FastQCFastQC Report
Thu 26 May 2016
SRR1364388_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364388_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1698570
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27340.16095892427159317No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27320.16084117816751736No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24650.072.478461
GTATCAA44950.050.4777451
CAACGCA56150.039.973145
ATCAACG56350.039.831273
TCAACGC56600.039.655344
TATCAAC58750.038.3054582
AACGCAG59000.038.1229326
ACGCAGA69000.032.4215747
CGCAGAG69800.031.7090268
TGGTATC12450.030.6046452
GTACATG56550.029.4869631
GTGGTAT13700.029.124531
TACATGG57700.028.5812172
ACATGGG59850.026.8581663
GCAGAGT82200.026.7085279
CAGAGTA76950.025.39932310-11
GAGTACT54000.024.95570412-13
GAGTACA44350.024.5732231
AGAGTAC73750.024.44420810-11
CATGGGG36400.023.8796624
Produced by FastQC (version 0.11.5)