FastQCFastQC Report
Thu 26 May 2016
SRR1364388_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364388_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1698570
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14250.046.7954521
GTATCAA23200.043.6276131
ATCAACG24350.040.5699963
CAACGCA24500.040.3216065
TCAACGC24650.040.076244
GTACATG57700.037.4568561
AACGCAG26500.037.2740756
TACATGG58750.036.0610162
TATCAAC28850.034.0356452
ACATGGG61000.033.852873
TAAGGTG11050.030.1593235
ACGCAGA33300.029.8350967
CGCAGAG33300.029.1204838
CATGGGG37350.028.839244
TAAGGTA7000.025.5046334
GTAAGGT12000.025.2920914
GGTAAGG11650.025.0302963
CATGGGA30800.023.3792464
AGGTAAG11600.022.5730652
ATAGTAC4800.022.3165533
Produced by FastQC (version 0.11.5)