FastQCFastQC Report
Thu 26 May 2016
SRR1364385_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364385_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1655579
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27240.16453458276530447No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27220.16441377910688645No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24000.072.956771
GTATCAA42350.051.470361
ATCAACG50400.042.4170953
TCAACGC50850.042.158834
CAACGCA51250.041.7135935
AACGCAG53050.040.3836366
TATCAAC53450.040.2194862
GTACATG54800.033.7994271
ACGCAGA63450.033.6634987
CGCAGAG64500.033.2999768
ACATGGG55000.031.1822033
TACATGG57950.030.6224442
GCAGAGT73350.029.0388479
CATGGGG37200.027.0533314
GAGTACT46350.026.44319512-13
CAGAGTA74750.025.27138110-11
GTACTTT48400.025.26171914-15
CAATACA11800.023.7188074
CCAATAC11400.022.983963
ATGGGGA22750.022.7727175
Produced by FastQC (version 0.11.5)