FastQCFastQC Report
Thu 26 May 2016
SRR1364393_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364393_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1424075
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78650.5522883275108404No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67920.47694117233994No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40200.28228850306339204No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17270.12127170268419851No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41350.082.523491
GTATCAA80200.059.4037861
GTGGTAT11700.049.881411
ATCAACG103350.045.796583
TATCAAC104500.045.4065442
TCAACGC104650.045.284574
CAACGCA106400.044.4838035
AACGCAG109000.043.6228076
TGGTATC13600.040.274052
ACGCAGA128700.036.983957
GTACATG105900.036.946041
CGCAGAG130350.036.51588
TACATGG107500.035.8320962
ACATGGG111350.034.005043
GCAGAGT141000.033.63119
CATGGGA89150.032.3221634
GAGTACT83650.030.37132612-13
CAGAGTA137600.029.16522810-11
ATGGGAG30850.028.9475965
ATGGGAT36200.028.2876135
Produced by FastQC (version 0.11.5)