FastQCFastQC Report
Thu 26 May 2016
SRR1364396_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364396_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2147173
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18450.054.8537751
GTATCAA33450.050.366791
ATCAACG36550.044.1228873
TCAACGC37650.043.1488844
CAACGCA37750.042.7193075
AACGCAG39700.040.7670976
TATCAAC42200.038.3573462
GTACATG102900.035.927771
ACATGGG104250.034.0784843
TACATGG108100.034.021612
ACGCAGA49600.032.6263167
CGCAGAG50600.031.747118
CATGGGA71650.030.4804764
CATGGGG40150.026.3819164
GAGTACA78800.024.9311141
TAAGGTG9450.024.558665
GCAGAGT65300.024.053699
ATGGGAT32300.023.9503975
ATGGGAG34450.023.319355
AGTACAT81550.022.6219392
Produced by FastQC (version 0.11.5)