##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364386_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2425352 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.33806103196567 31.0 31.0 34.0 28.0 34.0 2 31.42684773179316 33.0 31.0 34.0 28.0 34.0 3 31.583051037540116 34.0 31.0 34.0 28.0 34.0 4 35.03914483340975 37.0 35.0 37.0 32.0 37.0 5 35.13217297942732 37.0 35.0 37.0 33.0 37.0 6 35.18690235479221 37.0 35.0 37.0 33.0 37.0 7 35.102578100003626 37.0 35.0 37.0 32.0 37.0 8 35.072772941824525 37.0 35.0 37.0 32.0 37.0 9 36.66889136092411 39.0 37.0 39.0 33.0 39.0 10-11 36.58154321517041 39.0 37.0 39.0 32.0 39.0 12-13 36.61295226424865 39.0 37.0 39.0 32.0 39.0 14-15 37.97341540526901 40.0 38.0 41.0 33.0 41.0 16-17 37.97881771388236 40.0 38.0 41.0 33.0 41.0 18-19 37.98081144510158 40.0 38.0 41.0 33.0 41.0 20-21 37.99825324324057 40.0 38.0 41.0 33.0 41.0 22-23 37.97326346856044 40.0 38.0 41.0 32.5 41.0 24-25 37.97227969383413 40.0 38.0 41.0 32.5 41.0 26-27 37.92942014190105 40.0 38.0 41.0 32.0 41.0 28-29 37.93410420425572 40.0 38.0 41.0 32.0 41.0 30-31 37.82573168760658 40.0 38.0 41.0 32.0 41.0 32-33 37.78234252182776 40.0 38.0 41.0 32.0 41.0 34-35 37.78473619499355 40.0 38.0 41.0 32.0 41.0 36-37 37.723614757775366 40.0 38.0 41.0 32.0 41.0 38-39 37.62432484027061 40.0 38.0 41.0 32.0 41.0 40-41 37.56179948312658 40.0 38.0 41.0 32.0 41.0 42-43 37.423128684001334 40.0 37.0 41.0 31.0 41.0 44-45 37.29363634639426 40.0 37.0 41.0 31.0 41.0 46-47 37.18222200323912 40.0 37.0 41.0 31.0 41.0 48-49 37.019380279646 40.0 36.5 41.0 31.0 41.0 50-51 36.885335819295506 40.0 36.0 41.0 30.5 41.0 52-53 36.704868200574595 40.0 36.0 41.0 30.0 41.0 54-55 36.51055187040892 39.0 35.0 41.0 30.0 41.0 56-57 36.30033454937676 39.0 35.0 41.0 30.0 41.0 58-59 36.06281706737826 39.0 35.0 41.0 30.0 41.0 60-61 35.798292165425885 38.5 35.0 40.5 29.0 41.0 62-63 35.51723873483107 38.0 35.0 40.0 29.0 41.0 64-65 35.142325732512234 37.0 34.0 40.0 29.0 41.0 66-67 34.82746504424925 37.0 34.0 39.5 28.5 41.0 68-69 34.485959976118934 36.0 34.0 39.0 28.0 41.0 70-71 34.098822768818714 35.5 34.0 39.0 28.0 41.0 72-73 33.74801080420491 35.0 34.0 38.0 28.0 40.0 74-75 33.394999571196266 35.0 33.0 37.0 27.0 39.5 76-77 33.16707533586877 35.0 33.0 37.0 27.5 39.0 78-79 33.00178675095409 35.0 33.0 36.0 27.0 39.0 80-81 32.761291350698784 35.0 33.0 36.0 27.0 38.0 82-83 32.4713311717227 35.0 33.0 36.0 26.5 37.0 84-85 32.23891604187763 35.0 33.0 35.0 26.0 37.0 86-87 32.06225467478535 35.0 33.0 35.0 26.0 36.0 88-89 31.795338779690535 35.0 33.0 35.0 25.0 36.0 90-91 31.675080359469472 35.0 33.0 35.0 25.0 36.0 92-93 31.530874281341433 35.0 33.0 35.0 25.0 36.0 94-95 31.42122195046327 35.0 33.0 35.0 25.0 35.0 96-97 31.31285726772856 35.0 33.0 35.0 24.0 35.0 98-99 31.170157568880725 35.0 32.5 35.0 24.0 35.0 100-101 31.06521754368026 35.0 32.0 35.0 23.0 35.0 102-103 30.022581258308072 33.5 30.5 34.5 19.5 35.0 104-105 30.724678314735343 34.5 32.0 35.0 20.0 35.0 106-107 31.060526884345034 35.0 33.0 35.0 23.0 35.0 108-109 31.102916607568716 35.0 33.0 35.0 23.0 35.0 110-111 31.008733783797155 35.0 33.0 35.0 22.0 35.0 112-113 30.897967800137877 35.0 33.0 35.0 19.5 35.0 114-115 30.7743003902114 35.0 33.0 35.0 19.0 35.0 116-117 30.62633341469609 35.0 33.0 35.0 17.0 35.0 118-119 30.421185254758896 35.0 32.0 35.0 6.0 35.0 120-121 30.22087659853085 35.0 32.0 35.0 2.0 35.0 122-123 30.02021809617738 35.0 32.0 35.0 2.0 35.0 124-125 29.183796413881367 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23607004520368235 1101 2 0.19415175000830942 1101 3 0.27666551052751487 1101 4 0.3077874431850205 1101 5 0.3103919534960369 1101 6 0.3112192385041155 1101 7 0.34186168697444685 1101 8 0.28912853336185407 1101 9 0.4555525914259846 1101 10-11 0.46253948917708243 1101 12-13 0.5508080655273133 1101 14-15 0.6127979098740681 1101 16-17 0.527947465565191 1101 18-19 0.449302228541427 1101 20-21 0.5193829706776114 1101 22-23 0.6090025190494401 1101 24-25 0.6076670317682513 1101 26-27 0.6971885027587703 1101 28-29 0.6068163477131918 1101 30-31 0.6405969352384631 1101 32-33 0.5993342987868502 1101 34-35 0.6286238644539139 1101 36-37 0.6236433853743932 1101 38-39 0.7012581439868768 1101 40-41 0.643848516014657 1101 42-43 0.7370365453654628 1101 44-45 0.666229385679074 1101 46-47 0.7103603673078496 1101 48-49 0.6663930150550428 1101 50-51 0.5778907296389875 1101 52-53 0.5798947784649187 1101 54-55 0.6508407697587799 1101 56-57 0.5761477755464526 1101 58-59 0.5699821028197576 1101 60-61 0.5770592079844619 1101 62-63 0.6490907618889423 1101 64-65 0.6128634802370954 1101 66-67 0.6092741427552397 1101 68-69 0.4867504319783791 1101 70-71 0.5112451385595733 1101 72-73 0.5504976974077849 1101 74-75 0.5475540040087523 1101 76-77 0.6246716088953548 1101 78-79 0.5824064802728799 1101 80-81 0.5152520601327026 1101 82-83 0.46211703651564306 1101 84-85 0.5131096573565728 1101 86-87 0.5175458058680533 1101 88-89 0.5589318572245041 1101 90-91 0.488845390593422 1101 92-93 0.5235278581884018 1101 94-95 0.47481373675114824 1101 96-97 0.4654104368193295 1101 98-99 0.400019694447753 1101 100-101 0.4065264941975322 1101 102-103 0.23410549795787716 1101 104-105 0.3164253047118919 1101 106-107 0.3261514606381155 1101 108-109 0.3695342870171814 1101 110-111 0.35440834643812735 1101 112-113 0.36318343308701273 1101 114-115 0.25845209802474756 1101 116-117 0.2383435287068707 1101 118-119 0.2859785196807607 1101 120-121 0.13325572543585196 1101 122-123 0.17961092582391558 1101 124-125 0.12957723633678242 1102 1 0.08459111141176479 1102 2 0.13795664944013808 1102 3 0.20959589678347967 1102 4 0.23319755563928624 1102 5 0.25881093415794965 1102 6 0.28059871686467375 1102 7 0.23794264730354797 1102 8 0.2580816296476627 1102 9 0.403944090760028 1102 10-11 0.3900710168354422 1102 12-13 0.3114915160356375 1102 14-15 0.45240394059245403 1102 16-17 0.49612649039954704 1102 18-19 0.4381909628829703 1102 20-21 0.4204205594018404 1102 22-23 0.3807407973358181 1102 24-25 0.4036362965162752 1102 26-27 0.35546329074791316 1102 28-29 0.3190721769580449 1102 30-31 0.4046609684183764 1102 32-33 0.4333968145928253 1102 34-35 0.3244389050015144 1102 36-37 0.21879567070813266 1102 38-39 0.2837130442616882 1102 40-41 0.21501120378967897 1102 42-43 0.14644771998147377 1102 44-45 0.21937995975221725 1102 46-47 0.20829467344646702 1102 48-49 0.1791664795569261 1102 50-51 0.16244892092925767 1102 52-53 0.2482778479437826 1102 54-55 0.17068203558582695 1102 56-57 0.15134132502127073 1102 58-59 0.2534332433791633 1102 60-61 0.2314110364200488 1102 62-63 0.2815784964496686 1102 64-65 0.29685126537695083 1102 66-67 0.318040897092402 1102 68-69 0.2729355853174411 1102 70-71 0.19400671696565297 1102 72-73 0.2410627084509045 1102 74-75 0.20564017398834977 1102 76-77 0.20073461363293177 1102 78-79 0.35245807098655035 1102 80-81 0.2978579812629789 1102 82-83 0.2404374721705551 1102 84-85 0.21426134417059473 1102 86-87 0.13168254654885203 1102 88-89 0.1590924038163095 1102 90-91 0.07187701941004576 1102 92-93 0.1635769620948011 1102 94-95 0.03064504691923986 1102 96-97 0.0028944622437734324 1102 98-99 0.003989102503322073 1102 100-101 0.031529697542499235 1102 102-103 -0.2625702308030995 1102 104-105 -0.16669281441882333 1102 106-107 -0.19290932704755193 1102 108-109 -0.0945465215745287 1102 110-111 -0.06789435280150258 1102 112-113 -0.16038458741439499 1102 114-115 -0.27134377303218926 1102 116-117 -0.2581107056093863 1102 118-119 -0.33250043940072516 1102 120-121 -0.4327724639998536 1102 122-123 -0.3497705335526149 1102 124-125 -0.4070647308542199 1103 1 0.29368364909050726 1103 2 0.3011890547844658 1103 3 0.35846871480436704 1103 4 0.4858441988967357 1103 5 0.3976828601416358 1103 6 0.3992486754861133 1103 7 0.2649447527243254 1103 8 0.3122589235573727 1103 9 0.4436835700249091 1103 10-11 0.5488019927495387 1103 12-13 0.4539567382573395 1103 14-15 0.5729844757836489 1103 16-17 0.47840099850738227 1103 18-19 0.47233624666424845 1103 20-21 0.49358681883590805 1103 22-23 0.5233854099594524 1103 24-25 0.554770826129662 1103 26-27 0.6031948827328861 1103 28-29 0.5101707514924243 1103 30-31 0.6966823714135089 1103 32-33 0.6437125244983974 1103 34-35 0.6464079792430084 1103 36-37 0.5705163154552508 1103 38-39 0.5162207803703893 1103 40-41 0.5354881021148898 1103 42-43 0.42293596200550354 1103 44-45 0.4211731511246626 1103 46-47 0.46773562578506045 1103 48-49 0.48329124648251565 1103 50-51 0.4615953175044254 1103 52-53 0.43725326744495874 1103 54-55 0.42212659932352636 1103 56-57 0.408325120334041 1103 58-59 0.410506496343956 1103 60-61 0.2728272425489564 1103 62-63 0.30665334279441936 1103 64-65 0.3714153774178044 1103 66-67 0.3109957691022629 1103 68-69 0.36557295269858514 1103 70-71 0.4100231507647436 1103 72-73 0.43833804675129784 1103 74-75 0.38891061009788785 1103 76-77 0.3669639445206556 1103 78-79 0.4522578663831993 1103 80-81 0.4678041338344343 1103 82-83 0.4876843414599108 1103 84-85 0.3527749398742728 1103 86-87 0.31252281610627364 1103 88-89 0.464927645937248 1103 90-91 0.2937191360332996 1103 92-93 0.2571098480006242 1103 94-95 0.24331982252355644 1103 96-97 0.22623100740252156 1103 98-99 0.18743143393634654 1103 100-101 0.1986211262772848 1103 102-103 -0.06935603727067274 1103 104-105 0.12243147454192638 1103 106-107 0.12063429607009013 1103 108-109 0.08479284828678502 1103 110-111 0.10292237565655427 1103 112-113 0.15035808803044048 1103 114-115 0.04337035197965733 1103 116-117 0.008399006742610737 1103 118-119 -0.07606050827680733 1103 120-121 -0.19345997484826682 1103 122-123 -0.19928602792757744 1103 124-125 -0.17712207270949776 1104 1 0.27020995346056154 1104 2 0.31128949960356067 1104 3 0.2777751975215814 1104 4 0.4090286736221458 1104 5 0.36500609272971474 1104 6 0.3423774953848522 1104 7 0.380532086326852 1104 8 0.3803130989312038 1104 9 0.532985133250051 1104 10-11 0.5633823790853469 1104 12-13 0.46113355122618316 1104 14-15 0.536824488395375 1104 16-17 0.5510296296234785 1104 18-19 0.43473699648529873 1104 20-21 0.5382385427229437 1104 22-23 0.526637772692176 1104 24-25 0.5729075201654439 1104 26-27 0.5482152161964464 1104 28-29 0.5572558377293859 1104 30-31 0.6239189131121847 1104 32-33 0.6315793068818323 1104 34-35 0.5178572022844534 1104 36-37 0.5011875704796296 1104 38-39 0.42561256924322777 1104 40-41 0.5121199002564296 1104 42-43 0.4169771821706405 1104 44-45 0.4510435384043916 1104 46-47 0.352910960939802 1104 48-49 0.3139820598472767 1104 50-51 0.2821675779930004 1104 52-53 0.253288599295999 1104 54-55 0.25403052960766814 1104 56-57 0.12424309394904043 1104 58-59 0.2652853273205622 1104 60-61 0.15774660679718977 1104 62-63 0.20426379211021128 1104 64-65 0.3457216831512895 1104 66-67 0.31694143903155236 1104 68-69 0.30183070720773486 1104 70-71 0.06666034363242801 1104 72-73 0.11348913021944895 1104 74-75 0.04053801642105981 1104 76-77 0.12616074812902411 1104 78-79 0.27257849862149897 1104 80-81 0.15482066509762404 1104 82-83 0.18287965119453986 1104 84-85 0.17813293042727452 1104 86-87 0.20614469955396686 1104 88-89 0.2749147228478144 1104 90-91 0.23551734148387382 1104 92-93 0.18801011668813672 1104 94-95 0.15234375563943914 1104 96-97 0.0518779894367043 1104 98-99 0.09536339385412163 1104 100-101 0.10596625404642523 1104 102-103 -0.10838606977012688 1104 104-105 -0.023606400630796998 1104 106-107 -0.024219222038624366 1104 108-109 -0.003219355724318973 1104 110-111 -0.01457404697795539 1104 112-113 -0.0019066235778559815 1104 114-115 -0.06691959652463453 1104 116-117 -0.05630501419652845 1104 118-119 -0.05049881437212633 1104 120-121 -0.10369718875735146 1104 122-123 -0.24822366672870189 1104 124-125 -0.3821813983097364 1105 1 0.06751436714414538 1105 2 0.09183966751268713 1105 3 0.07935699505958738 1105 4 0.20217423869704731 1105 5 0.1706601291235117 1105 6 0.18651692317886415 1105 7 0.25450193601783866 1105 8 0.2895789516769227 1105 9 0.2779168822360205 1105 10-11 0.2674430172917468 1105 12-13 0.3043947118727388 1105 14-15 0.38190145095973804 1105 16-17 0.35974313973342475 1105 18-19 0.30288044373047285 1105 20-21 0.3121161245786439 1105 22-23 0.3183035437592139 1105 24-25 0.2990040186026732 1105 26-27 0.2991508544028463 1105 28-29 0.3617787317847885 1105 30-31 0.37344050486036195 1105 32-33 0.42768730481836315 1105 34-35 0.30572889720571084 1105 36-37 0.24411828647043876 1105 38-39 0.29572227914479754 1105 40-41 0.30368167640119736 1105 42-43 0.19527897353848545 1105 44-45 0.21416602229624004 1105 46-47 0.22824949760164515 1105 48-49 0.19697038712846648 1105 50-51 0.25618212337398205 1105 52-53 0.32121814573905283 1105 54-55 0.29733113878400275 1105 56-57 0.33025245159548433 1105 58-59 0.2809223388921325 1105 60-61 0.22627673181868602 1105 62-63 0.12478211671091799 1105 64-65 0.16938643892442684 1105 66-67 0.06640155049893792 1105 68-69 0.14234580132787755 1105 70-71 0.1203013354595015 1105 72-73 0.018833678988954716 1105 74-75 -0.008717803501461674 1105 76-77 -0.05703926729636066 1105 78-79 0.09892246704244911 1105 80-81 0.03905645730502272 1105 82-83 -0.007254518059880866 1105 84-85 0.0042864522200503075 1105 86-87 0.07086816788893202 1105 88-89 0.08994505522696628 1105 90-91 0.005569237794933457 1105 92-93 -0.0030369320055108062 1105 94-95 6.942778169261032E-5 1105 96-97 -0.07380935694161295 1105 98-99 -0.092119062929509 1105 100-101 -0.17387949840898997 1105 102-103 -0.29865489506371645 1105 104-105 -0.14129925589515224 1105 106-107 -0.1604840215591743 1105 108-109 -0.09367344370465958 1105 110-111 -0.16075281835855293 1105 112-113 -0.18441660582601926 1105 114-115 -0.2911569881676144 1105 116-117 -0.34710105265378743 1105 118-119 -0.31965916758819546 1105 120-121 -0.44826966531582 1105 122-123 -0.5554404718023918 1105 124-125 -0.7168045004814267 1106 1 0.23036756586483875 1106 2 0.21495875049445345 1106 3 0.2260902390966315 1106 4 0.28577318293546483 1106 5 0.2473430149123388 1106 6 0.20117791619006198 1106 7 0.22041459574126065 1106 8 0.24915203036395894 1106 9 0.2666391252130538 1106 10-11 0.41337282251041785 1106 12-13 0.35332346011490046 1106 14-15 0.40537893401926084 1106 16-17 0.48970690487811197 1106 18-19 0.36138333371076214 1106 20-21 0.3274930824909319 1106 22-23 0.3504929582003129 1106 24-25 0.3248644065859452 1106 26-27 0.39542501223859716 1106 28-29 0.28391244234939705 1106 30-31 0.4187625485912392 1106 32-33 0.32906205765898733 1106 34-35 0.26117004821666967 1106 36-37 0.3167032622176862 1106 38-39 0.29120021172306565 1106 40-41 0.31144817571324523 1106 42-43 0.2715017860071711 1106 44-45 0.18618401215776714 1106 46-47 0.2372348609069448 1106 48-49 0.21583840478118788 1106 50-51 0.1936904379520641 1106 52-53 0.21085977603419792 1106 54-55 0.1398401215656193 1106 56-57 0.11873163553672583 1106 58-59 0.14738649432842976 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-0.4760944201390416 2216 110-111 -0.5244199599803139 2216 112-113 -0.517919922760175 2216 114-115 -0.4781477679536792 2216 116-117 -0.5682730236212556 2216 118-119 -0.5031195880938206 2216 120-121 -0.3477159973385291 2216 122-123 -0.4290678669203416 2216 124-125 -0.3679608138483239 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 25329.0 3 4923.0 4 3278.0 5 2814.0 6 3275.0 7 3905.0 8 4462.0 9 5055.0 10 5612.0 11 6457.0 12 7311.0 13 7474.0 14 7640.0 15 7357.0 16 6968.0 17 5851.0 18 5711.0 19 5910.0 20 6328.0 21 6833.0 22 8338.0 23 10355.0 24 14470.0 25 19889.0 26 23427.0 27 25727.0 28 30676.0 29 37690.0 30 45130.0 31 56707.0 32 73283.0 33 98384.0 34 138509.0 35 211971.0 36 425591.0 37 773442.0 38 298672.0 39 598.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.096160303473056 18.19398713473547 12.709873187135395 24.99997937465607 2 15.852782542719535 19.936291812363923 38.55186547469816 25.65906017021838 3 18.256223590765302 24.20858883563519 29.91446788999904 27.620719683600463 4 12.521420878408579 16.29631981474548 37.60846510231717 33.573794204528774 5 13.082039636970421 38.49402772954742 35.13997160892908 13.28396102455308 6 32.90340781141348 37.033975829474976 17.016496700907283 13.046119658204262 7 29.064000613542973 32.37218473251093 21.232380225114195 17.3314344288319 8 27.250552394727524 33.89301910776552 20.5023689374189 18.354059560088054 9 27.152058752708886 15.298439154399032 19.41982029824949 38.12968179464259 10-11 24.695822440582322 26.173422697699696 28.344221445839914 20.78653341587807 12-13 25.757478613757083 23.615669191749998 27.607637303338823 23.0192148911541 14-15 22.490236949941316 25.30190053491392 24.705263398772175 27.50259911637259 16-17 23.016873035591992 28.457780715270253 26.04274683767161 22.482599411466143 18-19 23.35212992926177 27.198275053440728 26.974925892154555 22.474669125142942 20-21 23.305632193931476 26.698171378457925 26.98722226448414 23.008974163126457 22-23 23.479359589733694 26.67652503152636 26.8780215981065 22.966093780633447 24-25 23.272848642176474 26.796873196138126 26.845958854632237 23.08431930705316 26-27 22.515925536520502 27.233553920052778 26.535902034757868 23.714618508668853 28-29 23.23685763209575 27.48852430357273 26.332605326339408 22.94201273799211 30-31 22.90458291217358 26.852183801366113 26.27150388614575 23.971729400314555 32-33 22.44041991482463 27.165925398777663 26.240677114922427 24.152977571475283 34-35 23.87355135842199 27.039076085443952 25.88983160040943 23.197540955724627 36-37 23.97768899794071 26.222045019884753 25.95622776996562 23.844038212208915 38-39 22.907464375200746 26.79907246262081 26.131972043587993 24.161491118590455 40-41 23.581217785404668 26.394352619862726 26.524417019160634 23.500012575571972 42-43 23.271034607448346 26.41215290039852 26.168639696734115 24.148172795419015 44-45 23.027971435445355 26.7839394783396 26.707784375092125 23.480304711122912 46-47 24.02673345563868 27.207160367130438 25.48029655919835 23.285809618032527 48-49 23.13641483792868 27.180508231382493 25.660646372155465 24.022430558533358 50-51 23.422971181090414 27.022325006844365 26.085285764705496 23.469418047359724 52-53 23.84971578030074 26.53952079402158 26.217666955493996 23.39309647018368 54-55 22.884148903403613 27.010935245747188 26.481861659513534 23.623054191335672 56-57 23.056342383573504 27.232213907271113 26.27548601232812 23.435957696827263 58-59 22.875263468649294 26.427822784142073 26.331629926740046 24.365283820468587 60-61 22.262922246141045 26.965431138734353 26.804278010483507 23.967368604641095 62-63 22.759318922672094 26.953194900166327 26.99706514649116 23.290421030670416 64-65 22.492111340496933 27.00065599672125 26.494227352548137 24.013005310233673 66-67 22.325243290894353 27.12080142300743 26.50598733510192 24.047967950996295 68-69 23.27198763685063 26.307688374747247 27.00130129733902 23.419022691063105 70-71 23.04514591478795 26.380461818680352 26.316490417174766 24.257901849356937 72-73 22.805164989513628 26.69210142042778 26.324124883184098 24.178608706874495 74-75 22.880776413848242 27.24773322277677 26.30090365972603 23.570586703648967 76-77 23.206901193842707 26.641087906150098 26.529741751550674 23.622269148456525 78-79 23.22697784268629 26.64656326202102 26.685919315348983 23.44053957994371 80-81 23.56568825564553 26.800633481588253 26.247883275121254 23.385794987644964 82-83 23.356894983155964 26.838428187064125 26.411560113542865 23.393116716237046 84-85 22.97855286412952 27.100023893601527 26.57370495752031 23.347718284748645 86-87 23.571520607265704 27.197426295124615 25.983059810188237 23.24799328742144 88-89 23.241088286484413 27.236891531320833 26.02186122669117 23.500158955503593 90-91 22.86477655171703 27.165002808887422 26.37548382441619 23.59473681497936 92-93 23.285314250219713 26.511382722407202 26.781718127562936 23.42158489981015 94-95 22.78128612919346 26.302898087018477 27.293569848811956 23.62224593497611 96-97 23.33556532003927 25.867516470866182 27.06959386775396 23.727324341340587 98-99 22.915842896624916 26.380109453011197 26.622591619377626 24.08145603098626 100-101 22.835633445030073 26.114112093388204 27.15651191163877 23.893742549942957 102-103 22.58914753293329 25.706963556760254 27.4470229592431 24.256865951063354 104-105 22.38491226674094 26.383907044574677 26.802467865772744 24.428712822911645 106-107 22.73250573362813 26.337413603852273 26.3437018331941 24.586378829325497 108-109 22.878759016402906 26.002926645836272 26.666575956313892 24.45173838144693 110-111 22.860857833900543 26.311807358870055 26.900348737773765 23.926986069455634 112-113 22.841955916345125 26.586794694919963 27.185587090741016 23.385662297993896 114-115 22.797631785434053 26.9277615391498 26.657752257774032 23.61685441764212 116-117 22.794264232473616 26.687234505315306 26.955654076854174 23.562847185356908 118-119 22.411090794900115 27.154358858538323 26.507230817938904 23.927319528622654 120-121 22.437198626912927 27.387577928918066 26.77105366845543 23.40416977571358 122-123 22.549451745660125 27.450280239959458 26.612013723985594 23.38825429039482 124-125 22.471143609454717 27.38176033671905 26.777233695325002 23.369862358501226 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 398.0 1 352.5 2 280.0 3 227.5 4 193.0 5 175.5 6 163.5 7 151.5 8 149.0 9 151.0 10 141.0 11 136.0 12 142.0 13 140.0 14 164.5 15 188.5 16 200.5 17 258.0 18 335.0 19 419.5 20 524.5 21 724.0 22 1052.5 23 1657.0 24 2540.5 25 3943.0 26 6479.0 27 10015.5 28 13637.5 29 17678.0 30 22398.0 31 29663.0 32 43212.0 33 48610.5 34 44852.0 35 46634.5 36 53705.5 37 60405.0 38 62560.5 39 65200.5 40 69737.0 41 75660.0 42 77894.5 43 77910.0 44 79480.5 45 84875.0 46 87092.0 47 86632.5 48 85752.0 49 83080.0 50 85060.5 51 86596.0 52 88486.0 53 93169.5 54 99077.5 55 96097.0 56 87855.5 57 80836.5 58 75039.5 59 64966.0 60 48863.0 61 40235.5 62 36909.0 63 27532.0 64 17727.0 65 13111.5 66 9496.0 67 7283.0 68 5609.0 69 4161.0 70 3047.5 71 1948.0 72 1335.0 73 1028.0 74 719.0 75 435.0 76 310.0 77 198.5 78 115.5 79 82.0 80 53.5 81 32.0 82 26.5 83 26.5 84 18.5 85 16.0 86 14.0 87 7.0 88 3.0 89 5.0 90 8.0 91 5.5 92 1.5 93 4.0 94 6.0 95 3.5 96 2.5 97 2.0 98 1.0 99 3.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.047415797789351816 2 0.009565621814895323 3 0.0026387922247987096 4 0.006102207019847016 5 0.026635309019061977 6 0.03075842187030996 7 0.003875726080173105 8 1.236933855374395E-4 9 0.0 10-11 2.061556425623992E-5 12-13 8.040070059933569E-4 14-15 3.504645923560786E-4 16-17 1.236933855374395E-4 18-19 6.184669276871975E-5 20-21 2.061556425623992E-5 22-23 2.061556425623992E-5 24-25 0.0 26-27 8.246225702495968E-5 28-29 3.710801566123185E-4 30-31 2.267712068186391E-4 32-33 1.236933855374395E-4 34-35 8.040070059933569E-4 36-37 6.184669276871975E-5 38-39 2.061556425623992E-4 40-41 6.184669276871976E-4 42-43 8.864692630183165E-4 44-45 0.0015873984477304736 46-47 6.184669276871975E-5 48-49 0.0 50-51 0.0 52-53 0.0011338560340931954 54-55 0.0030923346384359876 56-57 8.246225702495968E-5 58-59 6.596980561996774E-4 60-61 7.83391441737117E-4 62-63 7.42160313224637E-4 64-65 0.0014430894979367943 66-67 0.0027212544818236693 68-69 0.003298490280998387 70-71 0.0020409408613677516 72-73 0.0025357144035175103 74-75 0.0019584786043427924 76-77 0.0013400116766555947 78-79 0.0023914054537238307 80-81 0.0010307782128119959 82-83 5.153891064059979E-4 84-85 6.803136204559173E-4 86-87 0.002247096503930151 88-89 0.005978513634309577 90-91 5.978513634309577E-4 92-93 0.001298780548143115 94-95 0.0011544715983494355 96-97 3.710801566123185E-4 98-99 7.83391441737117E-4 100-101 0.0014430894979367943 102-103 0.0013400116766555947 104-105 7.215447489683971E-4 106-107 0.00766898990332125 108-109 0.0020615564256239918 110-111 0.0014018583694243145 112-113 0.002226480939673911 114-115 0.0026800233533111895 116-117 0.0018347852188053528 118-119 0.04863211608046997 120-121 0.01259610976056259 122-123 0.004494193007860302 124-125 0.0027212544818236693 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2425352.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.11525237681452 #Duplication Level Percentage of deduplicated Percentage of total 1 78.78956369324875 45.788753786922534 2 11.284889962988103 13.116484564872696 3 4.057111125832617 7.07340110995634 4 1.9507678814030296 4.534774710252833 5 1.0466428118166682 3.0412955578552223 6 0.6579783051309961 2.2943145156693907 7 0.43050344530375323 1.7513171461041046 8 0.29947220274428266 1.3923122113859643 9 0.2296278603067282 1.2010392949026079 >10 1.1245220764480668 11.569631878523884 >50 0.08427690829430909 3.397751249263841 >100 0.04371364889305443 4.458832463167647 >500 9.300775895325453E-4 0.38009151112298467 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.123112851247984E-5 2 0.0 0.0 0.0 0.0 4.123112851247984E-5 3 0.0 0.0 0.0 0.0 4.123112851247984E-5 4 0.0 0.0 0.0 0.0 4.123112851247984E-5 5 0.0 0.0 0.0 0.0 8.246225702495968E-5 6 0.0 0.0 0.0 0.0 1.236933855374395E-4 7 0.0 0.0 0.0 0.0 1.236933855374395E-4 8 0.0 0.0 0.0 0.0 1.236933855374395E-4 9 0.0 0.0 0.0 0.0 1.236933855374395E-4 10-11 0.0 0.0 0.0 0.0 1.236933855374395E-4 12-13 0.0 0.0 0.0 0.0 4.741579778935181E-4 14-15 0.0 0.0 0.0 4.123112851247984E-5 6.596980561996774E-4 16-17 0.0 0.0 0.0 4.123112851247984E-5 8.040070059933569E-4 18-19 0.0 0.0 0.0 4.123112851247984E-5 8.246225702495968E-4 20-21 0.0 0.0 0.0 6.184669276871975E-5 9.070848272745564E-4 22-23 0.0 0.0 0.0 1.030778212811996E-4 9.483159557870362E-4 24-25 0.0 0.0 0.0 4.329268493810383E-4 9.483159557870362E-4 26-27 0.0 0.0 0.0 0.0011132404698369555 9.689315200432762E-4 28-29 0.0 0.0 0.0 0.004308652929554143 9.89547084299516E-4 30-31 0.0 4.123112851247984E-5 0.0 0.017110918332679134 9.89547084299516E-4 32-33 0.0 4.123112851247984E-5 0.0 0.04461208105050318 9.89547084299516E-4 34-35 0.0 4.123112851247984E-5 0.0 0.08299826169562191 0.0011544715983494355 36-37 0.0 4.123112851247984E-5 0.0 0.1500194610926579 0.0011544715983494355 38-39 0.0 4.123112851247984E-5 0.0 0.252107735289558 0.0011750871626056755 40-41 0.0 4.123112851247984E-5 0.0 0.35945297837179924 0.0011957027268619154 42-43 0.0 4.123112851247984E-5 0.0 0.4667982214540405 0.001257549419630635 44-45 0.0 4.123112851247984E-5 0.0 0.6019538607179494 0.0013400116766555947 46-47 0.0 4.123112851247984E-5 0.0 0.7681977708802681 0.0014018583694243145 48-49 0.0 4.123112851247984E-5 0.0 0.941162354990121 0.001484320626449274 50-51 0.0 4.123112851247984E-5 0.0 1.1337323407076583 0.001566782883474234 52-53 0.0 4.123112851247984E-5 0.0 1.312098202652646 0.0016492451404991936 54-55 0.0 4.123112851247984E-5 0.0 1.496236422589381 0.0016904762690116734 56-57 0.0 4.123112851247984E-5 0.0 1.7170291157737103 0.0017317073975241532 58-59 0.0 4.123112851247984E-5 0.0 1.9529330175578636 0.0018347852188053528 60-61 0.0 4.123112851247984E-5 0.0 2.218234713971415 0.0019172474758303124 62-63 0.0 4.123112851247984E-5 0.0 2.471868001015935 0.002185249811161431 64-65 0.0 4.123112851247984E-5 0.0 2.731459186130508 0.002247096503930151 66-67 0.0 4.123112851247984E-5 0.0 3.040775112231132 0.0023295587609551112 68-69 0.0 4.123112851247984E-5 0.0 3.3815091582582655 0.002350174325211351 70-71 0.0 4.123112851247984E-5 0.0 3.7445904759391624 0.002412021017980071 72-73 0.0 4.123112851247984E-5 0.0 4.126473188221751 0.00257694553202999 74-75 0.0 4.123112851247984E-5 0.0 4.4955536351012135 0.0027212544818236693 76-77 0.0 4.123112851247984E-5 0.0 4.907308299991094 0.0027212544818236693 78-79 0.0 4.123112851247984E-5 0.0 5.362912270053997 0.002824332303104869 80-81 0.0 4.123112851247984E-5 0.0 5.8479346503105525 0.0028861789958735885 82-83 0.0 4.123112851247984E-5 0.0 6.365509006527713 0.003071719074179748 84-85 0.0 4.123112851247984E-5 0.0 6.88506245691347 0.0031129502026922278 86-87 0.0 8.246225702495968E-5 0.0 7.421623747810627 0.0032160280239734275 88-89 0.0 8.246225702495968E-5 0.0 7.971378999831777 0.003298490280998387 90-91 0.0 8.246225702495968E-5 0.0 8.583537564856567 0.003298490280998387 92-93 0.0 8.246225702495968E-5 0.0 9.191243168002005 0.0034015681022795865 94-95 0.0 8.246225702495968E-5 0.0 9.80577252291626 0.0034221836665358266 96-97 0.0 8.246225702495968E-5 0.0 10.457059428899392 0.0038138793874043847 98-99 0.0 8.246225702495968E-5 0.0 11.123436927918092 0.003958188337198064 100-101 0.0 8.246225702495968E-5 0.0 11.848362629424514 0.003958188337198064 102-103 0.0 8.246225702495968E-5 0.0 12.597202385468172 0.004102497286991744 104-105 0.0 8.246225702495968E-5 0.0 13.337589760166772 0.004205575108272943 106-107 0.0 8.246225702495968E-5 0.0 14.082182709973646 0.004205575108272943 108-109 4.123112851247984E-5 8.246225702495968E-5 0.0 14.874954233447351 0.004205575108272943 110-111 4.123112851247984E-5 8.246225702495968E-5 0.0 15.698937721205004 0.004246806236785423 112-113 4.123112851247984E-5 8.246225702495968E-5 0.0 16.54363985104018 0.004246806236785423 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1775 0.0 59.70402 1 GTATCAA 2815 0.0 46.31777 1 TAAGGTA 1045 0.0 43.85438 4 ATCAACG 3195 0.0 40.050335 3 TCAACGC 3185 0.0 39.98922 4 CAACGCA 3210 0.0 39.67696 5 GTACATG 8060 0.0 35.382 1 TATCAAC 3695 0.0 35.2751 2 AACGCAG 3630 0.0 35.083344 6 TAAGGTG 2125 0.0 34.729008 5 AGGTAAG 1875 0.0 34.599022 2 TACATGG 8055 0.0 34.431736 2 TGTAAGG 1280 0.0 33.9431 2 GTGTAAG 1435 0.0 33.190975 1 ACATGGG 8450 0.0 31.765697 3 GTAAGGT 2390 0.0 30.380894 4 ACGCAGA 4235 0.0 30.064617 7 GGTAAGG 2280 0.0 30.01937 3 CGCAGAG 4430 0.0 28.739452 8 CATGGGA 3890 0.0 27.386847 4 >>END_MODULE