FastQCFastQC Report
Thu 26 May 2016
SRR1364397_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364397_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1344338
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30250.22501781546010008No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29180.21705850760746181No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16160.12020786439124684No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25300.070.614251
GTATCAA43000.052.626781
ATCAACG53650.041.7188153
TCAACGC55100.040.620954
TATCAAC56550.039.6846542
CAACGCA56150.039.6493155
AACGCAG57350.038.805256
GTGGTAT11300.037.417221
ACGCAGA66550.033.34767
CGCAGAG66650.033.2082948
TGGTATC11550.032.9846462
GTACATG55400.032.4630131
TACATGG57400.030.5931322
ACATGGG57900.029.6092763
GCAGAGT75650.028.9429449
AATACCG1107.731419E-427.0577165
TCGATCG450.00953259726.44364412-13
CAGAGTA73750.026.38089410-11
GAGTACT47550.026.33936912-13
AGAGTAC70000.025.96671310-11
Produced by FastQC (version 0.11.5)