FastQCFastQC Report
Thu 26 May 2016
SRR1364392_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364392_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1275005
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32480.25474409904274886No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31410.24635197509029375No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16930.1327837930047333No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25250.069.339261
GTATCAA41850.053.503921
ATCAACG50400.043.350333
TCAACGC51300.042.58984
CAACGCA52000.041.901995
TATCAAC53300.041.2150732
AACGCAG53750.040.6293686
ACGCAGA62850.034.8372547
GTACATG59500.034.229651
CGCAGAG63700.034.1855858
GTGGTAT10950.033.174851
ACATGGG60700.032.659723
TACATGG62350.032.6547662
TGGTATC11700.031.0385112
GCAGAGT72600.029.8308859
GAGTACT42400.028.76665712-13
CATGGGA40450.028.1107064
TATAACG1505.272268E-627.7820132
GTACTTT44300.027.19710514-15
CAGAGTA71150.027.09389910-11
Produced by FastQC (version 0.11.5)