FastQCFastQC Report
Thu 26 May 2016
SRR1364389_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364389_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1397680
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39760.284471409764753No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38110.27266613244805676No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21570.15432717074008356No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27750.071.908261
GTATCAA48050.051.6939351
ATCAACG58850.041.988223
GTGGTAT12200.041.012511
TCAACGC60300.040.9785584
TATCAAC61200.040.278642
CAACGCA61600.040.2104075
AACGCAG63800.038.800236
GTACATG55250.036.548061
TGGTATC13400.034.658972
ACGCAGA73750.033.6413577
ACATGGG57350.032.807983
CGCAGAG75500.032.7827848
TACATGG59350.032.605322
TATAACG1203.6786794E-529.771172
GCAGAGT85550.028.4447929
CATGGGG26950.027.39614
GAGTACT57300.026.99716812-13
CATGGGA35300.026.8193514
CAGAGTA83150.026.15318510-11
Produced by FastQC (version 0.11.5)