FastQCFastQC Report
Thu 26 May 2016
SRR1364395_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364395_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1323758
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14600.050.554481
GTATCAA21050.045.2437671
TCAACGC23200.038.9954224
ATCAACG23700.038.423873
GTACATG60800.037.104491
CAACGCA25000.036.1877565
AACGCAG25600.035.3342636
TACATGG64950.034.272872
TATCAAC27650.033.7957842
ACATGGG64350.033.5750053
CATGGGG31000.028.9916734
ACGCAGA31450.028.9422267
CGCAGAG32050.027.6579118
CATGGGA38700.027.222024
CCGATAG900.00951439626.4530353
ATGGGAT18450.022.9044555
ATGGGGA18200.022.8920485
CTACACG4700.022.7946364
GAGTACA49200.022.7448771
GCAGAGT40250.021.8754489
Produced by FastQC (version 0.11.5)