FastQCFastQC Report
Thu 26 May 2016
SRR1364385_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364385_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1655579
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14250.051.3927231
GTATCAA20100.045.91421
GTACATG57100.037.2257541
ATCAACG23750.037.089593
TCAACGC23950.036.779864
CAACGCA23900.036.6077735
AACGCAG24850.034.9645466
TACATGG60100.034.7605442
ACATGGG59950.034.152553
CATGGGG38950.032.2425164
TATCAAC28050.031.8282152
ACGCAGA31000.027.8284917
CGCAGAG31600.027.1110088
CCTACGC4400.024.3382996-97
CGCGGAA4300.024.21433882-83
AATTCGC4550.024.18970378-79
CGCGATC5200.022.88215322-23
CGCTCCG5450.021.83350248-49
ATGGGGA22400.021.7881185
GCAGAGT38100.021.7050139
Produced by FastQC (version 0.11.5)