FastQCFastQC Report
Thu 26 May 2016
SRR1364394_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364394_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1405810
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39810.2831819378152097No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37170.2644027286759946No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21160.1505182065855272No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26850.077.4076161
GTATCAA47750.055.000641
ATCAACG61950.041.809333
TCAACGC62850.041.3053674
CAACGCA63750.040.722235
TATCAAC63500.040.6950152
AACGCAG66400.039.0733766
GTGGTAT12400.034.579071
GTACATG68900.033.6227261
ACGCAGA77550.033.4507187
CGCAGAG78950.032.782188
TACATGG71400.032.022722
TGGTATC11600.031.8243062
ACATGGG71650.030.913773
GCAGAGT90250.028.3479739
CATGGGA45550.027.7123094
GAGTACT53000.027.16690812-13
GTACTTT55000.026.07084514-15
CAGAGTA86700.026.00883310-11
AGTACTT55500.024.87114312-13
Produced by FastQC (version 0.11.5)