##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364392_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1275005 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.27928596358446 33.0 31.0 34.0 30.0 34.0 2 32.28370320116392 33.0 31.0 34.0 30.0 34.0 3 32.40084940843369 34.0 31.0 34.0 30.0 34.0 4 36.01218269732276 37.0 35.0 37.0 35.0 37.0 5 35.94926372837754 37.0 35.0 37.0 35.0 37.0 6 36.016187387500445 37.0 35.0 37.0 35.0 37.0 7 35.975979702040384 37.0 35.0 37.0 35.0 37.0 8 35.98337104560374 37.0 35.0 37.0 35.0 37.0 9 37.72741675522841 39.0 38.0 39.0 35.0 39.0 10-11 37.685956525660686 39.0 37.5 39.0 35.0 39.0 12-13 37.65349155493507 39.0 37.0 39.0 35.0 39.0 14-15 39.053443711985445 41.0 38.0 41.0 35.5 41.0 16-17 39.06510248979416 41.0 38.0 41.0 35.5 41.0 18-19 39.12528225379508 41.0 38.5 41.0 36.0 41.0 20-21 39.13637515146999 41.0 39.0 41.0 35.0 41.0 22-23 39.10212705048215 41.0 39.0 41.0 35.0 41.0 24-25 39.072811871325996 41.0 39.0 41.0 35.0 41.0 26-27 38.98157928792436 41.0 39.0 41.0 35.0 41.0 28-29 38.89213689358081 40.5 38.0 41.0 35.0 41.0 30-31 38.78340320234038 40.0 38.0 41.0 35.0 41.0 32-33 38.65792094932961 40.0 38.0 41.0 34.5 41.0 34-35 38.56177936557111 40.0 38.0 41.0 34.0 41.0 36-37 38.45490605919193 40.0 38.0 41.0 34.0 41.0 38-39 38.37216481503994 40.0 38.0 41.0 34.0 41.0 40-41 38.26132642617088 40.0 38.0 41.0 34.0 41.0 42-43 38.11155407233697 40.0 38.0 41.0 33.0 41.0 44-45 37.94079513413673 40.0 38.0 41.0 33.0 41.0 46-47 37.700572154618996 40.0 37.0 41.0 33.0 41.0 48-49 37.47511460739369 40.0 37.0 41.0 32.5 41.0 50-51 37.31106466249152 40.0 37.0 41.0 32.0 41.0 52-53 37.16612679950275 40.0 36.5 41.0 31.5 41.0 54-55 36.98448594319238 40.0 36.0 41.0 31.0 41.0 56-57 36.79317649734707 39.5 36.0 41.0 31.0 41.0 58-59 36.57306794875314 39.0 35.0 41.0 31.0 41.0 60-61 36.32352971164819 39.0 35.0 41.0 31.0 41.0 62-63 36.08272242069639 38.5 35.0 41.0 30.0 41.0 64-65 35.80113450535488 38.0 35.0 40.0 30.0 41.0 66-67 35.47293304732138 37.0 35.0 40.0 29.5 41.0 68-69 35.14130572036973 37.0 34.5 39.5 29.0 41.0 70-71 34.88304438021812 36.0 34.0 39.0 29.5 41.0 72-73 34.68154477825577 36.0 34.0 39.0 29.0 41.0 74-75 34.34339904549394 35.5 34.0 38.5 29.0 40.0 76-77 34.01359759373493 35.0 34.0 37.0 29.0 39.0 78-79 33.69774314610531 35.0 34.0 37.0 29.0 39.0 80-81 33.39731844188846 35.0 34.0 36.0 29.0 39.0 82-83 33.12941988462791 35.0 34.0 36.0 29.0 37.0 84-85 32.89634079866353 35.0 34.0 36.0 29.0 37.0 86-87 32.708307026246956 35.0 34.0 35.0 28.5 36.5 88-89 32.552707244285315 35.0 34.0 35.0 28.0 36.0 90-91 32.416185426723814 35.0 33.5 35.0 27.5 36.0 92-93 32.29312159560159 35.0 33.0 35.0 27.0 36.0 94-95 32.205845859428 35.0 33.0 35.0 27.0 35.5 96-97 32.12915204254101 35.0 33.0 35.0 27.0 35.0 98-99 32.0591123172066 35.0 33.0 35.0 27.0 35.0 100-101 32.000401174897355 35.0 33.0 35.0 27.0 35.0 102-103 31.02079325179117 34.0 31.5 35.0 24.5 35.0 104-105 31.742802577244795 35.0 33.0 35.0 25.0 35.0 106-107 32.038968474633435 35.0 33.0 35.0 27.0 35.0 108-109 32.05876016172486 35.0 34.0 35.0 27.0 35.0 110-111 32.03475084411434 35.0 34.0 35.0 27.0 35.0 112-113 31.97626440680625 35.0 33.5 35.0 27.0 35.0 114-115 31.904319590903565 35.0 33.0 35.0 27.0 35.0 116-117 31.852246853933906 35.0 33.0 35.0 27.0 35.0 118-119 31.752370775016566 35.0 33.0 35.0 25.5 35.0 120-121 31.62797126285779 35.0 33.0 35.0 25.0 35.0 122-123 31.490728271653836 35.0 33.0 35.0 25.0 35.0 124-125 30.806213308967415 34.5 32.0 35.0 22.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.027980951200291315 1101 2 0.08005311303492846 1101 3 0.10371746461404996 1101 4 0.07875336570855751 1101 5 0.15711585764698555 1101 6 0.16235092346939695 1101 7 0.22084270451559718 1101 8 0.182541313751301 1101 9 0.10412798999893624 1101 10-11 0.21684603017611437 1101 12-13 0.1950705620922406 1101 14-15 0.22587642841812539 1101 16-17 0.11763730686750762 1101 18-19 0.25859816962412197 1101 20-21 0.1422081120946146 1101 22-23 0.21574149431280176 1101 24-25 0.11948362330513618 1101 26-27 0.09910781231930343 1101 28-29 0.10883205304425303 1101 30-31 0.186235152209683 1101 32-33 0.05455596482073588 1101 34-35 0.11917213847570451 1101 36-37 0.19306728302732523 1101 38-39 0.19828455654496935 1101 40-41 0.10083525135324578 1101 42-43 0.028289171800281565 1101 44-45 0.040543431471370184 1101 46-47 0.06608669148825896 1101 48-49 -0.019431663063542715 1101 50-51 -0.04314516526382306 1101 52-53 0.022053502913898626 1101 54-55 -0.005687463635950962 1101 56-57 -0.03287668549080536 1101 58-59 -0.18707064496071268 1101 60-61 -0.12448732164590126 1101 62-63 -0.288841728907947 1101 64-65 -0.22694025889428104 1101 66-67 -0.19525578934862153 1101 68-69 -0.20513995547921127 1101 70-71 -0.06314095431572753 1101 72-73 -0.1501615434764929 1101 74-75 -0.07786024488585497 1101 76-77 -0.014672266657925093 1101 78-79 -0.2770332021002133 1101 80-81 -0.2342453186729614 1101 82-83 -0.21147792933535214 1101 84-85 -0.31652531148516516 1101 86-87 -0.31874825624944236 1101 88-89 -0.2990570488481339 1101 90-91 -0.8546557667860313 1101 92-93 -0.8105305619466492 1101 94-95 -0.576138591038454 1101 96-97 -0.4040944978458043 1101 98-99 -0.3863829355255888 1101 100-101 -0.5299008406310612 1101 102-103 -0.4183290097572332 1101 104-105 -0.48372413984132123 1101 106-107 -0.3426279154341785 1101 108-109 -0.13584182802646438 1101 110-111 -0.3137655666510355 1101 112-113 -0.2412893617707752 1101 114-115 -0.3787732849657637 1101 116-117 -0.3645976413226286 1101 118-119 -0.30260576643330594 1101 120-121 -0.28363447871920044 1101 122-123 -0.23928693606021767 1101 124-125 -0.2999883774653789 1102 1 0.12399706264363886 1102 2 0.1682063945395953 1102 3 0.06148472813112704 1102 4 0.10419279535877024 1102 5 0.17177973353962273 1102 6 0.04996950205079287 1102 7 0.14323925239848023 1102 8 0.15176540821548912 1102 9 0.2453810240862424 1102 10-11 0.2573729353484211 1102 12-13 0.24895096921692073 1102 14-15 0.2890991861250072 1102 16-17 0.3429562524708629 1102 18-19 0.3632328479229159 1102 20-21 0.3182206471258695 1102 22-23 0.27534152245505794 1102 24-25 0.32628848019002277 1102 26-27 0.4265201315932359 1102 28-29 0.3718246891531152 1102 30-31 0.4134012267163172 1102 32-33 0.3287190257604564 1102 34-35 0.28996164610342845 1102 36-37 0.3875012718407831 1102 38-39 0.38383497222958596 1102 40-41 0.31731430295911167 1102 42-43 0.32785677771484245 1102 44-45 0.2571113070825888 1102 46-47 0.29120483034686373 1102 48-49 0.16330188837230253 1102 50-51 0.09940856886218086 1102 52-53 0.022776407195046033 1102 54-55 0.1115731219575622 1102 56-57 0.14800709870496576 1102 58-59 0.007986870781358846 1102 60-61 0.003386999715246475 1102 62-63 -0.03531515206198321 1102 64-65 0.07278418148516863 1102 66-67 -0.12342325924227282 1102 68-69 -0.2021806210436381 1102 70-71 -0.007353909135673575 1102 72-73 0.01304128013017447 1102 74-75 0.028235778847452764 1102 76-77 0.008331391835760371 1102 78-79 -0.07053497858032642 1102 80-81 -0.10389037442980964 1102 82-83 -0.07977889907069624 1102 84-85 -0.05369918374275784 1102 86-87 -0.0775078275490344 1102 88-89 -0.0872613381974574 1102 90-91 -0.05739436037662671 1102 92-93 -0.07207240596817854 1102 94-95 0.02504543588430863 1102 96-97 -0.11813983033486863 1102 98-99 -0.17391356954723491 1102 100-101 -0.09833226146336926 1102 102-103 -0.025211551003231136 1102 104-105 -0.159624692133157 1102 106-107 -0.22555377230734308 1102 108-109 -0.28814890697722717 1102 110-111 -0.21151272017878853 1102 112-113 -0.2910850372436258 1102 114-115 -0.30424320206335764 1102 116-117 -0.33437021669111644 1102 118-119 -0.4198393823618254 1102 120-121 -0.4406099480500032 1102 122-123 -0.5674778578364617 1102 124-125 -0.6633673833400735 1103 1 0.18028151244399027 1103 2 0.16048369872470403 1103 3 0.1964239861582726 1103 4 0.17337881473340389 1103 5 0.10974993751214868 1103 6 0.1459409705086756 1103 7 0.18599837642412353 1103 8 0.171808349798809 1103 9 0.331894311790613 1103 10-11 0.31351099338745314 1103 12-13 0.2753142159740207 1103 14-15 0.3559376332690931 1103 16-17 0.3051203470272128 1103 18-19 0.2963413602855951 1103 20-21 0.25383479238892903 1103 22-23 0.3064370420185085 1103 24-25 0.19928407995070785 1103 26-27 0.12957186941196142 1103 28-29 0.16540275839429341 1103 30-31 0.23382521120303323 1103 32-33 0.168031790697718 1103 34-35 0.3444448407535532 1103 36-37 0.36025649208612975 1103 38-39 0.23130006097694178 1103 40-41 0.2898372058440941 1103 42-43 0.2473922278588958 1103 44-45 0.16492480822189748 1103 46-47 0.2727477995112366 1103 48-49 0.13118905214861343 1103 50-51 -0.023432152658251937 1103 52-53 0.06207304782235923 1103 54-55 0.13362948066405522 1103 56-57 0.22031295989162913 1103 58-59 0.1476637056641863 1103 60-61 0.09477576955769251 1103 62-63 0.15123250588199255 1103 64-65 0.0630257915399497 1103 66-67 0.05042968705105011 1103 68-69 1.4658363750186254E-4 1103 70-71 0.027285776185685506 1103 72-73 0.14115740910768437 1103 74-75 0.15538657084809415 1103 76-77 0.1651180290260541 1103 78-79 0.1625546513667615 1103 80-81 0.1547064287135811 1103 82-83 0.14643880457297342 1103 84-85 0.23846058270934378 1103 86-87 0.20208664605589632 1103 88-89 0.22360368859859392 1103 90-91 0.2573150413690328 1103 92-93 0.1528415623517958 1103 94-95 0.20895273807509085 1103 96-97 0.10198564225592577 1103 98-99 0.21003383125643182 1103 100-101 0.2162466606689435 1103 102-103 0.31595975281695843 1103 104-105 0.22927456911885358 1103 106-107 0.22059209329229645 1103 108-109 0.17972446627084082 1103 110-111 0.16491778192374085 1103 112-113 0.22155663391380642 1103 114-115 0.18947998596730642 1103 116-117 0.12003405370507281 1103 118-119 0.09491436123618868 1103 120-121 0.15069926105259412 1103 122-123 0.18704967866600342 1103 124-125 -0.053380494950001633 1104 1 0.10733997926899264 1104 2 0.067490621762758 1104 3 0.14742504735389161 1104 4 0.061278152749473236 1104 5 0.07076060000140671 1104 6 0.1378152440950089 1104 7 0.14853716059715083 1104 8 0.1625971718716812 1104 9 0.19496167244552254 1104 10-11 0.21940921967478744 1104 12-13 0.1762177482394236 1104 14-15 0.30607053538643214 1104 16-17 0.2994101039628845 1104 18-19 0.15859816962411344 1104 20-21 0.15232890527992282 1104 22-23 0.16716168702331657 1104 24-25 0.20963932781568673 1104 26-27 0.11173872978893229 1104 28-29 0.2403534885979468 1104 30-31 0.1937382942289574 1104 32-33 0.04077018071237859 1104 34-35 0.061992973554602315 1104 36-37 0.16658424993139676 1104 38-39 0.06360993009614191 1104 40-41 0.05690982194395389 1104 42-43 0.0632151597907793 1104 44-45 0.12874410176881668 1104 46-47 0.20197413737570713 1104 48-49 0.0676679040360213 1104 50-51 0.05186321345423295 1104 52-53 0.02604596206764853 1104 54-55 -0.01017566360802391 1104 56-57 0.11278453500912633 1104 58-59 0.09768846689517119 1104 60-61 0.0992509830601449 1104 62-63 0.013537827020002169 1104 64-65 -0.02901119870715263 1104 66-67 -0.0028413220954490725 1104 68-69 0.06443482457943617 1104 70-71 0.08904938222945447 1104 72-73 0.1873527701668607 1104 74-75 0.13580195313118537 1104 76-77 0.1557320064882859 1104 78-79 0.20242427590564205 1104 80-81 0.1346235543894636 1104 82-83 0.15522853624208466 1104 84-85 0.23032778165825363 1104 86-87 0.28016739798878376 1104 88-89 0.29401654422546386 1104 90-91 0.27459433794794563 1104 92-93 0.15799897303450194 1104 94-95 0.17221010327798325 1104 96-97 0.2616965927839985 1104 98-99 0.09300821096233136 1104 100-101 0.159064387689007 1104 102-103 0.10559641965206623 1104 104-105 0.14056366912390672 1104 106-107 0.26488639820916404 1104 108-109 0.29692594183808296 1104 110-111 0.26645297824492076 1104 112-113 0.22666553279702129 1104 114-115 0.1350083097835686 1104 116-117 0.06145807715244089 1104 118-119 0.04571430059644044 1104 120-121 -0.02585134787155141 1104 122-123 0.02850806463800737 1104 124-125 -0.12058955048590647 1105 1 0.23918930516808246 1105 2 0.1405140726369254 1105 3 0.180949828554688 1105 4 0.1391226798475742 1105 5 0.13317333813674992 1105 6 0.09699518538188556 1105 7 0.18890511450666025 1105 8 0.21002527579528163 1105 9 0.22133115262124647 1105 10-11 0.25624489855387367 1105 12-13 0.2605064188065498 1105 14-15 0.34985196306410415 1105 16-17 0.283948064678718 1105 18-19 0.2876611659184505 1105 20-21 0.2638556527516229 1105 22-23 0.1814256760781987 1105 24-25 0.26535601980553025 1105 26-27 0.28822391744404996 1105 28-29 0.19360367938121925 1105 30-31 0.2258968609137284 1105 32-33 0.20173117842297472 1105 34-35 0.1616119570063006 1105 36-37 0.2096783078057598 1105 38-39 0.22180769844742798 1105 40-41 0.23917350849561814 1105 42-43 0.07283242447421401 1105 44-45 -0.004797592111678739 1105 46-47 -0.04122758414258243 1105 48-49 -0.033742585950435 1105 50-51 -0.0628923433235613 1105 52-53 0.023673236857440827 1105 54-55 -0.05257066405489752 1105 56-57 -0.04800444837383111 1105 58-59 -0.10606174795086076 1105 60-61 -0.14204084316551047 1105 62-63 -0.13393812741202282 1105 64-65 -0.19673795580395392 1105 66-67 -0.06348042888595273 1105 68-69 -0.15365555513083962 1105 70-71 -0.2049251662501277 1105 72-73 -0.0953524335759326 1105 74-75 -0.08759928300695918 1105 76-77 -0.06401609083524562 1105 78-79 0.06123461326548352 1105 80-81 0.025628483816838354 1105 82-83 0.019432918413649247 1105 84-85 0.006230513124776849 1105 86-87 -0.06471912893055531 1105 88-89 0.00918364287588247 1105 90-91 -4.318217049998907E-4 1105 92-93 -0.03832987545951738 1105 94-95 -0.05495468534789438 1105 96-97 -0.13761823743607238 1105 98-99 -0.1257728385863217 1105 100-101 -0.08174769593938436 1105 102-103 -0.028874784104470308 1105 104-105 0.013526069349410363 1105 106-107 -0.0055795254912709424 1105 108-109 0.016642276324134286 1105 110-111 0.08129653595772623 1105 112-113 0.05704579308729052 1105 114-115 -0.025319218696417067 1105 116-117 -0.04031486668508322 1105 118-119 0.03890962829679623 1105 120-121 0.12311099735336484 1105 122-123 0.0066849327672322545 1105 124-125 -0.2987020679314014 1106 1 0.1503560717239978 1106 2 0.09762730207248183 1106 3 0.16691333043944212 1106 4 0.05526040542076771 1106 5 0.09249569762533838 1106 6 0.0292391846012805 1106 7 0.11215935905941166 1106 8 0.10428350860782842 1106 9 0.1849490736127848 1106 10-11 0.10013355040435101 1106 12-13 0.11911793143326577 1106 14-15 0.12494917117120252 1106 16-17 0.2220090047998582 1106 18-19 0.1915819137247965 1106 20-21 0.06086955183840104 1106 22-23 -0.03251196915794452 1106 24-25 0.033249404157793094 1106 26-27 0.12743891843091149 1106 28-29 0.030387471395428634 1106 30-31 0.009088984440687398 1106 32-33 0.07840922755960378 1106 34-35 0.08930819634963427 1106 36-37 0.18084871645595513 1106 38-39 0.09883163550138363 1106 40-41 -0.002530108864625902 1106 42-43 -0.029425896059507295 1106 44-45 0.2238515323560577 1106 46-47 0.21589441741030413 1106 48-49 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-0.4509776224408313 2216 36-37 -0.4004756427718448 2216 38-39 -0.3576506603292344 2216 40-41 -0.5826170169056937 2216 42-43 -0.39940628197287253 2216 44-45 -0.3940866566375618 2216 46-47 -0.5109261000843546 2216 48-49 -0.5122834313103439 2216 50-51 -0.38383729371354747 2216 52-53 -0.448022227079548 2216 54-55 -0.30670933309879445 2216 56-57 -0.42852706535440177 2216 58-59 -0.38688131997709974 2216 60-61 -0.4953179243304291 2216 62-63 -0.45398408673216295 2216 64-65 -0.40615698745565965 2216 66-67 -0.2927120382867088 2216 68-69 -0.44759062175751296 2216 70-71 -0.4553925866368971 2216 72-73 -0.43094748488283585 2216 74-75 -0.4935129306306507 2216 76-77 -0.3533073063433534 2216 78-79 -0.44257059378448105 2216 80-81 -0.3326251337170163 2216 82-83 -0.3047890533917581 2216 84-85 -0.2611981190775765 2216 86-87 -0.3510178501716297 2216 88-89 -0.25353409926197656 2216 90-91 -0.31014255044582484 2216 92-93 -0.24809700773416665 2216 94-95 -0.24871862938889677 2216 96-97 -0.32942885062135474 2216 98-99 -0.4548918799174402 2216 100-101 -0.44643764977691447 2216 102-103 -0.37573452930867646 2216 104-105 -0.39690225096052245 2216 106-107 -0.3858292067276565 2216 108-109 -0.21755619002554027 2216 110-111 -0.201285288003497 2216 112-113 -0.06302107856001982 2216 114-115 -0.1711990719994283 2216 116-117 -0.18949205623561127 2216 118-119 -0.16592769172275368 2216 120-121 -0.08604776544946446 2216 122-123 -0.1332309638278808 2216 124-125 0.03845005550629921 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 188.0 3 3.0 4 23.0 5 63.0 6 196.0 7 378.0 8 718.0 9 1242.0 10 1986.0 11 2762.0 12 3837.0 13 5527.0 14 8491.0 15 7485.0 16 3718.0 17 3277.0 18 3236.0 19 2914.0 20 2889.0 21 2889.0 22 3077.0 23 3511.0 24 4533.0 25 6034.0 26 8023.0 27 10661.0 28 13885.0 29 17708.0 30 22682.0 31 28406.0 32 36479.0 33 47228.0 34 65001.0 35 99202.0 36 200281.0 37 436289.0 38 218977.0 39 1206.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.15808058896584 18.52411777119989 13.182378410862352 24.135423228971923 2 16.01562346814326 19.980078509496042 38.91043564535041 25.093862377010286 3 19.375688722789324 24.278571456582522 29.404433708103106 26.941306112525048 4 12.769910706232523 16.338916317975226 36.582444774726376 34.30872820106588 5 13.258981751635792 38.23061336915937 34.30479021522303 14.205614663981805 6 32.790808774212984 36.49520976544206 16.663018981299533 14.05096247904542 7 29.011494072572262 32.67195030607723 20.08894082768303 18.227614793667477 8 27.085854565276215 35.435390449449216 18.75208332516343 18.726671660111137 9 26.755424488531414 15.502841165328762 19.333884965157 38.40784938098282 10-11 25.449743334339864 26.404876843620222 26.96989423570888 21.175485586331032 12-13 26.112172109128984 23.688809063493867 27.65918565025235 22.539833177124795 14-15 22.76524405786644 25.10848977062835 25.536370445606092 26.589895725899115 16-17 22.386304367433855 28.79561256622523 26.265544056689972 22.552539009650943 18-19 22.33352026070486 26.840914349355494 28.670671879718117 22.15489351022153 20-21 22.860069466639423 26.0718329130067 29.03700822078109 22.031089399572785 22-23 22.709754079395765 26.028603809396827 28.907690558076244 22.353951553131164 24-25 22.481558895847467 26.22895596487857 28.919063062497795 22.37042207677617 26-27 22.270797328473734 26.578596182043707 28.718279939278503 22.43232655020406 28-29 22.154492427849075 26.399783215856303 29.042643888122438 22.40308046817218 30-31 22.30610437779362 26.098762120559716 29.067186508580768 22.527946993065896 32-33 22.177207148207263 26.27585774173435 28.993964729550083 22.55297038050831 34-35 22.681180107952773 26.31619401381331 28.607759046652475 22.394866831581442 36-37 22.77928321849718 26.04762334265356 28.59537805734095 22.577715381508305 38-39 22.400539605727037 26.319896784718495 28.72141677875773 22.55814683079674 40-41 22.599428158432506 26.022438369777024 28.880430524308366 22.497702947482104 42-43 22.57622519127376 25.980525566566403 28.659769961686425 22.783479280473408 44-45 22.574831137784663 26.26054506495666 28.500224313021764 22.664399484236913 46-47 22.828773220497176 26.640170038548867 27.806322328147733 22.724734412806225 48-49 22.940392156862746 26.498509803921564 27.71662745098039 22.844470588235293 50-51 22.874027944988452 26.677816949737455 27.664754255865663 22.783400849408434 52-53 22.99057650754311 26.688483574574217 27.50526468523653 22.815675232646146 54-55 22.751910776820484 26.552091952580575 27.625930878702437 23.0700663918965 56-57 22.720233536430655 26.92343438787863 27.57770658848655 22.778625487204163 58-59 22.758613116988744 26.473506336847834 27.633660469818505 23.13422007634492 60-61 22.628879057979056 26.553966322445387 28.009491738650127 22.807662880925424 62-63 22.579833020262665 26.604130964192297 27.97738048086086 22.838655534684175 64-65 22.441245328449693 26.44013160732703 27.908008203889395 23.21061486033388 66-67 22.381363210340353 26.708914867000523 27.69738157889577 23.21234034376336 68-69 22.384016985051414 26.47549594961896 27.88827638512365 23.252210680205977 70-71 22.35557692368024 26.477014974070666 27.8106970644798 23.356711037769294 72-73 22.470849088198225 26.55699633647921 27.6026017193685 23.369552855954066 74-75 22.346422170893447 26.781659679765962 27.46373543633162 23.40818271300897 76-77 22.494155902979166 26.595945347643873 27.494373549269824 23.415525200107137 78-79 22.624813236026526 26.63746416680719 27.45565703663907 23.28206556052721 80-81 22.4838431372549 26.940549019607847 27.28878431372549 23.286823529411766 82-83 22.450304116454443 26.841463366810324 27.405892525911664 23.302339990823565 84-85 22.537362598578046 26.690758075458525 27.27832439872785 23.49355492723558 86-87 22.463441319837962 26.447308049772356 27.540284155748406 23.548966474641276 88-89 22.47920596389818 26.69420904231748 27.326990874545594 23.499594119238747 90-91 22.511362700538427 26.902757244089237 27.250638232791246 23.33524182258109 92-93 22.500107842714343 26.430876741659837 27.420990505919583 23.648024909706237 94-95 22.2835988878475 26.329386943580612 27.502598029027336 23.884416139544552 96-97 22.379245571585994 26.384171042466498 27.369657373892654 23.866926012054854 98-99 22.38487972778114 26.334006141943924 27.194552799266823 24.08656133100811 100-101 22.176431534163214 26.205201628700138 27.474059895474877 24.144306941661775 102-103 22.23425269028673 25.992046296343503 27.487061800222506 24.286639213147257 104-105 22.068177138991558 26.115471720310985 27.519014892302867 24.29733624839459 106-107 22.096148642554343 26.158877808322323 27.428833612417208 24.31613993670613 108-109 22.210143691379418 26.17880382544327 27.178263036675986 24.432789446501324 110-111 22.03685554850953 26.418045249599885 27.257450387289545 24.287648814601038 112-113 21.944188454162923 26.319857569186006 27.38173575789899 24.354218218752084 114-115 21.82371833835946 26.343386888678864 27.16330524194023 24.669589531021447 116-117 21.68007184285552 26.325583036929267 27.412363088772203 24.581982031443015 118-119 21.59030748899024 26.43577868322085 27.160599370198547 24.813314457590362 120-121 21.596588235294117 26.60709803921569 27.236039215686276 24.56027450980392 122-123 21.572681293919306 26.6523894808958 27.156195575745624 24.61873364943927 124-125 21.53925731242517 26.626572690527762 27.10598276319809 24.728187233848978 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 80.0 1 59.5 2 100.0 3 163.0 4 116.5 5 89.5 6 250.5 7 626.5 8 1159.0 9 1443.0 10 1145.5 11 779.0 12 684.5 13 777.0 14 910.5 15 991.0 16 1105.5 17 1216.5 18 1344.0 19 1473.0 20 1614.5 21 1886.0 22 2295.0 23 2852.0 24 3525.5 25 4268.0 26 5443.5 27 7112.5 28 8825.0 29 10725.5 30 13335.5 31 15729.5 32 18191.0 33 20599.0 34 22838.0 35 25277.5 36 28764.5 37 32215.0 38 33919.5 39 35703.0 40 37007.5 41 38895.5 42 41580.0 43 43556.0 44 45692.0 45 48441.0 46 49197.0 47 48439.5 48 48008.5 49 47124.5 50 46707.5 51 45726.0 52 45019.5 53 45206.5 54 45152.0 55 43046.0 56 38933.0 57 35452.0 58 32705.5 59 28857.5 60 23991.5 61 19707.0 62 16084.0 63 12549.0 64 9731.0 65 7558.5 66 5528.0 67 4577.0 68 3391.5 69 2070.0 70 1493.0 71 1100.5 72 827.5 73 622.5 74 491.0 75 435.5 76 280.0 77 118.0 78 55.5 79 24.5 80 12.5 81 5.5 82 4.5 83 2.5 84 1.0 85 0.5 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02854890765134254 2 0.0 3 0.0 4 0.0 5 0.05482331441837483 6 0.017568558554672334 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 3.52939792392971E-4 22-23 0.0 24-25 0.0 26-27 2.745087274167552E-4 28-29 3.137242599048631E-4 30-31 1.5686212995243154E-4 32-33 0.0 34-35 2.3529319492864733E-4 36-37 0.0 38-39 0.0 40-41 1.1764659746432367E-4 42-43 0.0 44-45 7.843106497621577E-5 46-47 0.0 48-49 3.921553248810789E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 3.9215532488107884E-5 58-59 1.5686212995243154E-4 60-61 1.1764659746432367E-4 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.1764659746432367E-4 70-71 7.843106497621577E-5 72-73 1.5686212995243154E-4 74-75 0.0 76-77 3.9215532488107884E-5 78-79 0.0 80-81 3.921553248810789E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 1.1764659746432367E-4 100-101 3.9215532488107884E-5 102-103 1.1764659746432367E-4 104-105 0.001372543637083776 106-107 0.0 108-109 0.0012156815071313447 110-111 0.00427449304120376 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 3.921553248810789E-4 122-123 8.235261822502656E-4 124-125 3.9215532488107884E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1275005.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.99366051968107 #Duplication Level Percentage of deduplicated Percentage of total 1 86.20554130986392 62.92458017287549 2 9.081721598548272 13.258162065973755 3 2.267129319781264 4.964582037669871 4 0.8901909192827765 2.5991317503931923 5 0.4236261087158363 1.546101018343863 6 0.24955285922974663 1.092946601299913 7 0.1621748594387554 0.8286415644289655 8 0.11748288218722813 0.6860404495398572 9 0.08006359564807866 0.525972142864878 >10 0.46777697453025924 6.415975133643196 >50 0.03580611915779534 1.8085844368984638 >100 0.017996153935498773 2.0440243460502865 >500 3.749198722356334E-4 0.19936694768702445 >1k 5.623798083534502E-4 1.1058913323311816 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3248 0.25474409904274886 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3141 0.24635197509029375 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1693 0.1327837930047333 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.921553248810789E-5 0.0 18-19 1.1764659746432367E-4 0.0 0.0 7.843106497621578E-5 0.0 20-21 2.3529319492864733E-4 0.0 0.0 1.1764659746432368E-4 0.0 22-23 2.3529319492864733E-4 0.0 0.0 1.5686212995243156E-4 0.0 24-25 2.3529319492864733E-4 0.0 0.0 2.3529319492864733E-4 0.0 26-27 2.3529319492864733E-4 0.0 0.0 6.274485198097263E-4 0.0 28-29 3.921553248810789E-4 0.0 0.0 0.0022745008843102574 0.0 30-31 3.921553248810789E-4 0.0 0.0 0.009764667589538865 0.0 32-33 3.921553248810789E-4 0.0 0.0 0.023529319492864735 0.0 34-35 3.921553248810789E-4 0.0 0.0 0.04258806828208517 0.0 36-37 3.921553248810789E-4 0.0 0.0 0.07525460684467904 0.0 38-39 3.921553248810789E-4 0.0 0.0 0.13399947451186467 0.0 40-41 3.921553248810789E-4 0.0 0.0 0.20203842337873185 0.0 42-43 3.921553248810789E-4 0.0 0.0 0.26497935302214504 0.0 44-45 3.921553248810789E-4 0.0 0.0 0.3357241736306916 0.0 46-47 4.7058638985729466E-4 0.0 0.0 0.4246257857812322 0.0 48-49 4.7058638985729466E-4 0.0 0.0 0.5258018596005506 0.0 50-51 4.7058638985729466E-4 0.0 0.0 0.6388210242312775 0.0 52-53 4.7058638985729466E-4 0.0 0.0 0.7459578589887883 0.0 54-55 4.7058638985729466E-4 0.0 0.0 0.8576044799824314 0.0 56-57 4.7058638985729466E-4 0.0 0.0 0.9978000086274172 0.0 58-59 4.7058638985729466E-4 0.0 0.0 1.1578385967113856 0.0 60-61 4.7058638985729466E-4 0.0 0.0 1.3315241901012151 0.0 62-63 4.7058638985729466E-4 0.0 0.0 1.50117058364477 0.0 64-65 4.7058638985729466E-4 0.0 0.0 1.6717973655005274 0.0 66-67 4.7058638985729466E-4 0.0 0.0 1.8735612801518426 0.0 68-69 4.7058638985729466E-4 0.0 0.0 2.104148611181917 0.0 70-71 4.7058638985729466E-4 0.0 0.0 2.3559515452880575 0.0 72-73 4.7058638985729466E-4 0.0 0.0 2.6099113336810444 0.0 74-75 4.7058638985729466E-4 0.0 0.0 2.8604985862800536 0.0 76-77 4.7058638985729466E-4 0.0 0.0 3.1290857682911044 0.0 78-79 4.7058638985729466E-4 0.0 0.0 3.435476723620692 0.0 80-81 4.7058638985729466E-4 0.0 0.0 3.770691095329038 0.0 82-83 5.490174548335104E-4 0.0 0.0 4.125121077956557 0.0 84-85 5.490174548335104E-4 0.0 0.0 4.4708452123717155 0.0 86-87 5.490174548335104E-4 0.0 0.0 4.816647777851852 0.0 88-89 5.490174548335104E-4 0.0 0.0 5.212999164709158 0.0 90-91 5.490174548335104E-4 0.0 0.0 5.635938682593402 0.0 92-93 5.490174548335104E-4 0.0 0.0 6.092093756495073 0.0 94-95 5.490174548335104E-4 0.0 0.0 6.5439743373555395 0.0 96-97 5.490174548335104E-4 0.0 0.0 6.986364759353885 0.0 98-99 5.882329873216183E-4 0.0 0.0 7.465853075085979 0.0 100-101 6.274485198097263E-4 0.0 0.0 8.008164673864023 7.843106497621578E-5 102-103 6.274485198097263E-4 0.0 0.0 8.56741738267693 7.843106497621578E-5 104-105 6.274485198097263E-4 0.0 0.0 9.120866192681596 7.843106497621578E-5 106-107 7.05879584785942E-4 0.0 0.0 9.655177822832067 7.843106497621578E-5 108-109 7.05879584785942E-4 0.0 0.0 10.21513641122976 7.843106497621578E-5 110-111 7.05879584785942E-4 0.0 0.0 10.829604589785923 7.843106497621578E-5 112-113 7.05879584785942E-4 0.0 0.0 11.47360206430563 7.843106497621578E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2525 0.0 69.33926 1 GTATCAA 4185 0.0 53.50392 1 ATCAACG 5040 0.0 43.35033 3 TCAACGC 5130 0.0 42.5898 4 CAACGCA 5200 0.0 41.90199 5 TATCAAC 5330 0.0 41.215073 2 AACGCAG 5375 0.0 40.629368 6 ACGCAGA 6285 0.0 34.837254 7 GTACATG 5950 0.0 34.22965 1 CGCAGAG 6370 0.0 34.185585 8 GTGGTAT 1095 0.0 33.17485 1 ACATGGG 6070 0.0 32.65972 3 TACATGG 6235 0.0 32.654766 2 TGGTATC 1170 0.0 31.038511 2 GCAGAGT 7260 0.0 29.830885 9 GAGTACT 4240 0.0 28.766657 12-13 CATGGGA 4045 0.0 28.110706 4 TATAACG 150 5.272268E-6 27.782013 2 GTACTTT 4430 0.0 27.197105 14-15 CAGAGTA 7115 0.0 27.093899 10-11 >>END_MODULE