FastQCFastQC Report
Thu 26 May 2016
SRR1364387_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364387_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1749300
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13900.052.2565731
GTATCAA21350.044.3399471
ATCAACG22400.039.3253863
TCAACGC23800.037.0121274
CAACGCA24350.036.6649935
GTACATG58250.035.978471
AACGCAG25250.035.3530656
TACATGG60200.034.208882
TATCAAC27600.032.5632062
ACATGGG61050.032.1727033
ACGCAGA30750.029.0214547
CGCAGAG32600.026.8262778
CATGGGG43750.026.5286944
GAGTACA46700.023.71331
CATGGGA25350.023.2443034
GTATAGG6350.022.502641
GTACGGG1350.002546295822.0511971
AGTACAT49050.021.842022
GTATAAC4800.021.0864541
GCAGAGT43050.020.7290179
Produced by FastQC (version 0.11.5)