##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364392_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1275005 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.99878196556092 31.0 31.0 34.0 28.0 34.0 2 31.056608405457233 33.0 31.0 34.0 27.0 34.0 3 31.130463802102735 33.0 31.0 34.0 27.0 34.0 4 34.52282775361665 37.0 35.0 37.0 32.0 37.0 5 34.599945098254516 37.0 35.0 37.0 32.0 37.0 6 34.58833337908479 37.0 35.0 37.0 31.0 37.0 7 34.571073054615475 37.0 35.0 37.0 31.0 37.0 8 34.51000505880369 37.0 35.0 37.0 31.0 37.0 9 36.02717479539296 39.0 37.0 39.0 31.0 39.0 10-11 35.95950800192941 39.0 37.0 39.0 30.5 39.0 12-13 35.96602601558425 39.0 37.0 39.0 31.0 39.0 14-15 37.27813420339528 40.0 38.0 41.0 31.0 41.0 16-17 37.280468704044296 40.0 38.0 41.0 31.0 41.0 18-19 37.276460876624014 40.0 38.0 41.0 31.0 41.0 20-21 37.31558935062999 40.0 38.0 41.0 31.0 41.0 22-23 37.2803726259897 40.0 38.0 41.0 31.0 41.0 24-25 37.292904733706926 40.0 38.0 41.0 31.0 41.0 26-27 37.25941113956416 40.0 38.0 41.0 30.0 41.0 28-29 37.27027031266544 40.0 38.0 41.0 30.0 41.0 30-31 37.17966831502622 40.0 37.5 41.0 30.0 41.0 32-33 37.148654711157995 40.0 37.0 41.0 30.0 41.0 34-35 37.14314257591147 40.0 37.0 41.0 30.0 41.0 36-37 37.07832283010655 40.0 37.0 41.0 30.0 41.0 38-39 36.98654593511398 40.0 37.0 41.0 30.0 41.0 40-41 36.923675201273724 40.0 37.0 41.0 30.0 41.0 42-43 36.789098866278955 40.0 37.0 41.0 30.0 41.0 44-45 36.65472213834455 40.0 36.5 41.0 30.0 41.0 46-47 36.54337433970847 40.0 36.0 41.0 30.0 41.0 48-49 36.395536488092205 40.0 36.0 41.0 29.0 41.0 50-51 36.27831537915537 40.0 35.5 41.0 29.0 41.0 52-53 36.12017521499916 39.5 35.0 41.0 29.0 41.0 54-55 35.93076066368367 39.0 35.0 41.0 28.5 41.0 56-57 35.73936298289026 39.0 35.0 41.0 28.0 41.0 58-59 35.521241093172186 39.0 35.0 41.0 28.0 41.0 60-61 35.27547578244791 38.0 34.5 40.5 28.0 41.0 62-63 35.01048388045537 38.0 34.0 40.0 28.0 41.0 64-65 34.6759059768393 37.0 34.0 40.0 27.0 41.0 66-67 34.36988639260238 37.0 34.0 40.0 26.5 41.0 68-69 34.05167705224686 36.0 34.0 39.0 26.0 41.0 70-71 33.7004082336932 36.0 33.0 39.0 26.0 41.0 72-73 33.34180218901102 35.0 33.0 38.5 26.0 40.0 74-75 32.990114156415075 35.0 33.0 37.5 26.0 39.5 76-77 32.75911741522582 35.0 33.0 37.0 26.0 39.0 78-79 32.600251371563246 35.0 33.0 37.0 26.0 39.0 80-81 32.33481398112164 35.0 33.0 36.0 26.0 38.5 82-83 32.051539013572494 35.0 33.0 36.0 25.0 37.0 84-85 31.806087034952803 35.0 33.0 35.5 25.0 37.0 86-87 31.598107458402122 35.0 33.0 35.0 24.5 36.5 88-89 31.348103340771214 35.0 32.0 35.0 24.0 36.0 90-91 31.232945360998585 35.0 32.0 35.0 23.5 36.0 92-93 31.092454539393962 35.0 32.0 35.0 23.0 36.0 94-95 30.995515703859986 35.0 32.0 35.0 23.0 35.0 96-97 30.90294547864518 35.0 32.0 35.0 22.0 35.0 98-99 30.782102423127753 35.0 32.0 35.0 20.0 35.0 100-101 30.703135281822426 35.0 32.0 35.0 20.0 35.0 102-103 29.670353841749638 33.5 30.0 34.5 18.5 35.0 104-105 30.394560413488577 34.5 31.5 35.0 19.0 35.0 106-107 30.74043827279109 35.0 32.0 35.0 20.0 35.0 108-109 30.779698510986233 35.0 32.0 35.0 19.5 35.0 110-111 30.719067376206368 35.0 32.0 35.0 19.0 35.0 112-113 30.641186112995634 35.0 32.0 35.0 18.0 35.0 114-115 30.550059803687045 35.0 32.0 35.0 17.5 35.0 116-117 30.43524692060031 35.0 32.0 35.0 11.5 35.0 118-119 30.28065929153219 35.0 32.0 35.0 3.5 35.0 120-121 30.11869718157968 35.0 32.0 35.0 2.0 35.0 122-123 29.955285665546413 35.0 32.0 35.0 2.0 35.0 124-125 29.158633887710245 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16015755249229002 1101 2 0.24217195895787924 1101 3 0.19305914345712694 1101 4 0.26369007398896116 1101 5 0.1402606794778265 1101 6 -0.06909824072528892 1101 7 -0.014746351969854743 1101 8 0.06441381378616029 1101 9 0.1311138985645499 1101 10-11 0.27848257100603035 1101 12-13 0.3033725743192832 1101 14-15 0.35191687617925993 1101 16-17 0.26880044726058827 1101 18-19 0.12550603921619796 1101 20-21 0.14071298706935664 1101 22-23 0.25888260145987374 1101 24-25 0.24378756522312983 1101 26-27 0.385896866660417 1101 28-29 0.3289324568452372 1101 30-31 0.3859549694289939 1101 32-33 0.29286554337332404 1101 34-35 0.3750245716918599 1101 36-37 0.23051326612027623 1101 38-39 0.35215288984822735 1101 40-41 0.3085726328484881 1101 42-43 0.43073360812866923 1101 44-45 0.3667181173516596 1101 46-47 0.3797219456821921 1101 48-49 0.3783979892583389 1101 50-51 0.28652736818526137 1101 52-53 0.079464179806088 1101 54-55 0.2659933190182855 1101 56-57 0.19468062776126516 1101 58-59 0.2187380991576191 1101 60-61 0.22814210679113955 1101 62-63 0.1745853571085405 1101 64-65 0.29906018143388025 1101 66-67 0.1800759677343251 1101 68-69 0.1690153101764338 1101 70-71 0.19660986193471786 1101 72-73 0.35713630095393256 1101 74-75 0.3510676597847109 1101 76-77 0.4470316442226405 1101 78-79 0.24341584621040369 1101 80-81 0.286072155575404 1101 82-83 0.2484037412652924 1101 84-85 0.24636378534550119 1101 86-87 0.22855954453167726 1101 88-89 0.2820509255981669 1101 90-91 0.23921391733144048 1101 92-93 0.2306742069984722 1101 94-95 0.25184207320830154 1101 96-97 0.17017332842430832 1101 98-99 0.10725719966823632 1101 100-101 0.09741718050158354 1101 102-103 -0.05460974850688771 1101 104-105 0.16082634773061244 1101 106-107 0.13310317673960625 1101 108-109 0.12142913696296631 1101 110-111 0.22929532928042207 1101 112-113 0.2810567004018658 1101 114-115 0.13844746634416794 1101 116-117 0.08772726292696476 1101 118-119 0.1235988829492527 1101 120-121 -0.0436983260117394 1101 122-123 -0.04174904009552094 1101 124-125 -0.1025388076052387 1102 1 0.0038355112404282465 1102 2 0.07560320390106767 1102 3 0.14284338613720848 1102 4 0.1376877991563461 1102 5 0.2257304793160131 1102 6 0.11786720292570152 1102 7 0.05968640561976457 1102 8 0.13600185801641373 1102 9 0.07709776366932175 1102 10-11 0.025807590646984124 1102 12-13 0.06794733305804357 1102 14-15 0.0962552985711227 1102 16-17 0.07426737424314922 1102 18-19 0.13279898840769278 1102 20-21 0.12026792896731564 1102 22-23 0.06683748003776202 1102 24-25 0.1407068672951013 1102 26-27 0.06185311717342046 1102 28-29 0.022003598131099977 1102 30-31 0.1641321132242055 1102 32-33 0.10716210415473881 1102 34-35 0.02266472600524594 1102 36-37 -0.051341132866596695 1102 38-39 -0.04138582334697105 1102 40-41 -0.18881365483576218 1102 42-43 -0.2366181466584436 1102 44-45 -0.22491776871909508 1102 46-47 -0.2745108259785525 1102 48-49 -0.26704853458635824 1102 50-51 -0.19230554887715812 1102 52-53 -0.18401068563873224 1102 54-55 -0.15619685229771818 1102 56-57 -0.18414437429077424 1102 58-59 -0.18053254729352375 1102 60-61 -0.12293814555111737 1102 62-63 -0.20377265933123567 1102 64-65 -0.2186890827632979 1102 66-67 -0.24332930424888133 1102 68-69 -0.21228064085603648 1102 70-71 -0.2362561926378106 1102 72-73 -0.11207548127188716 1102 74-75 -0.15182337104279497 1102 76-77 -0.06407340848546283 1102 78-79 -0.006722226748401283 1102 80-81 -0.11223285960969775 1102 82-83 -0.15102755058166295 1102 84-85 -0.06867473437166538 1102 86-87 -0.0696296769830802 1102 88-89 0.06432276809532311 1102 90-91 -0.08851558865465137 1102 92-93 -0.04714031206204439 1102 94-95 -0.18522098890400684 1102 96-97 -0.20883195997521753 1102 98-99 -0.14556376865999354 1102 100-101 -0.27776926196508356 1102 102-103 -0.435765867689355 1102 104-105 -0.2993476652382796 1102 106-107 -0.23411285027696493 1102 108-109 -0.08942058337333236 1102 110-111 -0.12299914428344394 1102 112-113 -0.24103526615575177 1102 114-115 -0.18417040868074963 1102 116-117 -0.24565913029075048 1102 118-119 -0.2685523582416316 1102 120-121 -0.29311759876886256 1102 122-123 -0.42791008417333387 1102 124-125 -0.5426221790478785 1103 1 0.3847829417467743 1103 2 0.3227570510341202 1103 3 0.32807351822924957 1103 4 0.3170877202320952 1103 5 0.3350023818572865 1103 6 0.26753262349438245 1103 7 0.26598846486387373 1103 8 0.14994938611726383 1103 9 0.3582272291484472 1103 10-11 0.42697830041365137 1103 12-13 0.3526421804923672 1103 14-15 0.4373300686936261 1103 16-17 0.345259740655365 1103 18-19 0.37231818694461793 1103 20-21 0.3824226883968507 1103 22-23 0.4399264310665316 1103 24-25 0.4292100221436357 1103 26-27 0.448729414013485 1103 28-29 0.4342290074976276 1103 30-31 0.6231869740671669 1103 32-33 0.6153441306410343 1103 34-35 0.45311484884702935 1103 36-37 0.3262891451928809 1103 38-39 0.4093374368187668 1103 40-41 0.32743795229118433 1103 42-43 0.30673715251115397 1103 44-45 0.32968529744573516 1103 46-47 0.29734419101561826 1103 48-49 0.34542701374625295 1103 50-51 0.43268861666858527 1103 52-53 0.3638318392584736 1103 54-55 0.3134533774736994 1103 56-57 0.3686839569704432 1103 58-59 0.3095149345560202 1103 60-61 0.24330539942086205 1103 62-63 0.34085455086611915 1103 64-65 0.25192542317510913 1103 66-67 0.2998424748141204 1103 68-69 0.2626121112666482 1103 70-71 0.34081482646101335 1103 72-73 0.36176282584529673 1103 74-75 0.3605615841273817 1103 76-77 0.3345895467610873 1103 78-79 0.40055556540924186 1103 80-81 0.3412315332463862 1103 82-83 0.3119438202817122 1103 84-85 0.3583892833820457 1103 86-87 0.3469026204340615 1103 88-89 0.46066254973822396 1103 90-91 0.3533711253382812 1103 92-93 0.2863093567531152 1103 94-95 0.22696729211725142 1103 96-97 0.13523217234596174 1103 98-99 0.13443911674283626 1103 100-101 0.15729856621766203 1103 102-103 -0.10540633801001675 1103 104-105 0.11540565403578995 1103 106-107 0.10458464493878594 1103 108-109 0.0791919621884496 1103 110-111 0.16291033044594272 1103 112-113 0.2334978714228022 1103 114-115 0.252943721730972 1103 116-117 0.28006126568835654 1103 118-119 0.21086899119337943 1103 120-121 0.0836046260464478 1103 122-123 -0.009527734352186457 1103 124-125 -0.15483040329459996 1104 1 0.20911160918828386 1104 2 0.242544811911376 1104 3 0.20123537582690787 1104 4 0.28899938833053085 1104 5 0.22473910427883226 1104 6 0.21831133894235677 1104 7 0.20848783319981834 1104 8 0.26948852402216517 1104 9 0.433796484795522 1104 10-11 0.4290097320170645 1104 12-13 0.37255425285579946 1104 14-15 0.31410302336540497 1104 16-17 0.29461039000602085 1104 18-19 0.2404732225704791 1104 20-21 0.24733147610719897 1104 22-23 0.38149396858737816 1104 24-25 0.33450185093741425 1104 26-27 0.4010867842696797 1104 28-29 0.28816301123707433 1104 30-31 0.25104992823362693 1104 32-33 0.2484241245770562 1104 34-35 0.20558035998958957 1104 36-37 0.14235867877214758 1104 38-39 0.06468535738069647 1104 40-41 0.07117841416394555 1104 42-43 -0.018846757742018383 1104 44-45 0.010562552486419463 1104 46-47 -3.262319466301733E-4 1104 48-49 -0.08023977083103517 1104 50-51 -0.055187476110234 1104 52-53 -0.19948010171883368 1104 54-55 -0.18037450670437494 1104 56-57 -0.17354657514335514 1104 58-59 -0.17884324423017262 1104 60-61 -0.24655765581184141 1104 62-63 -0.15215671802063468 1104 64-65 -0.07996299968607445 1104 66-67 -0.045995111723847515 1104 68-69 -0.11085900905272439 1104 70-71 -0.2221221587327875 1104 72-73 -0.19411771384847043 1104 74-75 -0.2624360408157642 1104 76-77 -0.22755710036610566 1104 78-79 -0.05690463975525262 1104 80-81 -0.04393901604867523 1104 82-83 0.006010220807254996 1104 84-85 -0.049375636522949407 1104 86-87 0.014636209801892619 1104 88-89 -0.03429525510285103 1104 90-91 -0.11577840217700341 1104 92-93 -0.018141600849588713 1104 94-95 0.04908058738229926 1104 96-97 -0.0882738158292895 1104 98-99 -0.14966013031360959 1104 100-101 -0.15376212406481926 1104 102-103 -0.23131062827228277 1104 104-105 -0.24312282137984909 1104 106-107 -0.20602124722352855 1104 108-109 -0.15766386680745725 1104 110-111 -0.24861627524408547 1104 112-113 -0.284506070160905 1104 114-115 -0.4318367118432356 1104 116-117 -0.4274277433570717 1104 118-119 -0.34691361530517995 1104 120-121 -0.4730035906395891 1104 122-123 -0.4371784493958977 1104 124-125 -0.5299246475717219 1105 1 0.28360797571217944 1105 2 0.37001994133462546 1105 3 0.32649920675510913 1105 4 0.33233764879955174 1105 5 0.25988447769842793 1105 6 0.4097276727409067 1105 7 0.37636859193288075 1105 8 0.494240712014161 1105 9 0.4071839020368344 1105 10-11 0.45348911711052864 1105 12-13 0.4766801216931569 1105 14-15 0.5334459038835178 1105 16-17 0.47873928387551246 1105 18-19 0.5253842247498781 1105 20-21 0.5645104961342824 1105 22-23 0.5513676734954913 1105 24-25 0.5401480734285258 1105 26-27 0.5191702889069205 1105 28-29 0.5356518908064203 1105 30-31 0.5172489065977715 1105 32-33 0.5271943115102999 1105 34-35 0.4335641073169043 1105 36-37 0.41837636146002666 1105 38-39 0.3209856055708329 1105 40-41 0.26274022450817114 1105 42-43 0.33447328378342434 1105 44-45 0.3436339913704529 1105 46-47 0.36676279337427076 1105 48-49 0.31267780391819855 1105 50-51 0.23618953227048678 1105 52-53 0.36429486339017103 1105 54-55 0.2708311748559922 1105 56-57 0.2512772511097907 1105 58-59 0.289931824004924 1105 60-61 0.24554151024179305 1105 62-63 0.3345977662457642 1105 64-65 0.25756513655037594 1105 66-67 0.1479717366739166 1105 68-69 0.1375905932243029 1105 70-71 0.16016229053948905 1105 72-73 -0.013216391771919689 1105 74-75 -0.0024310907631743817 1105 76-77 0.11919504637545231 1105 78-79 0.0815239935489771 1105 80-81 0.06933661250487688 1105 82-83 0.0943978099253826 1105 84-85 0.17202935340506897 1105 86-87 0.008510107212050144 1105 88-89 0.02468214653204015 1105 90-91 0.03517070165721847 1105 92-93 0.0932865580745954 1105 94-95 -0.052671181230241615 1105 96-97 -0.15205098096166836 1105 98-99 -0.10506441898417407 1105 100-101 -0.13650459070352383 1105 102-103 -0.28137463520235784 1105 104-105 -0.16118336327310345 1105 106-107 -0.23626703108498148 1105 108-109 -0.2200772924501031 1105 110-111 -0.2433472192603645 1105 112-113 -0.3411287592769803 1105 114-115 -0.29587361723535466 1105 116-117 -0.2922053691193476 1105 118-119 -0.20043385895776922 1105 120-121 -0.26543523522581225 1105 122-123 -0.19187199301480007 1105 124-125 -0.33684523360294705 1106 1 0.18072242102982372 1106 2 0.20316372105676805 1106 3 0.2129352648209739 1106 4 0.20978228660687392 1106 5 0.2064878222624742 1106 6 0.21767826462286877 1106 7 0.13405856032272112 1106 8 0.19359887617656568 1106 9 0.33933629370187646 1106 10-11 0.3501532974186219 1106 12-13 0.2197471648206033 1106 14-15 0.22976602842537375 1106 16-17 0.23124370949199147 1106 18-19 0.12175190194039232 1106 20-21 0.10316555288516582 1106 22-23 0.16777908357725835 1106 24-25 0.10874561451520748 1106 26-27 0.28049416241853464 1106 28-29 0.32893555804004393 1106 30-31 0.25913851505320196 1106 32-33 0.29839708816302846 1106 34-35 0.2547836370481349 1106 36-37 0.17759659240544323 1106 38-39 0.11637680175174125 1106 40-41 0.026044764384252517 1106 42-43 0.08338752606541533 1106 44-45 0.01716243398928441 1106 46-47 0.11384030419159075 1106 48-49 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0.4935730559820257 2205 84-85 0.4921750515949057 2205 86-87 0.47725558048096417 2205 88-89 0.6108304152466957 2205 90-91 0.541861967403662 2205 92-93 0.4872140882374403 2205 94-95 0.5013646217558865 2205 96-97 0.44163055840344256 2205 98-99 0.5090185541859746 2205 100-101 0.4434330688020154 2205 102-103 0.5083456358615912 2205 104-105 0.5447695350199737 2205 106-107 0.5337371038781065 2205 108-109 0.5865029613892219 2205 110-111 0.4713391424725266 2205 112-113 0.46749948647151385 2205 114-115 0.634872598746675 2205 116-117 0.6739614147485042 2205 118-119 0.6857076937340381 2205 120-121 0.7862527251838927 2205 122-123 0.8584595941525919 2205 124-125 0.9064725870344681 2206 1 0.1343340871322809 2206 2 0.12044880665081337 2206 3 0.046788556823123884 2206 4 0.1555915303657116 2206 5 0.1384946525280526 2206 6 0.13541720775708654 2206 7 0.07434103063529562 2206 8 0.17244430242097764 2206 9 0.154409121104365 2206 10-11 0.13247910020162124 2206 12-13 -0.008707186127153932 2206 14-15 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0.8705801151754784 2208 80-81 0.835801753986793 2208 82-83 0.8389476075855029 2208 84-85 0.7752740458182856 2208 86-87 0.8040792225847824 2208 88-89 0.8047229369212303 2208 90-91 0.650991174022824 2208 92-93 0.6882660484755405 2208 94-95 0.7356898033137185 2208 96-97 0.8306471649580978 2208 98-99 0.792283711698996 2208 100-101 0.8477039540613767 2208 102-103 0.8388483286843176 2208 104-105 0.7085596219136008 2208 106-107 0.7957988482016738 2208 108-109 0.8309069238949682 2208 110-111 0.8776824260546157 2208 112-113 0.8850678333422692 2208 114-115 0.7359404422404019 2208 116-117 0.8398202345981751 2208 118-119 0.8272059978910526 2208 120-121 0.9119188444891329 2208 122-123 0.9892621139700104 2208 124-125 1.1716388969387772 2209 1 0.17290693954099012 2209 2 0.1243899612975099 2209 3 0.08605547376175693 2209 4 0.07836565048368271 2209 5 0.03930159097247099 2209 6 0.17990100610579418 2209 7 0.189732599362614 2209 8 0.02964465204714628 2209 9 0.2364109195411075 2209 10-11 0.1636062830070557 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110-111 -0.5355794249611847 2216 112-113 -0.46444743804810074 2216 114-115 -0.5374542545713865 2216 116-117 -0.6721376609789509 2216 118-119 -0.5552792451606514 2216 120-121 -0.36560480432432385 2216 122-123 -0.38803535772458986 2216 124-125 -0.2112368342563684 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 23813.0 3 4308.0 4 2769.0 5 2215.0 6 2240.0 7 2532.0 8 2878.0 9 3221.0 10 3605.0 11 3943.0 12 4314.0 13 4471.0 14 4368.0 15 4300.0 16 3945.0 17 3624.0 18 3470.0 19 3304.0 20 3266.0 21 3337.0 22 3916.0 23 5364.0 24 8065.0 25 11626.0 26 14192.0 27 13991.0 28 16272.0 29 19990.0 30 23371.0 31 28577.0 32 36902.0 33 49160.0 34 69671.0 35 107219.0 36 207545.0 37 396956.0 38 171733.0 39 532.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.82396059818323 18.349276045757453 12.696289170542165 24.130474185517148 2 15.522009914658636 19.408022715863453 40.10769641064257 24.96227095883534 3 19.15620882223756 24.408373596034387 28.653526385141493 27.78189119658656 4 12.391522605339942 15.906723559125279 36.98302638596932 34.71872744956546 5 13.463253758249794 38.688223143899606 34.148826927135104 13.6996961707155 6 32.93875450334534 36.25169463866538 17.27911640284699 13.530434455142288 7 29.638103455037452 32.29781560061179 20.122671477312835 17.94140946703792 8 26.757092526768545 35.99596550285332 19.121900794036726 18.12504117634141 9 26.602170187567893 16.595778055772332 19.13200340390822 37.67004835275156 10-11 25.20940155019304 26.967358887531596 27.153476169654567 20.669763392620798 12-13 25.41734657422669 24.127100654554408 27.515403068025268 22.940149703193637 14-15 22.514418644414093 25.770853848063354 24.656930968938397 27.05779653858416 16-17 22.867955160872235 29.267040652861464 25.47206770182804 22.392936484438266 18-19 22.91695669855024 28.104432654498595 26.66314249333433 22.315468153616834 20-21 23.51952483304961 27.272844919370876 26.696925383400604 22.51070486417891 22-23 23.303770027388147 27.277090895401113 26.532701073878982 22.886438003331754 24-25 23.08006635268097 27.379500472547168 26.62138579848706 22.919047376284798 26-27 22.83952723304114 27.72703939476292 26.45625956614271 22.977173806053234 28-29 22.83587339769671 27.560420564540955 26.6837934573359 22.919912580426438 30-31 22.899798235689733 27.217358397336476 26.773045543047957 23.109797823925835 32-33 22.716082013989 27.44113342567306 26.690154211522803 23.15263034881514 34-35 23.341772608590965 27.44501725894536 26.349205602235624 22.86400453022806 36-37 23.356584231663497 27.163315894179195 26.29116210962393 23.188937764533378 38-39 22.853143990136502 27.373247456478566 26.567838218546765 23.205770334838167 40-41 23.11980097223682 27.1322778545535 26.764826297766742 22.98309487544294 42-43 23.066361932967475 27.038815115063375 26.70230346362686 23.19251948834229 44-45 22.85022623831063 27.278010765633738 26.742238916126727 23.12952407992891 46-47 23.10273901423723 27.5297107260574 26.30453665777126 23.063013601934113 48-49 23.011399955294294 27.196756091152587 26.515072489911805 23.276771463641317 50-51 22.97434127709303 27.254638217105033 26.660562115442684 23.110458390359252 52-53 23.142018814324235 27.161160985297094 26.56711524675565 23.12970495362302 54-55 22.838472250708943 27.13887998883103 26.701493889418536 23.321153871041496 56-57 22.806778810237628 27.52814210168215 26.63688894727657 23.028190140803645 58-59 22.97859040352505 27.021252732376517 26.639720028957115 23.360436835141325 60-61 22.826252773079453 27.102785412964813 26.989764165205532 23.081197648750198 62-63 22.742012587544767 27.254438366403516 26.83541563643023 23.168133409621483 64-65 22.722541793009114 27.15176936941459 26.713329056121925 23.412359781454366 66-67 22.576328775417924 27.389532938837764 26.548958537179356 23.485179748564956 68-69 22.60649812698831 27.045175034040497 26.89736554787945 23.450961291091744 70-71 22.5921848878236 27.049005140086674 26.865433417112524 23.493376554977203 72-73 22.621162529677378 27.05921985064641 26.76481868857165 23.55479893110456 74-75 22.457103842173357 27.233731656899757 26.75073933199134 23.558425168935546 76-77 22.65011480549733 27.0619008496195 26.79695603665148 23.49102830823168 78-79 22.733474330833783 27.027401176582703 26.90414274604724 23.334981746536272 80-81 22.63532287015036 27.32634315010018 26.761196365272045 23.277137614477418 82-83 22.624585929261603 27.23253098937367 26.84307802353502 23.299805057829705 84-85 22.73022817326486 27.02875146274969 26.818907667161305 23.422112696824147 86-87 22.629611314099762 26.842123632963926 27.098912881071048 23.429352171865265 88-89 22.71383341824039 27.03899488871293 26.915967899250425 23.331203793796256 90-91 22.682349305645968 27.223611331155027 26.886512763157278 23.207526600041724 92-93 22.702456172274022 26.788795537540626 27.056130548270108 23.452617741915244 94-95 22.53190330399836 26.67763672606473 27.143955754790937 23.646504215145978 96-97 22.657681063370465 26.741986780418074 27.021084272383778 23.579247883827676 98-99 22.548863897951012 26.72095705435823 26.950604923703654 23.779574123987103 100-101 22.386712303840675 26.654099188695703 27.195164802068106 23.764023705395516 102-103 22.41881820705926 26.45441725268985 27.281019750929037 23.845744789321856 104-105 22.277181323596253 26.63455395872198 27.22326515101901 23.86499956666276 106-107 22.33803486380151 26.596394708934945 27.16502575880223 23.90054466846131 108-109 22.412984431827184 26.6944084306099 26.89539307891226 23.997214058650655 110-111 22.30009306068474 26.89128292369769 27.100149846134176 23.70847416948339 112-113 22.274533211028892 26.909696418045893 27.222098253240002 23.593672117685212 114-115 22.168791310151935 26.88506061763555 27.03832044795166 23.907827624260857 116-117 22.118507973670123 26.85388565680571 27.22161763638966 23.805988733134505 118-119 21.998913996188 27.054128344621027 26.92084962535223 24.02610803383875 120-121 22.015090014915874 27.11904026971725 27.114176824664572 23.75169289070231 122-123 22.029671421304926 27.149205388058405 27.1063795242172 23.714743666419462 124-125 21.996907345466298 27.15496567922121 27.02292195851388 23.825205016798613 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 597.0 1 520.5 2 385.5 3 296.0 4 235.5 5 187.5 6 166.5 7 165.5 8 179.5 9 196.5 10 174.5 11 156.5 12 161.0 13 174.0 14 185.0 15 187.5 16 231.0 17 300.5 18 397.5 19 511.0 20 687.0 21 1013.5 22 1436.0 23 2023.5 24 2697.0 25 3505.0 26 4849.0 27 6575.0 28 8213.0 29 10241.0 30 12837.5 31 15255.5 32 18073.0 33 20756.5 34 22973.0 35 25291.5 36 28695.0 37 32258.0 38 34508.5 39 36450.0 40 37796.0 41 39989.5 42 42453.0 43 44182.0 44 46687.0 45 49713.5 46 50164.5 47 49183.5 48 48916.5 49 48073.5 50 47374.5 51 46326.0 52 45751.0 53 45567.5 54 45412.0 55 43339.5 56 39336.0 57 36008.0 58 33040.5 59 29184.5 60 24125.0 61 19602.0 62 16210.5 63 12942.0 64 9968.5 65 7760.0 66 5792.0 67 4792.0 68 3674.0 69 2313.0 70 1625.5 71 1165.0 72 867.5 73 646.5 74 485.0 75 386.0 76 274.0 77 145.5 78 79.0 79 53.5 80 37.5 81 23.0 82 16.0 83 14.5 84 13.0 85 9.0 86 5.0 87 7.0 88 6.5 89 4.5 90 4.5 91 3.0 92 1.5 93 2.0 94 2.0 95 0.5 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.042823361477013815 2 0.009803883122026973 3 0.00352939792392971 4 0.006666640522978342 5 0.021646973933435554 6 0.031137132795557666 7 0.004313708573691868 8 7.843106497621577E-5 9 0.0 10-11 1.9607766244053945E-4 12-13 0.0012156815071313447 14-15 5.098019223454026E-4 16-17 1.9607766244053945E-4 18-19 1.1764659746432367E-4 20-21 3.9215532488107884E-5 22-23 7.843106497621577E-5 24-25 0.0 26-27 3.9215532488107884E-5 28-29 3.52939792392971E-4 30-31 1.9607766244053945E-4 32-33 2.3529319492864733E-4 34-35 0.0010980349096670209 36-37 3.9215532488107884E-5 38-39 7.843106497621577E-5 40-41 5.490174548335104E-4 42-43 9.411727797145893E-4 44-45 0.0017254834294767472 46-47 1.9607766244053945E-4 48-49 0.0 50-51 0.0 52-53 0.0014901902345480999 54-55 0.003490182391441602 56-57 1.9607766244053945E-4 58-59 0.0012548970396194523 60-61 0.0018431300269410708 62-63 0.001137250442155129 64-65 0.0024313630142626893 66-67 0.003411751326465386 68-69 0.004156846443739436 70-71 0.002078423221869718 72-73 0.0034509668589534945 74-75 0.0028627338716318757 76-77 0.001764698961964855 78-79 0.003254889196512955 80-81 0.002078423221869718 82-83 9.019572472264815E-4 84-85 7.05879584785942E-4 86-87 0.0023137164167983657 88-89 0.006392131795561586 90-91 5.490174548335104E-4 92-93 0.0010196038446908052 94-95 0.0015294057670362077 96-97 1.9607766244053945E-4 98-99 7.450951172740499E-4 100-101 0.0016470523645005313 102-103 0.002117638754357826 104-105 0.0012941125721075604 106-107 0.007921537562597794 108-109 0.0025097940792389046 110-111 0.0023921474817745815 112-113 0.003490182391441602 114-115 0.003607828988905926 116-117 0.002156854286845934 118-119 0.047686087505539196 120-121 0.014784255748016674 122-123 0.005843114340728076 124-125 0.003803906651346465 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1275005.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.07566593738075 #Duplication Level Percentage of deduplicated Percentage of total 1 87.1613646414597 66.30858859109918 2 8.489428232156056 12.916778123777462 3 2.113069479295925 4.822595034281757 4 0.8283398521355105 2.5206602349472185 5 0.40206581032739463 1.5293712135654582 6 0.2267174768279783 1.0348609817598684 7 0.14697041879588155 0.7826610738094699 8 0.10576519281741383 0.6436925981264189 9 0.07580808141107369 0.5190435249104344 >10 0.40734850091497304 5.811352141317339 >50 0.03147785786650116 1.672258555734214 >100 0.011462511367402473 1.3238066538290294 >500 1.8194462427526814E-4 0.11433127284216199 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.921553248810789E-5 0.0 18-19 0.0 0.0 0.0 7.843106497621578E-5 0.0 20-21 0.0 0.0 0.0 1.1764659746432368E-4 0.0 22-23 0.0 0.0 0.0 1.5686212995243156E-4 0.0 24-25 0.0 0.0 0.0 2.3529319492864733E-4 3.921553248810789E-5 26-27 0.0 0.0 0.0 6.274485198097263E-4 7.843106497621578E-5 28-29 7.843106497621578E-5 0.0 0.0 0.0022352853518221496 7.843106497621578E-5 30-31 1.5686212995243156E-4 0.0 0.0 0.009568589927098326 7.843106497621578E-5 32-33 1.5686212995243156E-4 0.0 0.0 0.023529319492864735 7.843106497621578E-5 34-35 1.5686212995243156E-4 0.0 0.0 0.04266649934706139 7.843106497621578E-5 36-37 1.5686212995243156E-4 0.0 0.0 0.07529382237716714 7.843106497621578E-5 38-39 1.5686212995243156E-4 7.843106497621578E-5 0.0 0.13392104344688843 7.843106497621578E-5 40-41 1.5686212995243156E-4 7.843106497621578E-5 0.0 0.2018031301838032 7.843106497621578E-5 42-43 2.3529319492864733E-4 7.843106497621578E-5 0.0 0.26462641322975206 7.843106497621578E-5 44-45 2.3529319492864733E-4 7.843106497621578E-5 0.0 0.33497907851341757 7.843106497621578E-5 46-47 2.3529319492864733E-4 7.843106497621578E-5 0.0 0.4236453974690295 7.843106497621578E-5 48-49 2.3529319492864733E-4 7.843106497621578E-5 0.0 0.5247038246908836 7.843106497621578E-5 50-51 2.3529319492864733E-4 7.843106497621578E-5 0.0 0.6371347563342888 1.1764659746432368E-4 52-53 2.3529319492864733E-4 7.843106497621578E-5 0.0 0.7441147289618473 1.5686212995243156E-4 54-55 2.3529319492864733E-4 7.843106497621578E-5 0.0 0.8557221344230023 1.5686212995243156E-4 56-57 2.3529319492864733E-4 7.843106497621578E-5 0.0 0.9956039388080831 1.5686212995243156E-4 58-59 2.3529319492864733E-4 7.843106497621578E-5 0.0 1.154701354112337 1.5686212995243156E-4 60-61 2.3529319492864733E-4 7.843106497621578E-5 0.0 1.3272104815275236 1.5686212995243156E-4 62-63 2.3529319492864733E-4 7.843106497621578E-5 0.0 1.4969745216685424 1.5686212995243156E-4 64-65 2.3529319492864733E-4 7.843106497621578E-5 0.0 1.666856208407026 1.5686212995243156E-4 66-67 2.3529319492864733E-4 7.843106497621578E-5 0.0 1.8676397347461382 1.5686212995243156E-4 68-69 2.3529319492864733E-4 7.843106497621578E-5 0.0 2.096815306606641 1.5686212995243156E-4 70-71 2.3529319492864733E-4 7.843106497621578E-5 0.0 2.3472456970756976 1.5686212995243156E-4 72-73 2.3529319492864733E-4 7.843106497621578E-5 0.0 2.6001466660915056 3.1372425990486313E-4 74-75 2.3529319492864733E-4 7.843106497621578E-5 0.0 2.8497927459108006 3.1372425990486313E-4 76-77 2.3529319492864733E-4 7.843106497621578E-5 0.0 3.1162622891674934 3.1372425990486313E-4 78-79 2.3529319492864733E-4 7.843106497621578E-5 0.0 3.4208101144701395 3.1372425990486313E-4 80-81 2.3529319492864733E-4 7.843106497621578E-5 0.0 3.7534362610342704 3.1372425990486313E-4 82-83 2.3529319492864733E-4 7.843106497621578E-5 0.0 4.105670173842455 3.1372425990486313E-4 84-85 2.3529319492864733E-4 7.843106497621578E-5 0.0 4.449276669503257 3.1372425990486313E-4 86-87 2.3529319492864733E-4 7.843106497621578E-5 0.0 4.793275320488939 3.1372425990486313E-4 88-89 2.3529319492864733E-4 7.843106497621578E-5 0.0 5.187156128799495 3.1372425990486313E-4 90-91 3.1372425990486313E-4 7.843106497621578E-5 0.0 5.607625068136988 3.1372425990486313E-4 92-93 3.1372425990486313E-4 7.843106497621578E-5 0.0 6.062838969258944 3.1372425990486313E-4 94-95 3.1372425990486313E-4 7.843106497621578E-5 0.0 6.513543084144768 3.1372425990486313E-4 96-97 3.1372425990486313E-4 7.843106497621578E-5 0.0 6.954992333363398 3.1372425990486313E-4 98-99 3.1372425990486313E-4 7.843106497621578E-5 0.0 7.434049278238125 3.1372425990486313E-4 100-101 3.1372425990486313E-4 7.843106497621578E-5 0.0 7.972556970364822 3.1372425990486313E-4 102-103 3.5293979239297104E-4 7.843106497621578E-5 0.0 8.529221454033515 3.1372425990486313E-4 104-105 4.7058638985729466E-4 7.843106497621578E-5 0.0 9.080983996141192 3.1372425990486313E-4 106-107 4.7058638985729466E-4 7.843106497621578E-5 0.0 9.612354461355054 3.1372425990486313E-4 108-109 4.7058638985729466E-4 7.843106497621578E-5 0.0 10.169175807153698 3.1372425990486313E-4 110-111 4.7058638985729466E-4 7.843106497621578E-5 0.0 10.780193018850905 3.1372425990486313E-4 112-113 4.7058638985729466E-4 7.843106497621578E-5 0.0 11.41952384500453 3.1372425990486313E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 2095 0.0 46.32172 1 GGTATCA 1355 0.0 43.498775 1 ATCAACG 2270 0.0 41.686844 3 TCAACGC 2290 0.0 41.322765 4 CAACGCA 2360 0.0 40.09709 5 AACGCAG 2460 0.0 38.461098 6 GTACATG 6145 0.0 36.428986 1 TATCAAC 2635 0.0 36.138252 2 TACATGG 6515 0.0 34.347977 2 ACATGGG 6625 0.0 33.059 3 ACGCAGA 3125 0.0 30.271824 7 CGCAGAG 3235 0.0 29.057432 8 CATGGGA 4260 0.0 28.360521 4 ATAGCGA 155 6.8268782E-6 26.873608 6 TGCGCGA 45 0.009532002 26.44393 52-53 GAGTACA 4665 0.0 25.779879 1 CATGGGG 2930 0.0 25.187311 4 AGTACAT 4705 0.0 24.66623 2 ATGGGGA 1725 0.0 23.806072 5 GCAGAGT 3920 0.0 23.220942 9 >>END_MODULE