FastQCFastQC Report
Thu 26 May 2016
SRR1364387_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364387_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1749300
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22960.13125250100040017No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21220.12130566512319213No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21600.064.806261
GTATCAA38750.051.650011
ATCAACG47200.041.2569163
TCAACGC48750.039.9451564
CAACGCA48900.039.944415
AACGCAG50500.038.533366
TATCAAC50850.038.2955062
GTACATG53200.034.150031
ACGCAGA58350.033.4446457
CGCAGAG59600.032.6433758
TACATGG56250.031.7606772
ACATGGG57950.030.0068533
TAAGGTG9100.026.830795
GCAGAGT72400.026.7899959
CATGGGG41650.025.5934624
GTGGTAT12950.025.2985571
AGAGTAC66000.024.339610-11
GGTAAGG9100.024.2131523
CAGAGTA68050.023.78123510-11
GTAAGGT9650.023.450244
Produced by FastQC (version 0.11.5)