FastQCFastQC Report
Thu 26 May 2016
SRR1364391_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364391_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1702436
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14550.055.2417341
GTATCAA23250.046.8624151
TCAACGC25700.041.2257464
ATCAACG25950.040.8285873
CAACGCA26550.039.9047285
AACGCAG28250.037.4967736
TATCAAC28850.037.3434452
GTACATG65000.036.8221551
TACATGG69550.033.8050542
ACATGGG69250.032.834113
ACGCAGA33700.031.4244447
CGCAGAG34500.030.5233158
CATGGGA35250.026.0041874
CATGGGG43250.025.735774
GAGTACA52000.023.9298211
GCAGAGT44500.022.9956659
TAAGGTA5500.022.7267994
AGTACAT53250.022.0205552
GCGTATA1653.2021932E-421.6502951
CAGAGTA42350.021.2129510-11
Produced by FastQC (version 0.11.5)