FastQCFastQC Report
Thu 26 May 2016
SRR1364394_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364394_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1405810
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15900.054.6699451
GTATCAA24000.046.389881
ATCAACG26750.040.492123
TCAACGC26900.040.2663234
CAACGCA27800.038.962745
AACGCAG29100.037.216846
TATCAAC30000.036.3038522
GTACATG68850.036.0599441
TACATGG71900.033.8545722
ACATGGG71950.033.003883
TAAGGTG8300.031.5498665
GTAAGGT6600.030.6589934
CATGGGG31150.030.1871384
CGCAGAG36450.029.3804768
ACGCAGA37050.029.227927
CATGGGA46500.027.2614884
AGGTAAG6950.026.5460432
GAGTACA52150.024.4316251
ATGGGGA20650.023.9210875
GCAGAGT45450.022.7771439
Produced by FastQC (version 0.11.5)