##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364385_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1655579 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.28170688321125 33.0 31.0 34.0 30.0 34.0 2 32.306889009826776 33.0 31.0 34.0 30.0 34.0 3 32.42910486301167 34.0 31.0 34.0 30.0 34.0 4 36.03326509940027 37.0 35.0 37.0 35.0 37.0 5 35.96063310781304 37.0 35.0 37.0 35.0 37.0 6 36.01814470949438 37.0 35.0 37.0 35.0 37.0 7 35.978859963795145 37.0 35.0 37.0 35.0 37.0 8 35.98835271527363 37.0 35.0 37.0 35.0 37.0 9 37.73813692973878 39.0 38.0 39.0 35.0 39.0 10-11 37.70363389484888 39.0 37.5 39.0 35.0 39.0 12-13 37.66351167778765 39.0 37.0 39.0 35.0 39.0 14-15 39.07677102693378 41.0 38.0 41.0 36.0 41.0 16-17 39.080600200896484 41.0 38.0 41.0 36.0 41.0 18-19 39.12619089756514 41.0 38.5 41.0 36.0 41.0 20-21 39.133563544838395 41.0 39.0 41.0 35.5 41.0 22-23 39.09365635828915 41.0 39.0 41.0 35.0 41.0 24-25 39.05939946085327 41.0 39.0 41.0 35.0 41.0 26-27 38.97080175576037 41.0 39.0 41.0 35.0 41.0 28-29 38.886253087288495 40.5 38.0 41.0 35.0 41.0 30-31 38.786160914097124 40.0 38.0 41.0 35.0 41.0 32-33 38.67447430777993 40.0 38.0 41.0 34.5 41.0 34-35 38.58505936593784 40.0 38.0 41.0 34.0 41.0 36-37 38.47697391667809 40.0 38.0 41.0 34.0 41.0 38-39 38.39125737883846 40.0 38.0 41.0 34.0 41.0 40-41 38.28642698415479 40.0 38.0 41.0 34.0 41.0 42-43 38.14792528776941 40.0 38.0 41.0 33.0 41.0 44-45 37.99767785167606 40.0 38.0 41.0 33.0 41.0 46-47 37.79660982653199 40.0 37.5 41.0 33.0 41.0 48-49 37.603496722294736 40.0 37.0 41.0 33.0 41.0 50-51 37.44589385344946 40.0 37.0 41.0 32.0 41.0 52-53 37.293688190053146 40.0 37.0 41.0 32.0 41.0 54-55 37.1098220018495 40.0 36.0 41.0 31.5 41.0 56-57 36.92073437751989 39.5 36.0 41.0 31.0 41.0 58-59 36.70901237573079 39.0 35.0 41.0 31.0 41.0 60-61 36.45313542875333 39.0 35.0 41.0 31.0 41.0 62-63 36.209004825502134 38.5 35.0 41.0 31.0 41.0 64-65 35.9141819870873 38.0 35.0 40.0 30.0 41.0 66-67 35.58747030495071 37.0 35.0 40.0 30.0 41.0 68-69 35.25852617120657 37.0 35.0 39.5 29.5 41.0 70-71 35.00034066631674 36.0 34.5 39.0 29.5 41.0 72-73 34.79728934711059 36.0 34.5 39.0 30.0 41.0 74-75 34.45948456703063 35.5 34.0 38.5 29.5 40.0 76-77 34.125091584273534 35.0 34.0 37.0 29.0 39.0 78-79 33.8105792595823 35.0 34.0 37.0 29.0 39.0 80-81 33.516958417568716 35.0 34.0 36.0 29.0 39.0 82-83 33.24724522357435 35.0 34.0 36.0 29.0 37.0 84-85 33.015555283076196 35.0 34.0 36.0 29.0 37.0 86-87 32.82622756147547 35.0 34.0 35.0 29.0 36.5 88-89 32.675727041717735 35.0 34.0 35.0 29.0 36.0 90-91 32.54048160794501 35.0 34.0 35.0 29.0 36.0 92-93 32.421017662098876 35.0 33.0 35.0 27.5 36.0 94-95 32.34177469030472 35.0 33.0 35.0 27.5 35.5 96-97 32.26280262071457 35.0 33.0 35.0 27.0 35.0 98-99 32.19646782183152 35.0 33.0 35.0 27.0 35.0 100-101 32.139221082171254 35.0 33.0 35.0 27.0 35.0 102-103 31.149885629136392 34.0 31.5 35.0 25.0 35.0 104-105 31.882376195880717 35.0 33.0 35.0 26.0 35.0 106-107 32.18010406027136 35.0 33.5 35.0 27.5 35.0 108-109 32.19397050820287 35.0 34.0 35.0 28.0 35.0 110-111 32.17219444073645 35.0 34.0 35.0 27.0 35.0 112-113 32.11780500960691 35.0 34.0 35.0 27.0 35.0 114-115 32.04943013894233 35.0 34.0 35.0 27.0 35.0 116-117 32.0032873695547 35.0 34.0 35.0 27.0 35.0 118-119 31.906638402637384 35.0 34.0 35.0 27.0 35.0 120-121 31.779132255241215 35.0 33.0 35.0 26.5 35.0 122-123 31.641731080184034 35.0 33.0 35.0 25.0 35.0 124-125 30.96132319871175 34.5 32.0 35.0 22.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11957175649693141 1101 2 0.1735825956215038 1101 3 0.1675086945499018 1101 4 0.1866449637261809 1101 5 0.22298674040694522 1101 6 0.23114123160387834 1101 7 0.27232654779003695 1101 8 0.2923869002101114 1101 9 0.2712941366352979 1101 10-11 0.3432995714307907 1101 12-13 0.2986266791010195 1101 14-15 0.4038755448405169 1101 16-17 0.3396196566443521 1101 18-19 0.32392824531688547 1101 20-21 0.2772782235865492 1101 22-23 0.2778721890661089 1101 24-25 0.3495090497308624 1101 26-27 0.2913943334293805 1101 28-29 0.2284647579993475 1101 30-31 0.3356292199686308 1101 32-33 0.2538720448824563 1101 34-35 0.21751409658661203 1101 36-37 0.2778951693127567 1101 38-39 0.24147417371911928 1101 40-41 0.24134816806962078 1101 42-43 0.31658595998762706 1101 44-45 0.29047798540678116 1101 46-47 0.2885593777184212 1101 48-49 0.15566302147700384 1101 50-51 0.15649932486049067 1101 52-53 0.1724572585529245 1101 54-55 0.21780087873141696 1101 56-57 0.31996697268459684 1101 58-59 0.2009762879133632 1101 60-61 0.3531622527101348 1101 62-63 0.1657616297179061 1101 64-65 0.09367054455144341 1101 66-67 0.06427442323185062 1101 68-69 0.19493051740728617 1101 70-71 0.30703131522194127 1101 72-73 0.28625337391833483 1101 74-75 0.231259860127615 1101 76-77 0.11548113556525408 1101 78-79 -0.01740647765086578 1101 80-81 -0.05641873605648584 1101 82-83 0.0409609007156746 1101 84-85 -0.2490179447825014 1101 86-87 -0.14385498569972555 1101 88-89 -0.2379207574581983 1101 90-91 -0.6242571500914131 1101 92-93 -0.5612824908165557 1101 94-95 -0.4388699914499128 1101 96-97 -0.2503092375030249 1101 98-99 -0.09566732456269023 1101 100-101 -0.2275744003233342 1101 102-103 -0.0037107073679401026 1101 104-105 -0.08846011529738362 1101 106-107 -0.11966685219868367 1101 108-109 -0.027687841126095236 1101 110-111 -0.011794626050800616 1101 112-113 -0.05924481480256105 1101 114-115 -0.11248692348554457 1101 116-117 -0.11750508199484244 1101 118-119 -0.12142716057118719 1101 120-121 -0.07836236093618609 1101 122-123 0.007776682752798081 1101 124-125 -0.01168772014280961 1102 1 0.11530103876826558 1102 2 0.1250931266921924 1102 3 0.16304971616310837 1102 4 0.13717302663927455 1102 5 0.04737467372205373 1102 6 0.06422373368133094 1102 7 0.1957286099852098 1102 8 0.180893779133811 1102 9 0.24237389658375008 1102 10-11 0.2464347974698171 1102 12-13 0.19368206505732388 1102 14-15 0.30027290545901764 1102 16-17 0.2793040703084486 1102 18-19 0.34480651696329545 1102 20-21 0.26911413274282125 1102 22-23 0.2980673983537869 1102 24-25 0.27827132646634567 1102 26-27 0.1986663541633007 1102 28-29 0.15136076676361654 1102 30-31 0.24095057487455307 1102 32-33 0.18019818479251626 1102 34-35 0.19121990061330507 1102 36-37 0.2723041570851379 1102 38-39 0.2919955404805208 1102 40-41 0.25632504439169423 1102 42-43 0.1135682459220817 1102 44-45 0.2428581928996607 1102 46-47 0.2589582707676996 1102 48-49 0.1746710360021666 1102 50-51 0.11923112422621784 1102 52-53 0.1298584820825468 1102 54-55 0.17011467357230714 1102 56-57 0.18599838312466943 1102 58-59 0.27663731334666153 1102 60-61 0.15194893197873682 1102 62-63 0.13722285076666196 1102 64-65 0.03460856563005166 1102 66-67 0.04734770437911351 1102 68-69 -0.03306905667356119 1102 70-71 0.10602793531193555 1102 72-73 0.15807805329890812 1102 74-75 0.16763955827387633 1102 76-77 0.1658131439387418 1102 78-79 0.11158198630681682 1102 80-81 0.11092907194178991 1102 82-83 0.11515535915616937 1102 84-85 0.07637825334016668 1102 86-87 0.009753361876654765 1102 88-89 -0.025609114167686187 1102 90-91 0.06356990477641489 1102 92-93 0.07114076963849669 1102 94-95 -0.11626339914346318 1102 96-97 -0.020021994550170064 1102 98-99 0.0191859473855871 1102 100-101 0.01995900084970259 1102 102-103 0.057800032271412505 1102 104-105 -0.012797443269402464 1102 106-107 -0.024790066878402683 1102 108-109 0.010582861234354368 1102 110-111 0.034273250972724156 1102 112-113 -0.10677486789975177 1102 114-115 -0.1652070430063617 1102 116-117 -0.09714658540164578 1102 118-119 -0.11423538388455512 1102 120-121 -0.17258407600724368 1102 122-123 -0.140392413738752 1102 124-125 -0.25684755508815726 1103 1 0.09992456777253267 1103 2 0.18289331373476614 1103 3 0.20603848895601118 1103 4 0.1136034913144357 1103 5 0.04758198182376816 1103 6 0.08058866624933358 1103 7 0.1683081069554646 1103 8 0.21586370911950326 1103 9 0.2681865986251424 1103 10-11 0.30146811927454564 1103 12-13 0.25830222336627173 1103 14-15 0.3345418135346776 1103 16-17 0.3313603753956684 1103 18-19 0.37760694957032825 1103 20-21 0.2944015853295028 1103 22-23 0.33040591128779084 1103 24-25 0.34427646832764225 1103 26-27 0.44519394345957863 1103 28-29 0.4461854570034376 1103 30-31 0.43789301301428196 1103 32-33 0.4230138734147033 1103 34-35 0.40259429129033464 1103 36-37 0.48029906381976417 1103 38-39 0.4656538150464016 1103 40-41 0.440783760870211 1103 42-43 0.45302104558955847 1103 44-45 0.40030352637361943 1103 46-47 0.5157359790981317 1103 48-49 0.4059669080894466 1103 50-51 0.2902318743610266 1103 52-53 0.37753557153883577 1103 54-55 0.3783479070772273 1103 56-57 0.32353412567798756 1103 58-59 0.32779433553379533 1103 60-61 0.29117803534793296 1103 62-63 0.24032632025643608 1103 64-65 0.25780959917209145 1103 66-67 0.13152177287556555 1103 68-69 0.2184173837739607 1103 70-71 0.28143643825276143 1103 72-73 0.32152963496666587 1103 74-75 0.3115240468199758 1103 76-77 0.3366143406389739 1103 78-79 0.28718280499288085 1103 80-81 0.3310442649373613 1103 82-83 0.34898080366662043 1103 84-85 0.38005437914981854 1103 86-87 0.3465393315124743 1103 88-89 0.32796829472918887 1103 90-91 0.43310279981595556 1103 92-93 0.3937923074665406 1103 94-95 0.3743687044547528 1103 96-97 0.35989724317638405 1103 98-99 0.3527800960239418 1103 100-101 0.2873536239702972 1103 102-103 0.3640083153932778 1103 104-105 0.3577373547654652 1103 106-107 0.3419785478123458 1103 108-109 0.33311911301081665 1103 110-111 0.3126973371756705 1103 112-113 0.32315809558765096 1103 114-115 0.31753804712613487 1103 116-117 0.29247580883632196 1103 118-119 0.27500806561886293 1103 120-121 0.3775873279855766 1103 122-123 0.2838341486845124 1103 124-125 0.17927906104909752 1104 1 0.0559595080279891 1104 2 0.06565573647889522 1104 3 0.05520948195147923 1104 4 0.10446018682329594 1104 5 0.12157291713485563 1104 6 0.109448493196183 1104 7 0.11099636406300561 1104 8 0.1166721145583196 1104 9 0.18355800365192465 1104 10-11 0.13133124247190864 1104 12-13 0.1743355154964732 1104 14-15 0.14672401378189903 1104 16-17 0.19836663827164358 1104 18-19 0.22214679299842288 1104 20-21 0.2003987835165546 1104 22-23 0.13408881198824218 1104 24-25 0.1821624180598178 1104 26-27 0.1309791103322695 1104 28-29 0.2527919670982044 1104 30-31 0.26430883501675595 1104 32-33 0.1926225260723129 1104 34-35 0.18491007208967858 1104 36-37 0.21816078611065137 1104 38-39 0.17827977301921294 1104 40-41 0.08771684698475468 1104 42-43 0.1914778235046839 1104 44-45 0.15929775793521372 1104 46-47 0.1933665518216543 1104 48-49 0.18557885699756582 1104 50-51 0.07542058470301072 1104 52-53 0.12942226292737757 1104 54-55 0.2302607212511063 1104 56-57 0.19891080470559075 1104 58-59 0.19962878135167728 1104 60-61 0.2841998730075943 1104 62-63 0.1905595299803764 1104 64-65 0.19947824358906985 1104 66-67 0.1980383777375394 1104 68-69 0.1054640257187458 1104 70-71 0.2804400641283351 1104 72-73 0.33192773962262123 1104 74-75 0.3134299388677704 1104 76-77 0.27012715481284744 1104 78-79 0.29022606828088726 1104 80-81 0.19693944417098663 1104 82-83 0.2019985210131381 1104 84-85 0.33756595722974936 1104 86-87 0.28407414815853826 1104 88-89 0.22189009118572756 1104 90-91 0.1843570231369327 1104 92-93 0.2130319448789848 1104 94-95 0.26096202954746417 1104 96-97 0.19655550440423752 1104 98-99 0.23645813475489064 1104 100-101 0.2330576206740389 1104 102-103 0.1653195638481577 1104 104-105 0.15740130202545188 1104 106-107 0.22192737352309422 1104 108-109 0.274857390719923 1104 110-111 0.26467484026590427 1104 112-113 0.30336130943190653 1104 114-115 0.2525911167594259 1104 116-117 0.16962982614163735 1104 118-119 0.12068272569302962 1104 120-121 0.20049363206469195 1104 122-123 0.04152611407388207 1104 124-125 -0.23135596161262484 1105 1 0.016311458105988663 1105 2 0.057800510269842675 1105 3 0.05028904513400789 1105 4 0.06041400897618132 1105 5 -0.005038627326925393 1105 6 0.0666522373459486 1105 7 0.08516921897719953 1105 8 0.11753638912929887 1105 9 0.12814271504037578 1105 10-11 0.09882359816613473 1105 12-13 0.12941757477884153 1105 14-15 0.13336270332948175 1105 16-17 0.10994448538553314 1105 18-19 0.14543758863025147 1105 20-21 0.06404683343856021 1105 22-23 0.04757991963254682 1105 24-25 0.08714900152315863 1105 26-27 0.13135383732134898 1105 28-29 0.0031476613259826536 1105 30-31 0.056548148589293135 1105 32-33 0.0476824324685623 1105 34-35 0.11772879283850557 1105 36-37 0.10124471746791386 1105 38-39 0.07639896178675798 1105 40-41 0.024405146516734533 1105 42-43 -0.04449222329719049 1105 44-45 0.004528647170786826 1105 46-47 0.015604929356761943 1105 48-49 0.0018337711941285306 1105 50-51 -0.04587270702865709 1105 52-53 -0.12402079479493011 1105 54-55 -0.12556486377228993 1105 56-57 -0.0677796789137517 1105 58-59 -0.24510226389013212 1105 60-61 -0.21827594602516598 1105 62-63 -0.1597181611272731 1105 64-65 -0.14140069556849966 1105 66-67 -0.08566115424373777 1105 68-69 -0.19825297896144178 1105 70-71 -0.1477103258872674 1105 72-73 -0.036246363341497556 1105 74-75 -0.002770997169676548 1105 76-77 0.051305313939217 1105 78-79 0.015439796830037267 1105 80-81 -0.008990041008424043 1105 82-83 0.022759831465549496 1105 84-85 0.06088920215955085 1105 86-87 -0.02368872235627606 1105 88-89 0.050524100546098794 1105 90-91 -0.01599711102844026 1105 92-93 -0.06619863234410417 1105 94-95 -0.11258867248061932 1105 96-97 -0.027604870010733862 1105 98-99 -0.07843702905166339 1105 100-101 -0.1347174183275186 1105 102-103 -0.08113784644825373 1105 104-105 -0.15261461061104953 1105 106-107 -0.12218557499484461 1105 108-109 -0.1546362130242258 1105 110-111 -0.16303998032691425 1105 112-113 -0.13592979821239837 1105 114-115 -0.19866535749954295 1105 116-117 -0.20406346559002486 1105 118-119 -0.20531446619464688 1105 120-121 -0.1965481776076956 1105 122-123 -0.2683291121351701 1105 124-125 -0.44781524398392136 1106 1 0.12802228468795107 1106 2 0.13553823144871302 1106 3 0.15436843567381686 1106 4 0.16325052887560787 1106 5 0.1776373034527623 1106 6 0.07526177287424929 1106 7 0.16631145460626584 1106 8 0.13904233588628756 1106 9 0.20884894932596865 1106 10-11 0.1651827515262312 1106 12-13 0.19634597827313627 1106 14-15 0.1762849795766641 1106 16-17 0.2536056724768727 1106 18-19 0.18077818132840662 1106 20-21 0.24931789820468708 1106 22-23 0.13492905343694162 1106 24-25 0.23000708606786446 1106 26-27 0.1982897743161729 1106 28-29 0.1749770777621933 1106 30-31 0.2379442474916047 1106 32-33 0.3124973751668847 1106 34-35 0.27596761736130304 1106 36-37 0.2621157156881182 1106 38-39 0.22082464223450415 1106 40-41 0.27602147475135297 1106 42-43 0.26970599251877303 1106 44-45 0.294370192542857 1106 46-47 0.2292550830087734 1106 48-49 0.18722835397944948 1106 50-51 0.1925574056285626 1106 52-53 0.10825727298773558 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42-43 -0.6621725352243928 2216 44-45 -0.6294207251532669 2216 46-47 -0.7651949104263807 2216 48-49 -0.7539217238018949 2216 50-51 -0.5998964634691788 2216 52-53 -0.7043081777969107 2216 54-55 -0.5400475807646075 2216 56-57 -0.6864117781437358 2216 58-59 -0.5623261896083491 2216 60-61 -0.703010239735832 2216 62-63 -0.7077035380073227 2216 64-65 -0.6372589617406277 2216 66-67 -0.6634702659249143 2216 68-69 -0.7565661075234829 2216 70-71 -0.751941828594596 2216 72-73 -0.7461509074562969 2216 74-75 -0.8406614857991244 2216 76-77 -0.7432092305646663 2216 78-79 -0.7394582501338647 2216 80-81 -0.7104495548040788 2216 82-83 -0.6655361561306989 2216 84-85 -0.5800222217931363 2216 86-87 -0.6559316598359928 2216 88-89 -0.49807854564379994 2216 90-91 -0.46521386856105096 2216 92-93 -0.42779299533283677 2216 94-95 -0.5018538228611469 2216 96-97 -0.5152550866446148 2216 98-99 -0.5707118686615757 2216 100-101 -0.5949654746453987 2216 102-103 -0.6988356804292586 2216 104-105 -0.6522619709502884 2216 106-107 -0.5798614204639208 2216 108-109 -0.42954944221482094 2216 110-111 -0.33886099847683226 2216 112-113 -0.3256968388427772 2216 114-115 -0.39024243295017413 2216 116-117 -0.36591947348254905 2216 118-119 -0.37728529617236006 2216 120-121 -0.4220187013825232 2216 122-123 -0.40672069152912727 2216 124-125 -0.4307371710420753 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 262.0 3 16.0 4 31.0 5 105.0 6 218.0 7 511.0 8 937.0 9 1596.0 10 2537.0 11 3405.0 12 4714.0 13 6451.0 14 9286.0 15 7953.0 16 4271.0 17 3918.0 18 3948.0 19 3720.0 20 3437.0 21 3679.0 22 4004.0 23 4350.0 24 5789.0 25 7910.0 26 10613.0 27 13882.0 28 17953.0 29 22928.0 30 29175.0 31 37302.0 32 46985.0 33 61517.0 34 83984.0 35 128715.0 36 260237.0 37 571993.0 38 285829.0 39 1418.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.21420868231836 18.892105099395405 13.184489447224642 24.7091967710616 2 16.076490460437103 20.28178661362581 38.56813839750323 25.073584528433855 3 18.402262894129485 24.606859594135948 30.151505908204925 26.839371603529642 4 12.855079703233733 16.795211826194944 37.16180260803018 33.187905862541136 5 13.243436484437279 37.959751305933956 34.922876916744784 13.873935292883981 6 32.46697307341921 37.074190064011944 16.808391872005103 13.650444990563734 7 28.12967547909221 32.47498307238737 21.46360880392902 17.93173264459141 8 26.204850387689138 35.455813343851304 19.718237547105876 18.621098721353675 9 26.324929224156623 15.270428049643057 19.842846520764034 38.56179620543628 10-11 25.032088471767278 26.108388666442373 27.99996255086589 20.859560310924454 12-13 25.63565375013817 23.51624416593832 28.30780047342954 22.540301610493973 14-15 22.693420247538775 25.11785906924405 25.707441324153063 26.481279359064107 16-17 22.549029674814673 28.419423053807762 26.433592114903607 22.597955156473958 18-19 22.441091606018198 26.880354244647947 28.42757730075098 22.25097684858288 20-21 22.911363236199705 26.252626358934062 28.663485690590836 22.1725247142754 22-23 22.89824285038648 25.973360377245662 28.497039404341322 22.631357368026535 24-25 22.79550622335335 26.37217141923503 28.31614447759499 22.51617787981663 26-27 22.57222199571447 26.643291066987402 28.311475335571227 22.473011601726895 28-29 22.433156113831217 26.473809083737727 28.593587480837463 22.4994473215936 30-31 22.560493314862416 26.305777054565016 28.545753425319475 22.5879762052531 32-33 22.422779966507157 26.407884861928842 28.50407184200075 22.66526332956325 34-35 22.961079093040457 26.38792371595255 28.05387008928315 22.597127101723842 36-37 23.003855448758408 26.086795012500158 28.042243831312184 22.867105707429243 38-39 22.620152828708264 26.317741406480756 28.29602211673378 22.7660836480772 40-41 22.860707433332138 26.10296171756485 28.232552225765467 22.80377862333755 42-43 22.762761547470703 26.132005781663093 28.144473927248413 22.96075874361779 44-45 22.737849927200585 26.358371238055145 28.009367129818525 22.894411704925748 46-47 23.03559661000774 26.700084985373696 27.349163042053565 22.915155362565002 48-49 23.017740685551168 26.63304487328556 27.306920323054314 23.04229411810896 50-51 23.047676975849537 26.565841919956707 27.310022656726137 23.076458447467623 52-53 23.227463020490113 26.53162428370981 27.164454248332458 23.076458447467623 54-55 22.99391934785353 26.435796781669733 27.3252137167722 23.245070153704532 56-57 22.82933553035318 26.8247123659681 27.333326890459876 23.012625213218843 58-59 22.95990371939701 26.430384563694542 27.30059450554263 23.309117211365823 60-61 22.98745635212817 26.444524845990436 27.611397583564422 22.956621218316975 62-63 22.759681054181044 26.573633755924664 27.572468604639223 23.09421658525507 64-65 22.66886690396532 26.366183673506367 27.4608762251756 23.50407319735271 66-67 22.575244068691376 26.68271945947611 27.34414969022922 23.397886781603294 68-69 22.469161827470526 26.43886304360169 27.685376345904572 23.40659878302321 70-71 22.466128164225324 26.319493059527815 27.60744126375123 23.60693751249563 72-73 22.630260437823054 26.36433510361188 27.224669337436637 23.780735121128426 74-75 22.58998211501837 26.625609529959004 27.149383991944813 23.63502436307781 76-77 22.676688940847882 26.49166847368806 27.252157704343915 23.579484881120138 78-79 22.675480904263704 26.515950009030075 27.188222368126198 23.620346718580027 80-81 22.51974539374816 26.8220244796977 27.144238621482796 23.513991505071346 82-83 22.652679213737308 26.656686271087032 27.00855712714404 23.68207738803162 84-85 22.7143796822743 26.340573297921754 27.052227649662143 23.892819370141805 86-87 22.626193011629166 26.333596886648113 27.25176509245406 23.78844500926866 88-89 22.430128674016764 26.5705230617204 27.11564957033159 23.883698693931247 90-91 22.517620723625996 26.664115696079737 27.076992399637827 23.741271180656433 92-93 22.450574693204008 26.1488578920124 27.24995303757779 24.150614377205798 94-95 22.292472905249465 26.093409012798546 27.329351242073013 24.28476683987898 96-97 22.442541249919213 26.17552529960817 27.231772086985885 24.15016136348673 98-99 22.36686808699812 26.043413289392937 27.096672094787554 24.493046528821388 100-101 22.0724894432703 26.005494150384852 27.32796200000121 24.59405440634364 102-103 22.16403510917785 25.825444097033152 27.304473934639766 24.706046859149232 104-105 22.04448769267661 25.945375549889793 27.31506397537025 24.695072782063342 106-107 22.050140766462974 25.961280011403865 27.33992156218459 24.648657659948576 108-109 22.081245409386113 25.80722321014381 27.074746066568732 25.036785313901344 110-111 21.87519820236121 26.12338833165711 27.207800640892053 24.793612825089625 112-113 21.93564909919732 26.058828965576392 27.338200110052135 24.667321825174156 114-115 21.728138614949817 26.08540577042835 27.15944089650811 25.027014718113723 116-117 21.506258990437484 25.975844697185906 27.417848202305116 25.100048110071494 118-119 21.431716638106668 26.07782534086262 27.047063293264774 25.443394727765934 120-121 21.452378550671764 26.266831846732874 27.16305048361075 25.117739118984613 122-123 21.403815023315374 26.319130686897484 27.115024523424097 25.16202976636304 124-125 21.28374501231594 26.25342931592471 27.125481251864908 25.337344419894443 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 69.0 1 61.0 2 93.0 3 141.0 4 98.5 5 73.5 6 192.0 7 466.5 8 844.0 9 1065.5 10 876.0 11 624.5 12 621.0 13 738.5 14 872.5 15 961.5 16 1029.0 17 1138.0 18 1286.0 19 1415.5 20 1561.5 21 1837.5 22 2231.0 23 2816.5 24 3719.0 25 4929.0 26 6777.5 27 9011.5 28 10985.5 29 13480.5 30 16590.5 31 19600.0 32 22865.5 33 26153.5 34 29519.5 35 32803.0 36 37471.5 37 42332.5 38 45109.5 39 47364.0 40 48653.5 41 51061.0 42 54226.5 43 57054.0 44 59948.0 45 62408.5 46 62919.0 47 62661.0 48 63459.0 49 62850.5 50 61360.5 51 59703.0 52 58623.0 53 59169.5 54 59892.5 55 56454.5 56 50658.5 57 47061.0 58 44208.0 59 39558.0 60 33041.0 61 26462.5 62 20940.0 63 15836.0 64 12393.0 65 9720.0 66 6905.5 67 5797.0 68 4247.5 69 2431.0 70 1721.0 71 1217.5 72 858.5 73 655.5 74 579.5 75 511.5 76 297.0 77 119.0 78 52.5 79 28.0 80 17.5 81 7.5 82 4.0 83 4.5 84 3.5 85 2.5 86 1.0 87 1.0 88 1.0 89 1.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025489571926196216 2 0.0 3 0.0 4 0.0 5 0.06136825847633969 6 0.015885681081965888 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 4.5301371906746827E-4 22-23 0.0 24-25 3.0200914604497883E-5 26-27 3.9261188985847246E-4 28-29 3.020091460449788E-4 30-31 2.1140640223148518E-4 32-33 9.060274381349365E-5 34-35 2.1140640223148518E-4 36-37 0.0 38-39 0.0 40-41 1.510045730224894E-4 42-43 0.0 44-45 1.510045730224894E-4 46-47 0.0 48-49 3.624109752539746E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 1.812054876269873E-4 58-59 9.060274381349365E-5 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 6.0401829208995765E-5 70-71 0.0 72-73 9.060274381349365E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.812054876269873E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 1.812054876269873E-4 100-101 0.0 102-103 3.0200914604497883E-5 104-105 0.0012080365841799152 106-107 0.0 108-109 0.0016308493886428854 110-111 0.004469735361465686 112-113 0.0 114-115 0.0 116-117 3.0200914604497883E-5 118-119 0.0 120-121 7.248219505079492E-4 122-123 0.0011476347549709194 124-125 6.0401829208995765E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1655579.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.73108554251074 #Duplication Level Percentage of deduplicated Percentage of total 1 83.20225075176322 56.35378762997103 2 10.38087502998833 14.062158693245063 3 2.968702477401276 6.032203243413881 4 1.208957273274993 3.2753595397371633 5 0.6385533348627359 2.1624955273521738 6 0.35333394669994267 1.435901506140405 7 0.2274076345881413 1.0781796165916597 8 0.15637247022902817 0.8473021726060816 9 0.11659517475482864 0.7107405979646958 >10 0.6718728463339322 8.360325975715943 >50 0.054852602725503616 2.5481633300401936 >100 0.019497576041392423 2.186381961879968 >500 1.8222033417892428E-4 0.10009346624946525 >1k 5.466610025367728E-4 0.8469067390922693 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2724 0.16453458276530447 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2722 0.16441377910688645 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 6.0401829208995765E-5 0.0 0.0 0.0 6 0.0 6.0401829208995765E-5 0.0 0.0 0.0 7 0.0 6.0401829208995765E-5 0.0 0.0 0.0 8 0.0 6.0401829208995765E-5 0.0 0.0 0.0 9 0.0 6.0401829208995765E-5 0.0 0.0 0.0 10-11 0.0 6.0401829208995765E-5 0.0 0.0 0.0 12-13 0.0 6.0401829208995765E-5 0.0 0.0 0.0 14-15 0.0 6.0401829208995765E-5 0.0 0.0 0.0 16-17 0.0 6.0401829208995765E-5 0.0 6.0401829208995765E-5 0.0 18-19 6.0401829208995765E-5 6.0401829208995765E-5 0.0 6.0401829208995765E-5 0.0 20-21 1.2080365841799153E-4 6.0401829208995765E-5 0.0 6.0401829208995765E-5 0.0 22-23 1.2080365841799153E-4 6.0401829208995765E-5 0.0 1.5100457302248942E-4 0.0 24-25 1.2080365841799153E-4 6.0401829208995765E-5 0.0 1.812054876269873E-4 0.0 26-27 1.2080365841799153E-4 6.0401829208995765E-5 0.0 4.2281280446297036E-4 0.0 28-29 1.812054876269873E-4 6.0401829208995765E-5 0.0 0.002597278655986818 0.0 30-31 1.812054876269873E-4 9.060274381349365E-5 0.0 0.00842605517465491 0.0 32-33 1.812054876269873E-4 1.2080365841799153E-4 0.0 0.024221133512807302 0.0 34-35 1.812054876269873E-4 1.2080365841799153E-4 0.0 0.045271170992142326 0.0 36-37 2.4160731683598306E-4 1.2080365841799153E-4 0.0 0.08226729138265224 0.0 38-39 2.4160731683598306E-4 2.1140640223148518E-4 0.0 0.14046445382551964 0.0 40-41 2.4160731683598306E-4 2.7180823144048094E-4 0.0 0.20279914156920328 0.0 42-43 3.322100606494767E-4 3.0200914604497885E-4 0.0 0.2650734274836779 0.0 44-45 4.832146336719661E-4 3.0200914604497885E-4 0.0 0.3438978146014174 0.0 46-47 6.040182920899577E-4 3.0200914604497885E-4 0.0 0.4420507870660355 0.0 48-49 6.040182920899577E-4 3.3221006064947675E-4 0.0 0.5464553488537847 0.0 50-51 6.040182920899577E-4 3.9261188985847246E-4 0.0 0.6551484405153726 0.0 52-53 6.040182920899577E-4 4.2281280446297036E-4 0.0 0.756774518159508 0.0 54-55 6.040182920899577E-4 4.2281280446297036E-4 0.0 0.8737728613373328 0.0 56-57 6.040182920899577E-4 4.2281280446297036E-4 0.0 1.0146299270527108 0.0 58-59 6.040182920899577E-4 4.2281280446297036E-4 0.0 1.176959843051887 0.0 60-61 6.040182920899577E-4 4.2281280446297036E-4 0.0 1.35001108373566 0.0 62-63 6.040182920899577E-4 4.2281280446297036E-4 0.0 1.5171731460715556 0.0 64-65 6.040182920899577E-4 4.2281280446297036E-4 0.0 1.6907378023036048 0.0 66-67 6.040182920899577E-4 4.832146336719661E-4 0.0 1.8983992911241323 0.0 68-69 6.040182920899577E-4 4.832146336719661E-4 0.0 2.1290134750440783 0.0 70-71 6.342192066944555E-4 4.832146336719661E-4 0.0 2.380134079980478 0.0 72-73 6.946210359034513E-4 4.832146336719661E-4 0.0 2.63052986296637 0.0 74-75 7.248219505079492E-4 4.832146336719661E-4 0.0 2.8840061392419205 0.0 76-77 7.248219505079492E-4 4.832146336719661E-4 0.0 3.159649886837173 0.0 78-79 7.550228651124471E-4 4.832146336719661E-4 0.0 3.471746138360054 0.0 80-81 9.060274381349364E-4 4.832146336719661E-4 0.0 3.8095131672967586 0.0 82-83 9.060274381349364E-4 4.832146336719661E-4 0.0 4.169930882186836 0.0 84-85 9.966301819484301E-4 4.832146336719661E-4 0.0 4.5303183961623095 0.0 86-87 0.0010872329257619238 4.832146336719661E-4 0.0 4.8863267775201304 0.0 88-89 0.0010872329257619238 4.832146336719661E-4 0.0 5.285009051214107 0.0 90-91 0.0010872329257619238 4.832146336719661E-4 0.0 5.735818103515447 0.0 92-93 0.0010872329257619238 4.832146336719661E-4 0.0 6.1922747268478275 0.0 94-95 0.0010872329257619238 4.832146336719661E-4 0.0 6.653352090114697 0.0 96-97 0.0010872329257619238 4.832146336719661E-4 0.0 7.11720793752518 0.0 98-99 0.0011476347549709196 4.832146336719661E-4 0.0 7.595741429433449 0.0 100-101 0.001268438413388911 4.832146336719661E-4 0.0 8.135975389878707 0.0 102-103 0.0013288402425979068 4.832146336719661E-4 0.0 8.709822968278772 0.0 104-105 0.0016308493886428856 5.436164628809619E-4 0.0 9.272043194556105 0.0 106-107 0.0017214521324563792 5.436164628809619E-4 0.0 9.826743393096915 0.0 108-109 0.0017516530470608773 5.436164628809619E-4 0.0 10.396302441623142 0.0 110-111 0.0017516530470608773 5.738173774854598E-4 0.0 11.003733437063408 0.0 112-113 0.0017516530470608773 6.040182920899577E-4 0.0 11.659123484895616 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2400 0.0 72.95677 1 GTATCAA 4235 0.0 51.47036 1 ATCAACG 5040 0.0 42.417095 3 TCAACGC 5085 0.0 42.15883 4 CAACGCA 5125 0.0 41.713593 5 AACGCAG 5305 0.0 40.383636 6 TATCAAC 5345 0.0 40.219486 2 GTACATG 5480 0.0 33.799427 1 ACGCAGA 6345 0.0 33.663498 7 CGCAGAG 6450 0.0 33.299976 8 ACATGGG 5500 0.0 31.182203 3 TACATGG 5795 0.0 30.622444 2 GCAGAGT 7335 0.0 29.038847 9 CATGGGG 3720 0.0 27.053331 4 GAGTACT 4635 0.0 26.443195 12-13 CAGAGTA 7475 0.0 25.271381 10-11 GTACTTT 4840 0.0 25.261719 14-15 CAATACA 1180 0.0 23.718807 4 CCAATAC 1140 0.0 22.98396 3 ATGGGGA 2275 0.0 22.772717 5 >>END_MODULE