##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364395_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1323758 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.017122464982272 31.0 31.0 34.0 28.0 34.0 2 31.02007088909 33.0 31.0 34.0 27.0 34.0 3 31.09123873094629 33.0 31.0 34.0 27.0 34.0 4 34.472233595566564 37.0 35.0 37.0 31.0 37.0 5 34.531380358041275 37.0 35.0 37.0 31.0 37.0 6 34.589914470771845 37.0 35.0 37.0 31.0 37.0 7 34.484822754612246 37.0 35.0 37.0 31.0 37.0 8 34.43243553580035 37.0 35.0 37.0 31.0 37.0 9 35.94255596566744 39.0 37.0 39.0 31.0 39.0 10-11 35.86853337241399 39.0 37.0 39.0 30.0 39.0 12-13 35.91665017321897 39.0 37.0 39.0 30.5 39.0 14-15 37.23903613802523 40.0 38.0 41.0 31.0 41.0 16-17 37.26746165084555 40.0 38.0 41.0 31.0 41.0 18-19 37.27424385726092 40.0 38.0 41.0 31.0 41.0 20-21 37.30144671458076 40.0 38.0 41.0 31.0 41.0 22-23 37.278811535038884 40.0 38.0 41.0 31.0 41.0 24-25 37.311030792637325 40.0 38.0 41.0 31.0 41.0 26-27 37.28425059565268 40.0 38.0 41.0 30.0 41.0 28-29 37.29188643241439 40.0 38.0 41.0 30.0 41.0 30-31 37.20800025382283 40.0 37.5 41.0 30.0 41.0 32-33 37.17094967509167 40.0 37.0 41.0 30.0 41.0 34-35 37.16338295972527 40.0 37.0 41.0 30.0 41.0 36-37 37.10280579985164 40.0 37.0 41.0 30.0 41.0 38-39 37.01547223888354 40.0 37.0 41.0 30.0 41.0 40-41 36.952574035435475 40.0 37.0 41.0 30.0 41.0 42-43 36.828214824764046 40.0 37.0 41.0 30.0 41.0 44-45 36.71559340906721 40.0 37.0 41.0 30.0 41.0 46-47 36.60706375334465 40.0 36.0 41.0 30.0 41.0 48-49 36.46815278925604 40.0 36.0 41.0 30.0 41.0 50-51 36.352887385760845 40.0 36.0 41.0 29.0 41.0 52-53 36.20460839519006 40.0 35.0 41.0 29.0 41.0 54-55 36.0244448003336 39.0 35.0 41.0 29.0 41.0 56-57 35.83892486391017 39.0 35.0 41.0 28.0 41.0 58-59 35.6346681190973 39.0 35.0 41.0 28.0 41.0 60-61 35.39841232309833 38.5 35.0 41.0 28.0 41.0 62-63 35.13746508047544 38.0 34.0 40.0 28.0 41.0 64-65 34.81397392876946 37.5 34.0 40.0 28.0 41.0 66-67 34.51568338019487 37.0 34.0 40.0 27.0 41.0 68-69 34.203515672804244 36.5 34.0 39.5 26.5 41.0 70-71 33.847847945017136 36.0 33.5 39.0 26.0 41.0 72-73 33.48405297645038 35.5 33.0 39.0 26.0 40.5 74-75 33.13641730588219 35.0 33.0 37.5 26.0 40.0 76-77 32.8976198821839 35.0 33.0 37.0 26.0 39.0 78-79 32.72544566302904 35.0 33.0 37.0 26.0 39.0 80-81 32.4469623601897 35.0 33.0 36.0 26.0 39.0 82-83 32.15023440840395 35.0 33.0 36.0 25.5 37.0 84-85 31.89808409089879 35.0 33.0 35.5 25.0 37.0 86-87 31.680030262328913 35.0 33.0 35.0 25.0 36.5 88-89 31.419974799019155 35.0 32.5 35.0 24.0 36.0 90-91 31.293771595714624 35.0 32.0 35.0 24.0 36.0 92-93 31.148944520070888 35.0 32.0 35.0 23.5 36.0 94-95 31.048692434719943 35.0 32.0 35.0 23.0 35.5 96-97 30.94192782970906 35.0 32.0 35.0 22.5 35.0 98-99 30.819780881399772 35.0 32.0 35.0 20.0 35.0 100-101 30.732352136870936 35.0 32.0 35.0 20.0 35.0 102-103 29.737438791682465 33.5 30.0 34.5 18.5 35.0 104-105 30.432602862456733 34.5 31.5 35.0 19.0 35.0 106-107 30.762521170788013 35.0 32.0 35.0 20.0 35.0 108-109 30.798106980278874 35.0 33.0 35.0 19.5 35.0 110-111 30.733414642253344 35.0 32.5 35.0 19.0 35.0 112-113 30.651662161815075 35.0 32.0 35.0 18.5 35.0 114-115 30.560176029153368 35.0 32.0 35.0 17.0 35.0 116-117 30.431206459186647 35.0 32.0 35.0 8.5 35.0 118-119 30.26785220561462 35.0 32.0 35.0 2.0 35.0 120-121 30.102286822818066 35.0 32.0 35.0 2.0 35.0 122-123 29.928562471388275 35.0 32.0 35.0 2.0 35.0 124-125 29.12708516209156 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24336030452183977 1101 2 0.21358851373374677 1101 3 0.32239543150732786 1101 4 0.5100304189865952 1101 5 0.4245529749831789 1101 6 0.43618886653914046 1101 7 0.47825414607768124 1101 8 0.2998805351144611 1101 9 0.3986858031142049 1101 10-11 0.5099943427031377 1101 12-13 0.6072680617620207 1101 14-15 0.6993091443955066 1101 16-17 0.6495009900488498 1101 18-19 0.5443518098348363 1101 20-21 0.656006420164907 1101 22-23 0.7214215263771635 1101 24-25 0.6994985704366385 1101 26-27 0.7821036156504704 1101 28-29 0.7981824223982912 1101 30-31 0.8179645942045113 1101 32-33 0.6717345534128683 1101 34-35 0.7438363029031123 1101 36-37 0.65610213474109 1101 38-39 0.7221855686479159 1101 40-41 0.6086799858352023 1101 42-43 0.7629995762162451 1101 44-45 0.741964706907936 1101 46-47 0.7862916917639708 1101 48-49 0.6050442523632711 1101 50-51 0.5955496813144876 1101 52-53 0.5838147405418113 1101 54-55 0.6080868972231812 1101 56-57 0.5671737287446845 1101 58-59 0.561216329232181 1101 60-61 0.583874989263073 1101 62-63 0.5387621261562643 1101 64-65 0.510533523222584 1101 66-67 0.5914646564244634 1101 68-69 0.4595019662991291 1101 70-71 0.44833308363120494 1101 72-73 0.5807463246903808 1101 74-75 0.5314092055827686 1101 76-77 0.46304587897198246 1101 78-79 0.5258543589110332 1101 80-81 0.5818686087215781 1101 82-83 0.5519945754166073 1101 84-85 0.5385664915636426 1101 86-87 0.5557518414768303 1101 88-89 0.5539143474291599 1101 90-91 0.48358868867585514 1101 92-93 0.5601454388999514 1101 94-95 0.4825363392000419 1101 96-97 0.39783926822882876 1101 98-99 0.2681383249594056 1101 100-101 0.37263854340412905 1101 102-103 0.12579691274832783 1101 104-105 0.3514407704655831 1101 106-107 0.3778869700437504 1101 108-109 0.47094582824209397 1101 110-111 0.4451698207621817 1101 112-113 0.5045310050524492 1101 114-115 0.387541490100503 1101 116-117 0.4206131739758341 1101 118-119 0.5251182867507502 1101 120-121 0.3902579532067918 1101 122-123 0.3077001994243318 1101 124-125 0.2593990041879195 1102 1 0.17392158417672476 1102 2 0.21552153214226522 1102 3 0.2803169277395092 1102 4 0.43643925815759843 1102 5 0.40755061167146067 1102 6 0.4042247316515457 1102 7 0.25319203692514236 1102 8 0.28893144123404113 1102 9 0.48297789633708277 1102 10-11 0.47330537268335604 1102 12-13 0.42556350599033976 1102 14-15 0.4003883167136735 1102 16-17 0.5202418703893485 1102 18-19 0.4825115906163546 1102 20-21 0.49061586845955674 1102 22-23 0.517580113122257 1102 24-25 0.5519448319196059 1102 26-27 0.502071673800593 1102 28-29 0.4669483279813704 1102 30-31 0.5083542517581776 1102 32-33 0.5414455085594696 1102 34-35 0.4237987760441655 1102 36-37 0.3429023922567751 1102 38-39 0.1997605191944487 1102 40-41 0.3086603144978568 1102 42-43 0.30149885951927047 1102 44-45 0.282085431143301 1102 46-47 0.23526259194087373 1102 48-49 0.3466381259109568 1102 50-51 0.26128372483353957 1102 52-53 0.28729587944346235 1102 54-55 0.30024092241900036 1102 56-57 0.33225435371545586 1102 58-59 0.23433166379622605 1102 60-61 0.194001876267059 1102 62-63 0.26832484470757834 1102 64-65 0.19518575323690612 1102 66-67 0.13894342376068636 1102 68-69 0.08103396454934142 1102 70-71 -0.01347329538456421 1102 72-73 0.025871684758385527 1102 74-75 0.0987689544499517 1102 76-77 0.1414664219008941 1102 78-79 0.314745143095827 1102 80-81 0.23227991850249907 1102 82-83 0.2287729055814367 1102 84-85 0.23489414176049195 1102 86-87 0.21404200333205026 1102 88-89 0.3042632041530453 1102 90-91 0.20796986692018038 1102 92-93 0.33712612247204277 1102 94-95 0.19627931323112335 1102 96-97 0.2719484867952673 1102 98-99 0.281349760196683 1102 100-101 0.2477780998498602 1102 102-103 -0.13156635770091896 1102 104-105 0.03854132538988253 1102 106-107 0.10887592933370982 1102 108-109 0.21269597199417234 1102 110-111 0.180519626662484 1102 112-113 0.03822401663790487 1102 114-115 0.13276341855186047 1102 116-117 0.06566920289682798 1102 118-119 0.06199048908764837 1102 120-121 -0.024851009494703646 1102 122-123 -0.09599195927796345 1102 124-125 -0.03864803094261404 1103 1 0.045325044798183 1103 2 0.16753466817845108 1103 3 0.04525255848457377 1103 4 0.06886091220032142 1103 5 -0.05173174839695349 1103 6 0.0336622161017317 1103 7 -0.008523457550559499 1103 8 0.030867851840596927 1103 9 0.32275345465209426 1103 10-11 0.2796108987663217 1103 12-13 0.20562429390706427 1103 14-15 0.17819110435593188 1103 16-17 0.058851708693602234 1103 18-19 0.10836982875414947 1103 20-21 0.10219282316176503 1103 22-23 0.19771319721230185 1103 24-25 0.10197325391058598 1103 26-27 0.2907159587769286 1103 28-29 0.16997492137006986 1103 30-31 0.32521356876015517 1103 32-33 0.308947067724624 1103 34-35 0.28365911501057184 1103 36-37 0.13957219398898246 1103 38-39 0.1095417563292429 1103 40-41 0.2581436423085606 1103 42-43 0.12809417276363888 1103 44-45 -0.008066139792305194 1103 46-47 0.11444472474492784 1103 48-49 0.048673436120672875 1103 50-51 0.04385033381947068 1103 52-53 0.03989904373985809 1103 54-55 0.012461848590994862 1103 56-57 0.014865506292736086 1103 58-59 0.05635588970838512 1103 60-61 -0.10573995499054689 1103 62-63 -7.583327092390846E-4 1103 64-65 0.11158925848563683 1103 66-67 -0.04592126636430294 1103 68-69 -0.020998472780028976 1103 70-71 -0.109731522142404 1103 72-73 -0.08372800307387251 1103 74-75 -0.07028736293025162 1103 76-77 -0.036734859347951954 1103 78-79 0.09726308046815291 1103 80-81 0.015979223321238578 1103 82-83 -0.05775381555280745 1103 84-85 -0.10365374831808793 1103 86-87 -0.12927389189651706 1103 88-89 -0.06860152055807944 1103 90-91 0.09819529598346577 1103 92-93 0.06036914695121709 1103 94-95 -0.011126470239602781 1103 96-97 -0.061807689897474205 1103 98-99 -0.03712590064947108 1103 100-101 -0.118174697889323 1103 102-103 -0.4319692303581313 1103 104-105 -0.24111600413674594 1103 106-107 -0.20174059078816597 1103 108-109 -0.2083979985272819 1103 110-111 -0.2583380882206008 1103 112-113 -0.23318033652890335 1103 114-115 -0.38250775346059385 1103 116-117 -0.3660427102406594 1103 118-119 -0.30394125015968854 1103 120-121 -0.4307733825942819 1103 122-123 -0.35971568079042626 1103 124-125 -0.4569166770517299 1104 1 0.1345785540305009 1104 2 0.17064432642382243 1104 3 0.2403014037154918 1104 4 0.22180652908870258 1104 5 0.10656924103485466 1104 6 0.17709527016518223 1104 7 0.3217666332283571 1104 8 0.25903058864223993 1104 9 0.3339539479640834 1104 10-11 0.34827149406689983 1104 12-13 0.27475033900925894 1104 14-15 0.327371545528905 1104 16-17 0.375513623668553 1104 18-19 0.2976353792052322 1104 20-21 0.2754905296065644 1104 22-23 0.24114770918117046 1104 24-25 0.2955606839581222 1104 26-27 0.40021464450219923 1104 28-29 0.34025152382410084 1104 30-31 0.3703592297096705 1104 32-33 0.4392932608636073 1104 34-35 0.34594785371721315 1104 36-37 0.2551117377833805 1104 38-39 0.18398564640965986 1104 40-41 0.2854345516743635 1104 42-43 0.2527691138534607 1104 44-45 0.2573269115065244 1104 46-47 0.3500592480746505 1104 48-49 0.36952790908246413 1104 50-51 0.2551961317040963 1104 52-53 0.18717340857907772 1104 54-55 0.16843639896216445 1104 56-57 0.06636628492221774 1104 58-59 0.118689445507286 1104 60-61 0.019240336288156357 1104 62-63 0.09198551007023781 1104 64-65 0.08141574614311509 1104 66-67 0.022890519127052755 1104 68-69 -0.015423334495004326 1104 70-71 -0.111412433084908 1104 72-73 -0.14183174330320725 1104 74-75 -0.1725505180967417 1104 76-77 -0.10576101093123924 1104 78-79 -0.033948946495534926 1104 80-81 -0.04395121839170457 1104 82-83 -0.053943012797695644 1104 84-85 -0.08777742837372315 1104 86-87 -0.13031602428296551 1104 88-89 -0.1036700853095951 1104 90-91 -0.2745390110146957 1104 92-93 -0.17090945769291466 1104 94-95 -0.1736300070101393 1104 96-97 -0.2712547009753976 1104 98-99 -0.28915103925847774 1104 100-101 -0.2599273809752418 1104 102-103 -0.4428203663364272 1104 104-105 -0.38605610042329985 1104 106-107 -0.3792727025047604 1104 108-109 -0.40006342327452415 1104 110-111 -0.4078136202480813 1104 112-113 -0.44203176624814233 1104 114-115 -0.5464659558524261 1104 116-117 -0.49359485465752684 1104 118-119 -0.40333665138605923 1104 120-121 -0.4784506692919166 1104 122-123 -0.46248829290335536 1104 124-125 -0.5030790920342447 1105 1 -0.0460925609506333 1105 2 -0.09283606512222065 1105 3 -0.18604745784729104 1105 4 0.031256796570730216 1105 5 0.014289809662820119 1105 6 0.0632228889745079 1105 7 0.1369934459281339 1105 8 0.06046935437203871 1105 9 0.16053722077980837 1105 10-11 0.034898339563312675 1105 12-13 0.1493814390973256 1105 14-15 0.18435638281420097 1105 16-17 0.1534131981595479 1105 18-19 0.1179035540522051 1105 20-21 0.12756845602147138 1105 22-23 0.17062271868869772 1105 24-25 0.16038008062951548 1105 26-27 0.25578618578983736 1105 28-29 0.16742306770356663 1105 30-31 0.1360293453402619 1105 32-33 0.30333298965831546 1105 34-35 0.1703159825591669 1105 36-37 0.041907650464565904 1105 38-39 0.1226480767604059 1105 40-41 0.14859766811290598 1105 42-43 0.1820097370295244 1105 44-45 0.058629107635425726 1105 46-47 0.11377103537696343 1105 48-49 -0.06575168228533812 1105 50-51 -0.0800880950552667 1105 52-53 0.024465432764849027 1105 54-55 0.08046364509598192 1105 56-57 0.0078019753438525186 1105 58-59 0.019854174862715013 1105 60-61 0.011196187423301751 1105 62-63 0.07261256391006299 1105 64-65 0.07482215085865818 1105 66-67 0.04910976417628632 1105 68-69 -0.07424034767795007 1105 70-71 -0.08417215581871318 1105 72-73 -0.1410732586675323 1105 74-75 -0.02796742172495925 1105 76-77 0.010852107824995016 1105 78-79 0.12143423839727063 1105 80-81 0.11844125173868036 1105 82-83 0.1676871447607624 1105 84-85 0.15212364838287584 1105 86-87 0.07789579616489206 1105 88-89 0.10369963900422974 1105 90-91 0.07183975131582088 1105 92-93 0.03254373457166082 1105 94-95 -0.013657909076776775 1105 96-97 -0.0438426400852876 1105 98-99 -0.039555265339981105 1105 100-101 -0.0703631675937153 1105 102-103 -0.20993754654300645 1105 104-105 -0.08996460776242188 1105 106-107 -0.04913974153126688 1105 108-109 0.01608346480768219 1105 110-111 0.05631567627392897 1105 112-113 0.030048472878252852 1105 114-115 0.035710440235305896 1105 116-117 -0.03238273963801319 1105 118-119 -0.03811975742736706 1105 120-121 -0.10160467444467969 1105 122-123 -0.08289059504814489 1105 124-125 -0.1686797017128434 1106 1 0.054995421273893896 1106 2 0.20095465042598804 1106 3 0.13481388413167394 1106 4 0.23862044114235914 1106 5 0.14098405291854732 1106 6 0.06803891290856257 1106 7 0.12197663511338419 1106 8 0.08183708416048319 1106 9 0.1726240502699028 1106 10-11 0.34804118784531113 1106 12-13 0.222881327425398 1106 14-15 0.3169325979686235 1106 16-17 0.30971575931086903 1106 18-19 0.2596522489412294 1106 20-21 0.20713839528844602 1106 22-23 0.1777111976219743 1106 24-25 0.17553907619740272 1106 26-27 0.2664351610216755 1106 28-29 0.30679363818580896 1106 30-31 0.37121593250351737 1106 32-33 0.2787178951648599 1106 34-35 0.22097764812411214 1106 36-37 0.17990701969948475 1106 38-39 0.23889005245069228 1106 40-41 0.17583774075343683 1106 42-43 0.25593017709446286 1106 44-45 0.0712407826918735 1106 46-47 0.027107740127142677 1106 48-49 0.03395084270078996 1106 50-51 -0.0013074875049454704 1106 52-53 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112-113 0.5170221503337515 2204 114-115 0.4476846036592761 2204 116-117 0.5506574342539672 2204 118-119 0.5104476822863724 2204 120-121 0.5177344782157114 2204 122-123 0.5906653184903377 2204 124-125 0.6268802162058371 2205 1 0.10517885983276187 2205 2 -0.004572283510452024 2205 3 0.1958418692963626 2205 4 0.20494493224415322 2205 5 0.06034837565948692 2205 6 0.11379481637428057 2205 7 0.21380093466259353 2205 8 0.2794880229735526 2205 9 0.24635664491896136 2205 10-11 0.3993943553444268 2205 12-13 0.16717277801767239 2205 14-15 0.2422463487736053 2205 16-17 0.22437510384155956 2205 18-19 0.4063589310964346 2205 20-21 0.2571907512825007 2205 22-23 0.3305761925943571 2205 24-25 0.4176641081656314 2205 26-27 0.38865443891465645 2205 28-29 0.2813961023839653 2205 30-31 0.23145996433103733 2205 32-33 0.31533358214020524 2205 34-35 0.34188533005028177 2205 36-37 0.4211560709236295 2205 38-39 0.4186960451193116 2205 40-41 0.4143798920789479 2205 42-43 0.37415920414086656 2205 44-45 0.4523139752931087 2205 46-47 0.364148360572635 2205 48-49 0.30731178425619987 2205 50-51 0.4374534600075961 2205 52-53 0.3975529288338677 2205 54-55 0.41592028498430267 2205 56-57 0.46790112980023935 2205 58-59 0.42060828304931874 2205 60-61 0.476583070209486 2205 62-63 0.37571868224673466 2205 64-65 0.46215977286704657 2205 66-67 0.5036906300673678 2205 68-69 0.4508442609044536 2205 70-71 0.5616032807300257 2205 72-73 0.5652075422575606 2205 74-75 0.6444821458421259 2205 76-77 0.5519597285616626 2205 78-79 0.3469800660333462 2205 80-81 0.4221134810971918 2205 82-83 0.5229693771063282 2205 84-85 0.4484401313078621 2205 86-87 0.42032198485015826 2205 88-89 0.5035922578234668 2205 90-91 0.5231980727288637 2205 92-93 0.6128293433739138 2205 94-95 0.5374569624157743 2205 96-97 0.5383949057186861 2205 98-99 0.6543824915295069 2205 100-101 0.6226769940030081 2205 102-103 0.6207638346988773 2205 104-105 0.620872807715255 2205 106-107 0.621878405567891 2205 108-109 0.6998951787956784 2205 110-111 0.749861320546227 2205 112-113 0.7241207951016477 2205 114-115 0.7523897419150529 2205 116-117 0.761498011798583 2205 118-119 0.9055732679468278 2205 120-121 0.879930543723713 2205 122-123 1.0193372995117649 2205 124-125 1.102767329321967 2206 1 0.18745358377254462 2206 2 0.18739509662015408 2206 3 0.16131686028746728 2206 4 0.18754601821201788 2206 5 0.016687884044145562 2206 6 0.12949122720863926 2206 7 0.025466207884882408 2206 8 0.14310758917849853 2206 9 0.17799441675545324 2206 10-11 0.17444789831713337 2206 12-13 0.10478998302345133 2206 14-15 0.3035801047340243 2206 16-17 0.2553780928563114 2206 18-19 0.25531058112854055 2206 20-21 0.15883539846544892 2206 22-23 0.2522269750159367 2206 24-25 0.2835734148428273 2206 26-27 0.3484548445958282 2206 28-29 0.3165666640594509 2206 30-31 0.36199571562213606 2206 32-33 0.40724540314232627 2206 34-35 0.48515080825691825 2206 36-37 0.5539904950227594 2206 38-39 0.49671387304066883 2206 40-41 0.3738233871437089 2206 42-43 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0.8092595316010822 2207 108-109 0.7793817464674646 2207 110-111 0.8392459428870147 2207 112-113 0.8111372760095676 2207 114-115 0.8610180628912296 2207 116-117 0.8318280280465338 2207 118-119 0.9220762919531005 2207 120-121 0.9660115141579482 2207 122-123 0.9666077566463649 2207 124-125 1.0368182641443866 2208 1 0.30280568263023966 2208 2 0.3235300371842946 2208 3 0.31472589924321426 2208 4 0.44812025304376846 2208 5 0.28926914822474004 2208 6 0.3706658036226571 2208 7 0.3106893768598624 2208 8 0.4068018327685152 2208 9 0.2943560392390907 2208 10-11 0.4087928064504851 2208 12-13 0.3910394804240198 2208 14-15 0.4828333192079981 2208 16-17 0.30138011981170365 2208 18-19 0.4214318331948519 2208 20-21 0.3666087056722631 2208 22-23 0.4901335121571364 2208 24-25 0.45951188258614195 2208 26-27 0.5620641127393924 2208 28-29 0.403631095391205 2208 30-31 0.33946295058758835 2208 32-33 0.5062225839543046 2208 34-35 0.46612369741962567 2208 36-37 0.474920082170847 2208 38-39 0.5152762570288587 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120-121 -0.4632149961823906 2216 122-123 -0.3803265978509067 2216 124-125 -0.2829570167544908 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 24254.0 3 4395.0 4 2845.0 5 2244.0 6 2397.0 7 2751.0 8 3004.0 9 3411.0 10 3585.0 11 3977.0 12 4464.0 13 4432.0 14 4490.0 15 4390.0 16 4100.0 17 3681.0 18 3542.0 19 3432.0 20 3199.0 21 3391.0 22 4177.0 23 5616.0 24 8413.0 25 12483.0 26 14962.0 27 14519.0 28 16618.0 29 20592.0 30 24060.0 31 29367.0 32 37886.0 33 50507.0 34 71588.0 35 109120.0 36 211425.0 37 411647.0 38 188183.0 39 611.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.12889651685571 18.894635055445093 13.117130352833772 23.85933807486542 2 15.32357800722697 20.346268999503646 39.91771257270599 24.412440420563396 3 18.32189090205843 25.619235975970895 29.56528833718105 26.493584784789626 4 12.415161276675471 16.77283468611939 37.60093012854993 33.211073908655216 5 13.296323749982053 38.85311094336657 34.40916519890195 13.441400107749422 6 31.68052653651865 37.164576666301436 17.69073536023697 13.464161436942945 7 28.373523101562263 33.05978605747439 21.272776720152297 17.293914120811046 8 26.158879613101192 36.01506923098424 19.908185565779824 17.917865590134745 9 26.462918448840345 16.712042533454 19.64331849174849 37.18172052595716 10-11 24.586989908268663 27.3404786528041 27.867199191392377 20.20533224753486 12-13 24.990793169835136 24.92061351672206 27.79291789849333 22.295675414949475 14-15 22.171847769183525 26.35034632593845 25.090065895979087 26.38774000889894 16-17 22.48442217280897 29.688163948581174 25.770638331143225 22.05677554746662 18-19 22.580957078980507 28.529783034197365 27.076306300929854 21.812953585892274 20-21 23.226921557355315 27.73329999388105 26.982746833444505 22.057031615319126 22-23 23.092438345981666 27.729464146770027 26.67288885128551 22.505208655962797 24-25 22.860182903521643 27.867669166116464 26.887656203022004 22.384491727339892 26-27 22.614867149005764 28.14367435123125 26.699829840435275 22.54162865932771 28-29 22.54213671472057 27.93085435274539 27.019093012952155 22.507915919581887 30-31 22.51068172684392 27.656040841363517 27.109603280362847 22.72367415142972 32-33 22.415640640858044 27.944754190064415 26.882738630556304 22.756866538521244 34-35 22.969480642115204 27.854428706326722 26.51894239848914 22.657148253068932 36-37 23.05592225162086 27.43006177490968 26.560982657322057 22.953033316147405 38-39 22.56722537990385 27.63216937260341 26.86930219768598 22.93130304980676 40-41 22.82300612199264 27.432064314161565 27.010724063117564 22.734205500728233 42-43 22.770462700661 27.404041548630786 26.89922568460812 22.926270066100095 44-45 22.516539281620105 27.635963943138485 26.940662553328913 22.906834221912494 46-47 22.788472506660966 27.836528909762137 26.50395805272418 22.871040530852717 48-49 22.63593496696526 27.63431835728283 26.688979405601327 23.040767270150585 50-51 22.689947860560615 27.51224166350647 26.776079917930616 23.021730558002293 52-53 22.901322725042483 27.485175575800312 26.742358514048593 22.871143185108604 54-55 22.567658782695275 27.524713506146885 26.839938521718537 23.067689189439303 56-57 22.539971520409743 27.805145211916106 26.86127720008612 22.793606067588033 58-59 22.739393948550667 27.276092370985737 26.781587748130498 23.202925932333095 60-61 22.541262714415595 27.399832369337414 27.16035999421338 22.89854492203361 62-63 22.473293653101912 27.51983193188437 27.126743816237063 22.880130598776656 64-65 22.44429587492965 27.474267885943938 26.908369122218573 23.173067116907838 66-67 22.35840393919718 27.729813257546997 26.752681738568118 23.1591010646877 68-69 22.28778702912228 27.3986816613741 27.094612383438538 23.218918926065083 70-71 22.391805894244456 27.437804631148904 26.95878017604772 23.211609298558923 72-73 22.34442860520791 27.410201727408502 26.927319056867894 23.31805061051569 74-75 22.19929041501241 27.71457051524422 26.82258448937719 23.263554580366186 76-77 22.383788677774323 27.387862358322977 26.966820938982423 23.26152802492027 78-79 22.435277586819062 27.294387828333573 27.13340333753862 23.13693124730874 80-81 22.314888994398068 27.738803593909527 26.938721704685854 23.00758570700655 82-83 22.340744982339935 27.6017856852826 26.97425003748821 23.08321929488925 84-85 22.329786848291825 27.366039862451576 26.982055551190857 23.32211773806574 86-87 22.42899895409224 27.10196675229588 27.226727796934497 23.242306496677383 88-89 22.40041943371161 27.150400452674134 27.172044501372866 23.27713561224139 90-91 22.351177919454038 27.494356011145584 27.103648828879397 23.05081724052099 92-93 22.434079071120195 27.03757143558616 27.199573936067743 23.3287755572259 94-95 22.272605373391404 26.92149923134743 27.271000079320412 23.53489531594076 96-97 22.351240579366372 26.96583548409394 27.155031911080325 23.527892025459366 98-99 22.378840049375032 26.9580795711564 27.039439590374588 23.62364078909398 100-101 22.100520988552454 26.90938168445537 27.401663552369115 23.588433774623063 102-103 22.169778359629337 26.730971672382147 27.36017211793674 23.739077850051764 104-105 22.117447891190483 26.923058037257192 27.355278907674403 23.604215163877925 106-107 22.134523964666354 26.944606557160316 27.279320524225305 23.64154895394803 108-109 22.250009820718997 26.90514394152291 27.005201959308984 23.839644278449114 110-111 21.999515009035033 27.281043954383655 27.221515468341117 23.497925568240195 112-113 21.97617031306304 27.10649552362383 27.43768163677254 23.479652526540594 114-115 21.899972350522848 27.165724620272957 27.20549907306261 23.728803956141583 116-117 21.77994555542419 27.269842864659488 27.264592555212293 23.685619024704028 118-119 21.7107714399078 27.36633476297542 27.005044684187656 23.91784911292913 120-121 21.791780451401625 27.388043942660428 27.121571394734843 23.698604211203104 122-123 21.832346009829294 27.50539870733208 27.125100051257974 23.537155231580652 124-125 21.8235351887106 27.511067295198384 27.08586408001692 23.579533436074094 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 851.0 1 722.5 2 542.0 3 423.0 4 317.0 5 256.0 6 252.5 7 260.0 8 261.5 9 274.0 10 242.5 11 210.0 12 214.5 13 218.0 14 238.5 15 287.5 16 331.0 17 381.0 18 504.0 19 620.0 20 814.5 21 1108.0 22 1541.0 23 2141.5 24 2969.0 25 4204.5 26 5577.5 27 7183.5 28 8992.5 29 11232.0 30 14183.5 31 16896.0 32 20025.0 33 23026.0 34 25879.5 35 28745.0 36 32291.5 37 35814.0 38 38018.5 39 40214.5 40 41644.0 41 44043.5 42 47027.5 43 49109.5 44 50928.0 45 52894.5 46 52462.5 47 51227.5 48 51495.0 49 50295.5 50 48807.5 51 46919.5 52 45291.5 53 44642.0 54 43893.5 55 41522.5 56 37133.5 57 33390.0 58 30655.0 59 26981.0 60 22326.5 61 18252.0 62 14889.5 63 11635.5 64 9192.0 65 7326.0 66 5496.5 67 4651.5 68 3474.0 69 2067.0 70 1514.5 71 1234.0 72 972.0 73 713.0 74 554.5 75 467.5 76 319.0 77 160.0 78 77.5 79 49.0 80 42.0 81 32.5 82 23.0 83 14.0 84 11.5 85 13.0 86 8.0 87 6.5 88 5.5 89 3.0 90 4.5 91 4.5 92 3.0 93 3.0 94 4.5 95 3.5 96 0.5 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04207717724840945 2 0.007931963395122069 3 0.0022662752557491626 4 0.005287975596748046 5 0.023946975202416152 6 0.02968820585031403 7 0.0028706153239489393 8 7.554250852497208E-5 9 0.0 10-11 7.554250852497208E-5 12-13 9.442813565621512E-4 14-15 3.7771254262486045E-4 16-17 1.1331376278745812E-4 18-19 7.554250852497208E-5 20-21 7.554250852497208E-5 22-23 0.0 24-25 0.0 26-27 3.777125426248604E-5 28-29 1.1331376278745812E-4 30-31 1.5108501704994416E-4 32-33 1.1331376278745812E-4 34-35 6.043400681997766E-4 36-37 3.777125426248604E-5 38-39 1.5108501704994416E-4 40-41 4.532550511498325E-4 42-43 6.043400681997766E-4 44-45 0.0011709088821370672 46-47 7.554250852497208E-5 48-49 0.0 50-51 0.0 52-53 0.0010198238650871232 54-55 0.0029083865782114256 56-57 2.2662752557491624E-4 58-59 6.798825767247488E-4 60-61 0.0010953663736120952 62-63 6.798825767247488E-4 64-65 0.001737477696074358 66-67 0.002757301561161481 68-69 0.003512726646411202 70-71 0.0019641052216492743 72-73 0.0030972428495238554 74-75 0.00200187647591176 76-77 0.0012464513906620396 78-79 0.0021151902386992187 80-81 0.0013975364077119835 82-83 6.421113224622627E-4 84-85 4.532550511498325E-4 86-87 0.0020774189844367324 88-89 0.005174661833960588 90-91 3.399412883623744E-4 92-93 9.820526108246371E-4 94-95 9.820526108246371E-4 96-97 1.1331376278745812E-4 98-99 9.06510102299665E-4 100-101 0.0018507914588618162 102-103 0.0010953663736120952 104-105 0.0010198238650871232 106-107 0.006836597021509973 108-109 0.0019641052216492743 110-111 0.001661935187549386 112-113 0.0023418177642741344 114-115 0.003323870375098772 116-117 0.00200187647591176 118-119 0.04404128247005873 120-121 0.012804455194982767 122-123 0.004645864274285783 124-125 0.0028706153239489393 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1323758.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.88360707295263 #Duplication Level Percentage of deduplicated Percentage of total 1 86.01358107957738 62.6898004634147 2 9.01289785853327 13.1378501221999 3 2.2865330438213265 4.999523277755877 4 0.9487436892998944 2.765914490555078 5 0.4745787323606598 1.7294504927277137 6 0.2731901637652388 1.1946650731236745 7 0.17850619447152896 0.9107122736565689 8 0.124437493764375 0.725556272053256 9 0.08811889591566602 0.5780180687057831 >10 0.5427227535576151 7.385517674351755 >50 0.0436826878501091 2.224227978455492 >100 0.012813266678960293 1.4925053912162116 >500 0.0 0.0 >1k 1.9414040394057533E-4 0.16625842178402303 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 3.777125426248605E-5 0.0 24-25 0.0 0.0 0.0 1.510850170499442E-4 0.0 26-27 0.0 0.0 0.0 6.421113224622627E-4 0.0 28-29 0.0 0.0 0.0 0.003021700340998883 0.0 30-31 0.0 0.0 0.0 0.01012269614234626 0.0 32-33 0.0 0.0 0.0 0.02768632937440227 0.0 34-35 0.0 0.0 0.0 0.05333301101863029 0.0 36-37 0.0 0.0 0.0 0.09529687450425228 0.0 38-39 0.0 0.0 0.0 0.15913029420785368 0.0 40-41 0.0 0.0 0.0 0.22560770170982913 0.0 42-43 0.0 0.0 0.0 0.2929538480598417 0.0 44-45 0.0 0.0 0.0 0.3753707248605863 0.0 46-47 0.0 0.0 0.0 0.48139463557538464 0.0 48-49 0.0 0.0 0.0 0.5899114490715069 0.0 50-51 0.0 0.0 0.0 0.7113082602711371 0.0 52-53 0.0 0.0 0.0 0.8289657172987812 0.0 54-55 0.0 0.0 0.0 0.951420123617761 0.0 56-57 0.0 0.0 0.0 1.10273176819328 0.0 58-59 0.0 0.0 0.0 1.2753086289185789 0.0 60-61 0.0 0.0 0.0 1.4680175681657825 0.0 62-63 0.0 0.0 0.0 1.655816244358863 0.0 64-65 0.0 0.0 0.0 1.846032280824743 0.0 66-67 0.0 0.0 0.0 2.061706142663538 0.0 68-69 0.0 0.0 0.0 2.314660232459407 0.0 70-71 0.0 0.0 0.0 2.589068394676368 0.0 72-73 0.0 0.0 0.0 2.862041249231355 0.0 74-75 0.0 0.0 0.0 3.1336543386328923 0.0 76-77 0.0 0.0 0.0 3.425059565267972 0.0 78-79 0.0 0.0 0.0 3.751780914638476 0.0 80-81 0.0 0.0 0.0 4.120654983765915 0.0 82-83 0.0 0.0 0.0 4.498934095204713 0.0 84-85 0.0 0.0 0.0 4.875966755252849 0.0 86-87 0.0 0.0 0.0 5.258174077134944 0.0 88-89 0.0 0.0 0.0 5.671807082563429 0.0 90-91 0.0 0.0 0.0 6.136544595009058 0.0 92-93 0.0 0.0 0.0 6.61926122448363 0.0 94-95 0.0 0.0 0.0 7.102619965280663 0.0 96-97 0.0 0.0 0.0 7.580917358006523 0.0 98-99 0.0 0.0 0.0 8.092189055703535 0.0 100-101 0.0 0.0 0.0 8.650372651194553 0.0 102-103 0.0 0.0 0.0 9.238357766298673 0.0 104-105 0.0 0.0 0.0 9.829704523032156 0.0 106-107 0.0 0.0 0.0 10.395857853172558 0.0 108-109 0.0 0.0 0.0 10.999744666321185 0.0 110-111 0.0 0.0 0.0 11.630184671216341 0.0 112-113 0.0 0.0 0.0 12.306705606311727 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1460 0.0 50.55448 1 GTATCAA 2105 0.0 45.243767 1 TCAACGC 2320 0.0 38.995422 4 ATCAACG 2370 0.0 38.42387 3 GTACATG 6080 0.0 37.10449 1 CAACGCA 2500 0.0 36.187756 5 AACGCAG 2560 0.0 35.334263 6 TACATGG 6495 0.0 34.27287 2 TATCAAC 2765 0.0 33.795784 2 ACATGGG 6435 0.0 33.575005 3 CATGGGG 3100 0.0 28.991673 4 ACGCAGA 3145 0.0 28.942226 7 CGCAGAG 3205 0.0 27.657911 8 CATGGGA 3870 0.0 27.22202 4 CCGATAG 90 0.009514396 26.453035 3 ATGGGAT 1845 0.0 22.904455 5 ATGGGGA 1820 0.0 22.892048 5 CTACACG 470 0.0 22.794636 4 GAGTACA 4920 0.0 22.744877 1 GCAGAGT 4025 0.0 21.875448 9 >>END_MODULE