FastQCFastQC Report
Thu 26 May 2016
SRR1364384_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364384_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2333502
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33880.14518950487293347No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33410.14317536475220505No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32150.076.518431
GTATCAA63050.051.7717061
TCAACGC75600.042.773284
CAACGCA76950.042.1002665
ATCAACG77350.041.959543
TATCAAC79800.040.820562
AACGCAG79950.040.4182936
ACGCAGA93100.034.7650957
CGCAGAG94500.033.998228
GTACATG78150.033.5367281
TACATGG78850.032.551432
TGGTATC14000.031.9027212
ACATGGG76750.031.8902553
TAAGGTG19000.030.7161675
GCAGAGT111450.028.2937189
GTGGTAT18500.027.0460931
GGTAAGG19450.026.6375453
GTAAGGT20150.026.3032574
CAGAGTA104300.025.98353810-11
GAGTACT71000.025.72611612-13
Produced by FastQC (version 0.11.5)