FastQCFastQC Report
Thu 26 May 2016
SRR1364393_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364393_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1424075
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35840.25167213805452665No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19700.061.9484181
GTATCAA34250.057.0106351
ATCAACG42400.045.198693
TCAACGC43700.043.8541034
CAACGCA43750.043.8024445
AACGCAG45200.042.259676
GTACATG114550.040.8479271
TATCAAC48050.040.0078242
TACATGG116350.039.285352
ACATGGG119250.036.6330833
ACGCAGA56200.033.979857
CATGGGA96900.033.904014
CGCAGAG56500.033.6941348
ATGGGAG34350.033.612095
ATGGGAT36200.031.8943445
CATGGGG28150.030.4452724
GTGGTAT9250.030.2481421
GCAGAGT65100.028.2377599
GAGTACA85150.026.566941
AGTACAT84500.025.6377832
Produced by FastQC (version 0.11.5)