##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364384_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2333502 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.157029220459208 31.0 31.0 34.0 28.0 34.0 2 31.231677967278365 33.0 31.0 34.0 28.0 34.0 3 31.31556947454941 33.0 31.0 34.0 28.0 34.0 4 34.75217462851971 37.0 35.0 37.0 32.0 37.0 5 34.81965260796862 37.0 35.0 37.0 32.0 37.0 6 34.94720167370759 37.0 35.0 37.0 32.0 37.0 7 34.912765020128546 37.0 35.0 37.0 32.0 37.0 8 34.858449232098366 37.0 35.0 37.0 32.0 37.0 9 36.44615732062797 39.0 37.0 39.0 32.0 39.0 10-11 36.35476635546059 39.0 37.0 39.0 32.0 39.0 12-13 36.36020024838204 39.0 37.0 39.0 32.0 39.0 14-15 37.69299126377436 40.0 38.0 41.0 32.0 41.0 16-17 37.69494326552966 40.0 38.0 41.0 32.0 41.0 18-19 37.70610374450075 40.0 38.0 41.0 32.0 41.0 20-21 37.732339633735045 40.0 38.0 41.0 32.0 41.0 22-23 37.71748342191265 40.0 38.0 41.0 32.0 41.0 24-25 37.72077739809094 40.0 38.0 41.0 32.0 41.0 26-27 37.678063485696605 40.0 38.0 41.0 32.0 41.0 28-29 37.687093475814464 40.0 38.0 41.0 32.0 41.0 30-31 37.58480794102598 40.0 38.0 41.0 31.5 41.0 32-33 37.5379637557628 40.0 38.0 41.0 31.0 41.0 34-35 37.53385405283561 40.0 38.0 41.0 31.0 41.0 36-37 37.47323636320003 40.0 38.0 41.0 31.0 41.0 38-39 37.38042714340935 40.0 38.0 41.0 31.0 41.0 40-41 37.320950656995365 40.0 37.0 41.0 31.0 41.0 42-43 37.190172538956475 40.0 37.0 41.0 30.0 41.0 44-45 37.07433955488361 40.0 37.0 41.0 30.0 41.0 46-47 36.970361071042575 40.0 37.0 41.0 30.0 41.0 48-49 36.81603486947944 40.0 36.0 41.0 30.0 41.0 50-51 36.69947143820747 40.0 36.0 41.0 30.0 41.0 52-53 36.53504432393887 40.0 36.0 41.0 30.0 41.0 54-55 36.34999348618514 39.0 35.0 41.0 30.0 41.0 56-57 36.16106092902427 39.0 35.0 41.0 29.0 41.0 58-59 35.938424736726176 39.0 35.0 41.0 29.0 41.0 60-61 35.69366771487661 38.5 35.0 41.0 29.0 41.0 62-63 35.42860130396288 38.0 35.0 40.0 28.5 41.0 64-65 35.079152706961466 37.5 34.0 40.0 28.0 41.0 66-67 34.77165972002595 37.0 34.0 40.0 28.0 41.0 68-69 34.44322996080569 36.5 34.0 39.0 28.0 41.0 70-71 34.07258575308699 36.0 34.0 39.0 27.0 41.0 72-73 33.71818279992903 35.5 33.5 39.0 27.0 40.0 74-75 33.36038109245246 35.0 33.0 37.5 26.0 40.0 76-77 33.12007981994444 35.0 33.0 37.0 26.5 39.0 78-79 32.942067330561535 35.0 33.0 37.0 26.5 39.0 80-81 32.67888135514775 35.0 33.0 36.0 26.0 39.0 82-83 32.37784840124414 35.0 33.0 36.0 26.0 37.0 84-85 32.12687625723055 35.0 33.0 35.5 26.0 37.0 86-87 31.92670929787075 35.0 33.0 35.0 25.5 36.5 88-89 31.659786663992573 35.0 33.0 35.0 25.0 36.0 90-91 31.529057828105568 35.0 33.0 35.0 25.0 36.0 92-93 31.384974171867004 35.0 33.0 35.0 24.5 36.0 94-95 31.272284746274053 35.0 33.0 35.0 24.0 35.5 96-97 31.164343120340156 35.0 32.0 35.0 24.0 35.0 98-99 31.022916200628927 35.0 32.0 35.0 23.0 35.0 100-101 30.924047847398462 35.0 32.0 35.0 21.5 35.0 102-103 29.90743611961764 33.5 30.0 34.5 19.0 35.0 104-105 30.603078548893464 34.5 32.0 35.0 19.5 35.0 106-107 30.92463151949302 35.0 32.5 35.0 22.0 35.0 108-109 30.96005253048851 35.0 33.0 35.0 21.5 35.0 110-111 30.874455003681163 35.0 33.0 35.0 19.5 35.0 112-113 30.771606795280228 35.0 33.0 35.0 19.0 35.0 114-115 30.660164850940774 35.0 33.0 35.0 18.0 35.0 116-117 30.52719946243886 35.0 32.5 35.0 12.5 35.0 118-119 30.342795077955792 35.0 32.0 35.0 3.5 35.0 120-121 30.155763526236534 35.0 32.0 35.0 2.0 35.0 122-123 29.96806687973698 35.0 32.0 35.0 2.0 35.0 124-125 29.166056639334357 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1873772823969837 1101 2 0.08568525972449592 1101 3 0.1851602785751716 1101 4 0.35743654535448144 1101 5 0.19688636175937546 1101 6 0.25485631676595233 1101 7 0.3188033522572411 1101 8 0.17709728482881104 1101 9 0.37169804076345514 1101 10-11 0.45411516131102303 1101 12-13 0.5308049086610467 1101 14-15 0.5689642329435856 1101 16-17 0.5225752585672012 1101 18-19 0.38029751417094104 1101 20-21 0.43017963749747423 1101 22-23 0.6016715774995092 1101 24-25 0.4740905757389484 1101 26-27 0.6249057180731086 1101 28-29 0.5649569748933061 1101 30-31 0.538641936962371 1101 32-33 0.44239592218770696 1101 34-35 0.49022390331717247 1101 36-37 0.43036693911523827 1101 38-39 0.5835285331429461 1101 40-41 0.4958050050440974 1101 42-43 0.6098351359770291 1101 44-45 0.5933993126595141 1101 46-47 0.5170812621640408 1101 48-49 0.43095202869626803 1101 50-51 0.4412612458048102 1101 52-53 0.4306240795687728 1101 54-55 0.4638954103535795 1101 56-57 0.42992594426140585 1101 58-59 0.4796794496162562 1101 60-61 0.36313650478632553 1101 62-63 0.4129010925754102 1101 64-65 0.5027552350418816 1101 66-67 0.47471102569404877 1101 68-69 0.4201825817556184 1101 70-71 0.4564803407185636 1101 72-73 0.443204097580292 1101 74-75 0.41745534589126265 1101 76-77 0.4962460042203176 1101 78-79 0.48615574661783256 1101 80-81 0.5806864597488044 1101 82-83 0.44589339436013375 1101 84-85 0.5101423204656896 1101 86-87 0.43301089671408377 1101 88-89 0.41087955432750434 1101 90-91 0.3751306537443888 1101 92-93 0.4011262888603184 1101 94-95 0.36649128966574906 1101 96-97 0.296689817162882 1101 98-99 0.2915923940670524 1101 100-101 0.27777902206310756 1101 102-103 0.07991244008279708 1101 104-105 0.20273810199372022 1101 106-107 0.1983012113584337 1101 108-109 0.23662878440811141 1101 110-111 0.22050651153074696 1101 112-113 0.17937092772258367 1101 114-115 0.10272678999700346 1101 116-117 0.09413419860467087 1101 118-119 0.19016786353405024 1101 120-121 0.01968969300564183 1101 122-123 0.030583902722728595 1101 124-125 -0.0628533592269882 1102 1 0.2523743031648493 1102 2 0.2525181582485132 1102 3 0.19687142228862697 1102 4 0.40805314342316024 1102 5 0.33519837567473587 1102 6 0.4212902223779693 1102 7 0.3695446242189391 1102 8 0.4461505634149958 1102 9 0.5351086044698974 1102 10-11 0.5447495728001215 1102 12-13 0.4395161870733446 1102 14-15 0.5342693021221052 1102 16-17 0.55966592401046 1102 18-19 0.5228147657386089 1102 20-21 0.5146205452619483 1102 22-23 0.528011771724664 1102 24-25 0.5208411439256366 1102 26-27 0.5181187921357804 1102 28-29 0.44705297452001247 1102 30-31 0.4870116799937989 1102 32-33 0.5694609018926613 1102 34-35 0.41813641942771795 1102 36-37 0.2811923021595959 1102 38-39 0.30302526072666325 1102 40-41 0.3431695739266942 1102 42-43 0.311712562583935 1102 44-45 0.24036504356112687 1102 46-47 0.2957767175636903 1102 48-49 0.3175527840669474 1102 50-51 0.23967718561721796 1102 52-53 0.27305441484129744 1102 54-55 0.3039916188637335 1102 56-57 0.2567316697094455 1102 58-59 0.22621273162186384 1102 60-61 0.2767873026901384 1102 62-63 0.2707918108045675 1102 64-65 0.23093657467744322 1102 66-67 0.13874705499095086 1102 68-69 0.13931490249634493 1102 70-71 0.058865221056230155 1102 72-73 0.22637646671053346 1102 74-75 0.20738288921589287 1102 76-77 0.22154580939002244 1102 78-79 0.34811053301080364 1102 80-81 0.3445303654829459 1102 82-83 0.2829947864972624 1102 84-85 0.2906566658922145 1102 86-87 0.17182004777782467 1102 88-89 0.2666539605635272 1102 90-91 0.2524345917442794 1102 92-93 0.22903521421693185 1102 94-95 0.06477691427375376 1102 96-97 0.0918828274778285 1102 98-99 0.05927348517505138 1102 100-101 0.010148237964568096 1102 102-103 -0.19944224675333544 1102 104-105 -0.0934229537098119 1102 106-107 -0.08604585378349228 1102 108-109 -0.07904574054152391 1102 110-111 -0.05923021694245634 1102 112-113 -0.09841635856603759 1102 114-115 -0.1268417289701027 1102 116-117 -0.16809938157932436 1102 118-119 -0.19224811419088184 1102 120-121 -0.28272483383597447 1102 122-123 -0.34742155376508066 1102 124-125 -0.1901349689346361 1103 1 0.2149961500838664 1103 2 0.31081620535799104 1103 3 0.27720043943452666 1103 4 0.3069765487731928 1103 5 0.32639163827013107 1103 6 0.3312823241611653 1103 7 0.2620423111697292 1103 8 0.3722589088965407 1103 9 0.4462228848972174 1103 10-11 0.4777434934298128 1103 12-13 0.39919127700017754 1103 14-15 0.4398612847550538 1103 16-17 0.3439977123021478 1103 18-19 0.3830452582714159 1103 20-21 0.45151275491221554 1103 22-23 0.45768176166007635 1103 24-25 0.48526202410291575 1103 26-27 0.43021425675402725 1103 28-29 0.36286940190831984 1103 30-31 0.49815944886038466 1103 32-33 0.4934128987875326 1103 34-35 0.5785030320856279 1103 36-37 0.48451230631180664 1103 38-39 0.4036364924422884 1103 40-41 0.3994275793335902 1103 42-43 0.3137163627545405 1103 44-45 0.3083653504632622 1103 46-47 0.269044142158279 1103 48-49 0.2634313140057003 1103 50-51 0.26471936209497215 1103 52-53 0.3565169479573953 1103 54-55 0.25486308554003756 1103 56-57 0.2255598902637601 1103 58-59 0.10731478109629222 1103 60-61 0.05261596103132149 1103 62-63 0.1174485181959426 1103 64-65 0.16881867912346138 1103 66-67 0.14825380006810462 1103 68-69 0.10656090712695487 1103 70-71 0.09880659372117861 1103 72-73 0.09285313989134636 1103 74-75 0.07907563504225834 1103 76-77 0.1475722842127638 1103 78-79 0.17331983575622445 1103 80-81 0.161635781854379 1103 82-83 0.11931489445459675 1103 84-85 0.1037746110921276 1103 86-87 0.14231240004732726 1103 88-89 0.20594140600941913 1103 90-91 0.17444376270231032 1103 92-93 0.18787977279687595 1103 94-95 0.23215267532335915 1103 96-97 0.1664997188299573 1103 98-99 0.1907488720748418 1103 100-101 0.0996856732556104 1103 102-103 -0.03889541935419416 1103 104-105 0.09110845637011877 1103 106-107 0.07461719023439528 1103 108-109 0.11096910810607596 1103 110-111 0.12420690648185229 1103 112-113 0.10516636284354419 1103 114-115 0.05850315230841474 1103 116-117 0.10332297443618543 1103 118-119 0.09987718312056515 1103 120-121 -0.15419864109240322 1103 122-123 -0.13994392828498192 1103 124-125 -0.15109083369911502 1104 1 0.18375884601002923 1104 2 0.24738323583712685 1104 3 0.2746960607605615 1104 4 0.3320813026983984 1104 5 0.27597828253462353 1104 6 0.2952400214911748 1104 7 0.3537705615273765 1104 8 0.3872676216778146 1104 9 0.45456237585686665 1104 10-11 0.5301061501373141 1104 12-13 0.4028602269651955 1104 14-15 0.4536467765258223 1104 16-17 0.4465844947232753 1104 18-19 0.3893520194788138 1104 20-21 0.3640912925019606 1104 22-23 0.35890935020288595 1104 24-25 0.4306928722509511 1104 26-27 0.44608640393095556 1104 28-29 0.380510176833333 1104 30-31 0.3224710054399864 1104 32-33 0.396091176553675 1104 34-35 0.4165572073128061 1104 36-37 0.34177702779248875 1104 38-39 0.25756566711238804 1104 40-41 0.30648733876277845 1104 42-43 0.2086198982273686 1104 44-45 0.29877986493183073 1104 46-47 0.2704670217881997 1104 48-49 0.16283536868007786 1104 50-51 0.14199397737331765 1104 52-53 0.04341403909175057 1104 54-55 -0.002489209419927363 1104 56-57 -0.12128724164908533 1104 58-59 -0.05241567976749906 1104 60-61 -0.10391577265616547 1104 62-63 -0.027647857197905523 1104 64-65 0.026019536585920378 1104 66-67 0.06537630934911931 1104 68-69 0.002370259885402959 1104 70-71 -0.022215899070168632 1104 72-73 -0.08247471471439383 1104 74-75 -0.050527008651783945 1104 76-77 -0.03941077600124032 1104 78-79 0.0788559057793563 1104 80-81 0.09470731579411051 1104 82-83 0.05019526417958531 1104 84-85 0.019195520875847194 1104 86-87 0.04425176265501207 1104 88-89 -0.025503756948559442 1104 90-91 -0.10601888375486368 1104 92-93 -0.1442804621585374 1104 94-95 -0.013137565024617714 1104 96-97 -0.08750361475592072 1104 98-99 -0.14435490138393092 1104 100-101 -0.11006524370334247 1104 102-103 -0.3430468033717027 1104 104-105 -0.14845104680079757 1104 106-107 -0.16096727917727804 1104 108-109 -0.1780403088283613 1104 110-111 -0.20931595398440805 1104 112-113 -0.2206285503183274 1104 114-115 -0.30212470274299363 1104 116-117 -0.3398922424028825 1104 118-119 -0.33488333795277825 1104 120-121 -0.36708437664278293 1104 122-123 -0.42126100667327293 1104 124-125 -0.5268581155341892 1105 1 2.1275413842403168E-4 1105 2 0.03893090397375687 1105 3 -0.11823175334308189 1105 4 0.020554746221321807 1105 5 0.13124153842920094 1105 6 0.22034398862794546 1105 7 0.35324750966703533 1105 8 0.2863677862783405 1105 9 0.2780386360733047 1105 10-11 0.30313135006500147 1105 12-13 0.4463449643457622 1105 14-15 0.4309795031867054 1105 16-17 0.4721899145561821 1105 18-19 0.4117870087029232 1105 20-21 0.43163095642167093 1105 22-23 0.4084775277276975 1105 24-25 0.36476087339387675 1105 26-27 0.28516433628832516 1105 28-29 0.30644819163316583 1105 30-31 0.3621748311326982 1105 32-33 0.41365657834891323 1105 34-35 0.33104525295407683 1105 36-37 0.23370156410447862 1105 38-39 0.2911173703655976 1105 40-41 0.3259361081182348 1105 42-43 0.2887205086915188 1105 44-45 0.3027300913845039 1105 46-47 0.2723860211984501 1105 48-49 0.20021025941471038 1105 50-51 0.27543415547501127 1105 52-53 0.3359761993196315 1105 54-55 0.27707532366910925 1105 56-57 0.20195047137182343 1105 58-59 0.267984223002415 1105 60-61 0.2296313094592506 1105 62-63 0.21896254669292148 1105 64-65 0.23378762690157373 1105 66-67 0.11029901616449678 1105 68-69 0.061871852400578575 1105 70-71 0.129750598188636 1105 72-73 0.07281100363385917 1105 74-75 0.06249036411796283 1105 76-77 0.16053028333281105 1105 78-79 0.29988640765861163 1105 80-81 0.16306181495418315 1105 82-83 0.15396122064677087 1105 84-85 0.15010579530710544 1105 86-87 0.1062022196567618 1105 88-89 0.07528761713068022 1105 90-91 0.18378438950029619 1105 92-93 0.12623679719867553 1105 94-95 0.1405734963881038 1105 96-97 0.08967863716345903 1105 98-99 0.09713539185688447 1105 100-101 -0.010111345054202303 1105 102-103 -0.10640354200624103 1105 104-105 0.04340158257582161 1105 106-107 0.012849921137704001 1105 108-109 0.015694630155689282 1105 110-111 -0.029473754837404442 1105 112-113 0.0022795215398581092 1105 114-115 -0.0064916864231001625 1105 116-117 0.010307524475106078 1105 118-119 0.08480103325763366 1105 120-121 -0.09627050964082073 1105 122-123 -0.12412777097629046 1105 124-125 -0.09025081610003127 1106 1 0.26596422462271363 1106 2 0.2945876597959938 1106 3 0.32204504538539425 1106 4 0.39712026993151284 1106 5 0.31152033346307917 1106 6 0.2756509611782647 1106 7 0.3401779178876154 1106 8 0.40771473842622896 1106 9 0.48806238160072013 1106 10-11 0.5615695610697031 1106 12-13 0.39817382843843774 1106 14-15 0.5508227788850846 1106 16-17 0.5477827886055451 1106 18-19 0.5246898994295677 1106 20-21 0.40403024329171444 1106 22-23 0.5603040755330042 1106 24-25 0.5041305710061437 1106 26-27 0.5262888961937264 1106 28-29 0.5224707632565853 1106 30-31 0.5518819903232455 1106 32-33 0.5052984583528044 1106 34-35 0.4923574751189861 1106 36-37 0.5456789666914545 1106 38-39 0.43293779553088996 1106 40-41 0.43641119537301876 1106 42-43 0.45448036079299214 1106 44-45 0.4437143845682243 1106 46-47 0.40118055361406135 1106 48-49 0.3862951749872323 1106 50-51 0.3508028033553927 1106 52-53 0.37090235034730057 1106 54-55 0.29815664302640243 1106 56-57 0.2737796172396614 1106 58-59 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-0.8743290182402994 2216 98-99 -0.9134198357437953 2216 100-101 -0.8675158633353455 2216 102-103 -0.8805384439008854 2216 104-105 -0.9478462388668021 2216 106-107 -0.7076582683639927 2216 108-109 -0.6968477697243678 2216 110-111 -0.7207204050126244 2216 112-113 -0.6442692219244712 2216 114-115 -0.6540641175994253 2216 116-117 -0.7549843085115313 2216 118-119 -0.6724978367035703 2216 120-121 -0.49502966895192557 2216 122-123 -0.5599338840551304 2216 124-125 -0.4268889975434078 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 30183.0 3 5881.0 4 3687.0 5 3035.0 6 3407.0 7 4231.0 8 4710.0 9 5344.0 10 6065.0 11 6826.0 12 7679.0 13 7885.0 14 8188.0 15 7578.0 16 7066.0 17 6308.0 18 5997.0 19 6015.0 20 6067.0 21 6407.0 22 7762.0 23 10026.0 24 14412.0 25 20179.0 26 23701.0 27 25430.0 28 30115.0 29 36503.0 30 43253.0 31 53863.0 32 69449.0 33 92267.0 34 128334.0 35 195130.0 36 380995.0 37 730386.0 38 328030.0 39 1108.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.119123657483684 18.540755346163525 12.466328569707525 24.873792426645263 2 15.568495801420227 19.957407762176054 39.42533479564683 25.04876164075689 3 18.508112676225345 24.53366200303671 29.871387693828055 27.086837626909887 4 12.194316041920413 16.424156607556267 38.13456394045704 33.246963410066286 5 12.919724517977185 38.63863838119377 35.41265079220156 13.02898630862748 6 32.412793875263354 37.33508799379296 17.320213388430812 12.931904742512865 7 28.281621837751942 32.644646693468445 21.63834566069562 17.43538580808399 8 26.587529462181276 35.02176987358046 20.349303621169916 18.04139704306835 9 26.53719602554444 15.656082574602465 19.770670862934765 38.03605053691833 10-11 24.467671837941253 26.518025823446717 28.591435309928286 20.422867028683743 12-13 25.241175469805228 24.054400597908607 28.025958798645963 22.678465133640202 14-15 22.205175762333877 25.72717198087673 25.125134348007194 26.942517908782204 16-17 22.547106298309963 28.81837532392343 26.44408675555464 22.190431622211964 18-19 22.73360928493281 27.720558079897973 27.379632577830478 22.166200057338735 20-21 22.91830047713694 27.17887107017693 27.407968795398503 22.49485965728763 22-23 22.843755865647424 27.239230992731095 27.292605705930402 22.624407435691076 24-25 22.699487722744614 27.39440549011743 27.241995078641455 22.664111708496502 26-27 22.2038425944873 27.655799664152774 27.02948766049647 23.110870080863457 28-29 22.62199741846126 27.947171020942 26.854945292291553 22.575886268305183 30-31 22.4904462078918 27.391565664844297 26.85723897282941 23.26074915443449 32-33 22.153960343638754 27.60899199784701 26.799841611109848 23.437206047404388 34-35 23.234597544829434 27.619008233827238 26.55334824523079 22.59304597611254 36-37 23.411199964345382 26.898560251913594 26.55019919661401 23.14004058712701 38-39 22.57773730447825 27.3785301049925 26.701349903578315 23.342382686950934 40-41 23.05286037590953 27.069017311859888 26.967988251059467 22.910134061171114 42-43 22.73656337108308 27.103300368997996 26.722731677967023 23.437404581951903 44-45 22.58425878071504 27.502200589166502 27.128721824102005 22.784818806016457 46-47 23.38423108272459 27.70702574928155 26.15037398725178 22.75836918074208 48-49 22.73321385625553 27.642016162831656 26.34251009855573 23.282259882357074 50-51 22.883867251881508 27.60496884082379 26.678164406972865 22.832999500321836 52-53 23.191422071012486 27.220345433016913 26.74069639426487 22.84753610170574 54-55 22.391582162879818 27.586347285064967 26.970621738165256 23.051448813889962 56-57 22.589486937416247 27.86536687212368 26.743724118779667 22.8014220716804 58-59 22.45339578655056 27.092004216877797 26.95071309803384 23.503886898537807 60-61 22.013418188199612 27.449346457396473 27.255665317962546 23.28157003644137 62-63 22.320651833872017 27.417871520537158 27.443177094448718 22.818299551142115 64-65 22.10117188412337 27.538173389824443 27.00441768023469 23.356237045817497 66-67 22.100430223005425 27.663502098403175 26.941971106190387 23.294096572401017 68-69 22.7348932169149 27.022330294305956 27.36862427799172 22.87415221078742 70-71 22.54535971935214 27.036698324975205 26.88077094166683 23.53717101400582 72-73 22.33105321790691 27.310087341213134 26.955909840194785 23.40294960068517 74-75 22.377809680944523 27.62287599905719 26.89712657224282 23.10218774775547 76-77 22.662773458617664 27.215979837713476 27.117049727111887 23.00419697655697 78-79 22.790784082391628 27.282776809012955 27.029931333168484 22.896507775426933 80-81 22.972278611800714 27.3672802167074 26.81179822830301 22.848642943188874 82-83 22.74261265241701 27.498250469362752 26.939857714840866 22.819279163379377 84-85 22.52383344061203 27.656604753574694 27.035366828856056 22.784194976957224 86-87 22.885521395648123 27.522146895149184 26.789391774895023 22.80293993430767 88-89 22.753902190352232 27.615177882280946 26.547815626246855 23.08310430111997 90-91 22.48365965456004 27.80012153480112 26.85518417064926 22.861034639989576 92-93 22.752813021418454 27.169459671473295 27.247369110107506 22.830358197000745 94-95 22.36661326997726 26.97689886084206 27.490724146531488 23.165763722649196 96-97 22.898534452483645 26.49200459482155 27.5017339858877 23.107726966807107 98-99 22.509418332525527 26.948665962996916 27.082414641473402 23.459501063004158 100-101 22.39229335906899 26.803474485186857 27.504576878705855 23.299655277038298 102-103 22.138424749407534 26.54208110667804 27.72332620517941 23.596167938735015 104-105 22.12225718427286 26.90356580775729 27.207832070735478 23.766344937234372 106-107 22.381333399618743 26.93745542877518 26.84104872663439 23.84016244497168 108-109 22.384171406433744 26.69888079785793 27.06398428420089 23.852963511507436 110-111 22.36285979556971 26.982800865924233 27.266456835245336 23.387882503260716 112-113 22.42738630110686 27.22075181577102 27.38795102860836 22.96391085451376 114-115 22.38719875616555 27.484824375452526 27.02970027592434 23.09827659245758 116-117 22.286023575311834 27.3017984467687 27.24032315987689 23.17185481804258 118-119 22.02594215397438 27.608776694550187 26.88642488375079 23.47885626772464 120-121 22.095656605182278 27.782258945894966 27.056138274085477 23.065946174837272 122-123 22.103330584845143 27.824543028125433 27.04917365359792 23.022952733431502 124-125 22.00655598320249 27.775461218003798 27.21840835058611 22.999574448207603 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 450.0 1 407.0 2 336.5 3 289.5 4 256.0 5 227.0 6 205.5 7 187.5 8 172.5 9 172.0 10 169.5 11 160.0 12 160.5 13 175.0 14 190.0 15 193.5 16 230.0 17 308.5 18 390.5 19 534.0 20 741.5 21 1159.5 22 1729.0 23 2510.0 24 3827.5 25 5876.0 26 8928.5 27 12771.0 28 16562.5 29 20656.0 30 25535.0 31 32164.5 32 43286.0 33 48032.5 34 47310.0 35 51123.5 36 57716.0 37 63661.5 38 67361.5 39 71457.0 40 74775.0 41 78170.0 42 79826.5 43 79915.0 44 81192.5 45 86159.0 46 88357.5 47 87428.0 48 86087.5 49 83057.5 50 81491.5 51 79239.5 52 78843.5 53 81714.5 54 84042.5 55 79973.5 56 72876.0 57 66801.0 58 62036.5 59 54089.5 60 41708.5 61 34126.5 62 29855.5 63 21776.5 64 13867.0 65 10031.0 66 7411.0 67 5953.0 68 4722.5 69 3327.0 70 2314.0 71 1503.5 72 1047.5 73 802.5 74 527.0 75 332.5 76 233.5 77 133.0 78 85.5 79 60.0 80 39.5 81 25.5 82 20.5 83 20.5 84 14.5 85 8.0 86 9.0 87 7.5 88 4.0 89 9.0 90 11.5 91 7.0 92 5.5 93 6.0 94 4.5 95 3.0 96 2.5 97 2.0 98 2.0 99 1.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04435393670114703 2 0.009127911610960692 3 0.0023569724817034655 4 0.005485317775600792 5 0.024555367854837924 6 0.028583648096294757 7 0.003214053384141089 8 8.570809024376237E-5 9 0.0 10-11 1.0713511280470298E-4 12-13 0.0014998915792658416 14-15 4.285404512188119E-4 16-17 1.285621353656436E-4 18-19 4.285404512188119E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 2.1427022560940593E-5 28-29 4.285404512188119E-4 30-31 1.9284320304846535E-4 32-33 2.1427022560940596E-4 34-35 7.92799834754802E-4 36-37 1.4998915792658417E-4 38-39 8.570809024376237E-5 40-41 6.428106768282179E-4 42-43 8.785079249985644E-4 44-45 0.0015427456243877228 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0010713511280470297 54-55 0.0032569074292629705 56-57 2.1427022560940596E-4 58-59 9.427889926813863E-4 60-61 0.0011784862408517327 62-63 0.0010284970829251487 64-65 0.0016070266920705444 66-67 0.0026140967524347527 68-69 0.0034283236097504954 70-71 0.0018855779853627725 72-73 0.00289264804572698 74-75 0.002228410346337822 76-77 0.0015641726469486632 78-79 0.0026140967524347527 80-81 0.0014141834890220792 82-83 8.142268573157426E-4 84-85 6.85664721950099E-4 86-87 0.0020784211884112376 88-89 0.005699588001210198 90-91 5.999566317063367E-4 92-93 0.001007070060364208 94-95 0.0010284970829251487 96-97 2.3569724817034657E-4 98-99 8.999349475595051E-4 100-101 0.0014570375341439604 102-103 0.001371329443900198 104-105 8.356538798766832E-4 106-107 0.0067709391292572274 108-109 0.0023141184365815842 110-111 0.0017784428725580693 112-113 0.002485534617069109 114-115 0.0031069182713363867 116-117 0.0018855779853627725 118-119 0.048017957559067875 120-121 0.012041986679248615 122-123 0.004199696421944356 124-125 0.0026140967524347527 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2333502.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.56728058659604 #Duplication Level Percentage of deduplicated Percentage of total 1 81.56884026304442 51.85109356124178 2 10.408284678456528 13.232527051612294 3 3.4965330993249415 6.667953018153265 4 1.6168275521401498 4.111093226681286 5 0.8138304546666694 2.58664944308566 6 0.5043023297700006 1.9234276618178225 7 0.319416299168733 1.421309785923367 8 0.21824637695570664 1.109866294476113 9 0.1529040687302051 0.8747726255824602 >10 0.8010706623635601 9.301884832151403 >50 0.06432951113320058 2.828049166461448 >100 0.035080603296664924 3.9620345900819753 >500 3.3410094932516854E-4 0.12933874273110665 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 2.1427022560940593E-5 14-15 0.0 0.0 0.0 0.0 4.285404512188119E-5 16-17 0.0 0.0 0.0 2.1427022560940593E-5 4.285404512188119E-5 18-19 0.0 0.0 0.0 4.285404512188119E-5 4.285404512188119E-5 20-21 0.0 0.0 0.0 1.0713511280470297E-4 4.285404512188119E-5 22-23 0.0 0.0 0.0 1.9284320304846535E-4 4.285404512188119E-5 24-25 0.0 0.0 0.0 4.071134286578713E-4 4.285404512188119E-5 26-27 0.0 0.0 0.0 9.642160152423268E-4 8.570809024376237E-5 28-29 0.0 0.0 0.0 0.003149772316458267 8.570809024376237E-5 30-31 0.0 0.0 0.0 0.012363392017662723 8.570809024376237E-5 32-33 0.0 0.0 0.0 0.0331476039017751 8.570809024376237E-5 34-35 0.0 0.0 0.0 0.06316686250965287 1.2856213536564356E-4 36-37 0.0 0.0 0.0 0.11052058236933159 1.2856213536564356E-4 38-39 0.0 0.0 0.0 0.1937645650185858 1.2856213536564356E-4 40-41 0.0 0.0 0.0 0.2805011523452733 1.2856213536564356E-4 42-43 0.0 0.0 0.0 0.3666377830402545 1.2856213536564356E-4 44-45 0.0 0.0 0.0 0.47171590167910715 1.4998915792658414E-4 46-47 0.0 0.0 0.0 0.6029778418874293 1.7141618048752475E-4 48-49 0.0 0.0 0.0 0.7409250131347649 1.7141618048752475E-4 50-51 0.0 0.0 0.0 0.8980493695741423 2.1427022560940596E-4 52-53 0.0 0.0 0.0 1.04561727395134 2.1427022560940596E-4 54-55 0.0 0.0 0.0 1.201498863082183 2.1427022560940596E-4 56-57 0.0 0.0 0.0 1.3850427383392 2.1427022560940596E-4 58-59 0.0 0.0 0.0 1.5872066962016746 2.1427022560940596E-4 60-61 0.0 0.0 0.0 1.812426130339721 2.1427022560940596E-4 62-63 0.0 0.0 0.0 2.0344743651387485 2.571242707312871E-4 64-65 0.0 0.0 0.0 2.252151487335344 2.571242707312871E-4 66-67 0.0 0.0 0.0 2.5130040599922348 2.571242707312871E-4 68-69 0.0 0.0 0.0 2.805032950475294 2.571242707312871E-4 70-71 0.0 0.0 0.0 3.1197959118955114 2.571242707312871E-4 72-73 0.0 0.0 0.0 3.4402798883395 2.999783158531683E-4 74-75 0.0 0.0 0.0 3.7511645586761873 3.642593835359901E-4 76-77 0.0 0.0 0.0 4.0983894592762296 3.856864060969307E-4 78-79 0.0 0.0 0.0 4.492603820352414 3.856864060969307E-4 80-81 0.0 0.0 0.0 4.920608596007202 3.856864060969307E-4 82-83 0.0 0.0 0.0 5.365390730327208 4.713944963406931E-4 84-85 0.0 0.0 0.0 5.800916390900886 4.713944963406931E-4 86-87 0.0 0.0 0.0 6.240298915535535 4.928215189016336E-4 88-89 0.0 0.0 0.0 6.708436504446965 5.142485414625742E-4 90-91 0.0 0.0 0.0 7.237341129341222 5.142485414625742E-4 92-93 0.0 0.0 0.0 7.776209319726317 5.571025865844555E-4 94-95 0.0 0.0 0.0 8.309035089749226 5.571025865844555E-4 96-97 0.0 0.0 0.0 8.85424567881236 7.070917445110396E-4 98-99 0.0 0.0 0.0 9.41396236214925 7.285187670719802E-4 100-101 0.0 0.0 0.0 10.047452284163459 7.285187670719802E-4 102-103 0.0 0.0 0.0 10.7112614431014 7.285187670719802E-4 104-105 0.0 0.0 0.0 11.377170450250311 7.285187670719802E-4 106-107 0.0 0.0 0.0 12.027480584974857 7.285187670719802E-4 108-109 0.0 2.1427022560940593E-5 0.0 12.709888399495693 7.285187670719802E-4 110-111 0.0 4.285404512188119E-5 0.0 13.434807426777436 7.285187670719802E-4 112-113 0.0 4.285404512188119E-5 0.0 14.200609213105453 7.285187670719802E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1690 0.0 51.793594 1 GTATCAA 2845 0.0 44.789574 1 TCAACGC 2960 0.0 40.420414 4 CAACGCA 3080 0.0 38.844757 5 ATCAACG 3095 0.0 38.658157 3 GTACATG 7665 0.0 36.27857 1 AACGCAG 3325 0.0 35.977886 6 TACATGG 7725 0.0 34.983486 2 TAAGGTG 1935 0.0 34.145157 5 TATCAAC 3590 0.0 33.991093 2 ACATGGG 8030 0.0 32.468655 3 ACGCAGA 4100 0.0 29.167812 7 CGCAGAG 4080 0.0 28.726875 8 GGTAAGG 1995 0.0 28.047276 3 GTAAGGT 2090 0.0 27.626244 4 AGGTAAG 1875 0.0 27.30253 2 CATGGGA 4460 0.0 26.826103 4 GTGTACG 270 5.820766E-11 26.46445 1 TAAGGTA 1190 0.0 25.510538 4 CATGGGG 4135 0.0 24.759926 4 >>END_MODULE