FastQCFastQC Report
Thu 26 May 2016
SRR1364397_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364397_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1344338
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19650.048.1740571
GGTATCA13000.044.880881
ATCAACG21400.041.163613
TCAACGC21400.040.885484
CAACGCA22500.038.6220975
GTACATG55100.037.169391
AACGCAG23450.037.054696
TACATGG57300.035.3174862
TATCAAC25800.035.0662572
GTATACG851.7045895E-435.0210951
ACATGGG58550.033.546913
ACGCAGA27900.030.7087547
CGCAGAG29700.028.8454728
CATGGGA33800.028.7036024
CATGGGG30400.027.4106884
TATTCCG2558.785719E-1025.6754475
GAGTACA41250.023.2370281
GTAGGAC2853.1886884E-922.9727693
GTGGTAT9650.022.8272231
CCGTTGC1852.6930955E-522.5111649
Produced by FastQC (version 0.11.5)