##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364394_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1405810 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.54252352736145 31.0 31.0 34.0 26.0 34.0 2 30.519376729430007 31.0 31.0 34.0 26.0 34.0 3 30.696308889536994 31.0 31.0 34.0 26.0 34.0 4 34.10573904012633 37.0 35.0 37.0 30.0 37.0 5 34.16262937381296 37.0 35.0 37.0 30.0 37.0 6 34.23657535513334 37.0 35.0 37.0 30.0 37.0 7 34.11681663951743 37.0 35.0 37.0 30.0 37.0 8 34.02627097545187 37.0 35.0 37.0 30.0 37.0 9 35.46468086014468 39.0 37.0 39.0 30.0 39.0 10-11 35.43063998691146 39.0 36.0 39.0 29.5 39.0 12-13 35.45497471208769 39.0 37.0 39.0 29.5 39.0 14-15 36.69516470931349 40.0 37.0 41.0 29.5 41.0 16-17 36.68743571321872 40.0 37.0 41.0 29.5 41.0 18-19 36.67244435592292 40.0 37.0 41.0 29.0 41.0 20-21 36.70935119255091 40.0 37.0 41.0 29.0 41.0 22-23 36.67032635989216 40.0 37.0 41.0 29.0 41.0 24-25 36.68634346035382 40.0 37.0 41.0 29.0 41.0 26-27 36.65982138411307 40.0 37.0 41.0 29.0 41.0 28-29 36.666077563824416 40.0 37.0 41.0 30.0 41.0 30-31 36.58038319545315 40.0 36.5 41.0 29.5 41.0 32-33 36.544535890340796 40.0 36.0 41.0 30.0 41.0 34-35 36.514107525198995 40.0 36.0 41.0 30.0 41.0 36-37 36.43405901224205 40.0 36.0 41.0 29.0 41.0 38-39 36.32199016936855 40.0 36.0 41.0 29.0 41.0 40-41 36.25323336724024 40.0 36.0 41.0 29.0 41.0 42-43 36.088110768880576 40.0 35.5 41.0 28.0 41.0 44-45 35.916958906253335 40.0 35.0 41.0 28.0 41.0 46-47 35.77539852469395 39.5 35.0 41.0 27.5 41.0 48-49 35.60684658666534 39.0 35.0 41.0 27.0 41.0 50-51 35.461553481622694 39.0 35.0 41.0 27.0 41.0 52-53 35.285722466051595 39.0 35.0 41.0 27.0 41.0 54-55 35.089554420583156 39.0 34.0 40.5 26.0 41.0 56-57 34.87695101044949 38.0 34.0 40.0 26.0 41.0 58-59 34.63926917577766 38.0 34.0 40.0 26.0 41.0 60-61 34.37139833974719 38.0 34.0 40.0 26.0 41.0 62-63 34.09099949495308 37.0 33.0 40.0 25.5 41.0 64-65 33.74280094749646 37.0 33.0 40.0 24.5 41.0 66-67 33.42479389106636 36.0 33.0 39.0 23.5 41.0 68-69 33.09912079157212 36.0 32.5 39.0 23.0 41.0 70-71 32.7443203562359 35.0 32.0 38.5 23.0 40.0 72-73 32.39631991520903 35.0 32.0 37.5 22.0 40.0 74-75 32.0437146556078 35.0 32.0 37.0 21.5 39.0 76-77 31.83110840013942 35.0 32.0 37.0 21.5 39.0 78-79 31.702122619699676 35.0 32.0 36.0 21.5 39.0 80-81 31.45214467104374 35.0 32.0 36.0 20.5 37.5 82-83 31.17600956032465 35.0 32.0 35.5 20.0 37.0 84-85 30.943249443381397 35.0 32.0 35.0 20.0 37.0 86-87 30.743221701367894 35.0 31.0 35.0 18.5 36.0 88-89 30.49364209957249 35.0 31.0 35.0 18.0 36.0 90-91 30.3631297970565 35.0 31.0 35.0 17.0 36.0 92-93 30.214310966631338 34.0 31.0 35.0 14.0 35.5 94-95 30.111610032650216 34.0 31.0 35.0 11.5 35.0 96-97 30.00891550067221 34.0 31.0 35.0 9.0 35.0 98-99 29.869849410660045 34.0 31.0 35.0 7.0 35.0 100-101 29.768886264857983 34.0 31.0 35.0 7.0 35.0 102-103 28.679059047808735 33.5 29.0 34.5 3.5 35.0 104-105 29.434592156834853 34.0 30.0 35.0 2.0 35.0 106-107 29.82196207168821 34.5 31.0 35.0 2.0 35.0 108-109 29.868291589901908 35.0 31.0 35.0 2.0 35.0 110-111 29.80355026639446 35.0 31.0 35.0 2.0 35.0 112-113 29.705510702015207 35.0 31.0 35.0 2.0 35.0 114-115 29.588358313001045 35.0 31.0 35.0 2.0 35.0 116-117 29.440918047246782 35.0 31.0 35.0 2.0 35.0 118-119 29.25414387435002 34.5 31.0 35.0 2.0 35.0 120-121 29.061767948727066 34.0 31.0 35.0 2.0 35.0 122-123 28.856438636800135 34.0 30.0 35.0 2.0 35.0 124-125 27.959514799297203 33.5 28.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4598462629084281 1101 2 0.43734212135027306 1101 3 0.4613478925714922 1101 4 0.6534088505479403 1101 5 0.5109089896827328 1101 6 0.4004703388237445 1101 7 0.4944456749035666 1101 8 0.4290986304761546 1101 9 0.6426459468841514 1101 10-11 0.7746071427091579 1101 12-13 0.7705693995744696 1101 14-15 0.7167279416671661 1101 16-17 0.7339750006811059 1101 18-19 0.6199687726807923 1101 20-21 0.8333442859778799 1101 22-23 0.8442622185043547 1101 24-25 0.7609757831245929 1101 26-27 0.9412011554431032 1101 28-29 0.9311995954949381 1101 30-31 0.8744861197603271 1101 32-33 0.8690536674395517 1101 34-35 0.9006629441535523 1101 36-37 0.8810579681993858 1101 38-39 1.0108358852565331 1101 40-41 0.8421831420611312 1101 42-43 0.9466139187071434 1101 44-45 0.8016032839547194 1101 46-47 0.759108284904265 1101 48-49 0.708892710483525 1101 50-51 0.6540325639772746 1101 52-53 0.6812450811939215 1101 54-55 0.7359850736546392 1101 56-57 0.68987684570493 1101 58-59 0.7272437786519248 1101 60-61 0.6440909710013187 1101 62-63 0.7040875501288966 1101 64-65 0.8066486022611201 1101 66-67 0.7977162503197945 1101 68-69 0.7299846039975932 1101 70-71 0.6456994912208316 1101 72-73 0.6236771265308221 1101 74-75 0.7417355779767618 1101 76-77 0.8039409510884923 1101 78-79 0.7370170426201348 1101 80-81 0.739887610197222 1101 82-83 0.7667879532835258 1101 84-85 0.7786945371451743 1101 86-87 0.7455301753661807 1101 88-89 0.735720027691876 1101 90-91 0.6866420329455636 1101 92-93 0.7088794793699797 1101 94-95 0.6500665304638673 1101 96-97 0.6395832155233947 1101 98-99 0.5943399749531082 1101 100-101 0.5602995955553816 1101 102-103 0.24281531572849602 1101 104-105 0.3666445938210998 1101 106-107 0.3947354116013848 1101 108-109 0.41749549058751256 1101 110-111 0.4045222526579195 1101 112-113 0.4507458563463409 1101 114-115 0.21934280540649098 1101 116-117 0.21886374238571804 1101 118-119 0.3189470755917654 1101 120-121 0.22385549265366933 1101 122-123 0.1336327579847989 1101 124-125 0.06316462066043727 1102 1 0.5909001060904195 1102 2 0.6085098552182373 1102 3 0.6624653106225473 1102 4 0.7702325303006816 1102 5 0.6409885211352631 1102 6 0.6128204600983551 1102 7 0.6335358753209945 1102 8 0.5627062128251552 1102 9 0.8475225348271067 1102 10-11 0.8937093219944003 1102 12-13 0.7557452077479923 1102 14-15 0.9038410573844047 1102 16-17 1.0033947114051571 1102 18-19 0.9616906663477636 1102 20-21 1.0223127545802129 1102 22-23 0.9956218428616879 1102 24-25 1.0605544653119665 1102 26-27 1.0170785960802746 1102 28-29 0.8781125223849813 1102 30-31 0.9781451609479745 1102 32-33 0.9405880826810673 1102 34-35 0.8492534840484396 1102 36-37 0.7333312098318885 1102 38-39 0.8006936247710215 1102 40-41 0.7489307422869871 1102 42-43 0.676244356918815 1102 44-45 0.6779013565914696 1102 46-47 0.6667277821118631 1102 48-49 0.7566150110662306 1102 50-51 0.6440409537568286 1102 52-53 0.5858761480675483 1102 54-55 0.5318130443109865 1102 56-57 0.6204827386677962 1102 58-59 0.6470346285343069 1102 60-61 0.6294119429355689 1102 62-63 0.5182899381299038 1102 64-65 0.601353554059898 1102 66-67 0.5630126244262357 1102 68-69 0.5095209715202316 1102 70-71 0.5339316252195516 1102 72-73 0.5412937204632087 1102 74-75 0.5233609320277353 1102 76-77 0.6674248356250061 1102 78-79 0.7478201982745496 1102 80-81 0.640039174879675 1102 82-83 0.7318668103532318 1102 84-85 0.7910727767545325 1102 86-87 0.6999353811443818 1102 88-89 0.712942804914654 1102 90-91 0.6449360272807496 1102 92-93 0.6617323473258239 1102 94-95 0.6168020832406107 1102 96-97 0.6736320042632613 1102 98-99 0.6891195660713763 1102 100-101 0.5189951334271221 1102 102-103 0.30523516132911865 1102 104-105 0.6190912318512609 1102 106-107 0.6291242493083011 1102 108-109 0.7230274871950222 1102 110-111 0.6965467041789353 1102 112-113 0.6919905384377074 1102 114-115 0.5603384230611468 1102 116-117 0.5895421486476486 1102 118-119 0.5636561422687976 1102 120-121 0.28944665206633857 1102 122-123 0.44210906428718744 1102 124-125 0.40636592736288435 1103 1 0.5026206927796331 1103 2 0.4957703691193345 1103 3 0.51971962836258 1103 4 0.5902284591767994 1103 5 0.3600836579317317 1103 6 0.39075541606534614 1103 7 0.3742755224753509 1103 8 0.33605936492637056 1103 9 0.6534243223189122 1103 10-11 0.6934970419637096 1103 12-13 0.6331528189601343 1103 14-15 0.6307757919001489 1103 16-17 0.42931378998903824 1103 18-19 0.6004670028283954 1103 20-21 0.6981676346721954 1103 22-23 0.6724195684008336 1103 24-25 0.723264312734301 1103 26-27 0.7802447672777433 1103 28-29 0.6819072795827097 1103 30-31 0.9158169254670057 1103 32-33 0.8478103011751514 1103 34-35 0.8877294073771012 1103 36-37 0.859311131585784 1103 38-39 0.7474849304609776 1103 40-41 0.8250856999987519 1103 42-43 0.6437199916897924 1103 44-45 0.5628941202806175 1103 46-47 0.6731587038635212 1103 48-49 0.5920901581970952 1103 50-51 0.6520212513067776 1103 52-53 0.5916813022893237 1103 54-55 0.6415638333708458 1103 56-57 0.5947466369702354 1103 58-59 0.686696434458014 1103 60-61 0.4681897333890106 1103 62-63 0.5760031520585187 1103 64-65 0.6736527995598465 1103 66-67 0.6442810926770619 1103 68-69 0.5749080302674443 1103 70-71 0.6029790046013233 1103 72-73 0.6958043915052201 1103 74-75 0.49762438295392286 1103 76-77 0.43985482683406474 1103 78-79 0.5843576403113637 1103 80-81 0.5905855331251573 1103 82-83 0.612481997434795 1103 84-85 0.47589822172613694 1103 86-87 0.44491984600409396 1103 88-89 0.5442446026961143 1103 90-91 0.4321018092608817 1103 92-93 0.36630900636472674 1103 94-95 0.34673848870665225 1103 96-97 0.30134875246424997 1103 98-99 0.22912104241299858 1103 100-101 0.17959381232505223 1103 102-103 -0.04057941016272437 1103 104-105 0.16916605552909658 1103 106-107 0.17841269738245913 1103 108-109 0.19981648889728731 1103 110-111 0.2212230833839932 1103 112-113 0.19614655195741548 1103 114-115 0.18991804596770834 1103 116-117 0.12987792689218836 1103 118-119 0.14802146076713285 1103 120-121 0.16659601533809365 1103 122-123 0.10364801621884823 1103 124-125 0.07331365815869972 1104 1 0.8495111203084704 1104 2 0.7906709287787166 1104 3 0.7956722807802166 1104 4 0.9136547346799517 1104 5 0.8177670938017627 1104 6 0.8905460984408009 1104 7 0.7710054088522114 1104 8 0.8122399195441261 1104 9 1.1714703310401546 1104 10-11 1.1402998197468506 1104 12-13 1.1129911267219939 1104 14-15 1.2182036802100598 1104 16-17 1.2697883590230177 1104 18-19 1.2265726725386799 1104 20-21 1.1665124434986467 1104 22-23 1.1224125911466842 1104 24-25 1.1844801040931756 1104 26-27 1.3304906431417791 1104 28-29 1.270993953798957 1104 30-31 1.220585637530199 1104 32-33 1.278940622778336 1104 34-35 1.1535634336128666 1104 36-37 1.1664630421637554 1104 38-39 1.0341989524267845 1104 40-41 1.133101889800642 1104 42-43 1.076427429957164 1104 44-45 0.996889617540198 1104 46-47 1.0728209661786963 1104 48-49 1.129940315072787 1104 50-51 1.0660235393316881 1104 52-53 0.9370534535922062 1104 54-55 0.962764782791858 1104 56-57 0.9468635744636842 1104 58-59 0.9270094655894354 1104 60-61 0.9181650718501402 1104 62-63 0.9890328044240704 1104 64-65 0.93363397061961 1104 66-67 0.8677438942664679 1104 68-69 0.8418860689580896 1104 70-71 0.8077575555574654 1104 72-73 0.7893436470804289 1104 74-75 0.7270580421282418 1104 76-77 0.8805944672193107 1104 78-79 1.0115980623419922 1104 80-81 1.022880766792433 1104 82-83 0.8409430209497515 1104 84-85 0.876874908694365 1104 86-87 0.882897522649202 1104 88-89 0.9993126936229189 1104 90-91 0.8479699500895208 1104 92-93 0.8949257158916701 1104 94-95 0.7990803059951936 1104 96-97 0.6963227501350673 1104 98-99 0.8059611886974025 1104 100-101 0.6926878997934551 1104 102-103 0.5126062368859614 1104 104-105 0.6582561379893086 1104 106-107 0.7333728820192533 1104 108-109 0.8049408801559785 1104 110-111 0.7134574316666544 1104 112-113 0.7112193501192827 1104 114-115 0.5563573248861751 1104 116-117 0.610248049325314 1104 118-119 0.5553995372699916 1104 120-121 0.5493138391035828 1104 122-123 0.40958228341102654 1104 124-125 0.36171508241884 1105 1 0.437449301276267 1105 2 0.2542983254201481 1105 3 0.2723489707281459 1105 4 0.4451832635900317 1105 5 0.38281131470186835 1105 6 0.4368916518296544 1105 7 0.3749483086801675 1105 8 0.41117547530836873 1105 9 0.4157492962054192 1105 10-11 0.4082995275801693 1105 12-13 0.5590446361900945 1105 14-15 0.6542069014090899 1105 16-17 0.5964761279805586 1105 18-19 0.6090486255636876 1105 20-21 0.7241331141684881 1105 22-23 0.7013856743706057 1105 24-25 0.7068811863374833 1105 26-27 0.7762584307832512 1105 28-29 0.8051983243915188 1105 30-31 0.7059877380613173 1105 32-33 0.7984712901520936 1105 34-35 0.6576872189882081 1105 36-37 0.6246755344989765 1105 38-39 0.5616620065383913 1105 40-41 0.7105205358044913 1105 42-43 0.6153202778273226 1105 44-45 0.6147046036039683 1105 46-47 0.6793279968997794 1105 48-49 0.596894790470941 1105 50-51 0.6639395806941408 1105 52-53 0.6863050833728366 1105 54-55 0.6139401705725405 1105 56-57 0.4206495942880437 1105 58-59 0.4612604846434891 1105 60-61 0.4436621388281239 1105 62-63 0.5217161896538158 1105 64-65 0.43127515433660335 1105 66-67 0.4112110534426776 1105 68-69 0.31697118705541527 1105 70-71 0.4903427028100822 1105 72-73 0.24502699486863833 1105 74-75 0.371163732810885 1105 76-77 0.4518905026525033 1105 78-79 0.5231791567026214 1105 80-81 0.5883359238105434 1105 82-83 0.5516768319387779 1105 84-85 0.5739738654260726 1105 86-87 0.47688251043773633 1105 88-89 0.3791754121111168 1105 90-91 0.4250031324905308 1105 92-93 0.44538860980263095 1105 94-95 0.44321822094400076 1105 96-97 0.38969903688070673 1105 98-99 0.39856083993877434 1105 100-101 0.3649695681143008 1105 102-103 0.2268690653663228 1105 104-105 0.2132466947448357 1105 106-107 0.2667188595408341 1105 108-109 0.32614383349462983 1105 110-111 0.3512717180035061 1105 112-113 0.3842866767389168 1105 114-115 0.3616506362947334 1105 116-117 0.28390247138463565 1105 118-119 0.37154137805510956 1105 120-121 0.37629377474097936 1105 122-123 0.29696890770124895 1105 124-125 0.18334486918012516 1106 1 0.5778876734175284 1106 2 0.6058810957078578 1106 3 0.6070761134520097 1106 4 0.6426456968625729 1106 5 0.5597214870367822 1106 6 0.45608983746224396 1106 7 0.4804333459727488 1106 8 0.4780598224354691 1106 9 0.7370691067049933 1106 10-11 0.8198037334525949 1106 12-13 0.7039386796027003 1106 14-15 0.8259015854753855 1106 16-17 0.8298916758113393 1106 18-19 0.767232844080894 1106 20-21 0.7210306417150179 1106 22-23 0.8169612216248581 1106 24-25 0.7051558700181317 1106 26-27 0.8246432499473855 1106 28-29 0.7216102741450428 1106 30-31 0.8621648009371938 1106 32-33 0.8382997440813043 1106 34-35 0.827298006091219 1106 36-37 0.7946018569866595 1106 38-39 0.7694667937555479 1106 40-41 0.7298898312120343 1106 42-43 0.698153575224687 1106 44-45 0.681107369719598 1106 46-47 0.6200714255345332 1106 48-49 0.5239018146391885 1106 50-51 0.48019699601914567 1106 52-53 0.49887521452063766 1106 54-55 0.5146798478421672 1106 56-57 0.5810698289879426 1106 58-59 0.5677283562268229 1106 60-61 0.5580259912502754 1106 62-63 0.5418975017395411 1106 64-65 0.5866132242066513 1106 66-67 0.5618009974857898 1106 68-69 0.3745736323377855 1106 70-71 0.4720894677445955 1106 72-73 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100-101 -3.809313400144571 2216 102-103 -3.7315635662590836 2216 104-105 -4.061198215543449 2216 106-107 -3.7000925864874823 2216 108-109 -3.4632020727187474 2216 110-111 -3.538263222736255 2216 112-113 -3.672426623347878 2216 114-115 -3.7103514134181594 2216 116-117 -3.615572473572236 2216 118-119 -3.597691720394856 2216 120-121 -3.458132084986083 2216 122-123 -3.6042196070414825 2216 124-125 -3.5352109168455357 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 32441.0 3 6032.0 4 3987.0 5 3230.0 6 3301.0 7 3926.0 8 4217.0 9 4641.0 10 5029.0 11 5698.0 12 5999.0 13 6127.0 14 5846.0 15 5674.0 16 5181.0 17 4985.0 18 4518.0 19 4360.0 20 4381.0 21 4482.0 22 5386.0 23 7355.0 24 11367.0 25 15606.0 26 17116.0 27 17531.0 28 20623.0 29 24666.0 30 29740.0 31 36309.0 32 46143.0 33 61334.0 34 85189.0 35 130593.0 36 248633.0 37 394033.0 38 129962.0 39 169.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.80158614373075 18.40796373959289 12.623748460149633 24.166701656526723 2 15.800305615470858 20.118902629886577 39.04593337924205 25.034858375400514 3 18.650988426271724 24.99078796673709 29.131294592998852 27.22692901399233 4 12.531336165563085 16.424205605083216 36.59324305482442 34.45121517452928 5 13.374181971862251 39.13088023313891 33.92773035345076 13.567207441548074 6 32.67982180580647 36.55883987354787 17.255891956429085 13.505446364216581 7 29.249764542187496 32.7399464493531 20.553404108205314 17.456884900254096 8 26.70476572564267 35.866465501359 19.338260033902188 18.090508739096137 9 26.895526422489525 16.604590947567594 18.986136106586237 37.51374652335664 10-11 24.9550614628303 27.293430330862872 27.158134568778546 20.593373637528284 12-13 25.350825745372447 24.733486248484393 27.176311121749645 22.739376884393515 14-15 22.455587755351733 26.241529770110528 24.432709776455642 26.870172698082094 16-17 22.834981732925122 29.454093304348817 25.265398973401776 22.445525989324288 18-19 23.074649543430155 28.199776783332588 26.47852588794068 22.24704778529658 20-21 23.65653964618263 27.38325235984948 26.434866731635143 22.52534126233275 22-23 23.592519615026212 27.08178914647072 26.37724870359437 22.9484425349087 24-25 23.26672878980801 27.39804810038341 26.377497670382198 22.95772543942638 26-27 23.010202666222796 27.618652320478816 26.17478619072719 23.196358822571202 28-29 23.032297784513325 27.618982244025414 26.306783605306126 23.041936366155134 30-31 23.033107626996273 27.293501464281423 26.355180540172952 23.318210368549355 32-33 22.96617108013962 27.41777154649031 26.235285163204956 23.38077221016511 34-35 23.55980682898931 27.327409782750188 25.992263485372398 23.120519902888105 36-37 23.58286225226898 27.065056490604345 25.97689302031784 23.375188236808842 38-39 23.12318720735236 27.24811185030667 26.182460969940763 23.446239972400203 40-41 23.274637154613554 26.94532680181406 26.404566515044653 23.375469528527738 42-43 23.083513894559832 26.98296061607538 26.259494580317856 23.674030909046937 44-45 23.132585570858886 27.106958772053375 26.44409493851873 23.31636071856901 46-47 23.419860023658977 27.381173402645736 25.931011965352337 23.267954608342954 48-49 23.216295231930346 27.21335031049715 26.00966702470462 23.560687432867883 50-51 23.13947119454265 27.254500963857136 26.252694176311163 23.35333366528905 52-53 23.374534161048857 27.016702980586814 26.26684364812274 23.341919210241592 54-55 22.949987622459403 27.07124626184047 26.395674405237834 23.583091710462295 56-57 22.996038936996797 27.43888841269034 26.312348863768527 23.252723786544337 58-59 23.111699308792637 26.91138361688461 26.21132009438036 23.765596979942398 60-61 22.842700617514478 27.141827731884476 26.595802950212583 23.419668700388463 62-63 22.91503355380061 27.162573392270033 26.62576237620945 23.296630677719907 64-65 22.77431688644265 27.081333416323588 26.415798973884748 23.728550723349013 66-67 22.755649423124666 27.303286542610667 26.215656550926436 23.72540748333823 68-69 22.796919398661725 26.87569646267209 26.651085583680622 23.67629855498556 70-71 22.71605122694924 26.923313582781418 26.533566512908997 23.827068677360348 72-73 22.79561279739474 26.92505640145387 26.332320958506383 23.947009842645013 74-75 22.742838322612126 27.179126762987977 26.27254885362656 23.805486060773333 76-77 22.97108936386642 26.875421246658927 26.50104300228162 23.652446387193034 78-79 22.928220911046797 26.965556807135687 26.585340435922163 23.520881845895353 80-81 22.987551158829515 27.148795324491115 26.447340950146657 23.41631256653271 82-83 22.79284822574966 27.098530735059274 26.456941040898275 23.651679998292792 84-85 22.780372377524756 26.942117485041482 26.619419698827258 23.658090438606504 86-87 22.92132153921119 26.861753397567906 26.619291001113254 23.597634062107648 88-89 22.827380179613648 26.943576672633867 26.51241570717761 23.71662744057487 90-91 22.79600556406985 26.96688837822871 26.647925335332662 23.589180722368777 92-93 22.808703548064813 26.558006877961184 26.82835222360025 23.804937350373756 94-95 22.63283914189663 26.53734747097378 26.92506320253692 23.904750184592665 96-97 22.89024943697779 26.393293242453087 26.79082320035624 23.925634120212887 98-99 22.686435300712194 26.52148675910303 26.559970294409712 24.232107645775063 100-101 22.478225549447423 26.458518284563116 26.965675897155734 24.097580268833728 102-103 22.499212185060752 26.160968909230203 27.107591690633075 24.23222721507597 104-105 22.302903900201947 26.527014174857143 26.86369103975746 24.306390885183443 106-107 22.438982400951556 26.542237645132218 26.69248296757276 24.32629698634347 108-109 22.523018220735196 26.55392329642759 26.582305944315237 24.340752538521976 110-111 22.321474618318916 26.6931524145719 26.876643873985223 24.10872909312396 112-113 22.268132099516745 26.801686046863164 27.037463716594456 23.892718137025632 114-115 22.23450107735394 26.936445009421956 26.779732514605985 24.049321398618112 116-117 22.127134537909065 26.844983046760213 26.96658826408285 24.061294151247868 118-119 21.951180457950077 27.05079438504101 26.6465742701087 24.35145088690021 120-121 21.973383209431592 27.168078917684873 26.815920274128825 24.04261759875471 122-123 21.990655442194775 27.212040935081788 26.843942470076215 23.953361152647222 124-125 22.01754981880031 27.297754005902103 26.73948325098816 23.945212924309427 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1226.0 1 959.5 2 571.5 3 380.5 4 315.0 5 285.5 6 242.5 7 213.5 8 214.5 9 230.0 10 210.5 11 210.5 12 217.5 13 218.0 14 251.0 15 300.5 16 354.0 17 440.5 18 561.5 19 671.5 20 868.0 21 1241.5 22 1716.0 23 2430.0 24 3300.0 25 4296.5 26 5694.0 27 7400.5 28 9242.0 29 11494.0 30 14217.0 31 17075.5 32 20673.0 33 23317.0 34 25377.5 35 27640.5 36 30465.0 37 33827.5 38 36409.0 39 38586.0 40 39369.0 41 40973.0 42 43083.5 43 44437.0 44 46498.5 45 49152.0 46 49995.0 47 50285.5 48 50771.5 49 50325.0 50 50902.5 51 51106.5 52 51856.0 53 53737.0 54 55288.5 55 52739.5 56 47275.5 57 43591.5 58 40464.0 59 35302.0 60 28744.0 61 23844.0 62 19948.0 63 15683.0 64 12057.0 65 9452.0 66 7125.0 67 5672.5 68 4228.0 69 2679.0 70 1842.5 71 1302.5 72 951.0 73 702.5 74 530.5 75 412.5 76 283.5 77 168.5 78 90.0 79 50.5 80 33.0 81 24.5 82 17.0 83 13.5 84 11.0 85 9.5 86 8.5 87 7.0 88 5.0 89 2.0 90 2.5 91 4.0 92 5.0 93 4.0 94 2.0 95 1.5 96 2.0 97 2.5 98 1.5 99 1.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.045596488856957905 2 0.008678270890091834 3 0.002845334718062896 4 0.006117469643835227 5 0.02105547691366543 6 0.0268172797177428 7 0.0027030679821597515 8 7.113336795157241E-5 9 0.0 10-11 7.113336795157241E-5 12-13 0.001387100675055662 14-15 2.8453347180628963E-4 16-17 1.4226673590314481E-4 18-19 7.113336795157241E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 7.113336795157241E-5 28-29 1.0670005192735862E-4 30-31 7.113336795157241E-5 32-33 7.113336795157241E-5 34-35 6.402003115641516E-4 36-37 7.113336795157241E-5 38-39 1.77833419878931E-4 40-41 8.180337314430827E-4 42-43 5.690669436125793E-4 44-45 0.0011381338872251585 46-47 7.113336795157241E-5 48-49 0.0 50-51 0.0 52-53 9.247337833704413E-4 54-55 0.0027030679821597515 56-57 3.5566683975786204E-5 58-59 6.402003115641516E-4 60-61 9.247337833704413E-4 62-63 5.690669436125793E-4 64-65 0.001600500778910379 66-67 0.0033432682937239028 68-69 0.0032365682417965446 70-71 0.0018850342507166686 72-73 0.0028097680340871097 74-75 0.0020628676705956 76-77 8.891670993946551E-4 78-79 0.002240701090474531 80-81 0.0011737005712009446 82-83 3.55666839757862E-4 84-85 3.912335237336482E-4 86-87 0.0017783341987893102 88-89 0.005014902440585854 90-91 3.201001557820758E-4 92-93 0.0011025672032493722 94-95 8.536004154188689E-4 96-97 2.8453347180628963E-4 98-99 9.958671513220136E-4 100-101 0.0024185345103534617 102-103 0.0016360674628861653 104-105 7.824670474672964E-4 106-107 0.007753537106721393 108-109 0.0015293674109588067 110-111 0.001600500778910379 112-113 0.002596367930232393 114-115 0.0036278017655301926 116-117 0.002240701090474531 118-119 0.045169688649248474 120-121 0.01376430669862926 122-123 0.005121602492513213 124-125 0.0028809014020386822 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1405810.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.39819989079432 #Duplication Level Percentage of deduplicated Percentage of total 1 85.99438278802852 63.97827280149229 2 8.987482847804353 13.373050908520673 3 2.4131763204144883 5.386079227737858 4 0.973467733554057 2.8969698811277294 5 0.4698483932525691 1.7477937339786587 6 0.27704171664028054 1.2366843007615436 7 0.17972779019157586 0.9359996842422523 8 0.11753293952024219 0.6995391302543695 9 0.08780584264625531 0.5879336969498161 >10 0.45830465983380697 6.304579079254837 >50 0.030001340311166472 1.497667974256267 >100 0.010876764439022815 1.1901937765428023 >500 3.5086336369878504E-4 0.1652358048809622 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 7.113336795157241E-5 0.0 0.0 0.0 0.0 9 7.113336795157241E-5 0.0 0.0 0.0 0.0 10-11 7.113336795157241E-5 0.0 0.0 0.0 0.0 12-13 7.113336795157241E-5 0.0 0.0 0.0 0.0 14-15 1.4226673590314481E-4 0.0 0.0 0.0 3.5566683975786204E-5 16-17 1.4226673590314481E-4 0.0 0.0 0.0 7.113336795157241E-5 18-19 1.4226673590314481E-4 0.0 0.0 3.5566683975786204E-5 7.113336795157241E-5 20-21 1.4226673590314481E-4 0.0 0.0 7.113336795157241E-5 7.113336795157241E-5 22-23 1.77833419878931E-4 0.0 0.0 1.77833419878931E-4 7.113336795157241E-5 24-25 2.8453347180628963E-4 0.0 0.0 7.11333679515724E-4 7.113336795157241E-5 26-27 2.8453347180628963E-4 0.0 0.0 9.958671513220136E-4 7.113336795157241E-5 28-29 3.912335237336482E-4 0.0 0.0 0.004019035289263841 7.113336795157241E-5 30-31 4.979335756610068E-4 0.0 0.0 0.012839572915258819 7.113336795157241E-5 32-33 4.979335756610068E-4 0.0 0.0 0.030409514799297203 7.113336795157241E-5 34-35 6.046336275883654E-4 0.0 0.0 0.060427796074860755 7.113336795157241E-5 36-37 7.824670474672964E-4 0.0 0.0 0.11103918737240454 7.113336795157241E-5 38-39 7.824670474672964E-4 0.0 0.0 0.1830261557393958 7.113336795157241E-5 40-41 7.824670474672964E-4 0.0 0.0 0.26255326110925375 7.113336795157241E-5 42-43 8.180337314430827E-4 0.0 0.0 0.34065769912008026 7.113336795157241E-5 44-45 0.001067000519273586 0.0 0.0 0.4412402814036036 7.113336795157241E-5 46-47 0.0012804006231283034 0.0 0.0 0.564941208271388 7.113336795157241E-5 48-49 0.0013515339910798758 0.0 0.0 0.688891101927003 7.113336795157241E-5 50-51 0.0013515339910798758 0.0 0.0 0.8295217703672615 1.4226673590314481E-4 52-53 0.0013515339910798758 0.0 0.0 0.9635014689040482 1.4226673590314481E-4 54-55 0.0013515339910798758 0.0 0.0 1.1050568711276774 1.4226673590314481E-4 56-57 0.0013515339910798758 0.0 0.0 1.2716512188702598 2.134001038547172E-4 58-59 0.0013515339910798758 0.0 0.0 1.4627510118721592 2.134001038547172E-4 60-61 0.0013515339910798758 0.0 0.0 1.6794943840206003 2.134001038547172E-4 62-63 0.0013515339910798758 0.0 0.0 1.888128552222562 2.134001038547172E-4 64-65 0.0013515339910798758 0.0 0.0 2.0986477546752407 2.134001038547172E-4 66-67 0.001422667359031448 0.0 0.0 2.336802270577105 2.134001038547172E-4 68-69 0.001422667359031448 0.0 0.0 2.609100803095724 2.134001038547172E-4 70-71 0.001422667359031448 0.0 0.0 2.9154722188631466 2.134001038547172E-4 72-73 0.001422667359031448 0.0 0.0 3.224119902405019 2.134001038547172E-4 74-75 0.001422667359031448 0.0 0.0 3.517224945049473 2.134001038547172E-4 76-77 0.001422667359031448 0.0 0.0 3.8347642995852924 2.134001038547172E-4 78-79 0.001422667359031448 0.0 0.0 4.210490749105498 2.134001038547172E-4 80-81 0.0015649340949345928 0.0 0.0 4.618902981199451 2.134001038547172E-4 82-83 0.0016360674628861653 0.0 0.0 5.041719720303597 2.8453347180628963E-4 84-85 0.0016716341468619514 0.0 0.0 5.448389184882737 2.8453347180628963E-4 86-87 0.0017072008308377377 3.5566683975786204E-5 0.0 5.867898222377136 2.8453347180628963E-4 88-89 0.0017072008308377377 7.113336795157241E-5 0.0 6.315078140004695 2.8453347180628963E-4 90-91 0.001920600934692455 7.113336795157241E-5 0.0 6.815359116808104 2.8453347180628963E-4 92-93 0.0020628676705956 7.113336795157241E-5 0.0 7.343204273692747 2.8453347180628963E-4 94-95 0.0020628676705956 7.113336795157241E-5 0.0 7.8740725987153315 2.8453347180628963E-4 96-97 0.0020628676705956 7.113336795157241E-5 0.0 8.39224361755856 2.8453347180628963E-4 98-99 0.002347401142401889 7.113336795157241E-5 0.0 8.940183950889523 2.8453347180628963E-4 100-101 0.0024185345103534617 7.113336795157241E-5 0.0 9.55840405175664 2.8453347180628963E-4 102-103 0.0025252345622808203 7.113336795157241E-5 0.0 10.199315697000305 2.8453347180628963E-4 104-105 0.0027742013501113236 7.113336795157241E-5 0.0 10.833114005448817 2.8453347180628963E-4 106-107 0.002845334718062896 7.113336795157241E-5 0.0 11.459514443630361 2.8453347180628963E-4 108-109 0.002845334718062896 7.113336795157241E-5 0.0 12.108962093028218 2.8453347180628963E-4 110-111 0.002845334718062896 7.113336795157241E-5 0.0 12.811688635021802 2.8453347180628963E-4 112-113 0.002845334718062896 7.113336795157241E-5 0.0 13.557024064418378 2.8453347180628963E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1590 0.0 54.669945 1 GTATCAA 2400 0.0 46.38988 1 ATCAACG 2675 0.0 40.49212 3 TCAACGC 2690 0.0 40.266323 4 CAACGCA 2780 0.0 38.96274 5 AACGCAG 2910 0.0 37.21684 6 TATCAAC 3000 0.0 36.303852 2 GTACATG 6885 0.0 36.059944 1 TACATGG 7190 0.0 33.854572 2 ACATGGG 7195 0.0 33.00388 3 TAAGGTG 830 0.0 31.549866 5 GTAAGGT 660 0.0 30.658993 4 CATGGGG 3115 0.0 30.187138 4 CGCAGAG 3645 0.0 29.380476 8 ACGCAGA 3705 0.0 29.22792 7 CATGGGA 4650 0.0 27.261488 4 AGGTAAG 695 0.0 26.546043 2 GAGTACA 5215 0.0 24.431625 1 ATGGGGA 2065 0.0 23.921087 5 GCAGAGT 4545 0.0 22.777143 9 >>END_MODULE