FastQCFastQC Report
Thu 26 May 2016
SRR1364390_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364390_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1946863
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24150.044.3771361
ATCAACG24200.042.299813
CAACGCA24850.041.193385
TCAACGC25100.040.7830854
GGTATCA17800.038.8009071
AACGCAG26550.038.554776
GTACATG62750.036.2454571
TATCAAC30550.034.4815982
ACATGGG66600.032.6169973
TACATGG69250.032.2282562
CATGGGG46350.031.7155724
ACGCAGA32700.031.4751287
CGCAGAG33600.030.2779188
GCAGAGT45100.022.6892999
ATGGGGG27150.022.5783775
ATGGGGA27300.021.5823085
GTATAGG6400.021.3969441
GAGTACA54450.021.3226241
AGGTAAG9800.021.2553042
TAAGGTG10900.020.7482975
Produced by FastQC (version 0.11.5)