FastQCFastQC Report
Thu 26 May 2016
SRR1364384_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364384_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2333502
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16900.051.7935941
GTATCAA28450.044.7895741
TCAACGC29600.040.4204144
CAACGCA30800.038.8447575
ATCAACG30950.038.6581573
GTACATG76650.036.278571
AACGCAG33250.035.9778866
TACATGG77250.034.9834862
TAAGGTG19350.034.1451575
TATCAAC35900.033.9910932
ACATGGG80300.032.4686553
ACGCAGA41000.029.1678127
CGCAGAG40800.028.7268758
GGTAAGG19950.028.0472763
GTAAGGT20900.027.6262444
AGGTAAG18750.027.302532
CATGGGA44600.026.8261034
GTGTACG2705.820766E-1126.464451
TAAGGTA11900.025.5105384
CATGGGG41350.024.7599264
Produced by FastQC (version 0.11.5)