FastQCFastQC Report
Thu 26 May 2016
SRR1364392_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364392_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1275005
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20950.046.321721
GGTATCA13550.043.4987751
ATCAACG22700.041.6868443
TCAACGC22900.041.3227654
CAACGCA23600.040.097095
AACGCAG24600.038.4610986
GTACATG61450.036.4289861
TATCAAC26350.036.1382522
TACATGG65150.034.3479772
ACATGGG66250.033.0593
ACGCAGA31250.030.2718247
CGCAGAG32350.029.0574328
CATGGGA42600.028.3605214
ATAGCGA1556.8268782E-626.8736086
TGCGCGA450.00953200226.4439352-53
GAGTACA46650.025.7798791
CATGGGG29300.025.1873114
AGTACAT47050.024.666232
ATGGGGA17250.023.8060725
GCAGAGT39200.023.2209429
Produced by FastQC (version 0.11.5)