FastQCFastQC Report
Thu 26 May 2016
SRR1364396_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364396_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2147173
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34170.15913948247300055No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31000.14437588401120915No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34500.072.3256761
GTATCAA59600.053.15731
ATCAACG73700.042.3311583
CAACGCA74950.041.7046055
TCAACGC75500.041.4007954
TATCAAC78550.040.248032
AACGCAG78600.039.8952646
GTACATG105850.036.1757621
ACATGGG105650.034.8832933
TACATGG111950.034.356172
ACGCAGA93800.033.4272467
CGCAGAG93900.033.3282858
CATGGGA72750.029.8714454
GCAGAGT111100.028.0078939
CATGGGG43150.027.0440434
GTGGTAT17100.026.119371
GAGTACT56350.025.34020812-13
CAGAGTA106750.025.30351310-11
ATGGGAT33850.024.2726275
AGAGTAC103800.023.72989510-11
Produced by FastQC (version 0.11.5)