FastQCFastQC Report
Thu 26 May 2016
SRR1364398_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364398_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1676589
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25770.1537049330515708No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23990.14308813907284373No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25250.067.946581
GTATCAA44500.053.2794271
ATCAACG52600.043.474973
TCAACGC54500.041.850064
CAACGCA54850.041.5830155
TATCAAC56800.040.4699632
AACGCAG56400.040.413686
GTACATG67350.032.9919321
ACGCAGA69300.032.7961047
CGCAGAG69350.032.772468
TACATGG71250.030.757912
ACATGGG71150.029.7967533
GCAGAGT81800.027.711749
CATGGGG34250.025.9071454
GAGTACT44800.025.23293312-13
TGGTATC13700.025.211652
GTGGTAT14650.024.8043981
CGTATAG1451.3295932E-424.650161
CAGAGTA79750.024.3581710-11
AGAGTAC75400.024.3036510-11
Produced by FastQC (version 0.11.5)