FastQCFastQC Report
Thu 26 May 2016
SRR1364391_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364391_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1702436
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31450.18473528520308546No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30150.17709916848562882No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25550.070.639631
GTATCAA47850.054.3996621
ATCAACG58800.043.543353
TCAACGC59350.043.2401584
CAACGCA60800.042.013075
TATCAAC61650.041.626982
AACGCAG63250.040.4560436
ACGCAGA74450.034.2840047
GTGGTAT13050.034.2331961
CGCAGAG75650.033.897478
GTACATG59750.031.1037981
GCAGAGT86600.029.0617289
ACATGGG61600.028.9982173
TACATGG64500.028.8021972
TGGTATC14600.026.9166982
GAGTACT51800.026.41782612-13
CAGAGTA83400.025.39702810-11
AGAGTAC81350.024.72054910-11
GTACTTT56350.024.12632814-15
CATGGGA31700.023.6669334
Produced by FastQC (version 0.11.5)