FastQCFastQC Report
Thu 26 May 2016
SRR1364383_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364383_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1748653
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14750.052.0740551
GTATCAA22450.045.0875131
ATCAACG23650.039.2582363
TCAACGC24250.038.041474
CAACGCA24550.037.5766035
GTACATG62900.036.6339721
AACGCAG25850.035.6817676
ACATGGG63850.034.6752323
TACATGG66150.034.5502432
TATCAAC27550.034.1338462
CATGGGA33650.028.2990744
ACGCAGA32700.028.1990817
GTGTAAG10050.027.8455311
CGCAGAG33950.027.16168
CATGGGG37350.026.133084
GAGTACA44350.025.1056631
TAAGGTG13200.024.7985385
GGACCGA5100.023.3364646
AGGTAAG10500.023.2404372
TAAGGTA8000.023.062644
Produced by FastQC (version 0.11.5)