##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364395_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1323758 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.27847461545086 33.0 31.0 34.0 30.0 34.0 2 32.28604397480506 33.0 31.0 34.0 30.0 34.0 3 32.3924554185886 34.0 31.0 34.0 30.0 34.0 4 36.01516062603588 37.0 35.0 37.0 35.0 37.0 5 35.94946659434731 37.0 35.0 37.0 35.0 37.0 6 36.014164220348434 37.0 35.0 37.0 35.0 37.0 7 35.97405870257253 37.0 35.0 37.0 35.0 37.0 8 35.98020635191629 37.0 35.0 37.0 35.0 37.0 9 37.71761908143331 39.0 38.0 39.0 35.0 39.0 10-11 37.68237056924302 39.0 37.0 39.0 35.0 39.0 12-13 37.65132184281417 39.0 37.0 39.0 35.0 39.0 14-15 39.04145508469071 41.0 38.0 41.0 35.5 41.0 16-17 39.051660877592425 41.0 38.0 41.0 35.5 41.0 18-19 39.11524085217993 41.0 38.5 41.0 36.0 41.0 20-21 39.12917277931464 41.0 39.0 41.0 35.0 41.0 22-23 39.09072579731341 41.0 39.0 41.0 35.0 41.0 24-25 39.057914286448124 41.0 39.0 41.0 35.0 41.0 26-27 38.962100323473024 41.0 38.5 41.0 35.0 41.0 28-29 38.86963931473879 40.5 38.0 41.0 35.0 41.0 30-31 38.75294238070705 40.0 38.0 41.0 35.0 41.0 32-33 38.61782402825894 40.0 38.0 41.0 34.0 41.0 34-35 38.52241988339259 40.0 38.0 41.0 34.0 41.0 36-37 38.404191702713035 40.0 38.0 41.0 34.0 41.0 38-39 38.317920647127345 40.0 38.0 41.0 34.0 41.0 40-41 38.201326828619734 40.0 38.0 41.0 33.5 41.0 42-43 38.051665410142945 40.0 38.0 41.0 33.0 41.0 44-45 37.86860173838421 40.0 37.5 41.0 33.0 41.0 46-47 37.61340705778548 40.0 37.0 41.0 33.0 41.0 48-49 37.38078636729674 40.0 37.0 41.0 32.0 41.0 50-51 37.21989442179009 40.0 37.0 41.0 32.0 41.0 52-53 37.07694684375845 40.0 36.5 41.0 31.0 41.0 54-55 36.89941741617426 40.0 36.0 41.0 31.0 41.0 56-57 36.711090697846586 39.5 36.0 41.0 31.0 41.0 58-59 36.50729211834791 39.0 35.0 41.0 31.0 41.0 60-61 36.26268245404371 39.0 35.0 41.0 30.0 41.0 62-63 36.032365432352435 38.5 35.0 41.0 30.0 41.0 64-65 35.75635388039204 38.0 35.0 40.0 30.0 41.0 66-67 35.43495638930983 37.0 35.0 40.0 29.0 41.0 68-69 35.10545432020052 37.0 34.5 39.5 29.0 41.0 70-71 34.85478123644957 36.0 34.5 39.0 29.0 41.0 72-73 34.65279907656837 36.0 34.0 39.0 29.0 41.0 74-75 34.31509649044614 35.5 34.0 38.5 29.0 40.5 76-77 33.978510422599896 35.0 34.0 37.0 29.0 39.0 78-79 33.652171696035076 35.0 34.0 37.0 29.0 39.0 80-81 33.3490996843834 35.0 34.0 36.5 29.0 39.0 82-83 33.07028701620689 35.0 34.0 36.0 29.0 37.0 84-85 32.83156437959204 35.0 34.0 36.0 28.0 37.0 86-87 32.63568265498679 35.0 34.0 35.0 28.0 37.0 88-89 32.4727975203927 35.0 34.0 35.0 27.0 36.0 90-91 32.33280252130676 35.0 33.0 35.0 27.0 36.0 92-93 32.20492680686349 35.0 33.0 35.0 27.0 36.0 94-95 32.12233958170602 35.0 33.0 35.0 27.0 36.0 96-97 32.037357281315764 35.0 33.0 35.0 27.0 35.0 98-99 31.969134464154322 35.0 33.0 35.0 26.0 35.0 100-101 31.907532947865093 35.0 33.0 35.0 26.0 35.0 102-103 30.93520530187542 34.0 31.5 35.0 24.5 35.0 104-105 31.656149386821458 35.0 33.0 35.0 25.0 35.0 106-107 31.94718936542782 35.0 33.0 35.0 27.0 35.0 108-109 31.96186991882202 35.0 33.5 35.0 27.0 35.0 110-111 31.934942791658294 35.0 34.0 35.0 27.0 35.0 112-113 31.870936001897626 35.0 33.5 35.0 26.5 35.0 114-115 31.801396101100053 35.0 33.0 35.0 25.0 35.0 116-117 31.743977751220392 35.0 33.0 35.0 25.0 35.0 118-119 31.648578138904544 35.0 33.0 35.0 25.0 35.0 120-121 31.52452298682992 35.0 33.0 35.0 25.0 35.0 122-123 31.37842301991754 35.0 33.0 35.0 25.0 35.0 124-125 30.696075113427078 34.5 32.0 35.0 21.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13352575332497452 1101 2 0.14155744690946648 1101 3 0.20977643091975295 1101 4 0.07620403984244462 1101 5 0.21791641184096022 1101 6 0.20418967256501475 1101 7 0.3085898712980253 1101 8 0.24393323209056916 1101 9 0.16214562962015577 1101 10-11 0.2690159978987836 1101 12-13 0.28833492369076197 1101 14-15 0.3376521518363873 1101 16-17 0.32314499759562665 1101 18-19 0.31517011229306746 1101 20-21 0.3480621990293713 1101 22-23 0.3005006811317372 1101 24-25 0.3225655953911257 1101 26-27 0.3228152747534452 1101 28-29 0.22047359134373323 1101 30-31 0.3227378706056143 1101 32-33 0.26042827131037427 1101 34-35 0.24809989938496813 1101 36-37 0.3679317328826457 1101 38-39 0.32669164079285196 1101 40-41 0.2855878404881338 1101 42-43 0.2479317938908281 1101 44-45 0.13396221401946207 1101 46-47 0.0950121002857145 1101 48-49 0.13584143005531502 1101 50-51 0.24003606486051865 1101 52-53 0.20666545212510812 1101 54-55 0.19076955441959598 1101 56-57 0.30494791214562156 1101 58-59 0.12004567283972989 1101 60-61 0.17774042714059846 1101 62-63 0.07063364882659329 1101 64-65 0.105562292432694 1101 66-67 0.019552227778952158 1101 68-69 0.07980386609345658 1101 70-71 0.1197091531721739 1101 72-73 0.1057061451527943 1101 74-75 0.04424660304270134 1101 76-77 -0.11247478504837005 1101 78-79 -0.3144120249323379 1101 80-81 -0.12362816510695751 1101 82-83 -0.16061642844439206 1101 84-85 -0.2522420499159921 1101 86-87 -0.21172326908254036 1101 88-89 -0.22534158619959044 1101 90-91 -0.6206805992565165 1101 92-93 -0.5228727644179756 1101 94-95 -0.2954838757111311 1101 96-97 -0.23293991249823165 1101 98-99 -0.14859953844576523 1101 100-101 -0.3248624366694095 1101 102-103 -0.19732992900512158 1101 104-105 -0.15435060769619469 1101 106-107 -0.18489394077608878 1101 108-109 -0.1222312776726433 1101 110-111 -0.17246136828753933 1101 112-113 -0.1594283772899736 1101 114-115 -0.240516977767804 1101 116-117 -0.14974747107943642 1101 118-119 -0.1430245843074438 1101 120-121 -0.15240199307280378 1101 122-123 -0.11168204664982895 1101 124-125 -0.058567430433821954 1102 1 0.12720148714839752 1102 2 0.10534787976142468 1102 3 0.10639928873862203 1102 4 0.12227953926056045 1102 5 0.11657876090126962 1102 6 0.06310188887819379 1102 7 0.1913877682532359 1102 8 0.16648126179291722 1102 9 0.20062909331621626 1102 10-11 0.12911344667764268 1102 12-13 0.14838904050274238 1102 14-15 0.2077664381795259 1102 16-17 0.27095804010497915 1102 18-19 0.2298497446178871 1102 20-21 0.20352367759862489 1102 22-23 0.1811160225738888 1102 24-25 0.19944475835508513 1102 26-27 0.2366784777974189 1102 28-29 0.31806887352425406 1102 30-31 0.2875718472696249 1102 32-33 0.29023832597743393 1102 34-35 0.2023282739822534 1102 36-37 0.30799124211717555 1102 38-39 0.29361421826077816 1102 40-41 0.26562707311339295 1102 42-43 0.3683437639683831 1102 44-45 0.33893548576179455 1102 46-47 0.18432396113919935 1102 48-49 0.2675007279949213 1102 50-51 0.16052396905146793 1102 52-53 0.20510495107001248 1102 54-55 0.31121770325045617 1102 56-57 0.21883656412252606 1102 58-59 0.27218283295892576 1102 60-61 0.23455633918909058 1102 62-63 0.09991670423923438 1102 64-65 0.14707926893439094 1102 66-67 0.05832071952386286 1102 68-69 0.08549892459849673 1102 70-71 0.07336045601997654 1102 72-73 0.22779072915215437 1102 74-75 0.15437610372088528 1102 76-77 0.28090230210803924 1102 78-79 0.2561943199681096 1102 80-81 0.21800945309301767 1102 82-83 0.16670444545820118 1102 84-85 0.1870865934770478 1102 86-87 0.12822949219545876 1102 88-89 0.027276214847532287 1102 90-91 0.07743672995363227 1102 92-93 0.11572790193137905 1102 94-95 0.020977732166208796 1102 96-97 0.01852787190391325 1102 98-99 0.06564732445739452 1102 100-101 0.06713586856921694 1102 102-103 0.05313200351805847 1102 104-105 -0.048472867121244434 1102 106-107 -0.06809984602902475 1102 108-109 -0.01249259006723591 1102 110-111 -0.0459207449124861 1102 112-113 -0.08721985010913613 1102 114-115 -0.13681037507654636 1102 116-117 -0.12635646266890888 1102 118-119 -0.20296658825271763 1102 120-121 -0.28352548780281595 1102 122-123 -0.37165954153940817 1102 124-125 -0.4080057656058962 1103 1 0.1367171362575803 1103 2 0.1269550080748587 1103 3 0.13752178724082142 1103 4 0.1056795903587826 1103 5 -0.009637750518287191 1103 6 -0.04601819528883766 1103 7 -0.009193390337571827 1103 8 0.1133472003657161 1103 9 0.12825761147895776 1103 10-11 0.1892011336798376 1103 12-13 0.1581982533558417 1103 14-15 0.26122014241007463 1103 16-17 0.24882695977924385 1103 18-19 0.26216881172949513 1103 20-21 0.1852644311077185 1103 22-23 0.2601295758750197 1103 24-25 0.23818792062095184 1103 26-27 0.24146279979208174 1103 28-29 0.22614688310349607 1103 30-31 0.29389009212382433 1103 32-33 0.23528737692280544 1103 34-35 0.2542275547022683 1103 36-37 0.2788556332394734 1103 38-39 0.27834430139022714 1103 40-41 0.18556366334470908 1103 42-43 0.22190384844780198 1103 44-45 0.3320716725626056 1103 46-47 0.2993245245584575 1103 48-49 0.2681009647425725 1103 50-51 0.09902912852143686 1103 52-53 0.21650693899164253 1103 54-55 0.2882470724551851 1103 56-57 0.27017256505077114 1103 58-59 0.13744348967147602 1103 60-61 0.25342572410261965 1103 62-63 0.23820014986593208 1103 64-65 0.25408192871952906 1103 66-67 -0.011939474180785226 1103 68-69 -0.02743149145036483 1103 70-71 0.12488500715334538 1103 72-73 0.19980692215616358 1103 74-75 0.234039402144532 1103 76-77 0.16148726517339185 1103 78-79 0.20086403913987283 1103 80-81 0.1821457258013197 1103 82-83 0.2129187701416626 1103 84-85 0.18805235543743493 1103 86-87 0.28025297617929823 1103 88-89 0.21225498342502647 1103 90-91 0.233159953872061 1103 92-93 0.14361827058608156 1103 94-95 0.13353397646925913 1103 96-97 0.07496295652278917 1103 98-99 0.1301770980967305 1103 100-101 0.16226920926605004 1103 102-103 0.2236099782880352 1103 104-105 0.1834655015066815 1103 106-107 0.16995427122688866 1103 108-109 0.13369462355122153 1103 110-111 0.09914743997381947 1103 112-113 0.1188347033783188 1103 114-115 0.10757941955992933 1103 116-117 0.04659316541859937 1103 118-119 0.027220299586687702 1103 120-121 0.010645605330946495 1103 122-123 -0.009215144325491309 1103 124-125 -0.18474349561762438 1104 1 0.10671020310740431 1104 2 0.06996764541494827 1104 3 0.18323198156341647 1104 4 0.1624434075223249 1104 5 0.15828487466178842 1104 6 0.17769954990385628 1104 7 0.17241734675781117 1104 8 0.18194380981768887 1104 9 0.29344153434604436 1104 10-11 0.19113015852470738 1104 12-13 0.2509677444712537 1104 14-15 0.24094823765125994 1104 16-17 0.2643933398325018 1104 18-19 0.23170992790187483 1104 20-21 0.10516008827748635 1104 22-23 0.18962176042208512 1104 24-25 0.29300894383296594 1104 26-27 0.14521944406099863 1104 28-29 0.1735302668695624 1104 30-31 0.19830630360002743 1104 32-33 0.09062752245416306 1104 34-35 0.0971981827679187 1104 36-37 0.19831526869832317 1104 38-39 0.09681914874212794 1104 40-41 0.008315573200796678 1104 42-43 0.05024520566054491 1104 44-45 0.06681415247051348 1104 46-47 0.019893194063179465 1104 48-49 -0.09782141421347745 1104 50-51 -0.11780109353375678 1104 52-53 -0.04523837897212957 1104 54-55 0.010096808844288319 1104 56-57 -0.01119696969381323 1104 58-59 -0.1137468630984344 1104 60-61 -0.13938340044138187 1104 62-63 -0.09940970600661814 1104 64-65 -0.07147455917552747 1104 66-67 -0.015906326811389704 1104 68-69 -0.15529357575833558 1104 70-71 -0.11904335677440514 1104 72-73 -0.03143307499281889 1104 74-75 0.0736914055225597 1104 76-77 0.07329694696124278 1104 78-79 0.19809509878871978 1104 80-81 0.1799877558104157 1104 82-83 0.07685407801888289 1104 84-85 0.104401252720983 1104 86-87 0.12706572496814772 1104 88-89 0.10611649968985404 1104 90-91 0.05702122175300417 1104 92-93 0.05568443573329063 1104 94-95 0.04744007224176272 1104 96-97 0.062408231073376896 1104 98-99 -0.02026497122180615 1104 100-101 -0.07729488781533078 1104 102-103 -0.03992019387439427 1104 104-105 0.015088495806811153 1104 106-107 0.0014879013249675666 1104 108-109 1.603661794291611E-5 1104 110-111 0.0668802134714781 1104 112-113 0.04819999615812165 1104 114-115 -0.10716464784497859 1104 116-117 0.0035563376479856856 1104 118-119 -0.12697317634059502 1104 120-121 -0.16474986501093625 1104 122-123 -0.20583393769817704 1104 124-125 -0.4350429061918675 1105 1 0.03578622004003762 1105 2 0.06811946993995832 1105 3 0.11032923457056398 1105 4 0.03810650526977355 1105 5 0.10395035988511125 1105 6 0.09921290057920373 1105 7 0.14996061913490877 1105 8 0.16499671857574327 1105 9 0.18484751838733615 1105 10-11 0.1650912202387147 1105 12-13 0.15625884456930095 1105 14-15 0.19658679274488122 1105 16-17 0.2076094193578868 1105 18-19 0.26878321643306435 1105 20-21 0.12467321765026185 1105 22-23 0.10838614945006242 1105 24-25 0.18069976122195897 1105 26-27 0.07957320318910632 1105 28-29 0.14989749554837317 1105 30-31 0.2559389243955863 1105 32-33 0.09332265374789728 1105 34-35 0.11654994568708332 1105 36-37 0.10253275229875669 1105 38-39 0.0617633770067485 1105 40-41 0.1095592537415726 1105 42-43 0.03572060493971918 1105 44-45 -0.029536199395579388 1105 46-47 -0.037193522150673175 1105 48-49 -0.08434064884553294 1105 50-51 -0.028298162022416307 1105 52-53 -0.010628452156481671 1105 54-55 -0.10239068681314478 1105 56-57 -0.13720535426982394 1105 58-59 -0.20897574926368634 1105 60-61 -0.23328422200223287 1105 62-63 -0.15062068959185382 1105 64-65 -0.21874593343953563 1105 66-67 -0.23611471507577164 1105 68-69 -0.3192818915810207 1105 70-71 -0.19005456495183637 1105 72-73 -0.14646941297367988 1105 74-75 -0.05360554314372479 1105 76-77 -0.1296690307599775 1105 78-79 -0.0808197759834286 1105 80-81 -0.02153302644430255 1105 82-83 0.09042979414950025 1105 84-85 0.03308338597801708 1105 86-87 -0.08204899997618753 1105 88-89 -0.09643765090454792 1105 90-91 -0.02484265922609552 1105 92-93 0.018761174391407565 1105 94-95 -0.013350720574678121 1105 96-97 -0.05103737011447862 1105 98-99 0.010934079968372146 1105 100-101 -0.017585437149104877 1105 102-103 0.0038035108701031106 1105 104-105 -0.10326077646163512 1105 106-107 -0.059426751147764634 1105 108-109 -0.02774584680644665 1105 110-111 0.0030344234786383595 1105 112-113 0.03241873081584856 1105 114-115 -0.10314244880525436 1105 116-117 -0.10962110863006558 1105 118-119 -0.17962275120540738 1105 120-121 -0.056743746294074526 1105 122-123 0.017426207952805584 1105 124-125 -0.24992804567478544 1106 1 0.11476391041441758 1106 2 0.09477217073747113 1106 3 0.06280701335709438 1106 4 0.0686635968875251 1106 5 0.04380658446348917 1106 6 0.020131666690659245 1106 7 0.0994266322269155 1106 8 0.009496119448954232 1106 9 0.13272336321112732 1106 10-11 0.07409097738665338 1106 12-13 0.15240797624188218 1106 14-15 0.17721702760108116 1106 16-17 0.14406508985340594 1106 18-19 0.18732560887349337 1106 20-21 0.1657741153024972 1106 22-23 0.11434602908948222 1106 24-25 0.2008671115509344 1106 26-27 0.12522888999148307 1106 28-29 0.07674375268793909 1106 30-31 0.09759575567893108 1106 32-33 0.24455295828337142 1106 34-35 0.1568410030039189 1106 36-37 0.1872105881592816 1106 38-39 0.1396698672092569 1106 40-41 0.14694433982400312 1106 42-43 0.20709062318988458 1106 44-45 0.09825575517332652 1106 46-47 0.20439275448208605 1106 48-49 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-0.5200468100672708 2216 36-37 -0.40889143887887514 2216 38-39 -0.3980254678036701 2216 40-41 -0.8091945723925704 2216 42-43 -0.5931896382996769 2216 44-45 -0.535200089645457 2216 46-47 -0.5357771808041889 2216 48-49 -0.3494594763231049 2216 50-51 -0.34965486572553317 2216 52-53 -0.39801055980980493 2216 54-55 -0.329557529969307 2216 56-57 -0.35449869034269454 2216 58-59 -0.2381455110818962 2216 60-61 -0.3553829191410003 2216 62-63 -0.3265796597361543 2216 64-65 -0.26803676178111857 2216 66-67 -0.21883318009133745 2216 68-69 -0.3611880834646115 2216 70-71 -0.47084987401918 2216 72-73 -0.42303055472898166 2216 74-75 -0.5070420306340466 2216 76-77 -0.4771806342855456 2216 78-79 -0.35038792990334855 2216 80-81 -0.34643005667932414 2216 82-83 -0.29284521862623336 2216 84-85 -0.2708042297279576 2216 86-87 -0.4483741726461332 2216 88-89 -0.3753044303137614 2216 90-91 -0.2920581818720649 2216 92-93 -0.24159370119667045 2216 94-95 -0.23591229433411343 2216 96-97 -0.21192150344570848 2216 98-99 -0.2017724259084659 2216 100-101 -0.27025116605070565 2216 102-103 -0.26424599048122843 2216 104-105 -0.23161120556817494 2216 106-107 -0.21770821764373594 2216 108-109 0.0012968425773145498 2216 110-111 -0.0512217128239314 2216 112-113 -0.02448664447457105 2216 114-115 -0.049320107869700536 2216 116-117 -0.031217943409561855 2216 118-119 -0.03974360699505297 2216 120-121 0.011876000509356999 2216 122-123 -0.03899837835055209 2216 124-125 0.012011863604350026 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 192.0 3 16.0 4 41.0 5 104.0 6 235.0 7 488.0 8 867.0 9 1438.0 10 2303.0 11 3228.0 12 4433.0 13 6220.0 14 9632.0 15 8332.0 16 4012.0 17 3513.0 18 3376.0 19 3120.0 20 2970.0 21 3086.0 22 3365.0 23 3762.0 24 4733.0 25 6347.0 26 8586.0 27 11156.0 28 14286.0 29 18413.0 30 23274.0 31 29371.0 32 37377.0 33 48598.0 34 67022.0 35 101825.0 36 202763.0 37 447402.0 38 236739.0 39 1133.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.557998041444016 19.061993737456778 13.546149986701165 23.833858234398047 2 15.849649256132919 20.727731201624465 38.850681166799376 24.571938375443246 3 18.520454645033308 25.31996029485752 30.46818225083437 25.691402809274805 4 12.740697317787692 17.12926380803742 37.16940709706759 32.960631777107295 5 12.961207684993282 38.279992199511106 34.75465497198022 14.00414514351539 6 31.489203518159353 37.27689449375271 17.15023350381139 14.083668484276554 7 27.642665804474838 33.31802338493894 21.328898484466194 17.710412326120032 8 26.269529627016418 35.41682089928824 19.75277958660118 18.560869887094167 9 26.553493916561788 15.383854148567941 19.871456867493908 38.19119506737636 10-11 24.844949001252495 26.567544823147433 27.781248536363893 20.806257639236176 12-13 25.717200575936083 24.18485856176129 28.216902183027408 21.881038679275214 14-15 22.573839024957735 25.46658830390449 26.083204029739576 25.8763686413982 16-17 21.937204534363534 28.946529501615853 26.80603252256077 22.310233441459847 18-19 21.906874217190754 26.977136304369832 29.37213599464555 21.743853483793867 20-21 22.520493761503722 26.272807725590013 29.60688011730254 21.599818395603723 22-23 22.441677406293294 26.269982882067566 29.37428895613851 21.91405075550063 24-25 22.277901247811155 26.47194577860908 29.397895990052564 21.8522569835272 26-27 22.012217531436175 26.82984407595523 29.239167261880706 21.918771130727887 28-29 21.85501778840579 26.503988085413056 29.68369158806139 21.957302538119766 30-31 21.858022982323412 26.321683783988973 29.701119503473638 22.11917373021398 32-33 21.813247517011234 26.547724942068317 29.50234465149395 22.136682889426503 34-35 22.34931424321618 26.542381472725534 29.09896948570224 22.009334798356043 36-37 22.572328174787234 26.17608354397103 28.969078940410558 22.28250934083118 38-39 22.092633245653662 26.356554596837185 29.183166409570326 22.367645747938823 40-41 22.31897546150842 26.214176771114335 29.312215157313616 22.15463261006363 42-43 22.407607734948535 26.159577505858323 29.03933347333878 22.393481285854364 44-45 22.187720253792804 26.412136064247445 28.909384426295766 22.490759255663992 46-47 22.50736161745576 26.726863973626596 28.23801631416014 22.527758094757502 48-49 22.555050804359873 26.77792596739048 28.02094346043772 22.646079767811933 50-51 22.594046645988165 26.785749359021814 27.96406140699433 22.656142587995692 52-53 22.83087241021395 26.7551546430692 27.832730755923667 22.58124219079318 54-55 22.449571598434154 26.818232637687554 27.94170082447094 22.790494939407356 56-57 22.41550648892488 27.085099929707564 27.968306836118906 22.53108674524865 58-59 22.52921042155018 26.592531710880472 27.952184577683063 22.926073289886286 60-61 22.37226229124292 26.648536457772664 28.4182714734383 22.560929777546114 62-63 22.246248936739192 26.74574960075784 28.38286907425677 22.62513238824619 64-65 22.13165095130681 26.64467372435143 28.261434491802884 22.96224083253888 66-67 22.159677221969574 26.86189620761499 28.037488725280603 22.940937845134833 68-69 22.019583639910014 26.689470431906738 28.305060290476053 22.98588563770719 70-71 22.150070651211156 26.68855639235766 28.092100019905473 23.069272936525714 72-73 22.143552944633658 26.67252374022242 27.96147022618603 23.22245308895789 74-75 22.004210739425183 26.86892166090781 27.840964889352886 23.285902710314122 76-77 22.163546632803452 26.774627065239315 27.838681872881505 23.223144429075727 78-79 22.134605469657394 26.731217764583015 27.915271490435373 23.218905275324218 80-81 22.068169714184272 27.1563846784337 27.645032502237953 23.13041310514408 82-83 22.06649553770402 26.976985219352784 27.681230255076834 23.275288987866364 84-85 22.04753436806425 26.73842197743092 27.73101276819479 23.483030886310036 86-87 22.17387921357227 26.539858493773032 27.82596970141068 23.46029259124402 88-89 21.99329484694332 26.66552345670432 27.733543442230378 23.607638254121976 90-91 22.022378712725438 26.928071445082864 27.65516808963572 23.394381752555983 92-93 22.046930027996055 26.46922624830218 27.76406261567447 23.7197811080273 94-95 21.877828122662905 26.400331480527406 27.755073057159997 23.966767339649696 96-97 21.942907993757167 26.418499453827664 27.69909605834299 23.93949649407218 98-99 21.939936015350273 26.304980906587698 27.572587072381218 24.18249600568081 100-101 21.603155561666103 26.270851620915604 27.84969004908752 24.27630276833077 102-103 21.670350622999067 26.017255419797277 27.875676672020113 24.436717285183544 104-105 21.586600112937372 26.03519944400063 27.931451925128943 24.446748517933056 106-107 21.659057017974582 26.167811639287542 27.771994579069588 24.401136763668283 108-109 21.679452976835066 26.06465815018208 27.56736278433975 24.688526088643105 110-111 21.498329133661375 26.345522391724195 27.687050342048835 24.469098132565595 112-113 21.446631484002364 26.187301606486983 27.85784864000822 24.508218269502432 114-115 21.286859078472048 26.23474230184067 27.59669818803739 24.881700431649893 116-117 21.08995753000171 26.262202003689495 27.721494412120645 24.92634605418815 118-119 20.97479297575539 26.44354179540369 27.472279676496765 25.10938555234416 120-121 21.00450687967704 26.477238217977057 27.657782337201624 24.860472565144278 122-123 20.926942048369387 26.52433370185696 27.58809156257165 24.960632687202004 124-125 20.814084150609155 26.54251250701129 27.55395153568535 25.089451806694203 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 147.0 1 110.5 2 169.5 3 261.5 4 174.0 5 125.5 6 307.0 7 806.0 8 1446.5 9 1810.5 10 1497.0 11 968.0 12 865.5 13 974.5 14 1091.5 15 1180.0 16 1322.5 17 1438.5 18 1528.5 19 1632.0 20 1796.0 21 2076.5 22 2446.5 23 2977.5 24 3789.0 25 4962.0 26 6188.0 27 7748.0 28 9519.0 29 11628.5 30 14410.0 31 16892.5 32 19971.5 33 22974.0 34 25635.0 35 28359.5 36 31600.0 37 34994.5 38 37372.5 39 39557.0 40 40882.5 41 43073.5 42 46026.0 43 48242.0 44 50119.0 45 52125.0 46 51598.0 47 50633.0 48 50891.0 49 49487.5 50 48363.0 51 46521.0 52 44668.0 53 44263.5 54 44053.5 55 41395.5 56 36640.0 57 32804.0 58 29907.0 59 26397.5 60 22147.5 61 18262.0 62 14754.5 63 11387.5 64 8961.5 65 7133.0 66 5258.0 67 4436.0 68 3284.5 69 1905.0 70 1374.0 71 1048.0 72 845.0 73 626.0 74 526.0 75 508.5 76 308.5 77 115.0 78 41.5 79 23.0 80 16.0 81 8.0 82 5.5 83 3.5 84 3.5 85 2.0 86 0.5 87 0.5 88 1.0 89 1.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025231197847340674 2 0.0 3 0.0 4 0.0 5 0.05771447651307868 6 0.01699706441811872 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.1331376278745812E-4 22-23 0.0 24-25 0.0 26-27 3.399412883623744E-4 28-29 1.8885627131243023E-4 30-31 1.1331376278745812E-4 32-33 3.777125426248604E-5 34-35 1.1331376278745812E-4 36-37 0.0 38-39 0.0 40-41 7.554250852497208E-5 42-43 0.0 44-45 7.554250852497208E-5 46-47 0.0 48-49 2.6439877983740233E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 1.8885627131243023E-4 58-59 7.554250852497208E-5 60-61 3.777125426248604E-5 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 1.1331376278745812E-4 72-73 1.8885627131243023E-4 74-75 0.0 76-77 7.554250852497208E-5 78-79 3.777125426248604E-5 80-81 2.2662752557491624E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 2.2662752557491624E-4 100-101 0.0 102-103 0.0 104-105 0.0011709088821370672 106-107 0.0 108-109 8.68738848037179E-4 110-111 0.003286099120836286 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 3.021700340998883E-4 122-123 5.665688139372907E-4 124-125 3.777125426248604E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1323758.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.92281872265846 #Duplication Level Percentage of deduplicated Percentage of total 1 84.86016608117875 58.488018435877734 2 9.544680209965307 13.15692527754369 3 2.561036409652329 5.295415446137866 4 1.0322414517826348 2.8457996183691336 5 0.5188010295779176 1.7878614657363696 6 0.30972511939489217 1.280827695474474 7 0.21957194667565574 1.0593462234115218 8 0.15118147526613884 0.8335882731193728 9 0.11053859294192922 0.6856768262874889 >10 0.6189820511667274 8.159355768631505 >50 0.0521681270211263 2.5117169103912573 >100 0.019892577931972424 2.321695606016727 >500 2.0298548892065375E-4 0.08156363588769507 >1k 8.11941955682615E-4 1.4922088171151897 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3785 0.28592839476701937 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3773 0.28502188466471967 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2027 0.15312466478011844 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 3.777125426248605E-5 0.0 24-25 0.0 0.0 0.0 1.510850170499442E-4 0.0 26-27 0.0 0.0 0.0 7.176538309872348E-4 0.0 28-29 0.0 0.0 0.0 0.003021700340998883 0.0 30-31 0.0 0.0 0.0 0.010160467396608745 0.0 32-33 0.0 0.0 0.0 0.02798849940850216 0.0 34-35 0.0 0.0 0.0 0.05389957983256759 0.0 36-37 0.0 0.0 0.0 0.09605229958950201 0.0 38-39 0.0 0.0 0.0 0.16037674559851572 0.0 40-41 0.0 0.0 0.0 0.22643866930360385 0.0 42-43 0.0 0.0 0.0 0.29348264561951654 0.0 44-45 0.0 0.0 0.0 0.37589952242026115 0.0 46-47 0.0 0.0 0.0 0.4824522306947342 0.0 48-49 0.0 0.0 0.0 0.5918000117846314 0.0 50-51 0.0 0.0 0.0 0.7141411043408237 0.0 52-53 0.0 0.0 0.0 0.8316852476056802 0.0 54-55 0.0 0.0 0.0 0.9550083927726971 0.0 56-57 0.0 0.0 0.0 1.1077175737559282 0.0 58-59 0.0 0.0 0.0 1.2810120883122142 0.0 60-61 0.0 0.0 0.0 1.47436313888188 0.0 62-63 0.0 0.0 0.0 1.6631438676857855 0.0 64-65 0.0 0.0 0.0 1.8548329830679022 0.0 66-67 0.0 0.0 0.0 2.0723954076198217 0.0 68-69 0.0 0.0 0.0 2.3274269164001273 0.0 70-71 0.0 0.0 0.0 2.602854902482176 0.0 72-73 0.0 0.0 0.0 2.8767720383937245 0.0 74-75 0.0 0.0 0.0 3.1492538666432988 0.0 76-77 0.0 0.0 0.0 3.442056629686091 0.0 78-79 0.0 0.0 0.0 3.7700622017015197 0.0 80-81 0.0 0.0 0.0 4.141013689813395 0.0 82-83 0.0 0.0 0.0 4.521747932779254 0.0 84-85 0.0 0.0 0.0 4.899951501709527 0.0 86-87 0.0 0.0 0.0 5.284160700067535 0.0 88-89 0.0 0.0 0.0 5.701570830922268 0.0 90-91 0.0 0.0 0.0 6.17000992628562 0.0 92-93 0.0 0.0 0.0 6.655559399829878 0.0 94-95 0.0 0.0 0.0 7.140693389577249 0.0 96-97 0.0 0.0 0.0 7.620879345016234 0.0 98-99 0.0 0.0 0.0 8.134115147934896 0.0 100-101 0.0 0.0 0.0 8.694602789935924 0.0 102-103 0.0 0.0 0.0 9.284514239007432 0.0 104-105 0.0 0.0 0.0 9.879260408624537 0.0 106-107 0.0 0.0 0.0 10.446018078833141 0.0 108-109 0.0 0.0 0.0 11.05285104981424 0.0 110-111 0.0 0.0 0.0 11.687672520203844 0.0 112-113 0.0 0.0 0.0 12.368310522013843 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2825 0.0 74.85226 1 GTATCAA 4745 0.0 50.9665 1 ATCAACG 5790 0.0 40.719105 3 TCAACGC 5835 0.0 40.405075 4 CAACGCA 5920 0.0 39.824932 5 AACGCAG 6020 0.0 39.147114 6 TATCAAC 6120 0.0 38.71803 2 GTACCGT 95 7.463188E-6 37.586224 6 GTACATG 5825 0.0 34.154316 1 GTGGTAT 1290 0.0 33.70763 1 ACGCAGA 7095 0.0 33.208206 7 CGCAGAG 7140 0.0 32.998913 8 ACATGGG 6160 0.0 31.894428 3 TACATGG 6315 0.0 31.582975 2 TGGTATC 1275 0.0 30.818771 2 GCAGAGT 7865 0.0 29.503159 9 CAGAGTA 7760 0.0 27.717192 10-11 GAGTACT 5085 0.0 27.496632 12-13 CATGGGG 2810 0.0 27.119722 4 CGCGGAA 290 0.0 26.671535 82-83 >>END_MODULE