5 86 1.5 87 1.0 88 0.5 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02744622065541575 2 0.0 3 0.0 4 0.0 5 0.056340991845422884 6 0.017840043426020236 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 3.049580072823972E-4 22-23 0.0 24-25 0.0 26-27 1.9059875455149826E-4 28-29 1.524790036411986E-4 30-31 1.524790036411986E-4 32-33 7.62395018205993E-5 34-35 1.1435925273089896E-4 36-37 0.0 38-39 0.0 40-41 7.62395018205993E-5 42-43 0.0 44-45 3.811975091029965E-5 46-47 0.0 48-49 3.811975091029965E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 1.1435925273089896E-4 58-59 3.811975091029965E-5 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 7.62395018205993E-5 70-71 1.9059875455149826E-4 72-73 1.1435925273089896E-4 74-75 0.0 76-77 7.62395018205993E-5 78-79 0.0 80-81 3.811975091029965E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 3.811975091029965E-5 92-93 0.0 94-95 0.0 96-97 3.811975091029965E-5 98-99 1.1435925273089896E-4 100-101 3.811975091029965E-5 102-103 0.0 104-105 0.001410430783681087 106-107 0.0 108-109 0.0015629097873222858 110-111 0.004116933098312362 112-113 0.0 114-115 0.0 116-117 3.811975091029965E-5 118-119 0.0 120-121 2.668382563720975E-4 122-123 5.717962636544948E-4 124-125 3.811975091029965E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1311656.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.60435937790923 #Duplication Level Percentage of deduplicated Percentage of total 1 82.54616928013249 58.28119401124206 2 10.955877487235668 15.470654228182646 3 3.216219899418541 6.812374368507893 4 1.3312617095539405 3.7597152066958497 5 0.6470831748894089 2.2843446513645156 6 0.3779076884223223 1.6009158147026754 7 0.22130996032721523 1.0937813580997477 8 0.13711124952010362 0.7744521548697265 9 0.11190068795102588 0.7110608748056557 >10 0.4261420330548709 5.135864676657595 >50 0.02007496365048721 0.9676720940748722 >100 0.008029430562635045 1.2260777692748261 >500 1.8248705627242514E-4 0.10578077378060416 >1k 5.474611688172754E-4 1.1110202342389155 >5k 1.8248705627242514E-4 0.66509178350241 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5162 0.3935483083979336 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4692 0.3577157425422519 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2834 0.2160627481595784 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.62395018205993E-5 2 0.0 0.0 0.0 0.0 7.62395018205993E-5 3 0.0 0.0 0.0 0.0 7.62395018205993E-5 4 0.0 0.0 0.0 0.0 7.62395018205993E-5 5 0.0 0.0 0.0 0.0 7.62395018205993E-5 6 0.0 0.0 0.0 0.0 1.524790036411986E-4 7 0.0 0.0 0.0 0.0 1.524790036411986E-4 8 0.0 0.0 0.0 0.0 1.524790036411986E-4 9 0.0 0.0 0.0 0.0 2.2871850546179792E-4 10-11 0.0 0.0 0.0 0.0 2.2871850546179792E-4 12-13 0.0 0.0 0.0 0.0 2.2871850546179792E-4 14-15 0.0 0.0 0.0 0.0 3.049580072823972E-4 16-17 0.0 0.0 0.0 7.62395018205993E-5 3.049580072823972E-4 18-19 0.0 0.0 0.0 7.62395018205993E-5 3.811975091029965E-4 20-21 0.0 0.0 0.0 1.9059875455149826E-4 4.1931726001329616E-4 22-23 0.0 0.0 0.0 5.717962636544948E-4 4.955567618338955E-4 24-25 0.0 0.0 0.0 0.0010673530254883903 5.336765127441952E-4 26-27 0.0 0.0 0.0 0.0016772690400531846 5.336765127441952E-4 28-29 0.0 0.0 0.0 0.005069926871069854 5.336765127441952E-4 30-31 0.0 0.0 0.0 0.015247900364119861 5.717962636544948E-4 32-33 0.0 0.0 0.0 0.03587068560659197 6.099160145647944E-4 34-35 0.0 0.0 0.0 0.06682392334575529 6.861555163853937E-4 36-37 0.0 0.0 0.0 0.11969601785834091 6.861555163853937E-4 38-39 7.62395018205993E-5 0.0 0.0 0.1976127887189934 6.861555163853937E-4 40-41 7.62395018205993E-5 0.0 0.0 0.28544069481632384 6.861555163853937E-4 42-43 7.62395018205993E-5 0.0 0.0 0.37128637386631863 6.861555163853937E-4 44-45 7.62395018205993E-5 0.0 0.0 0.4743240605768586 7.242752672956933E-4 46-47 7.62395018205993E-5 0.0 0.0 0.6017965076209006 7.62395018205993E-4 48-49 7.62395018205993E-5 0.0 0.0 0.7368547850960923 7.62395018205993E-4 50-51 7.62395018205993E-5 0.0 0.0 0.8939462785974371 7.62395018205993E-4 52-53 7.62395018205993E-5 0.0 0.0 1.0346462792073532 7.62395018205993E-4 54-55 7.62395018205993E-5 0.0 0.0 1.1878876778667578 7.62395018205993E-4 56-57 7.62395018205993E-5 0.0 0.0 1.3625142567868405 7.62395018205993E-4 58-59 7.62395018205993E-5 0.0 0.0 1.5605463627658471 7.62395018205993E-4 60-61 7.62395018205993E-5 0.0 0.0 1.7921619692968278 7.62395018205993E-4 62-63 7.62395018205993E-5 0.0 0.0 2.016725421909403 7.62395018205993E-4 64-65 7.62395018205993E-5 0.0 0.0 2.2388492104637194 7.62395018205993E-4 66-67 7.62395018205993E-5 0.0 0.0 2.4866275913806666 7.62395018205993E-4 68-69 7.62395018205993E-5 0.0 0.0 2.7758040217862003 7.62395018205993E-4 70-71 7.62395018205993E-5 0.0 0.0 3.0932271876162654 9.148740218471917E-4 72-73 7.62395018205993E-5 0.0 0.0 3.423229871246729 0.0011435925273089896 74-75 7.62395018205993E-5 0.0 0.0 3.7432832998896055 0.0011435925273089896 76-77 7.62395018205993E-5 0.0 0.0 4.090782949187897 0.0011435925273089896 78-79 7.62395018205993E-5 0.0 0.0 4.475449355623731 0.0011435925273089896 80-81 1.524790036411986E-4 0.0 0.0 4.892098233073305 0.0011435925273089896 82-83 1.524790036411986E-4 0.0 0.0 5.328798099501698 0.0011435925273089896 84-85 1.524790036411986E-4 0.0 0.0 5.762067188348165 0.0011435925273089896 86-87 1.524790036411986E-4 0.0 0.0 6.185501381459773 0.0011435925273089896 88-89 1.524790036411986E-4 0.0 0.0 6.65582286819105 0.0011435925273089896 90-91 2.2871850546179792E-4 0.0 0.0 7.185191849082381 0.0011435925273089896 92-93 2.2871850546179792E-4 0.0 0.0 7.740024823581793 0.0011435925273089896 94-95 2.2871850546179792E-4 0.0 0.0 8.293714205553895 0.0011435925273089896 96-97 2.2871850546179792E-4 0.0 0.0 8.821863354416097 0.0011435925273089896 98-99 2.2871850546179792E-4 0.0 0.0 9.387522338174033 0.0011435925273089896 100-101 2.2871850546179792E-4 0.0 0.0 10.01939532926316 0.0011435925273089896 102-103 2.2871850546179792E-4 0.0 0.0 10.677227870722202 0.0012198320291295888 104-105 2.2871850546179792E-4 0.0 0.0 11.335822807199449 0.0012198320291295888 106-107 2.2871850546179792E-4 0.0 0.0 11.96327390718298 0.0012198320291295888 108-109 2.2871850546179792E-4 0.0 0.0 12.622783717682076 0.0012198320291295888 110-111 2.2871850546179792E-4 0.0 0.0 13.32807534902444 0.0013341912818604878 112-113 2.2871850546179792E-4 0.0 0.0 14.084828644095708 0.0013723110327707874 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3030 0.0 82.940414 1 GTATCAA 5330 0.0 54.859356 1 ATCAACG 6955 0.0 41.7769 3 TATCAAC 6985 0.0 41.512234 2 TCAACGC 6985 0.0 41.512234 4 CAACGCA 7190 0.0 40.328644 5 AACGCAG 7280 0.0 39.89056 6 GTACATG 6870 0.0 37.27418 1 TACATGG 7095 0.0 35.74956 2 TGGTATC 1080 0.0 35.283344 2 GTGGTAT 1190 0.0 35.03059 1 ACGCAGA 8510 0.0 34.188354 7 ACATGGG 7255 0.0 34.140465 3 CGCAGAG 8640 0.0 33.742805 8 GCAGAGT 9645 0.0 29.980091 9 CATGGGA 4790 0.0 29.956778 4 GAGTACT 6000 0.0 29.550436 12-13 CAGAGTA 9340 0.0 28.41102 10-11 CATGGGG 3160 0.0 27.509285 4 GTACTTT 6605 0.0 26.753618 14-15 >>END_MODULE