FastQCFastQC Report
Thu 26 May 2016
SRR1364383_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364383_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1748653
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34490.1972375308308738No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34340.1963797277104148No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18360.10499510194418218No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28500.073.971641
GTATCAA48850.052.0559161
ATCAACG61100.040.8242383
TCAACGC61850.040.3291974
CAACGCA62500.040.0050245
TATCAAC62750.039.8456422
AACGCAG65100.038.573626
GTACATG62350.033.1436121
ACGCAGA77350.032.383117
CGCAGAG77650.032.2580038
TACATGG64950.030.7051282
ACATGGG62700.030.4777373
GCAGAGT90650.027.5662889
TAAGGTG11200.027.1079965
GAGTACT60000.026.12920612-13
CAGAGTA87000.025.88471810-11
TGGTATC12650.025.4125632
GTACTTT61900.025.08688414-15
CATGGGA32350.025.0270634
GTAAGGT12150.024.9884414
Produced by FastQC (version 0.11.5)