FastQCFastQC Report
Thu 26 May 2016
SRR1364386_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364386_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2425352
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28650.11812718318825474No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26070.10748955203203493No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28350.074.374111
GTATCAA52450.055.644491
TCAACGC69750.041.4066934
ATCAACG69850.041.262163
CAACGCA70650.040.7106445
TAAGGTA9250.040.557564
TATCAAC73550.039.2673952
AACGCAG75150.038.3268136
TAAGGTG20350.033.944285
GTGGTAT11550.032.4885371
ACGCAGA88900.032.459127
GTACATG80400.032.225881
TACATGG80150.031.873282
CGCAGAG90500.031.8195198
AGGTAAG20100.031.107582
ACATGGG81900.030.3924263
GTAAGGT22150.030.1104554
GTGTAAG12900.028.626851
TGTAAGG12350.028.4484142
AAGGTAA22400.027.1221281
Produced by FastQC (version 0.11.5)