##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364383_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1748653 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.146227982338406 33.0 31.0 34.0 28.0 34.0 2 31.118293337786284 33.0 31.0 34.0 28.0 34.0 3 31.26209430916254 33.0 31.0 34.0 28.0 34.0 4 34.695553091436665 37.0 35.0 37.0 32.0 37.0 5 34.7319674057689 37.0 35.0 37.0 32.0 37.0 6 34.7965628400832 37.0 35.0 37.0 32.0 37.0 7 34.70159374101094 37.0 35.0 37.0 32.0 37.0 8 34.59218667168386 37.0 35.0 37.0 31.0 37.0 9 36.13845228298582 39.0 37.0 39.0 32.0 39.0 10-11 36.073020776563446 39.0 37.0 39.0 31.0 39.0 12-13 36.105071160487526 39.0 37.0 39.0 31.5 39.0 14-15 37.42806062723708 40.0 38.0 41.0 31.0 41.0 16-17 37.435072309943706 40.0 38.0 41.0 31.0 41.0 18-19 37.443390998671546 40.0 38.0 41.0 31.0 41.0 20-21 37.47659770120201 40.0 38.0 41.0 31.0 41.0 22-23 37.45250887397328 40.0 38.0 41.0 31.0 41.0 24-25 37.46612478290433 40.0 38.0 41.0 31.0 41.0 26-27 37.42804575864966 40.0 38.0 41.0 31.0 41.0 28-29 37.44044330121528 40.0 38.0 41.0 31.0 41.0 30-31 37.341006191622924 40.0 38.0 41.0 30.5 41.0 32-33 37.3009627982224 40.0 37.5 41.0 30.0 41.0 34-35 37.29064314074891 40.0 37.0 41.0 30.0 41.0 36-37 37.22162001266118 40.0 37.0 41.0 30.0 41.0 38-39 37.12291003418059 40.0 37.0 41.0 30.0 41.0 40-41 37.056480044926005 40.0 37.0 41.0 30.0 41.0 42-43 36.919347063139455 40.0 37.0 41.0 30.0 41.0 44-45 36.793800714035314 40.0 36.5 41.0 30.0 41.0 46-47 36.67262601556742 40.0 36.0 41.0 30.0 41.0 48-49 36.51854541752995 40.0 36.0 41.0 30.0 41.0 50-51 36.39127030920371 40.0 36.0 41.0 29.5 41.0 52-53 36.22741647428049 39.5 35.0 41.0 29.0 41.0 54-55 36.04232457783219 39.0 35.0 41.0 29.0 41.0 56-57 35.84557685258311 39.0 35.0 41.0 29.0 41.0 58-59 35.61201479081328 39.0 35.0 41.0 28.0 41.0 60-61 35.35995191727575 38.0 35.0 40.0 28.0 41.0 62-63 35.08975051082176 38.0 34.0 40.0 28.0 41.0 64-65 34.7388381228294 37.0 34.0 40.0 28.0 41.0 66-67 34.42767461583287 37.0 34.0 40.0 27.0 41.0 68-69 34.09691488248383 36.0 34.0 39.0 26.5 41.0 70-71 33.73459171144876 36.0 33.5 39.0 26.0 41.0 72-73 33.37798322480218 35.0 33.0 38.5 26.0 40.0 74-75 33.02383777684881 35.0 33.0 37.0 26.0 39.5 76-77 32.7920613752414 35.0 33.0 37.0 26.0 39.0 78-79 32.62598297089245 35.0 33.0 36.5 26.0 39.0 80-81 32.37014690736241 35.0 33.0 36.0 26.0 38.5 82-83 32.08265533527806 35.0 33.0 36.0 25.0 37.0 84-85 31.834411401232835 35.0 33.0 35.0 25.0 37.0 86-87 31.636131925544976 35.0 33.0 35.0 25.0 36.5 88-89 31.373743675846494 35.0 32.0 35.0 24.0 36.0 90-91 31.25235767187658 35.0 32.0 35.0 24.0 36.0 92-93 31.10381533671918 35.0 32.0 35.0 23.0 36.0 94-95 31.003012890493427 35.0 32.0 35.0 23.0 35.0 96-97 30.89859966499929 35.0 32.0 35.0 21.0 35.0 98-99 30.761420933712976 35.0 32.0 35.0 20.0 35.0 100-101 30.668458236139475 35.0 32.0 35.0 19.5 35.0 102-103 29.641370815135993 33.5 30.0 34.5 18.0 35.0 104-105 30.34903208355231 34.5 31.5 35.0 18.0 35.0 106-107 30.675893673587613 35.0 32.0 35.0 19.0 35.0 108-109 30.713840882096104 35.0 32.0 35.0 19.0 35.0 110-111 30.635050235810077 35.0 32.0 35.0 18.0 35.0 112-113 30.538294332837907 35.0 32.0 35.0 16.0 35.0 114-115 30.428644505227737 35.0 32.0 35.0 9.0 35.0 116-117 30.296018421036077 35.0 32.0 35.0 3.5 35.0 118-119 30.110957691434493 35.0 32.0 35.0 2.0 35.0 120-121 29.924657436323848 35.0 32.0 35.0 2.0 35.0 122-123 29.741355489053575 35.0 32.0 35.0 2.0 35.0 124-125 28.93420392725143 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11022983004545495 1101 2 0.12120301274563161 1101 3 0.17581863417516175 1101 4 0.3242616229104911 1101 5 0.21628186972688468 1101 6 0.21573974119086614 1101 7 0.20935152125147027 1101 8 0.003244922775671455 1101 9 0.28208284712786025 1101 10-11 0.3513487661616068 1101 12-13 0.37092102780047753 1101 14-15 0.3890671290640597 1101 16-17 0.338513630002943 1101 18-19 0.14578559088835874 1101 20-21 0.3330951026958999 1101 22-23 0.4575884178620697 1101 24-25 0.41484646633298894 1101 26-27 0.5312543036592317 1101 28-29 0.4984177509547507 1101 30-31 0.5102935856190314 1101 32-33 0.4105365328844357 1101 34-35 0.4531472066326714 1101 36-37 0.34708116581354886 1101 38-39 0.5901836116224501 1101 40-41 0.43789543269393505 1101 42-43 0.5223436177073779 1101 44-45 0.40372380636700456 1101 46-47 0.44726772587380026 1101 48-49 0.31423431178743755 1101 50-51 0.3522438502552916 1101 52-53 0.2800119680603004 1101 54-55 0.32418054934004914 1101 56-57 0.28327737172203626 1101 58-59 0.4106463472445512 1101 60-61 0.2308987347960496 1101 62-63 0.17854164112318216 1101 64-65 0.23705307927929908 1101 66-67 0.2685584351547874 1101 68-69 0.25156670171469386 1101 70-71 0.31153792498003696 1101 72-73 0.3769779896922998 1101 74-75 0.42265709736685864 1101 76-77 0.44838674312956783 1101 78-79 0.4373196299441915 1101 80-81 0.4847557018685862 1101 82-83 0.3247356478818091 1101 84-85 0.27969952674713383 1101 86-87 0.241185108572175 1101 88-89 0.3986826580575702 1101 90-91 0.22690838869087315 1101 92-93 0.2501848169676535 1101 94-95 0.21958954600072644 1101 96-97 0.1757816625257469 1101 98-99 0.04011469202974638 1101 100-101 0.08546679018241221 1101 102-103 -0.03097723399896779 1101 104-105 0.10352782939279948 1101 106-107 0.11244837595936374 1101 108-109 0.16403994284880596 1101 110-111 0.15191378589829796 1101 112-113 0.16981252952473724 1101 114-115 0.16931199784603734 1101 116-117 0.13100351060185744 1101 118-119 0.3021562958336652 1101 120-121 0.06559284324908887 1101 122-123 -0.03084207995561883 1101 124-125 -0.04589240388024507 1102 1 0.30079751555313905 1102 2 0.2573358397038312 1102 3 0.3490239494274192 1102 4 0.3128714619930193 1102 5 0.41311693142612427 1102 6 0.4063050841044742 1102 7 0.3606573300414482 1102 8 0.3225245657268516 1102 9 0.5119901834765841 1102 10-11 0.45735653607763993 1102 12-13 0.42054044996685036 1102 14-15 0.5683856778176661 1102 16-17 0.640058898759392 1102 18-19 0.628704800440893 1102 20-21 0.6883063906260176 1102 22-23 0.6681281234261078 1102 24-25 0.6613098520560996 1102 26-27 0.6194199400530209 1102 28-29 0.5406413693630441 1102 30-31 0.6447669189336196 1102 32-33 0.6817662917003204 1102 34-35 0.5367649308868607 1102 36-37 0.379069053665134 1102 38-39 0.5057724507546482 1102 40-41 0.5904489070889127 1102 42-43 0.4366508236204609 1102 44-45 0.3732843731998017 1102 46-47 0.41450889031172267 1102 48-49 0.4582150631604307 1102 50-51 0.41407896349160467 1102 52-53 0.48187564220358325 1102 54-55 0.3900186873338356 1102 56-57 0.44225189647997354 1102 58-59 0.5390108979865431 1102 60-61 0.4100216769442895 1102 62-63 0.4916820477787738 1102 64-65 0.3814383966492585 1102 66-67 0.3922399060789772 1102 68-69 0.39791089625120435 1102 70-71 0.3277639028490782 1102 72-73 0.4531544638633278 1102 74-75 0.49328326339306017 1102 76-77 0.5021834790526896 1102 78-79 0.5418311850237316 1102 80-81 0.46908843268671774 1102 82-83 0.45115397146526703 1102 84-85 0.39143470492015453 1102 86-87 0.49522163242493633 1102 88-89 0.48312520968600126 1102 90-91 0.42979132551673516 1102 92-93 0.44198517547825134 1102 94-95 0.2877962808649954 1102 96-97 0.3669275002514425 1102 98-99 0.2759977516075409 1102 100-101 0.357730570023552 1102 102-103 0.1858710774123722 1102 104-105 0.28332824974788196 1102 106-107 0.3184120582548786 1102 108-109 0.38501098557491176 1102 110-111 0.44620849172939003 1102 112-113 0.413214379049613 1102 114-115 0.33547079011697534 1102 116-117 0.3528209325677061 1102 118-119 0.325618312544794 1102 120-121 0.1938318309485929 1102 122-123 0.2654370373174473 1102 124-125 0.16146676170648533 1103 1 0.27287450575489913 1103 2 0.2507003979215625 1103 3 0.2576777778296311 1103 4 0.28501245274616593 1103 5 0.2615568564470152 1103 6 0.20526735208709113 1103 7 0.2045458312346895 1103 8 0.2392190787872721 1103 9 0.3939120892653136 1103 10-11 0.41827866094517674 1103 12-13 0.4016570565099187 1103 14-15 0.3669338653097398 1103 16-17 0.3644156009094459 1103 18-19 0.4663044782227317 1103 20-21 0.34743299233212355 1103 22-23 0.4580134670536822 1103 24-25 0.44981256838719474 1103 26-27 0.4774307936849951 1103 28-29 0.38544818616023235 1103 30-31 0.5361988297316245 1103 32-33 0.5153888582947417 1103 34-35 0.5051388122618405 1103 36-37 0.4049143532554069 1103 38-39 0.33817308374471367 1103 40-41 0.3540341779774039 1103 42-43 0.3716494174544991 1103 44-45 0.24087338627438015 1103 46-47 0.23719367036520822 1103 48-49 0.271496839126506 1103 50-51 0.26631330581824386 1103 52-53 0.28863951586157555 1103 54-55 0.26821386870771136 1103 56-57 0.1853388369213036 1103 58-59 0.2528128168953785 1103 60-61 0.12202504189635732 1103 62-63 0.15351798051911913 1103 64-65 0.26302768418773326 1103 66-67 0.16039175477182255 1103 68-69 0.11126758055494435 1103 70-71 0.15264231718618504 1103 72-73 0.1748467584430955 1103 74-75 0.19620516548944522 1103 76-77 0.25079092859379415 1103 78-79 0.31830133615119394 1103 80-81 0.28676040032213734 1103 82-83 0.2907875129234725 1103 84-85 0.2146216431460779 1103 86-87 0.23376584142119938 1103 88-89 0.3432936203676924 1103 90-91 0.17176717946255238 1103 92-93 0.19168155747784965 1103 94-95 0.14260841463107 1103 96-97 0.14833130926887605 1103 98-99 0.10486696955772601 1103 100-101 0.013008138568753935 1103 102-103 -0.21037449024175103 1103 104-105 -0.020259058081236958 1103 106-107 -0.016827522270737916 1103 108-109 -0.01762502175068903 1103 110-111 -0.007906112950283983 1103 112-113 -0.13235224429360315 1103 114-115 -0.15214894937616563 1103 116-117 -0.16062372172470063 1103 118-119 -0.10454439347236288 1103 120-121 -0.24254054395186486 1103 122-123 -0.3149309627760921 1103 124-125 -0.3287606925608131 1104 1 0.39583527012251096 1104 2 0.3365198717593856 1104 3 0.3718281100545333 1104 4 0.40972196203515665 1104 5 0.35112813306744783 1104 6 0.35052278241633417 1104 7 0.3580916191651724 1104 8 0.36094201384557323 1104 9 0.5504236911864879 1104 10-11 0.554977514125099 1104 12-13 0.46601700609917174 1104 14-15 0.551276171747979 1104 16-17 0.5546413746252696 1104 18-19 0.5516566940119958 1104 20-21 0.5550240962029278 1104 22-23 0.5296978888138355 1104 24-25 0.5666607395932317 1104 26-27 0.5275129645466521 1104 28-29 0.5471812937361378 1104 30-31 0.523706126345381 1104 32-33 0.47885205710578305 1104 34-35 0.5510464194243667 1104 36-37 0.655589824299156 1104 38-39 0.4210999387026817 1104 40-41 0.400023681233705 1104 42-43 0.36807491628032096 1104 44-45 0.2625904290880996 1104 46-47 0.29957559650027576 1104 48-49 0.26381707681903777 1104 50-51 0.15545339530709157 1104 52-53 0.21656423678899728 1104 54-55 0.1956736976433291 1104 56-57 0.11246283844535299 1104 58-59 0.188792739911527 1104 60-61 0.10660537364498879 1104 62-63 0.15179938374037505 1104 64-65 0.251038492945618 1104 66-67 0.18121305428957868 1104 68-69 0.10318749670800287 1104 70-71 -0.1261282461368154 1104 72-73 -0.09221564012176486 1104 74-75 -0.17756723267242336 1104 76-77 0.05546935552934684 1104 78-79 0.14397660875146556 1104 80-81 0.144894995063936 1104 82-83 0.137160668467736 1104 84-85 0.0755376520998503 1104 86-87 0.205412238359429 1104 88-89 0.16080309680665295 1104 90-91 0.06480866758098003 1104 92-93 0.00583428628927507 1104 94-95 -0.05563096436909376 1104 96-97 -0.037992607870901196 1104 98-99 0.04853260818544669 1104 100-101 -0.030826726544638916 1104 102-103 -0.17130646626245039 1104 104-105 0.007707198591049291 1104 106-107 0.007883956188070584 1104 108-109 -0.023289895913105596 1104 110-111 -0.09473497713312895 1104 112-113 -0.05548218935224014 1104 114-115 -0.10614288372251579 1104 116-117 -0.11786197784011776 1104 118-119 -0.3290762347957461 1104 120-121 -0.3503789999761828 1104 122-123 -0.43982067400115454 1104 124-125 -0.40660563921310455 1105 1 0.026970381498284013 1105 2 0.006782934655440442 1105 3 -0.03350834908446387 1105 4 0.14062154494529722 1105 5 0.0798999295793621 1105 6 0.15175328420520628 1105 7 0.1612585523152248 1105 8 0.159483343400197 1105 9 0.09072214383398602 1105 10-11 0.15462036663554102 1105 12-13 0.1387227969281497 1105 14-15 0.19983100400190068 1105 16-17 0.18340691894290018 1105 18-19 0.13048943120079315 1105 20-21 0.21527811017724474 1105 22-23 0.21191503058167882 1105 24-25 0.16816547807152205 1105 26-27 0.12578776699407967 1105 28-29 0.11758275019135311 1105 30-31 0.19914131331586304 1105 32-33 0.22482678803918077 1105 34-35 0.06890546989823321 1105 36-37 0.006948423201357912 1105 38-39 0.09593992843684873 1105 40-41 0.15070861713267902 1105 42-43 0.17216656208083236 1105 44-45 0.15012555031582053 1105 46-47 0.1386717332417362 1105 48-49 0.10594283161847073 1105 50-51 0.17510831207493993 1105 52-53 0.1503408200916212 1105 54-55 0.1613929455553702 1105 56-57 0.13309303922531512 1105 58-59 0.11977667643586898 1105 60-61 0.0566813108438069 1105 62-63 0.07456030026833815 1105 64-65 0.06017379407330026 1105 66-67 0.01710060573355321 1105 68-69 -0.00738139321855158 1105 70-71 -0.001019943995224537 1105 72-73 -0.04081480611693422 1105 74-75 -0.02920506710469084 1105 76-77 0.03579534697943387 1105 78-79 0.1365210492463902 1105 80-81 0.17208555480438292 1105 82-83 0.19004993277975757 1105 84-85 0.11611905661667166 1105 86-87 0.12783423932101456 1105 88-89 0.20043705342108709 1105 90-91 0.13774641627512452 1105 92-93 0.09521158959983467 1105 94-95 5.573161623431133E-4 1105 96-97 -0.026528721434068814 1105 98-99 -0.05834158469489381 1105 100-101 -0.11695609508322136 1105 102-103 -0.17937806365145548 1105 104-105 -0.02981099507569951 1105 106-107 -0.11379576794588075 1105 108-109 -0.05766865734030091 1105 110-111 -0.057941105274547766 1105 112-113 -0.0744802613737292 1105 114-115 -0.14654211474634238 1105 116-117 -0.17315403765539727 1105 118-119 -0.05142459394451748 1105 120-121 -0.15921043571754367 1105 122-123 -0.2929236934029227 1105 124-125 -0.3662637564048765 1106 1 0.17993831834621332 1106 2 0.2344507476270934 1106 3 0.17673888124921433 1106 4 0.2589268113563392 1106 5 0.21070668344739119 1106 6 0.16232171964015407 1106 7 0.15167633296946548 1106 8 0.2943269913381741 1106 9 0.457440432749074 1106 10-11 0.372535384258164 1106 12-13 0.3774983822585227 1106 14-15 0.4135132968858173 1106 16-17 0.32136117118650276 1106 18-19 0.2975894936097774 1106 20-21 0.23726379463432323 1106 22-23 0.44799500140612736 1106 24-25 0.30562105768321146 1106 26-27 0.4619658885777369 1106 28-29 0.3233282293922102 1106 30-31 0.3701725426359346 1106 32-33 0.3503673020871929 1106 34-35 0.33838666202117196 1106 36-37 0.23008280969705197 1106 38-39 0.2978381375814365 1106 40-41 0.32635438748986445 1106 42-43 0.32080268173145043 1106 44-45 0.22275325973478033 1106 46-47 0.24722185005526853 1106 48-49 0.21657752844691203 1106 50-51 0.1434956775510443 1106 52-53 0.28066324084118577 1106 54-55 0.16340218961902053 1106 56-57 0.17141592397586436 1106 58-59 0.15279329914229578 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114-115 -0.9443206204445467 2216 116-117 -0.9886820411751636 2216 118-119 -1.0160912992355335 2216 120-121 -0.8821504889943874 2216 122-123 -0.9170308457658294 2216 124-125 -0.8437430372888173 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 27299.0 3 5276.0 4 3171.0 5 2664.0 6 3006.0 7 3596.0 8 3973.0 9 4337.0 10 4893.0 11 5606.0 12 6329.0 13 6545.0 14 6429.0 15 6256.0 16 5682.0 17 5055.0 18 4614.0 19 4599.0 20 4689.0 21 4928.0 22 5962.0 23 7762.0 24 11114.0 25 15880.0 26 19229.0 27 19822.0 28 22997.0 29 28496.0 30 33210.0 31 40965.0 32 52839.0 33 69905.0 34 97781.0 35 148426.0 36 289529.0 37 538044.0 38 226879.0 39 866.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.633815629129984 18.13140415014675 12.452442659435091 24.78233756128818 2 15.827196178673987 19.75993178164407 39.05089096215448 25.361981077527464 3 18.59675389178991 24.258714172400246 29.435433831422202 27.709098104387643 4 12.340526283281596 16.067641790635058 37.14905488160571 34.44277704447764 5 13.087800742912641 38.72669532001643 34.74226285196544 13.44324108510548 6 33.11240889441105 36.54388828156597 17.135373137487424 13.208329686535563 7 29.1294916673291 32.41537939802093 20.86516119476221 17.58996773988776 8 26.844979358373973 35.227498225775186 19.76763802496896 18.159884390881885 9 26.666125297586202 15.78517864893721 19.234119061929384 38.314576991547206 10-11 24.855681567014106 26.681746867084073 27.86048441355205 20.602087152349768 12-13 25.453268612263756 23.95987263186308 27.64592550061991 22.94093325525325 14-15 22.397494290584174 25.777622864312484 24.706851842267717 27.118031002835625 16-17 22.88098082464711 28.89856237751272 25.79940193898039 22.421054858859772 18-19 22.985833079328007 27.753222341107513 26.770636336238617 22.490308243325856 20-21 23.168653102118974 27.138855283628555 27.05453316455566 22.63795844969681 22-23 23.258680612643165 26.96116009327182 26.821566892221295 22.95859240186372 24-25 23.025122765923257 27.27542285404823 26.74375647998774 22.955697900040775 26-27 22.645751514380358 27.65037650419394 26.489825737246253 23.214046244179446 28-29 22.92019737562588 27.69177629199286 26.54551765923968 22.842508673141584 30-31 22.74946387212993 27.228118834529496 26.53032339233123 23.49209390100935 32-33 22.436403136931517 27.485894130419926 26.56366920452703 23.514033528121526 34-35 23.410584863595304 27.413584911918615 26.236898020946008 22.93893220354007 36-37 23.49200312812294 26.89782561143509 26.186048971994154 23.42412228844782 38-39 22.789780687959283 27.22159951962943 26.413433219912502 23.575186572498787 40-41 23.29193168184225 26.936645694201555 26.572534572542576 23.19888805141361 42-43 22.87745991732985 26.952722007324564 26.460138073332583 23.709680002013 44-45 22.830859341156433 27.20146606661407 26.708021075994303 23.25965351623519 46-47 23.409009742650266 27.56549832828325 26.006925908335653 23.018566020730834 48-49 22.968307605911523 27.361231759531478 26.17900749891488 23.49145313564212 50-51 23.078935615013386 27.432086297281394 26.379619055352894 23.10935903235233 52-53 23.32648321071833 27.07129951124609 26.38313239989809 23.21908487813749 54-55 22.670329428376416 27.348242683602987 26.596779861711394 23.384648026309204 56-57 22.817732068891964 27.62011973801519 26.52070081451359 23.041447378579257 58-59 22.804938234892123 26.945096220436326 26.565601515691327 23.68436402898022 60-61 22.36719867165074 27.32468983691007 26.865932399329424 23.442179092109765 62-63 22.61969948637212 27.372537335454222 27.04539729315276 22.962365885020898 64-65 22.51899131233421 27.26985749117094 26.68657179040602 23.52457940608883 66-67 22.39169372631685 27.457814968037397 26.626038112645155 23.524453193000596 68-69 22.76297157941933 26.91991613824151 27.10452180954734 23.212590472791824 70-71 22.65710066886878 26.92002397313545 26.76804733321743 23.65482802477834 72-73 22.552967846424128 27.169239760998465 26.612911527340604 23.664880865236807 74-75 22.658968000093786 27.460912625395707 26.580844434264954 23.29927494024555 76-77 22.920477972818798 27.14177058105322 26.731393343951137 23.206358102176843 78-79 22.921428185407784 27.06843519468744 26.84219841983106 23.167938200073714 80-81 23.059953758111345 27.29300807317267 26.579335491593138 23.06770267712285 82-83 22.801360025642733 27.368986302811294 26.657777871818926 23.171875799727047 84-85 22.801601813978557 27.413293454779925 26.754439515223954 23.030665216017567 86-87 23.065388663646942 27.328251783484458 26.586692828346575 23.01966672452203 88-89 22.813383775560684 27.445461486329986 26.491491448555504 23.249663289553826 90-91 22.854603749016025 27.496682432202523 26.578771969504395 23.069941849277054 92-93 22.917523883649718 26.90149193641778 26.968973201073865 23.212010978858636 94-95 22.562964094003583 26.86300260974914 27.162464676028826 23.41156862021845 96-97 22.97286903163731 26.550948661751438 27.147009331497628 23.329172975113625 98-99 22.66092506176228 26.822301903193342 26.78650265349071 23.730270381553666 100-101 22.542218765547887 26.680315446949898 27.22477024870899 23.552695538793227 102-103 22.409273792888303 26.43557672264489 27.406653164640932 23.748496319825875 104-105 22.269246121838826 26.86224936207494 27.031466775856412 23.837037740229825 106-107 22.604484643463266 26.725106482759013 26.702830532635414 23.967578341142307 108-109 22.57512283261167 26.67098245215726 26.741609595455156 24.012285119775914 110-111 22.590005487154368 26.85180022411837 26.9246000939593 23.63359419476796 112-113 22.545901445747923 27.10489271338586 27.278487690654952 23.070718150211267 114-115 22.544511246978303 27.349604226917144 26.76099148863407 23.344893037470484 116-117 22.36959867232522 27.231486983171312 27.020949651182274 23.377964693321193 118-119 22.296616143007206 27.347742455207264 26.71269713038125 23.64294427140428 120-121 22.27170466786946 27.621413821473705 26.85492683013318 23.251954680523657 122-123 22.280777856476806 27.67480024442768 26.911292598628194 23.133129300467324 124-125 22.20844300393313 27.671246977952457 26.934718054223143 23.185591963891266 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 576.0 1 508.5 2 419.5 3 340.5 4 281.0 5 249.5 6 213.5 7 209.0 8 222.0 9 208.5 10 175.5 11 163.5 12 155.0 13 175.0 14 221.5 15 261.0 16 301.0 17 358.0 18 455.0 19 584.0 20 772.5 21 1127.0 22 1604.5 23 2275.5 24 3237.0 25 4614.0 26 6644.0 27 9075.5 28 11442.5 29 14215.5 30 17473.5 31 21570.5 32 28651.0 33 32324.0 34 32487.5 35 35097.0 36 40189.0 37 45421.5 38 48109.0 39 50295.0 40 51674.0 41 54348.0 42 56495.5 43 58067.0 44 60496.5 45 63964.5 46 65726.0 47 65585.0 48 64093.0 49 61609.0 50 61778.0 51 61273.0 52 61382.0 53 63736.0 54 67328.5 55 64155.5 56 56825.0 57 52541.5 58 49808.0 59 44053.5 60 34147.5 61 27939.0 62 24074.5 63 18049.5 64 12850.5 65 9723.5 66 6912.5 67 5333.5 68 4051.5 69 2661.0 70 1789.0 71 1153.0 72 811.5 73 545.0 74 390.0 75 298.0 76 203.0 77 112.0 78 67.0 79 37.0 80 25.0 81 24.5 82 17.0 83 12.0 84 10.0 85 11.0 86 9.5 87 6.0 88 6.0 89 5.0 90 4.5 91 3.5 92 2.5 93 1.5 94 2.5 95 3.0 96 1.5 97 1.0 98 1.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04363358539401471 2 0.008578031204590046 3 0.0020587274891016114 4 0.005089631848056762 5 0.022817563004209528 6 0.031109659835313237 7 0.0030880912336524167 8 1.1437374939453397E-4 9 0.0 10-11 1.4296718674316745E-4 12-13 0.0012009243686426066 14-15 3.717146855322354E-4 16-17 1.1437374939453397E-4 18-19 1.1437374939453397E-4 20-21 8.578031204590048E-5 22-23 2.8593437348633493E-5 24-25 0.0 26-27 2.8593437348633493E-5 28-29 4.2890156022950236E-4 30-31 1.7156062409180096E-4 32-33 8.578031204590048E-5 34-35 8.292096831103712E-4 36-37 2.8593437348633493E-5 38-39 1.7156062409180096E-4 40-41 6.290556216699368E-4 42-43 9.721768698535386E-4 44-45 0.0016298259288721091 46-47 8.578031204590048E-5 48-49 0.0 50-51 0.0 52-53 0.0012867046806885071 54-55 0.003631366543276453 56-57 1.1437374939453397E-4 58-59 6.862424963672038E-4 60-61 9.435834325049053E-4 62-63 7.720228084131042E-4 64-65 0.001401078430083041 66-67 0.002687783110771548 68-69 0.0034883993565332856 70-71 0.0016584193662207425 72-73 0.0025734093613770143 74-75 0.0022016946758447787 76-77 0.0010293637445508057 78-79 0.002430442174633847 80-81 0.0012581112433398736 82-83 5.432753096240363E-4 84-85 6.004621843213033E-4 86-87 0.002258881550542046 88-89 0.006748051214277504 90-91 4.2890156022950236E-4 92-93 0.0011151440565967063 94-95 0.0010007703072021721 96-97 3.145278108349684E-4 98-99 7.434293710644708E-4 100-101 0.0013152981180371407 102-103 0.001515452179477575 104-105 8.578031204590047E-4 106-107 0.007462887147993341 108-109 0.001915760302358444 110-111 0.001343891555385774 112-113 0.002430442174633847 114-115 0.002859343734863349 116-117 0.0018299799903125436 118-119 0.04717917162524526 120-121 0.012666892745444636 122-123 0.004489169663735458 124-125 0.0026020027987256476 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1748653.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.6847155938502 #Duplication Level Percentage of deduplicated Percentage of total 1 84.75492841477677 59.061230817608525 2 9.40999980048975 13.114663196706308 3 2.737725918528651 5.72332956019744 4 1.092751651444777 3.0459235218255767 5 0.5602877723757463 1.9521747034357884 6 0.32487551742247467 1.3583314820994044 7 0.20510830669150823 1.0005039812413767 8 0.1472709996451421 0.8210030180394993 9 0.10383569734752252 0.651218493433609 >10 0.5816412555965205 7.663591648839057 >50 0.05939197708587285 2.8976557031577985 >100 0.02183335492433019 2.563340839682573 >500 3.493336709209721E-4 0.14703303373312096 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 2.859343734863349E-5 0.0 22-23 0.0 0.0 0.0 2.2874749878906792E-4 2.859343734863349E-5 24-25 0.0 0.0 0.0 5.146818722754029E-4 1.1437374939453396E-4 26-27 0.0 0.0 0.0 9.435834325049052E-4 1.1437374939453396E-4 28-29 0.0 0.0 0.0 0.0025734093613770143 1.1437374939453396E-4 30-31 0.0 0.0 0.0 0.011894869937031531 1.1437374939453396E-4 32-33 0.0 0.0 0.0 0.03122403358470777 1.1437374939453396E-4 34-35 0.0 0.0 0.0 0.060475119992359834 1.1437374939453396E-4 36-37 0.0 0.0 0.0 0.11005614035489031 1.1437374939453396E-4 38-39 0.0 0.0 0.0 0.18660077213718218 1.1437374939453396E-4 40-41 0.0 0.0 0.0 0.2696361141976138 1.1437374939453396E-4 42-43 0.0 0.0 0.0 0.34935461752560404 1.1437374939453396E-4 44-45 0.0 0.0 0.0 0.44620058982542565 1.1437374939453396E-4 46-47 0.0 0.0 0.0 0.5701531407317518 1.1437374939453396E-4 48-49 0.0 0.0 0.0 0.7031412178402462 1.1437374939453396E-4 50-51 0.0 0.0 0.0 0.8501114858122223 1.1437374939453396E-4 52-53 0.0 0.0 0.0 0.9848723560363319 1.7156062409180093E-4 54-55 0.0 0.0 0.0 1.1339585383721071 2.5734093613770143E-4 56-57 0.0 0.0 0.0 1.305833690274743 3.145278108349684E-4 58-59 0.0 0.0 0.0 1.4974097205105874 3.4312124818360187E-4 60-61 0.0 0.0 0.0 1.7155490540433123 3.4312124818360187E-4 62-63 0.0 0.0 0.0 1.926053939803952 3.4312124818360187E-4 64-65 0.0 0.0 0.0 2.1286670368563687 4.003081228808689E-4 66-67 0.0 0.0 0.0 2.37199718869324 4.003081228808689E-4 68-69 0.0 0.0 0.0 2.6442924925642766 4.003081228808689E-4 70-71 0.0 0.0 0.0 2.942979539108102 4.003081228808689E-4 72-73 0.0 0.0 0.0 3.25333270809017 4.003081228808689E-4 74-75 0.0 0.0 0.0 3.5532492724399867 4.003081228808689E-4 76-77 0.0 0.0 0.0 3.8715514170049747 4.003081228808689E-4 78-79 0.0 0.0 0.0 4.245582170962449 4.003081228808689E-4 80-81 0.0 0.0 0.0 4.646262008528851 4.003081228808689E-4 82-83 0.0 0.0 0.0 5.0592942110298615 4.003081228808689E-4 84-85 0.0 0.0 0.0 5.472240633218826 4.003081228808689E-4 86-87 0.0 0.0 0.0 5.882613646046414 4.003081228808689E-4 88-89 0.0 0.0 0.0 6.336362903331879 4.003081228808689E-4 90-91 0.0 0.0 0.0 6.8414945675328385 4.003081228808689E-4 92-93 0.0 0.0 0.0 7.350600719525257 4.003081228808689E-4 94-95 0.0 0.0 0.0 7.869657387715002 4.003081228808689E-4 96-97 0.0 0.0 0.0 8.393060258381738 4.2890156022950236E-4 98-99 0.0 0.0 0.0 8.927557382739742 4.5749499757813584E-4 100-101 0.0 0.0 0.0 9.534338716715094 4.5749499757813584E-4 102-103 0.0 0.0 0.0 10.156274572485222 4.860884349267694E-4 104-105 0.0 0.0 0.0 10.782728191356433 5.146818722754029E-4 106-107 0.0 0.0 0.0 11.400832526521842 5.146818722754029E-4 108-109 0.0 0.0 0.0 12.04138271000593 5.718687469726698E-4 110-111 0.0 0.0 0.0 12.721649177967269 5.718687469726698E-4 112-113 0.0 0.0 0.0 13.438000563862584 5.718687469726698E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1475 0.0 52.074055 1 GTATCAA 2245 0.0 45.087513 1 ATCAACG 2365 0.0 39.258236 3 TCAACGC 2425 0.0 38.04147 4 CAACGCA 2455 0.0 37.576603 5 GTACATG 6290 0.0 36.633972 1 AACGCAG 2585 0.0 35.681767 6 ACATGGG 6385 0.0 34.675232 3 TACATGG 6615 0.0 34.550243 2 TATCAAC 2755 0.0 34.133846 2 CATGGGA 3365 0.0 28.299074 4 ACGCAGA 3270 0.0 28.199081 7 GTGTAAG 1005 0.0 27.845531 1 CGCAGAG 3395 0.0 27.1616 8 CATGGGG 3735 0.0 26.13308 4 GAGTACA 4435 0.0 25.105663 1 TAAGGTG 1320 0.0 24.798538 5 GGACCGA 510 0.0 23.336464 6 AGGTAAG 1050 0.0 23.240437 2 TAAGGTA 800 0.0 23.06264 4 >>END_MODULE