FastQCFastQC Report
Thu 26 May 2016
SRR1364390_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364390_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1946863
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28430.14602979254318357No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26940.1383764548404279No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30300.064.0888441
GTATCAA45950.050.1687051
ATCAACG55900.040.4846043
CAACGCA56700.039.91345
TCAACGC57750.039.1876954
AACGCAG59200.038.4004336
TATCAAC61750.037.4207842
GTACATG64300.035.0177541
ACATGGG64850.033.2442673
TACATGG69600.032.173382
ACGCAGA71250.031.731667
CGCAGAG73500.030.6793378
TAAGGTG11100.027.8996355
CATGGGG47600.027.0249544
GCAGAGT83950.026.5769219
GAGTACT52700.025.00674612-13
CATGGGA26700.024.7588164
CAGAGTA83000.024.30052810-11
GTACTTT54450.023.76596614-15
GTGGTAT16100.023.308921
Produced by FastQC (version 0.11.5)