##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364389_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1397680 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.006610239825996 31.0 31.0 34.0 28.0 34.0 2 31.05759329746437 33.0 31.0 34.0 27.0 34.0 3 31.18265053517257 33.0 31.0 34.0 27.0 34.0 4 34.569017228550166 37.0 35.0 37.0 32.0 37.0 5 34.66919824280236 37.0 35.0 37.0 32.0 37.0 6 34.71327843283155 37.0 35.0 37.0 32.0 37.0 7 34.643200160265586 37.0 35.0 37.0 32.0 37.0 8 34.54664086199988 37.0 35.0 37.0 31.0 37.0 9 36.06890060671971 39.0 37.0 39.0 31.0 39.0 10-11 35.993848377310975 39.0 37.0 39.0 30.5 39.0 12-13 35.98613631160208 39.0 37.0 39.0 31.0 39.0 14-15 37.304807609753304 40.0 38.0 41.0 31.0 41.0 16-17 37.31007025928682 40.0 38.0 41.0 31.0 41.0 18-19 37.324198672085174 40.0 38.0 41.0 31.0 41.0 20-21 37.357684877797496 40.0 38.0 41.0 31.0 41.0 22-23 37.33982885925248 40.0 38.0 41.0 31.0 41.0 24-25 37.364576297865035 40.0 38.0 41.0 31.0 41.0 26-27 37.33437195924675 40.0 38.0 41.0 31.0 41.0 28-29 37.34162970064679 40.0 38.0 41.0 30.5 41.0 30-31 37.24626452406846 40.0 37.5 41.0 30.0 41.0 32-33 37.203303331234615 40.0 37.0 41.0 30.0 41.0 34-35 37.188929869498025 40.0 37.0 41.0 30.0 41.0 36-37 37.12761254364375 40.0 37.0 41.0 30.0 41.0 38-39 37.02761826741457 40.0 37.0 41.0 30.0 41.0 40-41 36.962710348577644 40.0 37.0 41.0 30.0 41.0 42-43 36.835162197355615 40.0 37.0 41.0 30.0 41.0 44-45 36.721719563848666 40.0 36.5 41.0 30.0 41.0 46-47 36.61842768015569 40.0 36.0 41.0 30.0 41.0 48-49 36.47205404670597 40.0 36.0 41.0 29.5 41.0 50-51 36.35112901379429 40.0 36.0 41.0 29.0 41.0 52-53 36.19856941503062 40.0 35.0 41.0 29.0 41.0 54-55 36.01273860969607 39.0 35.0 41.0 29.0 41.0 56-57 35.82986806708259 39.0 35.0 41.0 28.5 41.0 58-59 35.612819457958906 39.0 35.0 41.0 28.0 41.0 60-61 35.3738183990613 38.5 35.0 40.5 28.0 41.0 62-63 35.11335427279492 38.0 34.0 40.0 28.0 41.0 64-65 34.78339748726461 37.5 34.0 40.0 28.0 41.0 66-67 34.47659693205884 37.0 34.0 40.0 27.0 41.0 68-69 34.151993303188135 36.5 34.0 39.0 26.5 41.0 70-71 33.790129357220536 36.0 33.5 39.0 26.0 41.0 72-73 33.42716286989869 35.5 33.0 39.0 26.0 40.0 74-75 33.07547650392078 35.0 33.0 37.5 26.0 40.0 76-77 32.83602791769218 35.0 33.0 37.0 26.0 39.0 78-79 32.657414429626236 35.0 33.0 37.0 26.0 39.0 80-81 32.38377418293172 35.0 33.0 36.0 26.0 39.0 82-83 32.087584425619596 35.0 33.0 36.0 25.0 37.0 84-85 31.835906645297925 35.0 33.0 35.5 25.0 37.0 86-87 31.628740126495337 35.0 33.0 35.0 25.0 36.5 88-89 31.364958359567282 35.0 32.5 35.0 24.0 36.0 90-91 31.2425172428596 35.0 32.0 35.0 23.5 36.0 92-93 31.09268215900635 35.0 32.0 35.0 23.0 36.0 94-95 30.990092152710204 35.0 32.0 35.0 23.0 35.5 96-97 30.883307337874193 35.0 32.0 35.0 20.0 35.0 98-99 30.749213339247895 35.0 32.0 35.0 20.0 35.0 100-101 30.65461192833839 35.0 32.0 35.0 19.0 35.0 102-103 29.652632576841626 33.5 30.0 34.5 17.5 35.0 104-105 30.343435192604886 34.5 31.5 35.0 18.0 35.0 106-107 30.66493296033427 35.0 32.0 35.0 19.0 35.0 108-109 30.69357470951863 35.0 32.0 35.0 18.5 35.0 110-111 30.618868052773166 35.0 32.0 35.0 17.5 35.0 112-113 30.52684198099708 35.0 32.0 35.0 16.0 35.0 114-115 30.426078573063933 35.0 32.0 35.0 8.5 35.0 116-117 30.298087187339018 35.0 32.0 35.0 2.0 35.0 118-119 30.12356869955927 35.0 32.0 35.0 2.0 35.0 120-121 29.939631031423502 35.0 32.0 35.0 2.0 35.0 122-123 29.76041404327171 35.0 32.0 35.0 2.0 35.0 124-125 28.9719134565852 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21892044831957236 1101 2 0.17485364550681837 1101 3 0.19309823343823496 1101 4 0.17437775420305712 1101 5 0.12362975371802776 1101 6 0.19098231974686541 1101 7 0.28092856318168913 1101 8 0.07367142570380025 1101 9 0.3037111622518438 1101 10-11 0.33105168994894285 1101 12-13 0.29557681189076845 1101 14-15 0.41475082827746235 1101 16-17 0.36010389669331744 1101 18-19 0.15717482119223547 1101 20-21 0.2922236076318896 1101 22-23 0.4796407190409795 1101 24-25 0.47363879478146487 1101 26-27 0.5490564445051689 1101 28-29 0.6008294619836079 1101 30-31 0.6462315807204462 1101 32-33 0.5019653001325111 1101 34-35 0.5685494297341691 1101 36-37 0.4680352773668588 1101 38-39 0.5954673229551162 1101 40-41 0.6252631849545125 1101 42-43 0.6107689316796652 1101 44-45 0.5843418926523185 1101 46-47 0.6212837262018525 1101 48-49 0.5065790281637064 1101 50-51 0.5364394358268001 1101 52-53 0.4353872205097602 1101 54-55 0.5548330422153285 1101 56-57 0.5291276091560988 1101 58-59 0.5187501580065401 1101 60-61 0.5228073765075862 1101 62-63 0.6068813870315779 1101 64-65 0.5373446483392783 1101 66-67 0.5143756656702294 1101 68-69 0.505513695551322 1101 70-71 0.5045884712813873 1101 72-73 0.5848675627629731 1101 74-75 0.5445609900981268 1101 76-77 0.5193601828126333 1101 78-79 0.6239047796601511 1101 80-81 0.6203622652472802 1101 82-83 0.537237778952111 1101 84-85 0.5334189482624545 1101 86-87 0.5694272587183029 1101 88-89 0.6674886773098088 1101 90-91 0.5371037161651273 1101 92-93 0.4829092588171484 1101 94-95 0.4158044301784791 1101 96-97 0.37105111830557647 1101 98-99 0.33698734753578563 1101 100-101 0.3717192881156457 1101 102-103 0.09294290392276494 1101 104-105 0.2809322661129343 1101 106-107 0.24297893757355737 1101 108-109 0.18801629486627291 1101 110-111 0.1639694025804701 1101 112-113 0.23901774215003257 1101 114-115 0.24251786433724476 1101 116-117 0.22458479841570878 1101 118-119 0.22938957051177766 1101 120-121 0.05188002912259648 1101 122-123 0.011301020508120985 1101 124-125 -0.09118375238561072 1102 1 0.3309883713747652 1102 2 0.2980936386627313 1102 3 0.3104888898534881 1102 4 0.42470804164972265 1102 5 0.38053317531031894 1102 6 0.43082620028442165 1102 7 0.37391257039507764 1102 8 0.3555464849749015 1102 9 0.5383973904319248 1102 10-11 0.4878289675775562 1102 12-13 0.5097512237332467 1102 14-15 0.5160127195036281 1102 16-17 0.6025623542346352 1102 18-19 0.5768982879334317 1102 20-21 0.5451782565186463 1102 22-23 0.5626220217358124 1102 24-25 0.5215865468950298 1102 26-27 0.4420272552736719 1102 28-29 0.3967257439479752 1102 30-31 0.5346215191086046 1102 32-33 0.5662010026876558 1102 34-35 0.47011850208430417 1102 36-37 0.4157215341475933 1102 38-39 0.43502753010417905 1102 40-41 0.47988954241961324 1102 42-43 0.3444764653443926 1102 44-45 0.4019929906918662 1102 46-47 0.3756912384411777 1102 48-49 0.3364123766336462 1102 50-51 0.3523430084506529 1102 52-53 0.3071091207376213 1102 54-55 0.4093606772267506 1102 56-57 0.41828951448189855 1102 58-59 0.5403658594678404 1102 60-61 0.3954815030961143 1102 62-63 0.40608756818615177 1102 64-65 0.2101551047405863 1102 66-67 0.29729276740980026 1102 68-69 0.19948889125259228 1102 70-71 0.09244041620154064 1102 72-73 0.26232173773177436 1102 74-75 0.14580218347823148 1102 76-77 0.2563673294652773 1102 78-79 0.4061018472394977 1102 80-81 0.3929084916912515 1102 82-83 0.3809685393633728 1102 84-85 0.4653946617862097 1102 86-87 0.36167244063606674 1102 88-89 0.5646884970754762 1102 90-91 0.4268111665367087 1102 92-93 0.4242011355774089 1102 94-95 0.4103488542791034 1102 96-97 0.3661833612345653 1102 98-99 0.28793194705378866 1102 100-101 0.2891514405762301 1102 102-103 0.03467357501969204 1102 104-105 0.24113457313248787 1102 106-107 0.2331083229453732 1102 108-109 0.2897707948288897 1102 110-111 0.30660641354054263 1102 112-113 0.30693226732137546 1102 114-115 0.29013407458922913 1102 116-117 0.20065520320536834 1102 118-119 0.0895347263878854 1102 120-121 0.027564325001947054 1102 122-123 -0.0969174795026646 1102 124-125 -0.20931112667943808 1103 1 0.1492455575957763 1103 2 0.18324926533695063 1103 3 0.25197178494309114 1103 4 0.20070727523170717 1103 5 0.260875419573388 1103 6 0.2711753363684224 1103 7 0.2055538511152335 1103 8 0.12621718243262592 1103 9 0.18595204413153965 1103 10-11 0.3272345628746862 1103 12-13 0.27643882496614935 1103 14-15 0.30496753673546095 1103 16-17 0.3309104882320355 1103 18-19 0.3849236945917127 1103 20-21 0.3468547216680449 1103 22-23 0.2680953257079466 1103 24-25 0.2901052831667812 1103 26-27 0.39822953516923576 1103 28-29 0.40249566357558564 1103 30-31 0.5280828297279712 1103 32-33 0.46404671452190627 1103 34-35 0.46540191212957893 1103 36-37 0.3398368671866763 1103 38-39 0.3561382124018735 1103 40-41 0.35706090541830093 1103 42-43 0.29506552542033404 1103 44-45 0.282579815381915 1103 46-47 0.18383819265983448 1103 48-49 0.17355473655256048 1103 50-51 0.17198165324733594 1103 52-53 0.21875097577618874 1103 54-55 0.21923739433358946 1103 56-57 0.19325769798454928 1103 58-59 0.16007201143298744 1103 60-61 0.11214658956198065 1103 62-63 0.13962759013403314 1103 64-65 0.07002623957149012 1103 66-67 0.0100854129654806 1103 68-69 0.04935413684426493 1103 70-71 0.13463561465206908 1103 72-73 0.20643436751350208 1103 74-75 0.20108392007847442 1103 76-77 0.1527333049611883 1103 78-79 0.18852045060108935 1103 80-81 0.23434128245047248 1103 82-83 0.09589334202187416 1103 84-85 0.06911768088897574 1103 86-87 -0.02622246206523826 1103 88-89 0.030618790168478682 1103 90-91 0.027112906498697242 1103 92-93 0.052203589303310594 1103 94-95 0.011428894709137438 1103 96-97 -0.10906252502415015 1103 98-99 -0.14819728548105005 1103 100-101 -0.19976743224467342 1103 102-103 -0.29388260087126383 1103 104-105 -0.21963452833892916 1103 106-107 -0.2318948915048722 1103 108-109 -0.11838925483974805 1103 110-111 -0.13603662949546091 1103 112-113 -0.08402174734998624 1103 114-115 -0.12695328443973608 1103 116-117 -0.11941554193068882 1103 118-119 -0.18408875986845175 1103 120-121 -0.29428940649578195 1103 122-123 -0.298474154704067 1103 124-125 -0.4084769539417934 1104 1 0.4590163406330561 1104 2 0.41070401684374147 1104 3 0.410573619630064 1104 4 0.48460623582377593 1104 5 0.4665848520420113 1104 6 0.44289603766443975 1104 7 0.4108568730343265 1104 8 0.4356649091118783 1104 9 0.5516845708914104 1104 10-11 0.5129728961723075 1104 12-13 0.38494573241497676 1104 14-15 0.4688131506449693 1104 16-17 0.4072810583629263 1104 18-19 0.5061654271627418 1104 20-21 0.581772027952546 1104 22-23 0.5555682738585759 1104 24-25 0.537744228599685 1104 26-27 0.5829397157064022 1104 28-29 0.5950470247095083 1104 30-31 0.5059943221753471 1104 32-33 0.5552187757038851 1104 34-35 0.4992976350941518 1104 36-37 0.43517228186318846 1104 38-39 0.40542149586005394 1104 40-41 0.4521928793562253 1104 42-43 0.368805258314886 1104 44-45 0.3044714751828508 1104 46-47 0.3548595664169696 1104 48-49 0.31239279796010777 1104 50-51 0.2144487301076694 1104 52-53 0.110067941391641 1104 54-55 0.06823527209819957 1104 56-57 0.04959898583506117 1104 58-59 0.16217352600689594 1104 60-61 0.009900429564787316 1104 62-63 0.19627488676216842 1104 64-65 0.15254524091292865 1104 66-67 0.1497597241219637 1104 68-69 0.05794162001154746 1104 70-71 0.11182611688932553 1104 72-73 0.05464862169245066 1104 74-75 -0.0033793201344067825 1104 76-77 0.05009874739653242 1104 78-79 0.20073019140959047 1104 80-81 0.18982143202650548 1104 82-83 0.20809714914215505 1104 84-85 0.12075948767819966 1104 86-87 0.18105539489379296 1104 88-89 0.20200301366885043 1104 90-91 0.09667786590356187 1104 92-93 0.13534186048557828 1104 94-95 0.12947349956199972 1104 96-97 0.19140826133316935 1104 98-99 0.15036483116913502 1104 100-101 0.14070147511138487 1104 102-103 -0.05822899922847924 1104 104-105 0.0783164419721416 1104 106-107 0.15339620181720903 1104 108-109 0.13364744831831743 1104 110-111 0.1894074493783222 1104 112-113 0.0871311329493949 1104 114-115 0.13491379764789357 1104 116-117 0.060076289476274525 1104 118-119 -0.03703100977703144 1104 120-121 -0.12466704101213821 1104 122-123 -0.16576674698881178 1104 124-125 -0.2584840296295141 1105 1 0.18939642049706862 1105 2 0.029548376546422617 1105 3 0.002122969291793453 1105 4 0.13265454583099512 1105 5 0.10740925676975621 1105 6 0.2333462950517955 1105 7 0.21976344546479254 1105 8 0.25657358898358495 1105 9 0.26298402537381094 1105 10-11 0.1782545571348848 1105 12-13 0.24553570343428532 1105 14-15 0.43286151736675293 1105 16-17 0.3314359044312738 1105 18-19 0.16919189643571286 1105 20-21 0.22951786027594068 1105 22-23 0.19465950846490188 1105 24-25 0.2126476731768392 1105 26-27 0.2552699619218899 1105 28-29 0.10883541591307022 1105 30-31 0.20950508882799568 1105 32-33 0.35432723173310166 1105 34-35 0.1341335893380986 1105 36-37 0.09109311373082818 1105 38-39 0.1469868358785007 1105 40-41 0.2440553744415297 1105 42-43 0.16755508473905678 1105 44-45 0.14005662433878285 1105 46-47 0.06106956379954909 1105 48-49 0.039765317907544784 1105 50-51 0.0023353540898796155 1105 52-53 0.0798090326473968 1105 54-55 0.1364524294696423 1105 56-57 -0.021772581667761415 1105 58-59 -0.015471460166679663 1105 60-61 0.053616219372500495 1105 62-63 -0.0025696439844438146 1105 64-65 0.001333968577903022 1105 66-67 -0.06084542704598306 1105 68-69 -0.1018372652006505 1105 70-71 -0.05258322003863469 1105 72-73 -0.05280533767066231 1105 74-75 -0.054630270159655936 1105 76-77 -0.07991275530765307 1105 78-79 0.10911264923431929 1105 80-81 0.13564270507495735 1105 82-83 0.14910387319406482 1105 84-85 0.03872355630012336 1105 86-87 0.06063968162822775 1105 88-89 0.08926976095122185 1105 90-91 0.08917376366250451 1105 92-93 0.14169524219283502 1105 94-95 0.14266627529662657 1105 96-97 0.13083980583958876 1105 98-99 -0.011817671352073233 1105 100-101 -0.09653251500812843 1105 102-103 -0.18737481710315862 1105 104-105 -0.18832416300122645 1105 106-107 -0.14202929583622748 1105 108-109 -0.1464683799292743 1105 110-111 -0.1892037999557843 1105 112-113 -0.16210282656226127 1105 114-115 -0.23162962736723358 1105 116-117 -0.2850823683960293 1105 118-119 -0.3121099319212135 1105 120-121 -0.32949101993052565 1105 122-123 -0.3266558423307231 1105 124-125 -0.3215796591645699 1106 1 0.15534694119865833 1106 2 0.32032373929050806 1106 3 0.39326196987121165 1106 4 0.37645850382411084 1106 5 0.26079143939271177 1106 6 0.2820384197395711 1106 7 0.23145503255405941 1106 8 0.35340944741103186 1106 9 0.5281232917171081 1106 10-11 0.4732551608370912 1106 12-13 0.4247522935757928 1106 14-15 0.5438472787634012 1106 16-17 0.5292367420596591 1106 18-19 0.47713769679307916 1106 20-21 0.3453264940944578 1106 22-23 0.4822426692878068 1106 24-25 0.48880062635990384 1106 26-27 0.5122116942032306 1106 28-29 0.4635644278178077 1106 30-31 0.4815629848819398 1106 32-33 0.46574266721824387 1106 34-35 0.5215684215392358 1106 36-37 0.5909814794111341 1106 38-39 0.5446097331229893 1106 40-41 0.5086577168061126 1106 42-43 0.49529827049249064 1106 44-45 0.47561320067945445 1106 46-47 0.4229863419319031 1106 48-49 0.24249728963943795 1106 50-51 0.1663059338935753 1106 52-53 0.3622549911814943 1106 54-55 0.28931050413849846 1106 56-57 0.32252789610014077 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114-115 -0.8139430665308964 2216 116-117 -0.8707068133359108 2216 118-119 -0.6482460945113075 2216 120-121 -0.5583360621825229 2216 122-123 -0.6412159436633331 2216 124-125 -0.494514515358766 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 23736.0 3 4587.0 4 2847.0 5 2520.0 6 2629.0 7 3046.0 8 3348.0 9 3575.0 10 4006.0 11 4555.0 12 4861.0 13 5077.0 14 5249.0 15 4826.0 16 4563.0 17 3950.0 18 3834.0 19 3754.0 20 3602.0 21 3816.0 22 4643.0 23 6123.0 24 9023.0 25 13054.0 26 15787.0 27 15643.0 28 17796.0 29 22172.0 30 26124.0 31 31859.0 32 40616.0 33 53582.0 34 75610.0 35 114743.0 36 222330.0 37 430921.0 38 198545.0 39 728.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.479113651769886 18.654586607282823 12.607613529723775 24.25868621122352 2 15.676151796421752 20.068533926368602 39.440423138679236 24.814891138530406 3 18.381582214132447 24.962347484955092 29.534668575586576 27.12140172532589 4 12.288636454702088 16.400709219858157 37.4650827995581 33.84557152588166 5 13.010866422582465 38.99581488150275 34.70499494751426 13.288323748400524 6 32.271151846436695 37.07501468890649 17.308791945684323 13.345041518972486 7 28.603421346172148 32.88314490815569 21.15034161230706 17.3630921333651 8 26.442766901412984 35.555374302683234 19.9326168598083 18.069241936095484 9 26.439313719878655 16.302730238681242 19.514481140175146 37.74347490126495 10-11 24.622901475553927 27.07385854472255 27.954604724906336 20.348635254817186 12-13 25.047176780474057 24.605154941962276 27.82412281596101 22.523545461602662 14-15 22.126388466560538 26.357343445364624 24.8700162770315 26.646251811043342 16-17 22.499137855786525 29.466157441127354 25.863789799939614 22.170914903146503 18-19 22.572163360770478 28.343407769807026 27.04883344142917 22.035595427993325 20-21 23.067877864703604 27.626648312435005 26.989306203603903 22.31616761925749 22-23 23.000221796119284 27.540209490012018 26.845665674546392 22.613903039322306 24-25 22.71399748154084 27.814378112300382 26.880079846602946 22.591544559555835 26-27 22.457294394029535 27.959771893341784 26.756956798751077 22.82597691387761 28-29 22.577228198810026 27.902890226347164 26.89686307842447 22.62301849641834 30-31 22.59886662061411 27.59869311862492 26.86490491196652 22.937535348794448 32-33 22.282186799954495 27.85332376507591 26.812525354570475 23.05196408039912 34-35 23.05283078265971 27.820980632051917 26.42479984545708 22.70138873983129 36-37 23.194040401294146 27.365101260805417 26.453160169939 22.987698167961433 38-39 22.596656740679897 27.522362089122097 26.790398074520738 23.090583095677257 40-41 22.8002788924293 27.366337512069183 27.02848988478337 22.80489371071815 42-43 22.720797669837893 27.3460635585914 26.738767564040465 23.194371207530242 44-45 22.560505530130147 27.58668676213804 26.911737927960385 22.941069779771425 46-47 22.957882317756795 27.808888879349265 26.414648857141014 22.818579945752923 48-49 22.779999713811456 27.681765497109495 26.515833381031424 23.02240140804762 50-51 22.72605317383092 27.640804762177325 26.682144696926336 22.950997367065423 52-53 22.949386370287055 27.430496677677873 26.704250726389045 22.915866225646024 54-55 22.46563149753228 27.553257405666386 26.886493432528695 23.09461766427264 56-57 22.583662838056103 27.892787933705783 26.69891538003911 22.824633848198996 58-59 22.640693384046415 27.30278385337852 26.763349209790317 23.293173552784747 60-61 22.330116537307966 27.52879089681068 27.141322950294207 22.99976961558715 62-63 22.439198722443386 27.62970176036083 27.08973089460444 22.841368622591347 64-65 22.343496785687456 27.54066633038912 26.795310716879346 23.320526167044083 66-67 22.264531775273312 27.76768334805462 26.7341878479761 23.233597028695964 68-69 22.508187091405645 27.29426377710219 27.133813242016647 23.063735889475517 70-71 22.453547025364003 27.274389153221477 26.988015597610275 23.284048223804245 72-73 22.37125100705331 27.393996459751495 26.860419191752964 23.37433334144223 74-75 22.429140904682537 27.76709914245371 26.750464440144373 23.053295512719384 76-77 22.52586563713304 27.419775473999135 26.994312976266325 23.060045912601495 78-79 22.602548357219337 27.352035977649553 26.98330871454886 23.062106950582248 80-81 22.668523579223994 27.634965715923144 26.807833518941116 22.888677185911742 82-83 22.666052310649977 27.603378338011954 26.84157449239609 22.888994858941974 84-85 22.58900699807036 27.47921363468227 26.90389941552769 23.027879951719683 86-87 22.717311200944444 27.403999570707978 26.92065252387937 22.958036704468217 88-89 22.59869441356831 27.495591579765303 26.87166908925562 23.034044917410764 90-91 22.588346908287495 27.57957517929815 26.976143186670402 22.85593472574395 92-93 22.70768321559517 27.1533884944409 27.12759544768823 23.011332842275696 94-95 22.401764377602564 27.100618176094326 27.24754232073609 23.25007512556702 96-97 22.713888472522374 26.917946144074424 27.220627707400908 23.1475376760023 98-99 22.522143189585197 27.084754603125425 26.961799487645283 23.431302719644094 100-101 22.421175582541377 27.004055028181284 27.244387443901267 23.330381945376068 102-103 22.389065653729006 26.757144666300814 27.34563206408467 23.508157615885512 104-105 22.17578370805911 27.11458706350907 27.172111515873066 23.537517712558753 106-107 22.360664652459892 27.128181185591444 26.99745451488205 23.51369964706661 108-109 22.521400299360923 27.01675104676155 26.792659647234917 23.669189006642608 110-111 22.34099677852685 27.431496741858226 27.028964638921334 23.198541840693593 112-113 22.29678436737331 27.319211442709534 27.320499327080416 23.063504862836737 114-115 22.14709517702257 27.639311478518135 26.992503394132576 23.22108995032671 116-117 22.127916997728686 27.545981697114836 27.17124513066053 23.154856174495947 118-119 22.144715697397665 27.60517961510793 26.832389058098876 23.417715629395534 120-121 22.049218657479773 27.897024576412782 26.93326683356011 23.120489932547333 122-123 22.08324613042693 27.894984446579052 26.94381986294386 23.077949560050158 124-125 22.128575864639814 27.961039871668834 26.892662885991612 23.01772137769974 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1025.0 1 845.0 2 590.0 3 444.5 4 350.5 5 319.5 6 289.0 7 261.0 8 258.5 9 265.0 10 257.0 11 239.0 12 227.5 13 239.0 14 261.0 15 290.0 16 340.5 17 391.0 18 473.5 19 604.5 20 784.0 21 1083.0 22 1609.0 23 2316.0 24 3185.0 25 4282.0 26 5864.5 27 7934.0 28 9949.0 29 12232.5 30 15152.5 31 18558.0 32 22931.5 33 25596.5 34 27360.5 35 30203.0 36 34491.5 37 38741.0 38 40899.0 39 42381.0 40 43054.0 41 44914.5 42 46489.5 43 47803.5 44 50398.5 45 53356.0 46 53077.5 47 51213.5 48 50581.0 49 49562.5 50 49128.0 51 48301.0 52 48306.0 53 49286.5 54 50129.0 55 47770.0 56 42739.5 57 39056.0 58 36112.5 59 30782.0 60 24414.5 61 20050.0 62 17010.0 63 13395.0 64 9739.5 65 7362.0 66 5458.0 67 4472.0 68 3346.5 69 2099.5 70 1533.5 71 1107.5 72 787.0 73 541.5 74 386.5 75 323.0 76 224.5 77 116.5 78 63.5 79 47.0 80 27.0 81 15.5 82 13.5 83 10.5 84 11.0 85 9.0 86 5.0 87 5.0 88 5.0 89 4.0 90 2.5 91 2.5 92 4.0 93 3.0 94 1.5 95 2.5 96 3.0 97 1.5 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04772193921355389 2 0.008871844771335355 3 0.002217961192833839 4 0.006296147902237995 5 0.02489840306794116 6 0.026257798637742545 7 0.0030765268158662925 8 7.154713525270447E-5 9 0.0 10-11 1.0732070287905672E-4 12-13 8.227920554061015E-4 14-15 3.577356762635224E-4 16-17 1.4309427050540895E-4 18-19 3.577356762635224E-5 20-21 3.577356762635224E-5 22-23 0.0 24-25 0.0 26-27 3.577356762635224E-5 28-29 2.861885410108179E-4 30-31 1.0732070287905672E-4 32-33 2.1464140575811345E-4 34-35 7.154713525270448E-4 36-37 1.4309427050540895E-4 38-39 1.4309427050540895E-4 40-41 6.081506496479881E-4 42-43 6.439242172743404E-4 44-45 0.001323622002175033 46-47 7.154713525270447E-5 48-49 0.0 50-51 0.0 52-53 9.301127582851583E-4 54-55 0.0024326025985919523 56-57 1.0732070287905672E-4 58-59 9.301127582851583E-4 60-61 0.0011447541640432716 62-63 8.585656230324538E-4 64-65 0.0017886783813176122 66-67 0.0031838475187453497 68-69 0.0037919981683933375 70-71 0.0021464140575811344 72-73 0.0027187911396027702 74-75 0.0021106404899547825 76-77 0.0017529048136912598 78-79 0.0024683761662183047 80-81 0.0013951691374277373 82-83 7.51244920153397E-4 84-85 6.439242172743404E-4 86-87 0.0021464140575811344 88-89 0.005044073035315666 90-91 5.723770820216358E-4 92-93 0.001180527731669624 94-95 0.0014309427050540897 96-97 4.6505637914257914E-4 98-99 0.0011089805964169194 100-101 0.0023252818957128956 102-103 0.00207486692232843 104-105 9.658863259115106E-4 106-107 0.007691317039665731 108-109 0.0025756968690973615 110-111 0.0019675462194493733 112-113 0.0027903382748554746 114-115 0.003398488924503463 116-117 0.002253734760460191 118-119 0.04757884494304848 120-121 0.012985805048365863 122-123 0.005544902982084597 124-125 0.003291168221624406 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1397680.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.60934129318304 #Duplication Level Percentage of deduplicated Percentage of total 1 86.12233829276023 60.81041577480474 2 8.78372695279008 12.404263484713706 3 2.359668154045403 4.9984384208294115 4 0.9203168476590451 2.599318655768954 5 0.46288309769422065 1.6341935311968505 6 0.25743609806000545 1.0906435985462553 7 0.17252616984609223 0.8527371446067873 8 0.12465680868434799 0.7041548119129719 9 0.09838401393098983 0.6252147375701882 >10 0.6119027274801934 8.288857455596506 >50 0.060112542986660175 2.925869306626972 >100 0.02584792257021813 2.987512608801283 >500 2.0037149253790136E-4 0.07838046902526366 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 7.154713525270449E-5 0.0 8 0.0 0.0 0.0 7.154713525270449E-5 0.0 9 0.0 0.0 0.0 7.154713525270449E-5 0.0 10-11 0.0 0.0 0.0 1.0732070287905674E-4 0.0 12-13 0.0 0.0 0.0 1.4309427050540898E-4 0.0 14-15 0.0 0.0 0.0 1.4309427050540898E-4 0.0 16-17 0.0 0.0 0.0 1.4309427050540898E-4 0.0 18-19 3.5773567626352244E-5 0.0 0.0 1.4309427050540898E-4 0.0 20-21 7.154713525270449E-5 0.0 0.0 1.4309427050540898E-4 0.0 22-23 7.154713525270449E-5 0.0 0.0 1.4309427050540898E-4 0.0 24-25 7.154713525270449E-5 0.0 0.0 2.504149733844657E-4 0.0 26-27 7.154713525270449E-5 0.0 0.0 8.227920554061015E-4 0.0 28-29 7.154713525270449E-5 0.0 0.0 0.0041855074122832125 0.0 30-31 7.154713525270449E-5 0.0 0.0 0.013164672886497624 0.0 32-33 7.154713525270449E-5 0.0 0.0 0.031552286646442676 0.0 34-35 7.154713525270449E-5 0.0 0.0 0.06246064907561101 0.0 36-37 7.154713525270449E-5 0.0 0.0 0.11508356705397516 0.0 38-39 7.154713525270449E-5 0.0 0.0 0.19120971896285271 0.0 40-41 7.154713525270449E-5 0.0 0.0 0.27123518974300265 0.0 42-43 7.154713525270449E-5 0.0 0.0 0.3498297178180986 0.0 44-45 7.154713525270449E-5 0.0 0.0 0.44870785873733615 0.0 46-47 7.154713525270449E-5 0.0 0.0 0.5771707400835671 0.0 48-49 1.4309427050540898E-4 0.0 0.0 0.7071361112701047 0.0 50-51 1.4309427050540898E-4 0.0 0.0 0.851875965886326 0.0 52-53 1.4309427050540898E-4 0.0 0.0 0.983308053345544 0.0 54-55 1.4309427050540898E-4 0.0 0.0 1.1299796806135882 0.0 56-57 1.4309427050540898E-4 0.0 0.0 1.3040180871157918 0.0 58-59 1.4309427050540898E-4 0.0 0.0 1.4986978421384007 0.0 60-61 1.4309427050540898E-4 0.0 0.0 1.7129815122202507 0.0 62-63 1.4309427050540898E-4 0.0 0.0 1.9207186194264783 0.0 64-65 1.4309427050540898E-4 0.0 0.0 2.129600480796749 0.0 66-67 2.1464140575811345E-4 0.0 0.0 2.3757226260660524 0.0 68-69 2.8618854101081795E-4 0.0 0.0 2.651501058897602 0.0 70-71 2.8618854101081795E-4 0.0 0.0 2.941624692347318 0.0 72-73 3.219621086371702E-4 0.0 0.0 3.247560242687883 0.0 74-75 3.577356762635224E-4 0.0 0.0 3.5454825138801445 0.0 76-77 3.577356762635224E-4 0.0 0.0 3.864690057810085 0.0 78-79 3.577356762635224E-4 0.0 0.0 4.235661954095358 0.0 80-81 4.292828115162269E-4 0.0 0.0 4.640046362543643 0.0 82-83 4.292828115162269E-4 0.0 0.0 5.057058840364032 0.0 84-85 5.008299467689314E-4 0.0 0.0 5.460942418865549 0.0 86-87 5.723770820216359E-4 0.0 0.0 5.873375880029764 0.0 88-89 5.723770820216359E-4 0.0 0.0 6.3179697784900695 0.0 90-91 5.723770820216359E-4 0.0 0.0 6.825739797378513 0.0 92-93 5.723770820216359E-4 0.0 0.0 7.343132905958445 0.0 94-95 5.723770820216359E-4 0.0 0.0 7.8632805792456075 0.0 96-97 5.723770820216359E-4 0.0 0.0 8.370728636025413 0.0 98-99 6.796977849006926E-4 0.0 0.0 8.90987207372217 0.0 100-101 7.870184877797493E-4 0.0 0.0 9.511798122603171 0.0 102-103 9.301127582851583E-4 0.0 0.0 10.147172457214813 0.0 104-105 0.0012163012992959762 0.0 0.0 10.777610039494018 0.0 106-107 0.0012878484345486808 0.0 0.0 11.390590120771565 0.0 108-109 0.0012878484345486808 0.0 0.0 12.025070116192548 0.0 110-111 0.0012878484345486808 0.0 0.0 12.710885181157348 0.0 112-113 0.0012878484345486808 0.0 0.0 13.434763322076584 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1250 0.0 50.975155 1 GTATCAA 1985 0.0 48.600346 1 ATCAACG 2295 0.0 39.680386 3 TCAACGC 2325 0.0 39.16838 4 CAACGCA 2370 0.0 38.423306 5 AACGCAG 2425 0.0 37.299732 6 GTACATG 5700 0.0 36.983944 1 TATCAAC 2680 0.0 34.646305 2 ACATGGG 5800 0.0 34.48089 3 TACATGG 6155 0.0 33.74928 2 ACGCAGA 2860 0.0 31.617472 7 CGCAGAG 2915 0.0 30.816833 8 CATGGGA 3795 0.0 29.956339 4 CATGGGG 2555 0.0 27.256002 4 CGTACCC 90 0.009513602 26.45359 3 ATGGGAT 1800 0.0 25.46067 5 ATGGGAG 1860 0.0 23.679384 5 GTATAGA 380 0.0 23.506744 1 GCAGAGT 3820 0.0 22.73731 9 GAGTACA 4455 0.0 22.05571 1 >>END_MODULE