##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364386_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2425352 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.32476852844453 33.0 31.0 34.0 30.0 34.0 2 32.345391514303905 34.0 31.0 34.0 30.0 34.0 3 32.47088793709119 34.0 31.0 34.0 30.0 34.0 4 36.06440632122678 37.0 35.0 37.0 35.0 37.0 5 35.98847919807104 37.0 35.0 37.0 35.0 37.0 6 36.03779451395096 37.0 35.0 37.0 35.0 37.0 7 35.993407967173425 37.0 35.0 37.0 35.0 37.0 8 35.97648465047548 37.0 35.0 37.0 35.0 37.0 9 37.73903787986239 39.0 38.0 39.0 35.0 39.0 10-11 37.707686966675354 39.0 37.5 39.0 35.0 39.0 12-13 37.683295043358655 39.0 37.5 39.0 35.0 39.0 14-15 39.10853228727211 41.0 38.5 41.0 36.0 41.0 16-17 39.11319800177459 41.0 38.5 41.0 36.0 41.0 18-19 39.156518105413156 41.0 39.0 41.0 36.0 41.0 20-21 39.15529663323097 41.0 39.0 41.0 35.5 41.0 22-23 39.12768352799924 41.0 39.0 41.0 35.0 41.0 24-25 39.086872338530654 41.0 39.0 41.0 35.0 41.0 26-27 38.999369988356335 41.0 39.0 41.0 35.0 41.0 28-29 38.91804694741217 41.0 38.0 41.0 35.0 41.0 30-31 38.80233796991117 40.0 38.0 41.0 35.0 41.0 32-33 38.691156170320845 40.0 38.0 41.0 34.5 41.0 34-35 38.596265820384005 40.0 38.0 41.0 34.0 41.0 36-37 38.47649083514476 40.0 38.0 41.0 34.0 41.0 38-39 38.38368080179701 40.0 38.0 41.0 34.0 41.0 40-41 38.28132143293014 40.0 38.0 41.0 33.5 41.0 42-43 38.12827004080232 40.0 38.0 41.0 33.0 41.0 44-45 37.98728823692396 40.0 38.0 41.0 33.0 41.0 46-47 37.797339932512884 40.0 37.0 41.0 33.0 41.0 48-49 37.59229794273161 40.0 37.0 41.0 33.0 41.0 50-51 37.4216977576863 40.0 37.0 41.0 32.0 41.0 52-53 37.23864041178352 40.0 36.0 41.0 32.0 41.0 54-55 37.037334374556764 40.0 36.0 41.0 31.0 41.0 56-57 36.81978677734201 39.0 35.5 41.0 31.0 41.0 58-59 36.57957525340652 39.0 35.0 41.0 31.0 41.0 60-61 36.310215177013475 39.0 35.0 41.0 31.0 41.0 62-63 36.049403962806224 38.0 35.0 40.5 30.5 41.0 64-65 35.72344715323796 37.0 35.0 40.0 30.0 41.0 66-67 35.387050003463415 37.0 35.0 40.0 30.0 41.0 68-69 35.0272898531842 36.0 34.0 39.0 29.0 41.0 70-71 34.732664578172574 36.0 34.0 39.0 29.0 41.0 72-73 34.54729354749331 35.5 34.0 39.0 29.0 40.5 74-75 34.22885502805366 35.0 34.0 37.0 29.0 39.5 76-77 33.90859800969096 35.0 34.0 37.0 29.0 39.0 78-79 33.59446814318086 35.0 34.0 36.5 29.0 39.0 80-81 33.33339552361884 35.0 34.0 36.0 29.0 38.0 82-83 33.07009869082921 35.0 34.0 36.0 29.0 37.0 84-85 32.8616854378251 35.0 34.0 35.0 29.0 37.0 86-87 32.70490097932176 35.0 34.0 35.0 29.0 36.0 88-89 32.55473164307696 35.0 33.5 35.0 28.5 36.0 90-91 32.42601651224234 35.0 33.0 35.0 27.5 36.0 92-93 32.31368828112373 35.0 33.0 35.0 27.0 36.0 94-95 32.23291794345728 35.0 33.0 35.0 27.0 35.0 96-97 32.15631524826087 35.0 33.0 35.0 27.0 35.0 98-99 32.07992509953195 35.0 33.0 35.0 27.0 35.0 100-101 32.02461292216553 35.0 33.0 35.0 27.0 35.0 102-103 31.020370651352877 34.0 31.5 35.0 24.5 35.0 104-105 31.759152279751557 35.0 33.0 35.0 25.0 35.0 106-107 32.06951753807282 35.0 33.0 35.0 27.0 35.0 108-109 32.102820539039286 35.0 33.0 35.0 27.0 35.0 110-111 32.05328628586696 35.0 33.0 35.0 27.0 35.0 112-113 31.971692562564115 35.0 33.0 35.0 27.0 35.0 114-115 31.881978780812023 35.0 33.0 35.0 27.0 35.0 116-117 31.818076716286956 35.0 33.0 35.0 27.0 35.0 118-119 31.6993411677975 35.0 33.0 35.0 25.0 35.0 120-121 31.55114144256174 35.0 33.0 35.0 25.0 35.0 122-123 31.40153367428728 35.0 33.0 35.0 25.0 35.0 124-125 30.68462701496525 34.5 32.0 35.0 21.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10626144866184717 1101 2 0.13447372621424591 1101 3 0.19147240576351265 1101 4 0.11306867256524811 1101 5 0.19757594190492256 1101 6 0.20660487051910081 1101 7 0.2747664886533272 1101 8 0.25755964601572146 1101 9 0.23391821924687406 1101 10-11 0.30249655953326027 1101 12-13 0.205934716252969 1101 14-15 0.33820303148782216 1101 16-17 0.3624889697895668 1101 18-19 0.3449473868239039 1101 20-21 0.31554642857263815 1101 22-23 0.3107383503984096 1101 24-25 0.3409101747894354 1101 26-27 0.24480556167544165 1101 28-29 0.2228389719940438 1101 30-31 0.2357256091721851 1101 32-33 0.2211227616040219 1101 34-35 0.18222925539536305 1101 36-37 0.2584987251501687 1101 38-39 0.2519690453067156 1101 40-41 0.25684669149703154 1101 42-43 0.20689438154138173 1101 44-45 0.2604116361438287 1101 46-47 0.254808500885666 1101 48-49 0.1320599857556246 1101 50-51 0.12404055868657338 1101 52-53 0.19274610958759553 1101 54-55 0.18410675729694503 1101 56-57 0.1631383964799582 1101 58-59 0.09237278614871514 1101 60-61 0.1227363589596493 1101 62-63 -0.010109467112158654 1101 64-65 -0.032107300996365495 1101 66-67 0.022137952472171207 1101 68-69 0.0633913886740558 1101 70-71 0.04312830637597642 1101 72-73 0.09937242336028618 1101 74-75 0.1057103735933751 1101 76-77 0.053745012887887356 1101 78-79 -0.16477685974505363 1101 80-81 -0.16908964097498114 1101 82-83 -0.13000340284262535 1101 84-85 -0.3073243177123146 1101 86-87 -0.1896744447189036 1101 88-89 -0.27405683415913273 1101 90-91 -0.7251168150793248 1101 92-93 -0.6254508683731572 1101 94-95 -0.4252912653663401 1101 96-97 -0.29173002376721513 1101 98-99 -0.15206956026107576 1101 100-101 -0.23227787335336814 1101 102-103 -0.1903632488461433 1101 104-105 -0.20098765638566718 1101 106-107 -0.17852427750415956 1101 108-109 -0.05122276853449392 1101 110-111 -0.11979042110205285 1101 112-113 -0.05068817648148283 1101 114-115 -0.1850424774859114 1101 116-117 -0.14632077422461265 1101 118-119 -0.1759651678242662 1101 120-121 -0.07481208552474428 1101 122-123 0.010258258293671219 1101 124-125 -0.03475821989408345 1102 1 0.0721909244934551 1102 2 0.08529521560105735 1102 3 0.05227602385735253 1102 4 0.12879675915672095 1102 5 0.03293463224262183 1102 6 0.02667551270546653 1102 7 0.11020484112243167 1102 8 0.12860123892114927 1102 9 0.22076241866353286 1102 10-11 0.17319868217079204 1102 12-13 0.1529693039487512 1102 14-15 0.24269487981786853 1102 16-17 0.23702735092018656 1102 18-19 0.19315321859771473 1102 20-21 0.21098292647360495 1102 22-23 0.22917903793739924 1102 24-25 0.2365521925595715 1102 26-27 0.2376195737807123 1102 28-29 0.24720449894428498 1102 30-31 0.3233203607443542 1102 32-33 0.26064403012862414 1102 34-35 0.25209176445745385 1102 36-37 0.3445076523667936 1102 38-39 0.34140744835494985 1102 40-41 0.30490432641686027 1102 42-43 0.2776610768695562 1102 44-45 0.31813721906831915 1102 46-47 0.34912673634838143 1102 48-49 0.3476924356401341 1102 50-51 0.3233604379710471 1102 52-53 0.3315480164208324 1102 54-55 0.40294373232772074 1102 56-57 0.3868954986329385 1102 58-59 0.43152575430072204 1102 60-61 0.3466674533234553 1102 62-63 0.344963198239455 1102 64-65 0.32574620498039764 1102 66-67 0.2915740373102338 1102 68-69 0.21943921967232427 1102 70-71 0.31737286490446337 1102 72-73 0.2951600247292916 1102 74-75 0.30863587053334385 1102 76-77 0.31314362701968435 1102 78-79 0.3488305281800166 1102 80-81 0.3125995334049989 1102 82-83 0.20810552774594981 1102 84-85 0.20600939496449655 1102 86-87 0.26714126178725905 1102 88-89 0.2567382276317076 1102 90-91 0.16897023160521485 1102 92-93 0.2564790205213541 1102 94-95 0.20659790732767647 1102 96-97 0.2624220171007252 1102 98-99 0.304161535788694 1102 100-101 0.2593544838027384 1102 102-103 0.19150383762731238 1102 104-105 0.15651672713902443 1102 106-107 0.14235015137315088 1102 108-109 0.19428340211950257 1102 110-111 0.23005771505697226 1102 112-113 0.061583795936034136 1102 114-115 0.08403011199700572 1102 116-117 0.013483906030980819 1102 118-119 -0.046237283549317 1102 120-121 0.020184289637530384 1102 122-123 0.01510209522654904 1102 124-125 -0.17712764143741921 1103 1 0.08675881765802984 1103 2 0.16491645775098362 1103 3 0.20708854819076095 1103 4 0.1256618504480045 1103 5 -0.02288486202225215 1103 6 0.12991657302233506 1103 7 0.08728947841510859 1103 8 0.10940286172440494 1103 9 0.2502723036071117 1103 10-11 0.20979620817236366 1103 12-13 0.20427184618925054 1103 14-15 0.28422752588117817 1103 16-17 0.29182247684200036 1103 18-19 0.2649843301522523 1103 20-21 0.18088232286176975 1103 22-23 0.28055321346853646 1103 24-25 0.23429256975943247 1103 26-27 0.267606235817631 1103 28-29 0.27710687206633366 1103 30-31 0.2827589212109203 1103 32-33 0.2698656797487047 1103 34-35 0.23358350787085413 1103 36-37 0.2564850825968321 1103 38-39 0.27809852767983045 1103 40-41 0.23657648824567445 1103 42-43 0.2051381654796458 1103 44-45 0.24674979158319843 1103 46-47 0.28004382022060526 1103 48-49 0.12815941752604232 1103 50-51 -0.023646007223014465 1103 52-53 0.13480405051189592 1103 54-55 0.14255459113182667 1103 56-57 0.1361585030650332 1103 58-59 0.04173445250716412 1103 60-61 0.007052466082690501 1103 62-63 0.060955795231706134 1103 64-65 0.12847160390082735 1103 66-67 0.026049784424152733 1103 68-69 0.05632239417142415 1103 70-71 0.10167179779369917 1103 72-73 0.015201315648837976 1103 74-75 0.0892841677866798 1103 76-77 0.13584594928988025 1103 78-79 0.13387393123723967 1103 80-81 0.08234572711337762 1103 82-83 0.1544006285613051 1103 84-85 0.09616050147971578 1103 86-87 0.04340071961375713 1103 88-89 0.07260225331123848 1103 90-91 0.04191132741517123 1103 92-93 0.0664877107166717 1103 94-95 0.026990352911155924 1103 96-97 -0.07716081912051465 1103 98-99 0.011356712791730672 1103 100-101 -0.02430601584786274 1103 102-103 0.0159496052466217 1103 104-105 -0.007590677416885683 1103 106-107 -0.054761605816452175 1103 108-109 -0.004665222681047965 1103 110-111 -0.009576284289856574 1103 112-113 0.034883541488358816 1103 114-115 0.07524579815587273 1103 116-117 -0.0026319051359671164 1103 118-119 -0.10185912071836967 1103 120-121 -0.11509552785280164 1103 122-123 -0.14146530137884028 1103 124-125 -0.2093296520679786 1104 1 0.06943940332995169 1104 2 0.0796875686319467 1104 3 0.10919434316232213 1104 4 0.09760321115132342 1104 5 0.12870046969769078 1104 6 0.1473968295045296 1104 7 0.15955062249035024 1104 8 0.13952942475290087 1104 9 0.21903492189123597 1104 10-11 0.1718783053508517 1104 12-13 0.18102241188167056 1104 14-15 0.2177142970032051 1104 16-17 0.19097723206695605 1104 18-19 0.2249382799701536 1104 20-21 0.13855809883707337 1104 22-23 0.1782542030697556 1104 24-25 0.202819578459156 1104 26-27 0.10667280398521228 1104 28-29 0.23493017544790007 1104 30-31 0.2614345946012193 1104 32-33 0.16287262668766544 1104 34-35 0.22037617930254072 1104 36-37 0.2706398476542162 1104 38-39 0.2245071591761132 1104 40-41 0.10912104389853994 1104 42-43 0.15636685860560107 1104 44-45 0.16140799340233514 1104 46-47 0.17543754837622316 1104 48-49 0.13251600302491795 1104 50-51 0.1046871453028686 1104 52-53 0.12873572102849806 1104 54-55 0.112221301279682 1104 56-57 0.0928108870358102 1104 58-59 0.06685774297547908 1104 60-61 0.11377791319602437 1104 62-63 0.09264788582902383 1104 64-65 0.07275778265177024 1104 66-67 -0.010402185142503129 1104 68-69 0.04635280259742558 1104 70-71 0.09821397825940892 1104 72-73 0.18560083674398697 1104 74-75 0.18752095103536703 1104 76-77 0.12915178568874808 1104 78-79 0.1495654230396184 1104 80-81 0.15582367526894103 1104 82-83 0.08591267918651369 1104 84-85 0.10396986739291947 1104 86-87 0.17525101399668586 1104 88-89 0.10617319822079452 1104 90-91 0.08112846284836905 1104 92-93 0.1616045822474632 1104 94-95 0.11010842432605017 1104 96-97 0.08077638475949556 1104 98-99 0.12079066639839908 1104 100-101 0.072084646884079 1104 102-103 0.07645980835838984 1104 104-105 0.030060643668299747 1104 106-107 0.044362595134799676 1104 108-109 0.06851279541586308 1104 110-111 0.09367524268640182 1104 112-113 0.021888388548202187 1104 114-115 -0.07652790651991026 1104 116-117 -0.04605195339352974 1104 118-119 -0.06453943927596484 1104 120-121 0.046154590136346485 1104 122-123 -0.06404557333071992 1104 124-125 -0.2691524454742229 1105 1 0.09038979680374837 1105 2 0.13071051199801786 1105 3 0.14190596176584336 1105 4 0.059510724578998975 1105 5 0.019058528324272572 1105 6 0.06125173477747836 1105 7 0.09878977161590541 1105 8 0.1442202595444826 1105 9 0.12251151323481224 1105 10-11 0.10295638183042399 1105 12-13 0.10265386146342337 1105 14-15 0.14371084652122335 1105 16-17 0.1627636309219369 1105 18-19 0.20489112506892582 1105 20-21 0.20336827175312777 1105 22-23 0.09976601107679528 1105 24-25 0.1562464482183259 1105 26-27 0.13913123130382132 1105 28-29 0.16809336592361035 1105 30-31 0.13373926115998813 1105 32-33 0.15196295876336308 1105 34-35 0.06017626882034932 1105 36-37 0.01828810837864836 1105 38-39 0.04689284062418153 1105 40-41 0.06634325417942932 1105 42-43 0.011210809325284288 1105 44-45 0.05183179957855799 1105 46-47 0.025002255344453772 1105 48-49 0.03455025749827456 1105 50-51 -0.05968536387650403 1105 52-53 -0.11349310826051351 1105 54-55 -0.1775309948270518 1105 56-57 -3.342671168979905E-4 1105 58-59 -0.17522141580991502 1105 60-61 -0.2253277178287263 1105 62-63 -0.13862623214231462 1105 64-65 -0.19854189467948657 1105 66-67 -0.17969501334922455 1105 68-69 -0.2996253439285752 1105 70-71 -0.1999255557421833 1105 72-73 -0.15872635087929154 1105 74-75 -0.13447640894764135 1105 76-77 -0.051483601159723946 1105 78-79 -0.043644814865366754 1105 80-81 -0.06296473015067505 1105 82-83 -0.05085170688309404 1105 84-85 -0.053767828964666364 1105 86-87 -0.052640266108099354 1105 88-89 0.024032698235963323 1105 90-91 0.0035899752443100397 1105 92-93 -0.02586286007171168 1105 94-95 -0.06540070826147115 1105 96-97 -0.04749661044598952 1105 98-99 -0.07040700108083797 1105 100-101 -0.060405832364978096 1105 102-103 0.014692040083101432 1105 104-105 -0.11899158011612698 1105 106-107 -0.08683869478154449 1105 108-109 -0.08572905947423948 1105 110-111 -0.06071217348547009 1105 112-113 -0.08185508094999605 1105 114-115 -0.1827380154585434 1105 116-117 -0.16322783693944842 1105 118-119 -0.20928282785409635 1105 120-121 -0.14843002443128483 1105 122-123 -0.20076823499383423 1105 124-125 -0.37432450175412413 1106 1 0.08575018630566689 1106 2 0.018597044963229337 1106 3 -0.005818957062608376 1106 4 0.020046471949463296 1106 5 0.06367706003812401 1106 6 -0.026963432835302115 1106 7 0.057133203934483845 1106 8 0.030097322243236135 1106 9 0.07106454885310143 1106 10-11 0.05938928356502515 1106 12-13 0.044500183762281154 1106 14-15 0.06959583654373347 1106 16-17 0.10378130490543924 1106 18-19 0.09614289808626353 1106 20-21 0.09999384390241062 1106 22-23 0.03654575600528176 1106 24-25 0.135166373136542 1106 26-27 0.10488374997555638 1106 28-29 0.09544430338279142 1106 30-31 0.06875145587450504 1106 32-33 0.13165184927214568 1106 34-35 0.1378985148346672 1106 36-37 0.20725621988359677 1106 38-39 0.11482271570049107 1106 40-41 -0.018295505877787832 1106 42-43 -0.021521596420051026 1106 44-45 -0.04131539351099889 1106 46-47 -0.06926660849693178 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9 -0.22812541074741688 2216 10-11 -0.29148262067931796 2216 12-13 -0.2760140039073349 2216 14-15 -0.42437807810467376 2216 16-17 -0.4351050775293359 2216 18-19 -0.4563576316869984 2216 20-21 -0.4674059605941707 2216 22-23 -0.3603138848426255 2216 24-25 -0.5312272039608459 2216 26-27 -0.49444395067180125 2216 28-29 -0.4759352945363915 2216 30-31 -0.49609552510150934 2216 32-33 -0.5803975871174671 2216 34-35 -0.5673264166365968 2216 36-37 -0.4283431758330991 2216 38-39 -0.4370024386173341 2216 40-41 -0.6578449593568649 2216 42-43 -0.49707922549674777 2216 44-45 -0.5192341023681308 2216 46-47 -0.6819975725475445 2216 48-49 -0.5943525154054541 2216 50-51 -0.522020135837117 2216 52-53 -0.4806860701870903 2216 54-55 -0.491008786551987 2216 56-57 -0.4932835591734914 2216 58-59 -0.37511756031696564 2216 60-61 -0.4828232142944202 2216 62-63 -0.39718335148205597 2216 64-65 -0.3215818966094801 2216 66-67 -0.2938867486331773 2216 68-69 -0.5403624760570409 2216 70-71 -0.5387449768872443 2216 72-73 -0.5422027945906436 2216 74-75 -0.5415323646930048 2216 76-77 -0.5513309547877085 2216 78-79 -0.4925562814618587 2216 80-81 -0.4581023929289145 2216 82-83 -0.3944275081490858 2216 84-85 -0.3280092542179389 2216 86-87 -0.32603582527383423 2216 88-89 -0.3229467440594789 2216 90-91 -0.32806734652131553 2216 92-93 -0.3353535036885056 2216 94-95 -0.3306863304530552 2216 96-97 -0.2878853295619912 2216 98-99 -0.37728956795736224 2216 100-101 -0.42874647116921594 2216 102-103 -0.4009270351547123 2216 104-105 -0.39347080416767355 2216 106-107 -0.33838912802365684 2216 108-109 -0.2121332195635368 2216 110-111 -0.1977004475082822 2216 112-113 -0.11178493077083473 2216 114-115 -0.10232406212518086 2216 116-117 -0.08564595568451949 2216 118-119 -0.07432307177989372 2216 120-121 -0.00230190121190077 2216 122-123 0.09435139001962867 2216 124-125 0.0844323760375012 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 327.0 3 14.0 4 34.0 5 113.0 6 314.0 7 809.0 8 1365.0 9 2391.0 10 3814.0 11 5057.0 12 6689.0 13 8964.0 14 13304.0 15 10942.0 16 6334.0 17 5710.0 18 5852.0 19 5875.0 20 6304.0 21 6451.0 22 6833.0 23 7477.0 24 9061.0 25 12029.0 26 16059.0 27 21014.0 28 27277.0 29 35099.0 30 44101.0 31 55823.0 32 71344.0 33 92349.0 34 126294.0 35 193643.0 36 402719.0 37 841798.0 38 370739.0 39 1030.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.32539287140081 18.402845368852052 12.940258471933985 25.33150328781315 2 15.981309104822722 20.38029943694771 37.753777595994315 25.884613862235256 3 18.253803983916562 24.293834461966757 30.420285385379113 27.032076168737568 4 12.76185889718276 16.607774871441343 37.15200927535467 33.47835695602123 5 12.719075498405655 38.53525691670253 35.16231627073794 13.583351314153882 6 32.714327542053105 37.32344705576702 16.61232788020999 13.349897521969886 7 28.467744063542117 32.604834267355834 21.35929135234803 17.568130316754022 8 27.22161566650944 33.703973691241515 20.317257041452127 18.757153600796915 9 27.001441440252794 14.693124956707315 19.54149335848982 38.76394024455007 10-11 24.744202078708575 25.82206624028182 28.40663953108662 21.02709214992298 12-13 25.990742787026377 23.325913104572038 27.943098568785068 22.74024553961652 14-15 22.552355286985147 24.800461953563854 25.46455112495011 27.182631634500886 16-17 22.52223594760678 28.176569833986985 26.680560182604424 22.620634035801814 18-19 22.899253386724897 26.308387401086524 28.438469962298257 22.353889249890322 20-21 22.887893704216573 25.81219871560567 28.70178702374569 22.598120556432065 22-23 22.945370403966105 25.853133895615976 28.512005680000264 22.689490020417654 24-25 22.799989444831102 26.050527923369476 28.450262065052822 22.6992205667466 26-27 22.016393544011265 26.50556931075785 28.24165221855035 23.236384926680532 28-29 22.69451919324484 26.70992648263928 27.994630043430924 22.600924280684957 30-31 22.378479294517735 26.008659375891625 28.059973249199704 23.55288808039094 32-33 22.094822563826845 26.27673272858238 28.01375965788045 23.614685049710328 34-35 23.337604198985918 26.129925711676368 27.697701051086494 22.83476903825122 36-37 23.551529839792327 25.34481592774987 27.64957416490472 23.454080067553083 38-39 22.687531541813314 25.95219168186721 27.69835058993499 23.66192618638449 40-41 23.192678003726886 25.674485264907183 27.9657220536669 23.167114677699026 42-43 23.14167180681402 25.537921918138068 27.638544838027634 23.681861437020277 44-45 22.807853378748067 25.931586809496853 28.049094749584704 23.211465062170383 46-47 23.81957340625196 26.283360106079446 26.799841837391025 23.097224650277568 48-49 23.13614392035101 26.466025506098834 26.74755287038959 23.650277703160565 50-51 23.44568953290079 26.392643212201776 27.02853029168549 23.133136963211935 52-53 23.80516312683685 25.85946287384264 27.208277396435655 23.12709660288486 54-55 22.92001325993093 26.324550003463415 27.300078504068686 23.45535823253697 56-57 23.185879640414637 26.4964547370296 26.98318881117728 23.334476811378487 58-59 22.786237037953285 25.83602366668728 27.24522233904385 24.132516956315584 60-61 22.29452561711686 26.38453155852493 27.539312866467576 23.781629957890633 62-63 22.653454014097747 26.39757033205902 27.722017257701147 23.226958396142088 64-65 22.230092786531603 26.295729444633192 27.484051799491372 23.990125969343833 66-67 22.150908404223387 26.49312347238669 27.429874096625973 23.92609402676395 68-69 23.01948810306465 25.838873251980175 27.693820875036053 23.447817769919123 70-71 22.75992217208973 25.920392553490196 27.059835875302173 24.2598493991179 72-73 22.602309481581933 26.255350002133714 26.966814473542883 24.175526042741467 74-75 22.643764698897314 26.67317981060069 26.868347357414514 23.814708133087485 76-77 22.859474772929776 26.145411530124917 27.163800208712058 23.83131348823325 78-79 22.9726035643486 26.28084500724019 27.01376954767803 23.73278188073319 80-81 23.31744075225571 26.363480996831175 26.507481099084647 23.81159715182847 82-83 23.072424126477312 26.305150757498293 26.794687946326967 23.82773716969743 84-85 22.655391877137834 26.470446351704823 26.942295386401643 23.931866384755697 86-87 23.19688028789223 26.592593569923046 26.323642918636143 23.886883223548583 88-89 22.769210407396535 26.722327315787563 26.362379563873617 24.146082712942285 90-91 22.4376755286811 26.613049696095597 26.59872187598375 24.350552899239553 92-93 22.732741474227247 25.987959685851784 27.067246321358713 24.212052518562256 94-95 22.316678156407814 25.732615306974 27.396641807044915 24.554064729573273 96-97 22.715713018151593 25.321417262319034 27.29523384646847 24.6676358730609 98-99 22.354562773504284 25.73557924926727 26.85321152550941 25.05664645171904 100-101 22.24734801381408 25.385160586999326 27.24742635295825 25.12006504622834 102-103 21.910448462738604 25.052013068618496 27.536724566166065 25.50081390247684 104-105 21.766173846624838 25.570010648046498 26.93164119072579 25.732174314602872 106-107 22.086709134764053 25.58366833652875 26.423835735048552 25.90578679365865 108-109 22.091322683432068 25.305259694422823 26.788574006148487 25.814843615996626 110-111 22.16686025487671 25.409122602155403 26.992999899804186 25.431017243163694 112-113 21.99117901236604 25.758261068908762 27.173705095177937 25.07685482354726 114-115 21.89649172573713 26.058341222222587 26.58177039868852 25.46339665335176 116-117 21.822832314649585 25.84575352361224 26.86638887881017 25.465025282928007 118-119 21.30606196543842 26.21605028878282 26.483496003879026 25.99439174189973 120-121 21.28691541234185 26.387103344600472 26.78515030045247 25.540830942605208 122-123 21.184664234289915 26.54750659392901 26.58900598143681 25.678823190344268 124-125 21.09072482513352 26.326751535499714 26.743619118143958 25.838904521222812 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 53.0 1 47.0 2 92.0 3 146.0 4 98.0 5 76.5 6 181.5 7 453.5 8 781.5 9 925.0 10 712.0 11 452.5 12 431.5 13 485.0 14 554.5 15 614.5 16 740.0 17 895.0 18 1029.0 19 1202.5 20 1448.0 21 1715.5 22 1998.0 23 2600.0 24 3537.5 25 4967.0 26 7450.0 27 11134.5 28 15274.5 29 19814.5 30 24532.5 31 31426.5 32 44688.5 33 49613.0 34 45314.0 35 46382.5 36 52980.0 37 59635.0 38 61753.0 39 64599.5 40 68940.5 41 73532.0 42 75633.0 43 75673.5 44 77131.5 45 83289.0 46 86242.5 47 86345.5 48 84876.0 49 82236.5 50 84718.0 51 85991.5 52 88095.5 53 93848.0 54 100072.0 55 95875.0 56 86503.5 57 79561.5 58 73509.0 59 64256.5 60 49520.5 61 40935.5 62 36825.0 63 27011.5 64 17208.0 65 12621.0 66 9289.5 67 7035.5 68 5439.5 69 4034.0 70 2832.5 71 1727.5 72 1234.5 73 917.0 74 613.5 75 402.5 76 270.0 77 145.5 78 67.0 79 34.5 80 19.5 81 9.0 82 7.5 83 3.5 84 3.0 85 3.0 86 0.5 87 1.0 88 1.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026387922247987097 2 0.0 3 0.0 4 0.0 5 0.0607334522988828 6 0.01822415880251609 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.680023353311189E-4 22-23 0.0 24-25 0.0 26-27 2.886178995873589E-4 28-29 2.680023353311189E-4 30-31 1.6492451404991935E-4 32-33 4.123112851247984E-5 34-35 1.8554007830615926E-4 36-37 0.0 38-39 0.0 40-41 1.030778212811996E-4 42-43 0.0 44-45 4.123112851247984E-5 46-47 0.0 48-49 4.329268493810383E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 1.236933855374395E-4 58-59 8.246225702495968E-5 60-61 4.123112851247984E-5 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.061556425623992E-5 70-71 4.123112851247984E-5 72-73 1.030778212811996E-4 74-75 0.0 76-77 4.123112851247984E-5 78-79 0.0 80-81 2.680023353311189E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 2.061556425623992E-5 92-93 0.0 94-95 0.0 96-97 0.0 98-99 6.184669276871975E-5 100-101 0.0 102-103 0.0 104-105 0.001010162648555756 106-107 8.246225702495968E-5 108-109 0.0016492451404991936 110-111 0.004164343979760464 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 4.123112851247984E-4 122-123 6.184669276871976E-4 124-125 6.184669276871975E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2425352.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.955606872670714 #Duplication Level Percentage of deduplicated Percentage of total 1 76.98296099762932 42.306453404798596 2 12.125981054132048 13.327812955126678 3 4.3924237832025055 7.2416494394353785 4 2.0939786995619523 4.603034808514916 5 1.2361029158928627 3.3965392949985054 6 0.7071156429509025 2.3315981572515354 7 0.49162850621398024 1.8912420040425693 8 0.33975342434432554 1.4937084497528341 9 0.24952865200327562 1.2341698652663524 >10 1.2301531294201875 12.120208268239876 >50 0.09699341138033939 3.6843025308937745 >100 0.051033419228624796 4.942172518994627 >500 0.0015642426930745762 0.5476975392502423 >1k 7.821213465372881E-4 0.8794107634341738 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2865 0.11812718318825474 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2607 0.10748955203203493 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.123112851247984E-5 2 0.0 0.0 0.0 0.0 4.123112851247984E-5 3 0.0 0.0 0.0 0.0 4.123112851247984E-5 4 0.0 0.0 0.0 0.0 4.123112851247984E-5 5 0.0 0.0 0.0 0.0 1.6492451404991935E-4 6 0.0 0.0 0.0 0.0 3.298490280998387E-4 7 0.0 0.0 0.0 0.0 3.298490280998387E-4 8 0.0 0.0 0.0 0.0 3.298490280998387E-4 9 0.0 0.0 0.0 0.0 3.298490280998387E-4 10-11 0.0 0.0 0.0 0.0 3.504645923560786E-4 12-13 0.0 0.0 0.0 0.0 5.360046706622378E-4 14-15 0.0 0.0 0.0 4.123112851247984E-5 5.978513634309577E-4 16-17 0.0 0.0 0.0 4.123112851247984E-5 7.009291847121572E-4 18-19 0.0 0.0 0.0 4.123112851247984E-5 8.452381345058367E-4 20-21 0.0 0.0 0.0 6.184669276871975E-5 0.0010926249055807158 22-23 0.0 0.0 0.0 1.030778212811996E-4 0.001278164983886875 24-25 0.0 0.0 0.0 4.741579778935181E-4 0.0013193961123993548 26-27 0.0 0.0 0.0 0.0011132404698369555 0.0014637050621930342 28-29 0.0 0.0 0.0 0.004349884058066623 0.001525551754961754 30-31 0.0 0.0 0.0 0.017337689539497773 0.0015461673192179938 32-33 0.0 0.0 0.0 0.04506562346414046 0.0015873984477304739 34-35 0.0 0.0 0.0 0.0833074951594655 0.0016492451404991936 36-37 0.0 0.0 0.0 0.15018438560670783 0.0016492451404991936 38-39 0.0 0.0 0.0 0.25243758431765784 0.0017110918332679133 40-41 0.0 0.0 0.0 0.3593705161147743 0.0017317073975241532 42-43 0.0 0.0 0.0 0.46632406347614697 0.0017317073975241532 44-45 0.0 0.0 0.0 0.601314778226006 0.0018141696545491129 46-47 0.0 0.0 0.0 0.7683214642658056 0.0019172474758303124 48-49 0.0 0.0 0.0 0.9415952818395021 0.002164634246905191 50-51 0.0 0.0 0.0 1.134577578842164 0.0022677120681863913 52-53 0.0 0.0 0.0 1.3131495964297142 0.002350174325211351 54-55 0.0 0.0 0.0 1.49805059224393 0.0023914054537238307 56-57 0.0 0.0 0.0 1.7196266768699966 0.0024326365822363106 58-59 0.0 0.0 0.0 1.9558191965537373 0.0024944832750050305 60-61 0.0 0.0 0.0 2.2218630532805137 0.00251509883926127 62-63 4.123112851247984E-5 0.0 0.0 2.476712658616151 0.002803716738848629 64-65 4.123112851247984E-5 0.0 0.0 2.736695539451593 0.002824332303104869 66-67 4.123112851247984E-5 0.0 0.0 3.0465887013513915 0.0028861789958735885 68-69 4.123112851247984E-5 0.0 0.0 3.388807068004974 0.0029274101243860684 70-71 4.123112851247984E-5 0.0 0.0 3.7531459351055023 0.0029892568171547883 72-73 4.123112851247984E-5 0.0 0.0 4.137440668406071 0.003133565766948468 74-75 4.123112851247984E-5 0.0 0.0 4.507304506727271 0.003339721409510867 76-77 4.123112851247984E-5 0.0 0.0 4.918976709360126 0.0034634147950483064 78-79 4.123112851247984E-5 0.0 0.0 5.377384396161877 0.0034634147950483064 80-81 4.123112851247984E-5 0.0 0.0 5.865870191213482 0.0035664926163295058 82-83 4.123112851247984E-5 0.0 0.0 6.387815047052964 0.0037108015661231853 84-85 4.123112851247984E-5 0.0 0.0 6.9108112966695145 0.003752032694635665 86-87 4.123112851247984E-5 0.0 0.0 7.449970148662957 0.0038963416444293443 88-89 4.123112851247984E-5 0.0 0.0 8.002549732987212 0.004040650594223024 90-91 4.123112851247984E-5 0.0 0.0 8.616687392180598 0.004040650594223024 92-93 4.123112851247984E-5 0.0 0.0 9.22696994085807 0.004329268493810383 94-95 4.123112851247984E-5 0.0 0.0 9.845148250645678 0.004370499622322862 96-97 4.123112851247984E-5 0.0 0.0 10.49824932628336 0.004865273164472621 98-99 4.123112851247984E-5 0.0 0.0 11.168605629203514 0.005153891064059979 100-101 4.123112851247984E-5 0.0 0.0 11.897757521382463 0.005153891064059979 102-103 4.123112851247984E-5 0.0 0.0 12.650720390277371 0.00525696888534118 104-105 8.246225702495968E-5 0.0 0.0 13.3953339556485 0.005318815578109899 106-107 8.246225702495968E-5 0.0 0.0 14.144091249435133 0.005318815578109899 108-109 8.246225702495968E-5 0.0 0.0 14.940965270195832 0.005421893399391099 110-111 8.246225702495968E-5 0.0 0.0 15.770721115945232 0.005483740092159819 112-113 8.246225702495968E-5 0.0 0.0 16.620535905715954 0.005524971220672298 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2835 0.0 74.37411 1 GTATCAA 5245 0.0 55.64449 1 TCAACGC 6975 0.0 41.406693 4 ATCAACG 6985 0.0 41.26216 3 CAACGCA 7065 0.0 40.710644 5 TAAGGTA 925 0.0 40.55756 4 TATCAAC 7355 0.0 39.267395 2 AACGCAG 7515 0.0 38.326813 6 TAAGGTG 2035 0.0 33.94428 5 GTGGTAT 1155 0.0 32.488537 1 ACGCAGA 8890 0.0 32.45912 7 GTACATG 8040 0.0 32.22588 1 TACATGG 8015 0.0 31.87328 2 CGCAGAG 9050 0.0 31.819519 8 AGGTAAG 2010 0.0 31.10758 2 ACATGGG 8190 0.0 30.392426 3 GTAAGGT 2215 0.0 30.110455 4 GTGTAAG 1290 0.0 28.62685 1 TGTAAGG 1235 0.0 28.448414 2 AAGGTAA 2240 0.0 27.122128 1 >>END_MODULE