FastQCFastQC Report
Thu 26 May 2016
SRR1364395_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364395_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1323758
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37850.28592839476701937No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37730.28502188466471967No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20270.15312466478011844No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28250.074.852261
GTATCAA47450.050.96651
ATCAACG57900.040.7191053
TCAACGC58350.040.4050754
CAACGCA59200.039.8249325
AACGCAG60200.039.1471146
TATCAAC61200.038.718032
GTACCGT957.463188E-637.5862246
GTACATG58250.034.1543161
GTGGTAT12900.033.707631
ACGCAGA70950.033.2082067
CGCAGAG71400.032.9989138
ACATGGG61600.031.8944283
TACATGG63150.031.5829752
TGGTATC12750.030.8187712
GCAGAGT78650.029.5031599
CAGAGTA77600.027.71719210-11
GAGTACT50850.027.49663212-13
CATGGGG28100.027.1197224
CGCGGAA2900.026.67153582-83
Produced by FastQC (version 0.11.5)