##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364391_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1702436 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.048656160936446 31.0 31.0 34.0 28.0 34.0 2 31.14668803996156 33.0 31.0 34.0 28.0 34.0 3 31.238344936314785 33.0 31.0 34.0 28.0 34.0 4 34.665048201518296 37.0 35.0 37.0 32.0 37.0 5 34.70493927525029 37.0 35.0 37.0 32.0 37.0 6 34.798676719712226 37.0 35.0 37.0 32.0 37.0 7 34.72868936042236 37.0 35.0 37.0 32.0 37.0 8 34.662279228117825 37.0 35.0 37.0 32.0 37.0 9 36.212707555526315 39.0 37.0 39.0 32.0 39.0 10-11 36.11976749786776 39.0 37.0 39.0 31.5 39.0 12-13 36.11759384787446 39.0 37.0 39.0 31.5 39.0 14-15 37.43293815450331 40.0 38.0 41.0 31.5 41.0 16-17 37.43546893980155 40.0 38.0 41.0 31.5 41.0 18-19 37.437058426865974 40.0 38.0 41.0 31.0 41.0 20-21 37.464838619484084 40.0 38.0 41.0 31.0 41.0 22-23 37.428852832059476 40.0 38.0 41.0 31.0 41.0 24-25 37.43326973818693 40.0 38.0 41.0 31.0 41.0 26-27 37.38623331508497 40.0 38.0 41.0 31.0 41.0 28-29 37.38942344969209 40.0 38.0 41.0 31.0 41.0 30-31 37.296706307902326 40.0 38.0 41.0 30.5 41.0 32-33 37.25650450295929 40.0 37.5 41.0 30.0 41.0 34-35 37.24246256540628 40.0 37.0 41.0 30.0 41.0 36-37 37.177073323167505 40.0 37.0 41.0 30.0 41.0 38-39 37.079170083339406 40.0 37.0 41.0 30.0 41.0 40-41 37.016617952157965 40.0 37.0 41.0 30.0 41.0 42-43 36.887957315282335 40.0 37.0 41.0 30.0 41.0 44-45 36.75635971043845 40.0 37.0 41.0 30.0 41.0 46-47 36.64027840106765 40.0 36.0 41.0 30.0 41.0 48-49 36.48887182836829 40.0 36.0 41.0 30.0 41.0 50-51 36.36001558942598 40.0 36.0 41.0 29.5 41.0 52-53 36.19968092779993 39.5 35.0 41.0 29.0 41.0 54-55 36.00913896322681 39.0 35.0 41.0 29.0 41.0 56-57 35.80585701900101 39.0 35.0 41.0 28.5 41.0 58-59 35.57415785380478 39.0 35.0 41.0 28.0 41.0 60-61 35.31835146813155 38.0 34.5 40.0 28.0 41.0 62-63 35.04233580586877 38.0 34.0 40.0 28.0 41.0 64-65 34.697539878151076 37.0 34.0 40.0 27.0 41.0 66-67 34.38202111562491 37.0 34.0 40.0 27.0 41.0 68-69 34.0517411520903 36.0 34.0 39.0 26.0 41.0 70-71 33.700678322121945 36.0 33.0 39.0 26.0 41.0 72-73 33.340383720738984 35.0 33.0 38.5 26.0 40.0 74-75 32.990758536591095 35.0 33.0 37.0 26.0 39.5 76-77 32.76701532392407 35.0 33.0 37.0 26.0 39.0 78-79 32.617887544671284 35.0 33.0 36.5 26.0 39.0 80-81 32.36319661943239 35.0 33.0 36.0 26.0 38.5 82-83 32.08205536067142 35.0 33.0 36.0 25.5 37.0 84-85 31.839608654892167 35.0 33.0 35.0 25.0 37.0 86-87 31.646625482543836 35.0 33.0 35.0 25.0 36.5 88-89 31.39576495092914 35.0 32.0 35.0 24.0 36.0 90-91 31.281673437356822 35.0 32.0 35.0 24.0 36.0 92-93 31.142572760444445 35.0 32.0 35.0 23.5 36.0 94-95 31.05044859248747 35.0 32.0 35.0 23.0 35.0 96-97 30.953268434173147 35.0 32.0 35.0 23.0 35.0 98-99 30.82841263930039 35.0 32.0 35.0 20.0 35.0 100-101 30.741288659309365 35.0 32.0 35.0 20.0 35.0 102-103 29.713055586230553 33.5 30.0 34.5 18.5 35.0 104-105 30.433079128965787 34.5 31.5 35.0 19.0 35.0 106-107 30.77704830020042 35.0 32.0 35.0 20.0 35.0 108-109 30.817452462236467 35.0 33.0 35.0 20.0 35.0 110-111 30.754176368450857 35.0 32.5 35.0 19.0 35.0 112-113 30.669315615976167 35.0 32.0 35.0 18.5 35.0 114-115 30.579936044585523 35.0 32.0 35.0 17.5 35.0 116-117 30.454672011165176 35.0 32.0 35.0 12.5 35.0 118-119 30.290474649267285 35.0 32.0 35.0 4.5 35.0 120-121 30.12116167656229 35.0 32.0 35.0 2.0 35.0 122-123 29.94540323395417 35.0 32.0 35.0 2.0 35.0 124-125 29.13596546360627 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12546403549776386 1101 2 0.09979633978122138 1101 3 0.08974231991687276 1101 4 0.1402040268725031 1101 5 0.01591593390031676 1101 6 0.15204913679464482 1101 7 0.1842623574201454 1101 8 -0.11711555403632445 1101 9 0.16262416979625982 1101 10-11 0.197130688629791 1101 12-13 0.32158014945724034 1101 14-15 0.3765157982188114 1101 16-17 0.3252864383713785 1101 18-19 0.15970651822194526 1101 20-21 0.286968070363919 1101 22-23 0.4284136551803783 1101 24-25 0.39512279824918295 1101 26-27 0.4350415757999926 1101 28-29 0.42534945545764913 1101 30-31 0.48011366485929585 1101 32-33 0.34520109134051324 1101 34-35 0.2972507262502262 1101 36-37 0.21113598188062355 1101 38-39 0.25038419733056116 1101 40-41 0.1835282231236448 1101 42-43 0.3124371261840011 1101 44-45 0.2794127079239459 1101 46-47 0.2435972855975379 1101 48-49 0.24460018265813233 1101 50-51 0.25223266298444713 1101 52-53 0.14106267502104686 1101 54-55 0.2317238026520414 1101 56-57 0.12232251015975493 1101 58-59 0.074599249752346 1101 60-61 0.07396242767183026 1101 62-63 0.049767250025460896 1101 64-65 0.062421072572938385 1101 66-67 -0.002314341572528633 1101 68-69 0.04853169701471671 1101 70-71 0.14010946955662718 1101 72-73 0.13483428379574747 1101 74-75 0.07654979028745856 1101 76-77 0.08282393743746042 1101 78-79 0.21808437136410674 1101 80-81 0.19728888893315855 1101 82-83 0.07161046175517072 1101 84-85 0.0644651994498382 1101 86-87 0.07927091204274106 1101 88-89 0.10601304118163668 1101 90-91 0.05670943338396128 1101 92-93 -0.029011872981261178 1101 94-95 0.00749402092464635 1101 96-97 0.008358773768957661 1101 98-99 -0.022742808459252473 1101 100-101 -0.04219168853621014 1101 102-103 -0.20643694697547943 1101 104-105 -0.09344999309866253 1101 106-107 -0.029777574867431156 1101 108-109 -0.021854910661488702 1101 110-111 -0.0650761390395651 1101 112-113 -0.05233724170765797 1101 114-115 -0.051050664746220775 1101 116-117 -0.1097671012065824 1101 118-119 -0.15893732654002335 1101 120-121 -0.2258849237512237 1101 122-123 -0.2236583161033998 1101 124-125 -0.29673513888694103 1102 1 0.12360182901256067 1102 2 0.12298261706738245 1102 3 0.15211354252818055 1102 4 0.1492315456036124 1102 5 0.21755080862372012 1102 6 0.21374363727996837 1102 7 0.23566052587610642 1102 8 0.20837965096913535 1102 9 0.2740007833993161 1102 10-11 0.30266141910558986 1102 12-13 0.18971950864468567 1102 14-15 0.17861379956118384 1102 16-17 0.2797046135847978 1102 18-19 0.23731033598937046 1102 20-21 0.20748739844622577 1102 22-23 0.11890002927132315 1102 24-25 0.2107960928215391 1102 26-27 0.19537721239728256 1102 28-29 0.15038633502324217 1102 30-31 0.24560470992788908 1102 32-33 0.2663028268609722 1102 34-35 0.24196466060655553 1102 36-37 0.06875180747837106 1102 38-39 0.13044089681450544 1102 40-41 0.03412472487062246 1102 42-43 -0.05146905779978539 1102 44-45 -0.009387085185927901 1102 46-47 0.024830827318318427 1102 48-49 -0.002190758053977504 1102 50-51 -0.09068724162995778 1102 52-53 -0.021342253495092223 1102 54-55 -0.03687954573909025 1102 56-57 -0.02854241818712211 1102 58-59 -0.07882731108656316 1102 60-61 -0.11653786170093383 1102 62-63 -0.019442072269391986 1102 64-65 -0.10902358106186938 1102 66-67 0.028274702885411784 1102 68-69 0.09428099343853802 1102 70-71 0.0523091680100265 1102 72-73 0.14480787893818814 1102 74-75 0.05980580022443149 1102 76-77 0.04444534607411299 1102 78-79 0.1640640267338469 1102 80-81 0.05709616797638972 1102 82-83 -0.0031019069285136425 1102 84-85 0.024949054142396676 1102 86-87 -0.005923081633373073 1102 88-89 0.06859696416486472 1102 90-91 -0.06807020063273228 1102 92-93 -0.05497013950496665 1102 94-95 -0.03969196731425484 1102 96-97 -0.06484993071993372 1102 98-99 -0.018324421668449276 1102 100-101 -0.04023866652760333 1102 102-103 -0.2752420750904676 1102 104-105 -0.14722132742283023 1102 106-107 -0.14604878712772518 1102 108-109 -0.13291651808974336 1102 110-111 -0.09576844953621233 1102 112-113 -0.19423543913344687 1102 114-115 -0.16736988879755188 1102 116-117 -0.21109492831986643 1102 118-119 -0.18445640723954426 1102 120-121 -0.2809990420822217 1102 122-123 -0.3092861247931751 1102 124-125 -0.3725419322786969 1103 1 0.11089762139758008 1103 2 0.1710208941616287 1103 3 0.31177053157282586 1103 4 0.22576016697937717 1103 5 0.2528597786757416 1103 6 0.24183067585779128 1103 7 0.21415359056138783 1103 8 0.25001492386461166 1103 9 0.36858477910030274 1103 10-11 0.4142970458874018 1103 12-13 0.4236732967016934 1103 14-15 0.4590524284215647 1103 16-17 0.283440374921355 1103 18-19 0.2741530993022323 1103 20-21 0.3485016709783011 1103 22-23 0.3830222376031216 1103 24-25 0.3990014978039156 1103 26-27 0.4387461697686277 1103 28-29 0.3447390730199942 1103 30-31 0.472115894217076 1103 32-33 0.4080678217031135 1103 34-35 0.5293788256320084 1103 36-37 0.3297064213221219 1103 38-39 0.297533552207625 1103 40-41 0.37984852297831395 1103 42-43 0.2813669639004601 1103 44-45 0.21319790950344242 1103 46-47 0.2280119380817851 1103 48-49 0.2495182245321672 1103 50-51 0.16142715127023877 1103 52-53 0.06212027952975063 1103 54-55 0.11705196774546067 1103 56-57 0.04768992081914547 1103 58-59 0.10395607197013845 1103 60-61 0.06954679757590299 1103 62-63 0.13169841608439725 1103 64-65 0.1501730595434836 1103 66-67 0.10030616166568507 1103 68-69 -0.00433013521098502 1103 70-71 0.04860879530691875 1103 72-73 0.08094721911010794 1103 74-75 0.11538037522175415 1103 76-77 0.10410286516876965 1103 78-79 0.16168449190261924 1103 80-81 0.23416475442185458 1103 82-83 0.11679174321259467 1103 84-85 0.002115746610027003 1103 86-87 0.07980985827813214 1103 88-89 0.12475521776854137 1103 90-91 0.0809262354044904 1103 92-93 0.053591275129932114 1103 94-95 0.04372410838561791 1103 96-97 0.01998932927412156 1103 98-99 0.06961025247316499 1103 100-101 6.52899425663378E-4 1103 102-103 -0.199962033297588 1103 104-105 0.002554749829776881 1103 106-107 0.028282480725415837 1103 108-109 0.05341539393005945 1103 110-111 0.1357250490105919 1103 112-113 0.16465752958159996 1103 114-115 0.09908781344497797 1103 116-117 0.03323279599301543 1103 118-119 0.0464445265682798 1103 120-121 -0.05733711047707146 1103 122-123 -0.09057462649611736 1103 124-125 -0.1948722847042781 1104 1 0.25739961516124055 1104 2 0.32774537120079117 1104 3 0.26177959763315783 1104 4 0.3178619506690268 1104 5 0.3430504553178437 1104 6 0.2506455339762539 1104 7 0.2788941888721084 1104 8 0.33569981469785404 1104 9 0.38358764668441125 1104 10-11 0.3930842348207122 1104 12-13 0.32424970727446834 1104 14-15 0.43364591027820865 1104 16-17 0.4577344612948906 1104 18-19 0.4031127280893898 1104 20-21 0.4497331621312739 1104 22-23 0.4323110521164537 1104 24-25 0.3966557893751883 1104 26-27 0.3944168288881471 1104 28-29 0.38328298984743014 1104 30-31 0.5016899525397136 1104 32-33 0.3868282280473281 1104 34-35 0.3796743884342533 1104 36-37 0.36015637805438416 1104 38-39 0.3003730453825284 1104 40-41 0.3450857228247628 1104 42-43 0.3102486769025461 1104 44-45 0.17651638311751583 1104 46-47 0.22459815847047793 1104 48-49 0.22443363618825174 1104 50-51 0.12105393743035364 1104 52-53 0.21164470386570855 1104 54-55 0.16864716337995134 1104 56-57 0.132959604559872 1104 58-59 0.18625612070213293 1104 60-61 0.12256901265610054 1104 62-63 0.19657300430208835 1104 64-65 0.204724268007773 1104 66-67 0.07978584984246595 1104 68-69 0.15381974508314755 1104 70-71 0.02472103467307818 1104 72-73 -0.05880756319824343 1104 74-75 -0.07564875038212193 1104 76-77 -0.0399055002344042 1104 78-79 0.08339567461867858 1104 80-81 0.12581856082806553 1104 82-83 0.06137895117078074 1104 84-85 0.03844902237618797 1104 86-87 -0.061085031142098956 1104 88-89 0.041430888926374365 1104 90-91 0.06315683411234119 1104 92-93 0.06607149988203176 1104 94-95 0.052014397172733595 1104 96-97 0.08994121912441244 1104 98-99 0.0030766829604971235 1104 100-101 -0.052592888704356966 1104 102-103 -0.16611992312509472 1104 104-105 -0.04613214908195573 1104 106-107 -0.09489788092573548 1104 108-109 -0.06991450384356668 1104 110-111 -0.04589430636634617 1104 112-113 -0.08559559666998595 1104 114-115 -0.07225864889824152 1104 116-117 -0.06409328572343753 1104 118-119 -0.1317630501953957 1104 120-121 -0.25290734720129393 1104 122-123 -0.25681431096643337 1104 124-125 -0.46596434323562264 1105 1 -0.03736700896255485 1105 2 -0.07588568384831618 1105 3 -0.24819737857558977 1105 4 -0.05204880576410176 1105 5 -0.08829325027665647 1105 6 -0.016153423532522027 1105 7 0.083050287644717 1105 8 0.035910640039581665 1105 9 0.04007768508151344 1105 10-11 -0.01656577220278166 1105 12-13 0.09290913662928801 1105 14-15 0.0855614848872861 1105 16-17 0.04882508604809743 1105 18-19 -0.050261230971791804 1105 20-21 0.006811395394407782 1105 22-23 -0.007635074985245183 1105 24-25 -0.06291858699597697 1105 26-27 0.019851426309415388 1105 28-29 0.09115409399467467 1105 30-31 -0.031986637648259375 1105 32-33 0.07861794649873843 1105 34-35 -0.0799072224353381 1105 36-37 -0.07227521524140457 1105 38-39 -0.038936687007343096 1105 40-41 -0.01805263876632779 1105 42-43 -0.05786867751371716 1105 44-45 -0.09132238282174399 1105 46-47 -0.10527854156129735 1105 48-49 -0.22652656261575999 1105 50-51 -0.15080570244837332 1105 52-53 -0.10437717808054003 1105 54-55 -0.044571591574921854 1105 56-57 -0.14331669660989377 1105 58-59 -0.10413498834044077 1105 60-61 -0.1809352276642926 1105 62-63 -0.14168079275456336 1105 64-65 -0.16999773131821172 1105 66-67 -0.2672206352825057 1105 68-69 -0.3800788537753661 1105 70-71 -0.34530019857981387 1105 72-73 -0.29662979227983755 1105 74-75 -0.3944200431689069 1105 76-77 -0.33149678242264713 1105 78-79 -0.1458518651997096 1105 80-81 -0.181006917146199 1105 82-83 -0.1826646451225038 1105 84-85 -0.15533832195398745 1105 86-87 -0.07945131259182148 1105 88-89 -0.11175177793884217 1105 90-91 -0.040943830316052754 1105 92-93 -0.027312159435791017 1105 94-95 -0.1524849785503406 1105 96-97 -0.12371130429878363 1105 98-99 -0.2329013518965226 1105 100-101 -0.255326293217113 1105 102-103 -0.366413643761323 1105 104-105 -0.30404292239558117 1105 106-107 -0.247283999370147 1105 108-109 -0.18237553551921692 1105 110-111 -0.2878611494542973 1105 112-113 -0.1750266576272672 1105 114-115 -0.2168321119139769 1105 116-117 -0.3207598760259529 1105 118-119 -0.2921072676306551 1105 120-121 -0.42685573749524863 1105 122-123 -0.5371720750000009 1105 124-125 -0.6495493868747211 1106 1 0.30719170661465967 1106 2 0.40011227201650357 1106 3 0.31147500831988495 1106 4 0.3186985535882414 1106 5 0.31444641783324556 1106 6 0.31601639900272005 1106 7 0.3132026909128811 1106 8 0.3819227994576124 1106 9 0.48596539490255 1106 10-11 0.399937494369361 1106 12-13 0.34117827006548396 1106 14-15 0.4384407375279338 1106 16-17 0.4152585297173417 1106 18-19 0.436847880258334 1106 20-21 0.28576078589163956 1106 22-23 0.34066831119576335 1106 24-25 0.39723946583045233 1106 26-27 0.417310565052027 1106 28-29 0.34307935560843106 1106 30-31 0.5174784006547597 1106 32-33 0.4115386945933892 1106 34-35 0.5017818974468184 1106 36-37 0.4597353228391938 1106 38-39 0.4801242914569315 1106 40-41 0.48409305978746175 1106 42-43 0.46909861877436043 1106 44-45 0.4250763485399247 1106 46-47 0.42293815269956525 1106 48-49 0.34828259628633873 1106 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-0.7188499627709746 2216 100-101 -0.5820947100031191 2216 102-103 -0.5203528367255963 2216 104-105 -0.7333146221911875 2216 106-107 -0.551326536589066 2216 108-109 -0.48730184569186363 2216 110-111 -0.4021884653093899 2216 112-113 -0.42810436942830776 2216 114-115 -0.4660883546279635 2216 116-117 -0.5504754268384069 2216 118-119 -0.49936576355723616 2216 120-121 -0.32433854743282353 2216 122-123 -0.3935214682339385 2216 124-125 -0.24098738153711352 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 28037.0 3 5411.0 4 3392.0 5 2816.0 6 3030.0 7 3509.0 8 3885.0 9 4433.0 10 4783.0 11 5285.0 12 5868.0 13 5777.0 14 5824.0 15 5620.0 16 5124.0 17 4755.0 18 4590.0 19 4400.0 20 4422.0 21 4668.0 22 5540.0 23 7188.0 24 10442.0 25 15169.0 26 18165.0 27 18680.0 28 21888.0 29 26695.0 30 31838.0 31 39410.0 32 50634.0 33 66914.0 34 94353.0 35 144924.0 36 286050.0 37 527754.0 38 220459.0 39 704.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.27329874376274 18.286987657411636 12.731441896161943 24.70827170266368 2 15.895085480378462 19.61296204697044 39.20313089289428 25.288821579756814 3 18.830339211144796 24.227160505430305 29.03152096081335 27.910979322611553 4 12.68525991900551 15.936318169482206 36.79762773315288 34.58079417835941 5 13.654221602322353 38.122999858407454 34.425848101161705 13.796930438108488 6 33.29704175666116 35.9604478611668 17.116071155776062 13.62643922639598 7 29.154012887763674 31.84986988880339 21.112259822954787 17.88385740047815 8 26.505035434539355 35.37215811470041 19.756877648779543 18.36592880198069 9 26.548839427737665 16.068327972387802 19.38064044698303 38.0021921528915 10-11 24.997819299362178 26.480901321019985 27.692284196543245 20.828995183074593 12-13 25.492990260318127 23.788384397007313 27.628542243175435 23.09008309949912 14-15 22.62744738690858 25.519463965583274 24.75520256950661 27.097886078001537 16-17 23.08525504381083 28.550609627515446 25.621360723153273 22.742774605520452 18-19 23.18440258089073 27.64257441765048 26.518948882351197 22.654074119107594 20-21 23.603368116844404 26.985347458199577 26.664042973740553 22.747241451215462 22-23 23.57621666835053 26.736423571869956 26.510835062228477 23.176524697551038 24-25 23.33039244940779 26.985860261413645 26.42381270132915 23.25993458784941 26-27 23.124547156118396 27.216860785621538 26.38264415891233 23.27594789934773 28-29 23.164648566222557 27.255369690077575 26.451458095682277 23.128523648017595 30-31 23.236378032863534 26.98398293267169 26.3695097724787 23.410129261986075 32-33 23.037493094443924 27.10440672131981 26.440357288551947 23.417742895684324 34-35 23.667626573309505 26.95382609193664 26.134669860155164 23.243877474598698 36-37 23.596993244374374 26.82877975227219 26.09579936479145 23.47842763856199 38-39 23.156874216562876 27.02189923368542 26.347834923409657 23.47339162634205 40-41 23.3896147645728 26.69240052360557 26.51756184481386 23.400422867007766 42-43 23.11364006158296 26.771435234216725 26.387011658117064 23.72791304608325 44-45 23.14597257287057 26.8433366502154 26.52311263732068 23.487578139593353 46-47 23.50884952763262 27.135594858072448 26.12947744569471 23.22607816860022 48-49 23.362405400261743 26.989032186819355 26.141481970541037 23.507080442377866 50-51 23.19452831119643 27.073822452062807 26.336173577156497 23.395475659584267 52-53 23.356548386689383 26.896782835917556 26.30529973440679 23.441369042986274 54-55 23.113055844612425 26.88616583121644 26.367713821069437 23.633064503101693 56-57 23.097086497550126 27.17797531830134 26.379325384133494 23.345612800015036 58-59 23.164364161391337 26.774252667230964 26.31854870211305 23.742834469264654 60-61 22.977417374729427 26.87508627449829 26.590608047978904 23.556888302793386 62-63 23.01927899235384 26.972526804442843 26.627841611632363 23.380352591570954 64-65 22.988661037961307 26.92042343638072 26.40541657402809 23.685498951629885 66-67 22.85844271151973 27.00746627459008 26.389745936371163 23.74434507751903 68-69 22.896827891015402 26.68595938953306 26.760179491050412 23.65703322840113 70-71 22.940335443881906 26.605717454348298 26.56862278628831 23.885324315481487 72-73 22.916650757669654 26.8122893036911 26.330613824908617 23.94044611373063 74-75 22.886445212445853 26.965131841927725 26.410297001553985 23.738125944072436 76-77 22.983234654626482 26.71899638776899 26.576492567450682 23.721276390153847 78-79 23.00615136637637 26.66821743129936 26.657879000414713 23.667752201909558 80-81 23.049290345318777 26.846196206853513 26.542480156836255 23.56203329099146 82-83 22.953316592507747 26.837393717784924 26.535765158523873 23.67352453118346 84-85 23.021242339238928 26.751537732488146 26.494081532495844 23.733138395777083 86-87 23.010111969153925 26.68251107194392 26.68638797783232 23.620988981069832 88-89 23.03916918112659 26.694121347889542 26.568293103443214 23.69841636754066 90-91 22.97742250688797 26.82951471297863 26.65062289069331 23.542439889440097 92-93 22.95034975242574 26.40376029748267 26.790562954996084 23.855326995095506 94-95 22.71315370562908 26.42421625812818 26.90626817276684 23.956361863475895 96-97 22.94231688165831 26.345128332698458 26.848555638541484 23.863999147101747 98-99 22.850480053335996 26.321402243283803 26.658717175306844 24.169400528073353 100-101 22.7090484395986 26.286853518726666 26.976263195720623 24.027834845954104 102-103 22.588382766671142 26.094587644989264 27.098340852573454 24.21868873576614 104-105 22.49171767248975 26.380006108950788 26.890749638749543 24.237526579809916 106-107 22.572392562174027 26.2943308708784 26.805639551465273 24.327637015482303 108-109 22.610354693999106 26.230250640714242 26.75921068939854 24.400183975888115 110-111 22.563924987319414 26.441232714532465 26.837436791645704 24.157405506502418 112-113 22.46110838781796 26.480307261872486 27.15142198489595 23.907162365413594 114-115 22.42712840508982 26.670398701111036 26.778482804641506 24.123990089157637 116-117 22.312476944368083 26.50275727735602 27.069837711847484 24.114928066428416 118-119 22.21725638220631 26.62959623019284 26.792176069293745 24.36097131830711 120-121 22.202166754248193 26.758060853936044 26.961853129157248 24.07791926265852 122-123 22.169253131910462 26.8044776781348 27.00660937154431 24.01965981841043 124-125 22.150450470295393 26.8441526973148 26.911499291436293 24.093897540953513 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 492.0 1 441.5 2 346.5 3 267.0 4 210.0 5 176.5 6 160.5 7 156.5 8 156.5 9 156.0 10 162.5 11 168.5 12 170.0 13 182.5 14 204.5 15 220.5 16 261.0 17 337.5 18 413.5 19 505.0 20 697.0 21 1025.0 22 1460.5 23 2134.5 24 3127.5 25 4480.5 26 6316.5 27 8574.0 28 10547.5 29 12919.0 30 16244.0 31 19803.0 32 24117.0 33 27340.0 34 29672.0 35 33153.0 36 38099.5 37 42671.0 38 45473.5 39 47894.5 40 49339.5 41 50774.5 42 51896.0 43 53698.5 44 57294.0 45 60678.5 46 62139.5 47 62322.0 48 62068.0 49 61472.0 50 61139.5 51 60455.0 52 61359.0 53 63637.0 54 66253.0 55 63799.0 56 57591.0 57 53476.0 58 50330.5 59 44617.5 60 36455.0 61 30359.5 62 25089.5 63 19242.5 64 14512.5 65 11223.0 66 8359.0 67 6611.5 68 4913.5 69 3156.0 70 2150.5 71 1496.5 72 1154.0 73 833.5 74 590.0 75 455.5 76 304.0 77 178.5 78 108.5 79 60.0 80 31.5 81 21.0 82 17.5 83 18.0 84 16.0 85 10.0 86 7.5 87 4.0 88 3.0 89 4.5 90 3.0 91 1.5 92 1.5 93 2.0 94 1.0 95 1.0 96 1.0 97 1.0 98 1.5 99 0.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.045053088632994134 2 0.010220648529518877 3 0.002643271171427296 4 0.005756457217775 5 0.021674823605703827 6 0.028194892495224493 7 0.002702010530792347 8 5.8739359365051024E-5 9 0.0 10-11 8.810903904757653E-5 12-13 0.0011160478279359694 14-15 4.699148749204082E-4 16-17 1.7621807809515305E-4 18-19 2.9369679682525512E-5 20-21 5.8739359365051024E-5 22-23 0.0 24-25 0.0 26-27 2.9369679682525512E-5 28-29 5.873935936505102E-4 30-31 1.1747871873010205E-4 32-33 1.4684839841262756E-4 34-35 7.929813514281887E-4 36-37 1.7621807809515305E-4 38-39 1.1747871873010205E-4 40-41 5.580239139679847E-4 42-43 9.985691092058674E-4 44-45 0.0019971382184117347 46-47 2.9369679682525512E-5 48-49 0.0 50-51 0.0 52-53 0.001204156866983546 54-55 0.003553731241585587 56-57 2.9369679682525512E-5 58-59 8.810903904757654E-4 60-61 0.0012335265466660715 62-63 7.636116717456632E-4 64-65 0.0018502898199991072 66-67 0.0027901195698399233 68-69 0.003935537077458418 70-71 0.002055877577776786 72-73 0.002643271171427296 74-75 0.002202725976189413 76-77 0.001497853663808801 78-79 0.002936967968252551 80-81 0.0013803749450786989 82-83 6.461329530155612E-4 84-85 6.167632733330358E-4 86-87 0.0027901195698399233 88-89 0.006784396006663394 90-91 6.167632733330358E-4 92-93 0.001027938788888393 94-95 0.0015272233434913264 96-97 3.230664765077806E-4 98-99 0.0012335265466660715 100-101 0.002202725976189413 102-103 0.001497853663808801 104-105 9.398297498408164E-4 106-107 0.008076661912694516 108-109 0.00229083501523699 110-111 0.0017915504606340561 112-113 0.0026139014917447704 114-115 0.0034068828431729594 116-117 0.0018796594996816328 118-119 0.04760825076537385 120-121 0.012482113865073342 122-123 0.004728518428886608 124-125 0.0028488589292049744 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1702436.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.12068949126746 #Duplication Level Percentage of deduplicated Percentage of total 1 85.63062805816253 61.75739937124954 2 9.071153597738798 13.08435703900227 3 2.4606434095161105 5.323898978593352 4 1.0166129483364197 2.932753071190914 5 0.5114477549880053 1.8442982364247882 6 0.29283302886228035 1.267159196841833 7 0.18907200696344836 0.9545202453991132 8 0.1404380807776116 0.8102792973209332 9 0.09778122812022787 0.6346844632200368 >10 0.5294035433876193 7.031715601490469 >50 0.04296730236866031 2.1140577126494406 >100 0.01652334058266561 1.9747241405076534 >500 4.957001956468122E-4 0.270152646109689 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.1747871873010205E-4 2 0.0 0.0 0.0 0.0 1.1747871873010205E-4 3 0.0 0.0 0.0 0.0 1.1747871873010205E-4 4 0.0 0.0 0.0 0.0 1.1747871873010205E-4 5 0.0 0.0 0.0 0.0 1.1747871873010205E-4 6 0.0 0.0 0.0 0.0 1.1747871873010205E-4 7 0.0 0.0 0.0 0.0 1.1747871873010205E-4 8 0.0 0.0 0.0 0.0 1.1747871873010205E-4 9 0.0 0.0 0.0 0.0 1.1747871873010205E-4 10-11 0.0 0.0 0.0 0.0 1.1747871873010205E-4 12-13 0.0 0.0 0.0 0.0 1.1747871873010205E-4 14-15 0.0 0.0 0.0 0.0 2.3495743746020407E-4 16-17 0.0 0.0 0.0 0.0 3.524361561903061E-4 18-19 0.0 0.0 0.0 0.0 3.524361561903061E-4 20-21 0.0 0.0 0.0 0.0 6.755026326980868E-4 22-23 0.0 0.0 0.0 1.4684839841262756E-4 7.048723123806122E-4 24-25 0.0 0.0 0.0 3.230664765077806E-4 7.048723123806122E-4 26-27 0.0 0.0 0.0 7.929813514281889E-4 7.929813514281887E-4 28-29 0.0 0.0 0.0 0.003201295085395281 8.223510311107143E-4 30-31 0.0 0.0 0.0 0.010573084685709184 8.223510311107143E-4 32-33 0.0 0.0 0.0 0.02663829947205064 8.223510311107143E-4 34-35 0.0 0.0 0.0 0.051132612327276915 8.223510311107143E-4 36-37 0.0 0.0 0.0 0.09545145896820792 8.223510311107143E-4 38-39 0.0 0.0 0.0 0.15915429419960575 8.223510311107143E-4 40-41 0.0 0.0 0.0 0.22547103092274834 8.223510311107143E-4 42-43 0.0 0.0 0.0 0.293021294192557 8.517207107932398E-4 44-45 0.0 0.0 0.0 0.3803667215683879 8.810903904757653E-4 46-47 0.0 0.0 0.0 0.4883590337610342 9.104600701582908E-4 48-49 0.0 0.0 0.0 0.6027245664447884 0.0010573084685709185 50-51 0.0 0.0 0.0 0.7259010030333005 0.0011160478279359694 52-53 0.0 0.0 0.0 0.8431447643259424 0.0011160478279359694 54-55 0.0 0.0 0.0 0.9749558867411168 0.0011160478279359694 56-57 0.0 0.0 0.0 1.1287942689181856 0.0011160478279359694 58-59 0.0 0.0 0.0 1.2976992967723897 0.0011160478279359694 60-61 0.0 0.0 0.0 1.4848428957094422 0.0011160478279359694 62-63 0.0 0.0 0.0 1.664908401843006 0.0012335265466660715 64-65 0.0 0.0 0.0 1.8494087296086312 0.0012628962263485969 66-67 0.0 0.0 0.0 2.0716784654459843 0.001321635585713648 68-69 0.0 0.0 0.0 2.3189711683728493 0.0013510052653961735 70-71 0.0 0.0 0.0 2.5872631922727196 0.001380374945078699 72-73 0.0 0.0 0.0 2.8590502080548106 0.0014684839841262755 74-75 0.0 0.0 0.0 3.135301415148646 0.0015272233434913266 76-77 0.0 0.0 0.0 3.4386020972300866 0.0015272233434913266 78-79 0.0 0.0 0.0 3.7783799214772245 0.001615332382538903 80-81 0.0 0.0 0.0 4.150141326898632 0.0016740717419039541 82-83 0.0 0.0 0.0 4.535647742411462 0.0017034414215864797 84-85 0.0 0.0 0.0 4.911843969464931 0.0017034414215864797 86-87 0.0 0.0 0.0 5.3005516800631565 0.0017034414215864797 88-89 0.0 0.0 0.0 5.732051013958822 0.0017034414215864797 90-91 0.0 0.0 0.0 6.203405003183674 0.0017034414215864797 92-93 0.0 0.0 0.0 6.685155859016139 0.0017034414215864797 94-95 0.0 0.0 0.0 7.165849406380034 0.0017621807809515308 96-97 0.0 0.0 0.0 7.656234948039163 0.0018796594996816325 98-99 0.0 0.0 0.0 8.168060355866535 0.001967768538729209 100-101 0.0 0.0 0.0 8.736246179004674 0.0019971382184117347 102-103 0.0 0.0 0.0 9.336591801395176 0.0020265078980942603 104-105 0.0 0.0 0.0 9.931151596888224 0.002055877577776786 106-107 0.0 0.0 0.0 10.515520113531434 0.002055877577776786 108-109 0.0 0.0 0.0 11.125469621178123 0.002055877577776786 110-111 0.0 0.0 0.0 11.77700659525527 0.002114616937141837 112-113 0.0 0.0 0.0 12.461995634490812 0.002114616937141837 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1455 0.0 55.241734 1 GTATCAA 2325 0.0 46.862415 1 TCAACGC 2570 0.0 41.225746 4 ATCAACG 2595 0.0 40.828587 3 CAACGCA 2655 0.0 39.904728 5 AACGCAG 2825 0.0 37.496773 6 TATCAAC 2885 0.0 37.343445 2 GTACATG 6500 0.0 36.822155 1 TACATGG 6955 0.0 33.805054 2 ACATGGG 6925 0.0 32.83411 3 ACGCAGA 3370 0.0 31.424444 7 CGCAGAG 3450 0.0 30.523315 8 CATGGGA 3525 0.0 26.004187 4 CATGGGG 4325 0.0 25.73577 4 GAGTACA 5200 0.0 23.929821 1 GCAGAGT 4450 0.0 22.995665 9 TAAGGTA 550 0.0 22.726799 4 AGTACAT 5325 0.0 22.020555 2 GCGTATA 165 3.2021932E-4 21.650295 1 CAGAGTA 4235 0.0 21.21295 10-11 >>END_MODULE