FastQCFastQC Report
Thu 26 May 2016
SRR1364398_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364398_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1676589
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15000.058.346371
GTATCAA23950.045.2432371
ATCAACG25600.041.6145633
CAACGCA26850.039.6760185
TCAACGC27900.038.183974
AACGCAG27900.038.1805536
GTACATG69400.038.004241
TACATGG72100.036.4028662
TATCAAC30450.035.3772052
ACATGGG72800.035.071813
ACGCAGA34650.030.7336127
CGCAGAG35900.029.8274428
CATGGGG36250.028.4034044
CATGGGA44350.027.3759254
ACAATCG1200.001289475524.7871558
GAGTACA50400.022.444551
GCAGAGT46700.022.2925179
CCCTATA5100.022.1725522
ATGGGAG24200.022.1333145
AGTACAT51300.021.2307822
Produced by FastQC (version 0.11.5)