FastQCFastQC Report
Thu 26 May 2016
SRR1364386_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364386_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2425352
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17750.059.704021
GTATCAA28150.046.317771
TAAGGTA10450.043.854384
ATCAACG31950.040.0503353
TCAACGC31850.039.989224
CAACGCA32100.039.676965
GTACATG80600.035.3821
TATCAAC36950.035.27512
AACGCAG36300.035.0833446
TAAGGTG21250.034.7290085
AGGTAAG18750.034.5990222
TACATGG80550.034.4317362
TGTAAGG12800.033.94312
GTGTAAG14350.033.1909751
ACATGGG84500.031.7656973
GTAAGGT23900.030.3808944
ACGCAGA42350.030.0646177
GGTAAGG22800.030.019373
CGCAGAG44300.028.7394528
CATGGGA38900.027.3868474
Produced by FastQC (version 0.11.5)