##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364393_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1424075 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.27470814388287 33.0 31.0 34.0 30.0 34.0 2 32.26025314677949 33.0 31.0 34.0 30.0 34.0 3 32.358517634253815 34.0 31.0 34.0 30.0 34.0 4 36.00117549988589 37.0 35.0 37.0 35.0 37.0 5 35.951739901339465 37.0 35.0 37.0 35.0 37.0 6 36.02299036216491 37.0 35.0 37.0 35.0 37.0 7 35.97891262749504 37.0 35.0 37.0 35.0 37.0 8 35.985075224268385 37.0 35.0 37.0 35.0 37.0 9 37.71311202008321 39.0 38.0 39.0 35.0 39.0 10-11 37.68049681372119 39.0 37.0 39.0 35.0 39.0 12-13 37.64812562540597 39.0 37.0 39.0 35.0 39.0 14-15 38.99916752980005 40.5 38.0 41.0 35.0 41.0 16-17 39.01778663342871 40.5 38.0 41.0 35.5 41.0 18-19 39.11840282288503 41.0 38.5 41.0 36.0 41.0 20-21 39.13614311044011 41.0 39.0 41.0 35.0 41.0 22-23 39.08324070010357 41.0 39.0 41.0 35.0 41.0 24-25 39.04259291118797 41.0 39.0 41.0 35.0 41.0 26-27 38.939416112213195 41.0 38.0 41.0 35.0 41.0 28-29 38.826710320734506 40.5 38.0 41.0 35.0 41.0 30-31 38.64802766708215 40.0 38.0 41.0 34.5 41.0 32-33 38.445853975387536 40.0 38.0 41.0 34.0 41.0 34-35 38.3119663641311 40.0 38.0 41.0 34.0 41.0 36-37 38.15972332917859 40.0 38.0 41.0 34.0 41.0 38-39 38.04365851517652 40.0 37.5 41.0 33.0 41.0 40-41 37.893446974351775 40.0 37.0 41.0 33.0 41.0 42-43 37.68254305426329 40.0 37.0 41.0 33.0 41.0 44-45 37.41646437160964 40.0 37.0 41.0 32.5 41.0 46-47 37.01020873198392 40.0 37.0 41.0 31.0 41.0 48-49 36.658613134841914 40.0 36.5 41.0 30.5 41.0 50-51 36.46327616171901 40.0 36.0 41.0 30.0 41.0 52-53 36.30346716289522 40.0 36.0 41.0 30.0 41.0 54-55 36.116884644418306 39.5 35.0 41.0 29.0 41.0 56-57 35.918209012868004 39.0 35.0 41.0 29.0 41.0 58-59 35.69543106929059 39.0 35.0 41.0 28.5 41.0 60-61 35.437488545196004 38.5 35.0 41.0 28.0 41.0 62-63 35.192433685023616 38.0 35.0 40.5 28.0 41.0 64-65 34.889492828678264 37.0 35.0 40.0 28.0 41.0 66-67 34.55658269402946 37.0 34.0 40.0 27.5 41.0 68-69 34.21393957481172 36.0 34.0 39.0 26.5 41.0 70-71 33.9439422081 36.0 34.0 39.0 26.5 41.0 72-73 33.74199006372558 35.5 34.0 39.0 26.5 41.0 74-75 33.41656092551305 35.0 34.0 37.5 26.0 40.0 76-77 33.091300668855226 35.0 34.0 37.0 26.0 39.0 78-79 32.78389094675491 35.0 34.0 37.0 26.0 39.0 80-81 32.49615469690852 35.0 34.0 36.0 26.0 38.5 82-83 32.232294647402696 35.0 33.0 36.0 25.5 37.0 84-85 32.01069817249794 35.0 33.0 35.5 25.0 37.0 86-87 31.830194336674683 35.0 33.0 35.0 25.0 36.5 88-89 31.672730017730807 35.0 33.0 35.0 25.0 36.0 90-91 31.52715236205958 35.0 33.0 35.0 24.0 36.0 92-93 31.401439179818478 35.0 33.0 35.0 24.0 36.0 94-95 31.315955269209837 35.0 33.0 35.0 24.0 35.5 96-97 31.23280866527395 35.0 33.0 35.0 23.5 35.0 98-99 31.16104313326194 35.0 33.0 35.0 23.0 35.0 100-101 31.091729017081263 35.0 33.0 35.0 23.0 35.0 102-103 30.11750329161034 34.0 30.5 35.0 19.5 35.0 104-105 30.821334199392588 35.0 32.0 35.0 20.0 35.0 106-107 31.10314660393589 35.0 33.0 35.0 23.0 35.0 108-109 31.10850271228692 35.0 33.0 35.0 21.5 35.0 110-111 31.061543106929058 35.0 33.0 35.0 20.0 35.0 112-113 30.97874620367607 35.0 33.0 35.0 19.5 35.0 114-115 30.886520723978723 35.0 33.0 35.0 19.0 35.0 116-117 30.81076944683391 35.0 33.0 35.0 18.5 35.0 118-119 30.687879851833646 35.0 33.0 35.0 16.0 35.0 120-121 30.53433913241929 35.0 33.0 35.0 4.5 35.0 122-123 30.370528588733038 35.0 33.0 35.0 2.0 35.0 124-125 29.680868282920493 34.5 31.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13939170626353103 1101 2 0.1159782240713696 1101 3 0.12246257628412849 1101 4 0.10590551955197469 1101 5 0.17848023093087306 1101 6 0.17245121525247953 1101 7 0.20515594108789514 1101 8 0.249317268411275 1101 9 0.18474785431745744 1101 10-11 0.1954636456471377 1101 12-13 0.12530772031477966 1101 14-15 0.2065961817300419 1101 16-17 0.23202691898057282 1101 18-19 0.23777346042432868 1101 20-21 0.23887474088291327 1101 22-23 0.13792094539170563 1101 24-25 0.26342184609500663 1101 26-27 0.31544058239791184 1101 28-29 0.2512771182081508 1101 30-31 0.3024199680657844 1101 32-33 0.24613296622834469 1101 34-35 0.1674564012871329 1101 36-37 0.35884749103856706 1101 38-39 0.2756002405553204 1101 40-41 0.2863931082390536 1101 42-43 0.32880477781388606 1101 44-45 0.25957240131993586 1101 46-47 0.33425310041513256 1101 48-49 0.3124196552930698 1101 50-51 0.36552522849537894 1101 52-53 0.4255100593347123 1101 54-55 0.4575966612280311 1101 56-57 0.5108316482955928 1101 58-59 0.2919919557285837 1101 60-61 0.31171338176579866 1101 62-63 0.2639865336745615 1101 64-65 0.19083668142138777 1101 66-67 0.20220461716765215 1101 68-69 0.20552990396938497 1101 70-71 0.24664996975311482 1101 72-73 0.13310419485608804 1101 74-75 0.1643849503668946 1101 76-77 0.1711373175553348 1101 78-79 0.10791225355842471 1101 80-81 0.10470001155378839 1101 82-83 0.27439780519389245 1101 84-85 0.1440635036618687 1101 86-87 0.16219576508941458 1101 88-89 0.023170601952887893 1101 90-91 -0.4384883213967008 1101 92-93 -0.29525722479063177 1101 94-95 -0.14792449399575958 1101 96-97 -0.041614495736556734 1101 98-99 0.004198501010922229 1101 100-101 -0.1984364887708523 1101 102-103 -0.06181542738099921 1101 104-105 0.011685943564007317 1101 106-107 0.07974926535246141 1101 108-109 0.22275136863571632 1101 110-111 0.11860147234896345 1101 112-113 0.093147742018175 1101 114-115 -0.027384042589066127 1101 116-117 0.06687232210749272 1101 118-119 0.01770983044901442 1101 120-121 0.0675968865845924 1101 122-123 0.13473571374528248 1101 124-125 0.014509978549465785 1102 1 0.06838239950166525 1102 2 0.15648839853186303 1102 3 0.15314023872019789 1102 4 0.11081978408630988 1102 5 0.018148689693340714 1102 6 0.06854363107212436 1102 7 0.22774000312641363 1102 8 0.15414102613323166 1102 9 0.2640946589443445 1102 10-11 0.2587362951350798 1102 12-13 0.2608368103007592 1102 14-15 0.29563187768244603 1102 16-17 0.3204174932454791 1102 18-19 0.31339069556421606 1102 20-21 0.2148756752374794 1102 22-23 0.28031770542120427 1102 24-25 0.28419225751324717 1102 26-27 0.2137130807678247 1102 28-29 0.3304453927003479 1102 30-31 0.37932252645885 1102 32-33 0.2796218925570173 1102 34-35 0.2520734764059185 1102 36-37 0.3782767006772261 1102 38-39 0.31975423380819734 1102 40-41 0.33756038371222274 1102 42-43 0.40156435934112267 1102 44-45 0.4264575448246575 1102 46-47 0.46642795592169506 1102 48-49 0.44964571872468895 1102 50-51 0.3461381913294801 1102 52-53 0.32147197478030876 1102 54-55 0.3083433600020129 1102 56-57 0.3216480441095513 1102 58-59 0.24818447962690726 1102 60-61 0.2579129039516772 1102 62-63 0.1798505761825382 1102 64-65 0.19542528074939014 1102 66-67 0.08623594006891722 1102 68-69 0.00809010107414565 1102 70-71 -0.0473055200133814 1102 72-73 0.11621083943374799 1102 74-75 0.08162580696479438 1102 76-77 0.0640398543935774 1102 78-79 0.08322249652575664 1102 80-81 0.08645936277247301 1102 82-83 0.14509958091272068 1102 84-85 0.24753530562369264 1102 86-87 0.17990187777847666 1102 88-89 0.04843390461511987 1102 90-91 0.007290061790353519 1102 92-93 0.07278135927328222 1102 94-95 0.08092185223977566 1102 96-97 0.14364331981838774 1102 98-99 0.19165725106100595 1102 100-101 0.23157723836903443 1102 102-103 0.3286261113800748 1102 104-105 0.23991223730418199 1102 106-107 0.14098123574176213 1102 108-109 0.1369190234268487 1102 110-111 0.18682634159672773 1102 112-113 0.12141524763590184 1102 114-115 0.09723502869342582 1102 116-117 0.13125340475452774 1102 118-119 -0.03894338085592608 1102 120-121 -0.018328120918592816 1102 122-123 -0.06649375441063654 1102 124-125 -0.24391520291516855 1103 1 0.1298390367108624 1103 2 0.17873435682750483 1103 3 0.22699360081515607 1103 4 0.16424125788770993 1103 5 0.041765464216105386 1103 6 0.05693413973540373 1103 7 0.09594959188154206 1103 8 0.13915853825254487 1103 9 0.25174641131602016 1103 10-11 0.25455936474286034 1103 12-13 0.24875889876595636 1103 14-15 0.40127391140777036 1103 16-17 0.3717286986823538 1103 18-19 0.3316455142963761 1103 20-21 0.20898777599595064 1103 22-23 0.3057203681911247 1103 24-25 0.33771718039033516 1103 26-27 0.319832116789442 1103 28-29 0.3772078541388879 1103 30-31 0.4245628252086391 1103 32-33 0.3470156021109787 1103 34-35 0.3690388928696251 1103 36-37 0.35688500907608756 1103 38-39 0.42231981727489654 1103 40-41 0.36925987110581815 1103 42-43 0.38132522033432537 1103 44-45 0.38241270916024916 1103 46-47 0.5281275592895938 1103 48-49 0.41257117044458624 1103 50-51 0.2584617364318902 1103 52-53 0.4764696552943093 1103 54-55 0.48026621389758617 1103 56-57 0.43122125868519845 1103 58-59 0.35591451465113977 1103 60-61 0.3900467150991318 1103 62-63 0.3448354995235263 1103 64-65 0.38642590701061863 1103 66-67 0.2672551222181596 1103 68-69 0.3261383695778477 1103 70-71 0.3191463622495121 1103 72-73 0.37803685478874627 1103 74-75 0.3913354073173565 1103 76-77 0.4175346239526405 1103 78-79 0.4405120611582376 1103 80-81 0.5167129985667813 1103 82-83 0.5007061265022159 1103 84-85 0.5453092949076606 1103 86-87 0.5547715226651775 1103 88-89 0.5461984999807825 1103 90-91 0.6022379893296126 1103 92-93 0.5931723086389056 1103 94-95 0.4803126392413759 1103 96-97 0.5197587606367016 1103 98-99 0.4828997997122251 1103 100-101 0.4324942471598874 1103 102-103 0.5641441685786006 1103 104-105 0.5973449142229761 1103 106-107 0.5378372884404854 1103 108-109 0.5964141872985351 1103 110-111 0.6294095531570463 1103 112-113 0.5665965154669479 1103 114-115 0.4934913782863539 1103 116-117 0.5321440873792582 1103 118-119 0.5208964586356437 1103 120-121 0.4839244479121554 1103 122-123 0.4256808796904501 1103 124-125 0.20678992082940795 1104 1 0.049625523576992236 1104 2 0.07153250263449706 1104 3 0.11089302489157404 1104 4 0.08886831224928216 1104 5 0.12570000558427097 1104 6 0.18318220828435727 1104 7 0.20136327357840855 1104 8 0.21363809998874217 1104 9 0.22769949461423522 1104 10-11 0.20972020193909202 1104 12-13 0.2285025425361482 1104 14-15 0.27568253621463157 1104 16-17 0.29498477397382317 1104 18-19 0.21213996576428684 1104 20-21 0.17801221763101438 1104 22-23 0.18707238392367742 1104 24-25 0.1596640830452074 1104 26-27 0.09660132594802917 1104 28-29 0.21090500261480116 1104 30-31 0.2925520090827831 1104 32-33 0.3043712700418695 1104 34-35 0.21729847989027462 1104 36-37 0.24990088209195704 1104 38-39 0.304033541112517 1104 40-41 0.19081767416805206 1104 42-43 0.11765195161680708 1104 44-45 0.19207909488857666 1104 46-47 0.1945884386265746 1104 48-49 0.2300153339506963 1104 50-51 0.20047395724942163 1104 52-53 0.279897805315386 1104 54-55 0.27283820434568185 1104 56-57 0.25975897465654896 1104 58-59 0.22031330661630477 1104 60-61 0.1757392280925245 1104 62-63 0.04936773719735754 1104 64-65 0.02462228290610824 1104 66-67 0.03489945428727026 1104 68-69 0.02898357255579498 1104 70-71 0.005384597956776815 1104 72-73 0.14765126692527986 1104 74-75 0.3060874792375685 1104 76-77 0.22377707373491518 1104 78-79 0.1526240150843492 1104 80-81 0.17206136210099032 1104 82-83 0.2381067445665508 1104 84-85 0.22021588291159588 1104 86-87 0.09648173477361155 1104 88-89 0.09545050166641644 1104 90-91 0.08013235651601391 1104 92-93 0.21755845837903465 1104 94-95 0.09695050972880281 1104 96-97 -0.025654218714329602 1104 98-99 0.03351505466376281 1104 100-101 0.10906328775547536 1104 102-103 0.058550686347949465 1104 104-105 0.1404429617015559 1104 106-107 0.150912860852344 1104 108-109 0.19266436288499378 1104 110-111 0.18908506938123182 1104 112-113 0.16393462475195975 1104 114-115 0.08280869813464875 1104 116-117 0.10900881557141773 1104 118-119 0.1681257043162141 1104 120-121 0.1732775873980401 1104 122-123 0.15329360619697852 1104 124-125 -0.04022510937146251 1105 1 0.06612209925159362 1105 2 0.08216881602883319 1105 3 0.08472253854409217 1105 4 0.08478598922999225 1105 5 0.08272436216886803 1105 6 0.13199110730771224 1105 7 0.09177511605062705 1105 8 0.11993608381536802 1105 9 0.14710383507834734 1105 10-11 0.18036309066927458 1105 12-13 0.22681334942777198 1105 14-15 0.1990888061245073 1105 16-17 0.2149355123186112 1105 18-19 0.253116558589511 1105 20-21 0.18685098640517595 1105 22-23 0.20788097854192245 1105 24-25 0.19980936223711865 1105 26-27 0.2066603601207504 1105 28-29 0.16968386483576126 1105 30-31 0.2920152398696416 1105 32-33 0.2506868082055007 1105 34-35 0.25295111561620587 1105 36-37 0.2332525678976296 1105 38-39 0.309307614753493 1105 40-41 0.1851505652787253 1105 42-43 0.13468170025522852 1105 44-45 0.0823006841709173 1105 46-47 0.020222550188258026 1105 48-49 0.057890952911606064 1105 50-51 0.13738901569659134 1105 52-53 0.06618976136411447 1105 54-55 0.028143020117951778 1105 56-57 -0.011534533579791173 1105 58-59 -0.09352243898826629 1105 60-61 -0.01857493932006804 1105 62-63 -0.01983303235359557 1105 64-65 -0.140575373917045 1105 66-67 -0.041626875130098995 1105 68-69 -0.08256396081159778 1105 70-71 -0.09526339976761733 1105 72-73 0.03239054813016651 1105 74-75 -0.018263235348776163 1105 76-77 -0.03438699226345676 1105 78-79 0.08706647811142432 1105 80-81 0.05267841351992786 1105 82-83 0.040379040500283736 1105 84-85 0.1382352033550447 1105 86-87 0.1253491760955825 1105 88-89 0.15776561384851462 1105 90-91 0.11192876889769465 1105 92-93 0.166148218209905 1105 94-95 0.020335898191014223 1105 96-97 0.12792534864991367 1105 98-99 0.1320869724576781 1105 100-101 0.051595687641693644 1105 102-103 0.1704955472612646 1105 104-105 0.07470654032693247 1105 106-107 0.06769043120774043 1105 108-109 0.047043527283701536 1105 110-111 0.14073407730365517 1105 112-113 0.10390521903332228 1105 114-115 0.1880710317617158 1105 116-117 0.08553180395715998 1105 118-119 0.16350359587468333 1105 120-121 0.21031953544221338 1105 122-123 0.2865120219633077 1105 124-125 0.12898480161324954 1106 1 0.05637620115170705 1106 2 0.08941963943912867 1106 3 0.1085476275249917 1106 4 0.08672068591955195 1106 5 0.17481686041197975 1106 6 0.03953497610395118 1106 7 0.07718256098986842 1106 8 0.07419698422583565 1106 9 0.08991854135783228 1106 10-11 0.1517440403127992 1106 12-13 0.11864490982760856 1106 14-15 0.15858606573125655 1106 16-17 0.085450538693145 1106 18-19 0.09418834445961011 1106 20-21 0.06519916139997406 1106 22-23 0.047028205773756326 1106 24-25 0.12038006990874806 1106 26-27 0.08020443568867108 1106 28-29 0.10972910059781782 1106 30-31 0.18293393002450387 1106 32-33 0.1447972020030619 1106 34-35 0.0929387984409189 1106 36-37 0.2787803281102157 1106 38-39 0.1402261979574888 1106 40-41 0.007205372252450104 1106 42-43 0.0987230349842605 1106 44-45 0.19492518723929209 1106 46-47 0.15151822433747952 1106 48-49 0.15291178827812502 1106 50-51 0.03571048862398385 1106 52-53 0.025078180857512677 1106 54-55 0.038392478567764954 1106 56-57 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-0.5878511228960406 2216 40-41 -0.983300161454217 2216 42-43 -0.8827244337153246 2216 44-45 -0.7233890816415496 2216 46-47 -0.8157450495830219 2216 48-49 -0.791476448603035 2216 50-51 -0.6485162855461368 2216 52-53 -0.7835771497524888 2216 54-55 -0.7290500254186512 2216 56-57 -0.7174030799391318 2216 58-59 -0.6503064865698605 2216 60-61 -0.7717217016692857 2216 62-63 -0.6796069099188813 2216 64-65 -0.6649741743894708 2216 66-67 -0.6490737341107007 2216 68-69 -0.7047265062870238 2216 70-71 -0.7544230313198881 2216 72-73 -0.8469712852193965 2216 74-75 -0.8693035833216385 2216 76-77 -0.8069586605406442 2216 78-79 -0.6540850084388339 2216 80-81 -0.7595283526745717 2216 82-83 -0.7773097465136587 2216 84-85 -0.6167867571883932 2216 86-87 -0.677908575014925 2216 88-89 -0.6304905517082666 2216 90-91 -0.5634559463643249 2216 92-93 -0.6010625305959394 2216 94-95 -0.6801330262042917 2216 96-97 -0.6018875560096149 2216 98-99 -0.6181421213296971 2216 100-101 -0.7235965139308753 2216 102-103 -0.7511631167286872 2216 104-105 -0.7062997744217121 2216 106-107 -0.6916275060243073 2216 108-109 -0.6368519909676422 2216 110-111 -0.5871187333712413 2216 112-113 -0.4933953445249095 2216 114-115 -0.5143230295280539 2216 116-117 -0.5197077644725958 2216 118-119 -0.48576817302899045 2216 120-121 -0.42988711089940423 2216 122-123 -0.43375135474178705 2216 124-125 -0.43169196765247975 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 216.0 3 9.0 4 56.0 5 168.0 6 393.0 7 764.0 8 1340.0 9 2327.0 10 4126.0 11 5631.0 12 7151.0 13 10590.0 14 17532.0 15 14792.0 16 6066.0 17 5070.0 18 5072.0 19 4449.0 20 4220.0 21 4186.0 22 4395.0 23 4742.0 24 5636.0 25 7490.0 26 9717.0 27 12491.0 28 15929.0 29 20203.0 30 25193.0 31 31797.0 32 40494.0 33 52772.0 34 72530.0 35 111412.0 36 219592.0 37 465170.0 38 229042.0 39 1312.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.73651741545135 18.471637773783513 13.171249421404827 22.62059538936031 2 16.22246019345891 20.98049611151098 38.724505380685706 24.0725383143444 3 18.608570475571863 26.677176412759156 30.318206555132278 24.3960465565367 4 12.339448413882696 16.9444727279111 36.98218141600688 33.73389744219932 5 12.244440572509323 39.74537498911018 33.734406483867616 14.275777954512881 6 30.779247048115092 37.3164465570473 17.22220817566068 14.682098219176929 7 27.74067377069326 34.6317434123905 20.53206467356003 17.095518143356216 8 27.461264329477032 34.09279707880554 19.563365693520353 18.882572898197076 9 28.384530309148044 14.49551463230518 19.42882221793094 37.691132840615836 10-11 24.974492214244336 27.131049979811454 26.919263381493252 20.97519442445096 12-13 26.374558924213964 24.844864210101296 27.811912996155396 20.968663869529344 14-15 23.26597967101452 25.075926478591366 26.69792672436494 24.960167126029177 16-17 21.442831311553114 28.99131014869301 27.277776802485825 22.28808173726805 18-19 21.630391657742745 26.191246949774417 30.897003317943227 21.281358074539614 20-21 22.778155997192552 25.259037386140644 31.063560406595037 20.899246210071762 22-23 22.517493811772553 25.510629707002792 30.84479398908063 21.127082492144023 24-25 21.864157435528327 25.6566192089602 31.00159752822007 21.477625827291398 26-27 21.55624372397424 25.982887077180195 30.78756662242727 21.673302576418294 28-29 21.71518655124651 25.95966848597947 30.869881975812323 21.45526298696169 30-31 21.575634412256665 25.45791159729059 31.03335922150113 21.933094768951612 32-33 21.544764687521614 25.67358659957748 30.843154624284054 21.938494088616853 34-35 22.18714681008613 25.612459179628594 30.613953132367723 21.586440877917553 36-37 22.21968646314274 25.26492635570458 30.389059564980776 22.1263276161719 38-39 21.938135280796306 25.496725944911606 30.39720520337763 22.167933570914453 40-41 22.080812542330154 25.35631061177671 30.468792516678388 22.09408432921475 42-43 22.032512332566753 25.42197566841634 30.167055808156174 22.378456190860735 44-45 22.05298176008988 25.65517967803662 30.052209328862595 22.239629233010902 46-47 22.682864315432823 26.134508365079085 28.81207099345189 22.370556326036198 48-49 22.676732637113854 26.138285142886037 28.525418658607148 22.65956356139296 50-51 22.729175078559766 26.466267577199236 28.34945490932711 22.455102434913893 52-53 22.821937046854977 26.310096027245756 28.309218264487473 22.558748661411794 54-55 22.160279479662236 26.45727928655443 28.47694117233994 22.905500061443394 56-57 22.366280696691078 26.583581125847 28.40950385671536 22.64063432074656 58-59 22.446016693649103 25.92360863143045 28.52312150803908 23.107253166881367 60-61 22.12299211769043 26.17253304776785 28.9960149570774 22.708459877464318 62-63 22.062918034513633 26.144865965626813 29.02606955392097 22.766146445938592 64-65 21.888243245615573 26.024963572845532 28.9541281182522 23.132665063286694 66-67 21.89891684075628 26.233379562171937 28.784860347945155 23.082843249126626 68-69 22.018265196097538 25.997481171104457 28.840731296010148 23.143522336787857 70-71 21.952764376276924 26.295061315304302 28.468017978004646 23.284156330414124 72-73 22.062800831558203 26.41219010114295 28.07653537545639 23.44847369184245 74-75 22.125625405965277 26.55843266681881 27.922124888085246 23.393817039130663 76-77 22.34402847043061 26.272089793086593 27.997070376960746 23.386811359522046 78-79 22.26901673015817 26.532521110194335 27.963309516703827 23.235152642943664 80-81 22.210015438129336 26.68826670570965 27.766513128027633 23.335204728133384 82-83 22.19781261520636 26.552920316696806 27.663219984902483 23.58604708319435 84-85 22.168916665203728 26.474974983761392 27.648403349542683 23.707705001492197 86-87 22.269508277302812 26.374313150641644 27.551217456945736 23.80496111510981 88-89 22.163544757123045 26.475010094271724 27.639836385021855 23.72160876358338 90-91 22.038902445447047 26.645120516826715 27.480820883731543 23.8351561539947 92-93 22.004669697874057 26.10592840966943 27.69899759493004 24.190404297526463 94-95 21.948106665730386 26.064427786457877 27.629408563453474 24.358056984358267 96-97 22.171058406333934 25.952846584625107 27.515615399469834 24.360479609571126 98-99 21.950404262699834 26.125643751658973 27.290681523572513 24.633270462068683 100-101 21.739796007934974 26.15848884363534 27.47843336902902 24.623281779400664 102-103 21.68923687305795 25.810157470638835 27.504555588715483 24.99605006758773 104-105 21.586412113232388 25.985692341452708 27.46578889620364 24.962106649111256 106-107 21.760862314133735 26.062180713796675 27.231220265786565 24.945736706283025 108-109 21.79675968254102 26.137559003916977 27.052830713355775 25.012850600186226 110-111 21.677190244971207 26.320228338768047 27.158451276645874 24.844130139614872 112-113 21.59405227954988 26.522795498832576 27.1455857310886 24.73756649052894 114-115 21.414040693081475 26.599792847989047 26.86245457577726 25.12371188315222 116-117 21.394273475764972 26.493443112195635 27.013535101732707 25.09874831030669 118-119 21.240243666941698 26.942401207801552 26.655197233291787 25.16215789196496 120-121 21.201064554410948 27.04916190917581 26.74096076737801 25.00881276903523 122-123 21.041904322518874 27.121154188483604 26.641997857542364 25.194943631455153 124-125 21.0704917474472 27.158937260655957 26.52891404206732 25.241656949829522 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 245.0 1 205.5 2 393.5 3 606.0 4 393.0 5 301.0 6 693.5 7 1723.5 8 3077.0 9 3630.5 10 2760.5 11 1789.0 12 1680.0 13 1853.0 14 2057.5 15 2279.0 16 2466.5 17 2603.5 18 2826.5 19 3010.0 20 3196.0 21 3551.0 22 3983.5 23 4724.0 24 5872.0 25 7084.0 26 8716.5 27 10768.5 28 12947.0 29 15410.0 30 18349.5 31 21104.5 32 24120.5 33 25848.0 34 25743.0 35 26426.5 36 28482.0 37 31389.0 38 33569.5 39 35033.5 40 36491.5 41 38878.0 42 41551.5 43 43836.5 44 46670.0 45 49478.5 46 50423.5 47 50858.5 48 51243.5 49 50552.5 50 50342.0 51 49488.0 52 48808.5 53 50085.0 54 51867.0 55 49754.0 56 44600.5 57 40581.5 58 37873.5 59 34037.0 60 28056.0 61 23223.0 62 19179.5 63 14778.5 64 11303.5 65 8385.5 66 5823.5 67 4591.0 68 3462.0 69 2079.5 70 1335.0 71 952.5 72 741.5 73 577.0 74 510.5 75 430.0 76 242.5 77 98.0 78 48.5 79 23.0 80 4.5 81 3.5 82 3.0 83 3.0 84 4.5 85 2.5 86 0.5 87 0.5 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025841335603813 2 0.0 3 0.0 4 0.0 5 0.05105068202166319 6 0.016782823938345945 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 3.15994592981409E-4 22-23 0.0 24-25 0.0 26-27 3.5110510331267666E-4 28-29 1.7555255165633833E-4 30-31 7.022102066253533E-5 32-33 3.511051033126766E-5 34-35 3.511051033126766E-5 36-37 0.0 38-39 0.0 40-41 1.05331530993803E-4 42-43 0.0 44-45 0.0 46-47 0.0 48-49 2.10663061987606E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 3.511051033126766E-5 58-59 3.511051033126766E-5 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 3.511051033126766E-5 70-71 1.05331530993803E-4 72-73 1.05331530993803E-4 74-75 0.0 76-77 7.022102066253533E-5 78-79 0.0 80-81 2.457735723188737E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 7.022102066253533E-5 100-101 0.0 102-103 0.0 104-105 8.777627582816916E-4 106-107 0.0 108-109 0.0011937573512631006 110-111 0.0035461615434580346 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 3.5110510331267666E-4 122-123 6.670996962940856E-4 124-125 3.511051033126766E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1424075.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.62353548057709 #Duplication Level Percentage of deduplicated Percentage of total 1 78.80398201165872 49.349839635178675 2 12.077270619943354 15.126427703531075 3 4.095606761517283 7.69444126033107 4 1.8479279300089118 4.628951211618498 5 1.0134618907431154 3.173328333658211 6 0.5765977656785258 2.166515438219438 7 0.36947086961484515 1.6196300478655445 8 0.26676032505893693 1.3364379744910888 9 0.17558493834083097 0.989617465443777 >10 0.7245095092664091 7.444795307925651 >50 0.03295903723715367 1.4140875574881522 >100 0.013020177183636829 1.5932830152417699 >500 0.0018309624095869813 0.7117921148240279 >1k 6.103208031956604E-4 0.883529320978098 >5k 4.0688053546377356E-4 1.8673236132049003 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7865 0.5522883275108404 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6792 0.47694117233994 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4020 0.28228850306339204 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1727 0.12127170268419851 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 7.022102066253533E-5 0.0 0.0 0.0 0.0 7 7.022102066253533E-5 0.0 0.0 0.0 0.0 8 7.022102066253533E-5 0.0 0.0 0.0 0.0 9 7.022102066253533E-5 0.0 0.0 0.0 0.0 10-11 7.022102066253533E-5 0.0 0.0 0.0 0.0 12-13 7.022102066253533E-5 0.0 0.0 0.0 0.0 14-15 7.022102066253533E-5 0.0 0.0 0.0 0.0 16-17 7.022102066253533E-5 0.0 0.0 3.511051033126766E-5 0.0 18-19 7.022102066253533E-5 0.0 0.0 7.022102066253533E-5 0.0 20-21 7.022102066253533E-5 0.0 0.0 1.0533153099380299E-4 0.0 22-23 7.022102066253533E-5 0.0 0.0 6.670996962940855E-4 0.0 24-25 1.4044204132507065E-4 0.0 0.0 0.0016150834752383125 0.0 26-27 1.4044204132507065E-4 0.0 0.0 0.00315994592981409 0.0 28-29 1.4044204132507065E-4 0.0 0.0 0.008988290644804522 0.0 30-31 1.4044204132507065E-4 0.0 0.0 0.025630672541825397 0.0 32-33 1.7555255165633833E-4 0.0 0.0 0.058494110211891936 0.0 34-35 2.1066306198760598E-4 0.0 0.0 0.10399733160121483 0.0 36-37 2.808840826501413E-4 0.0 0.0 0.18050313361304707 0.0 38-39 3.5110510331267666E-4 0.0 0.0 0.2959816020925864 0.0 40-41 3.5110510331267666E-4 0.0 0.0 0.4221687762231624 0.0 42-43 3.5110510331267666E-4 0.0 0.0 0.5465302038165125 0.0 44-45 4.2132612397521196E-4 0.0 0.0 0.6920632691396169 0.0 46-47 4.564366343064796E-4 0.0 0.0 0.8687744676368871 0.0 48-49 4.915471446377473E-4 0.0 0.0 1.0600565279216334 0.0 50-51 4.915471446377473E-4 0.0 0.0 1.288274845074873 0.0 52-53 4.915471446377473E-4 0.0 0.0 1.4924424626511947 0.0 54-55 4.915471446377473E-4 0.0 0.0 1.7040886189280762 0.0 56-57 5.617681653002826E-4 0.0 0.0 1.9580429401541353 0.0 58-59 5.617681653002826E-4 0.0 0.0 2.2427189579200535 0.0 60-61 5.617681653002826E-4 0.0 0.0 2.5623650439759142 0.0 62-63 5.617681653002826E-4 0.0 0.0 2.879237399715605 0.0 64-65 5.617681653002826E-4 0.0 0.0 3.184663729087302 0.0 66-67 6.31989185962818E-4 0.0 0.0 3.530291592788301 0.0 68-69 6.31989185962818E-4 0.0 0.0 3.9132068184611066 0.0 70-71 6.31989185962818E-4 0.0 0.0 4.355739690676404 0.0 72-73 6.31989185962818E-4 0.0 0.0 4.805470217509612 0.0 74-75 6.31989185962818E-4 0.0 0.0 5.235644190088303 0.0 76-77 6.31989185962818E-4 0.0 0.0 5.693239471235715 0.0 78-79 6.31989185962818E-4 0.0 0.0 6.2052560433965915 0.0 80-81 9.479837789442269E-4 0.0 0.0 6.778680898126854 0.0 82-83 9.830942892754945E-4 0.0 0.0 7.378017309481593 0.0 84-85 9.830942892754945E-4 0.0 0.0 7.953864789424715 0.0 86-87 9.830942892754945E-4 0.0 0.0 8.523989256183839 0.0 88-89 9.830942892754945E-4 0.0 0.0 9.13916050769798 0.0 90-91 0.0010533153099380299 0.0 0.0 9.839720520337764 0.0 92-93 0.0011235363306005652 0.0 0.0 10.565489879395397 0.0 94-95 0.0011235363306005652 0.0 0.0 11.276583045134561 0.0 96-97 0.0011235363306005652 0.0 0.0 11.965556589365026 0.0 98-99 0.0011235363306005652 0.0 0.0 12.693081473939223 0.0 100-101 0.001158646840931833 0.0 0.0 13.495426856029352 0.0 102-103 0.0011937573512631006 0.0 0.0 14.349770903920088 0.0 104-105 0.0013693099029194388 0.0 0.0 15.189087653389041 0.0 106-107 0.0014044204132507066 0.0 0.0 15.989607288941945 0.0 108-109 0.0014044204132507066 0.0 0.0 16.80796306374313 0.0 110-111 0.0014044204132507066 0.0 0.0 17.678809051489562 0.0 112-113 0.0014044204132507066 0.0 0.0 18.617628987237328 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4135 0.0 82.52349 1 GTATCAA 8020 0.0 59.403786 1 GTGGTAT 1170 0.0 49.88141 1 ATCAACG 10335 0.0 45.79658 3 TATCAAC 10450 0.0 45.406544 2 TCAACGC 10465 0.0 45.28457 4 CAACGCA 10640 0.0 44.483803 5 AACGCAG 10900 0.0 43.622807 6 TGGTATC 1360 0.0 40.27405 2 ACGCAGA 12870 0.0 36.98395 7 GTACATG 10590 0.0 36.94604 1 CGCAGAG 13035 0.0 36.5158 8 TACATGG 10750 0.0 35.832096 2 ACATGGG 11135 0.0 34.00504 3 GCAGAGT 14100 0.0 33.6311 9 CATGGGA 8915 0.0 32.322163 4 GAGTACT 8365 0.0 30.371326 12-13 CAGAGTA 13760 0.0 29.165228 10-11 ATGGGAG 3085 0.0 28.947596 5 ATGGGAT 3620 0.0 28.287613 5 >>END_MODULE