FastQCFastQC Report
Thu 26 May 2016
SRR1364389_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364389_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1397680
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12500.050.9751551
GTATCAA19850.048.6003461
ATCAACG22950.039.6803863
TCAACGC23250.039.168384
CAACGCA23700.038.4233065
AACGCAG24250.037.2997326
GTACATG57000.036.9839441
TATCAAC26800.034.6463052
ACATGGG58000.034.480893
TACATGG61550.033.749282
ACGCAGA28600.031.6174727
CGCAGAG29150.030.8168338
CATGGGA37950.029.9563394
CATGGGG25550.027.2560024
CGTACCC900.00951360226.453593
ATGGGAT18000.025.460675
ATGGGAG18600.023.6793845
GTATAGA3800.023.5067441
GCAGAGT38200.022.737319
GAGTACA44550.022.055711
Produced by FastQC (version 0.11.5)