##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364383_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1748653 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.28337869205611 33.0 31.0 34.0 30.0 34.0 2 32.29965293285746 33.0 31.0 34.0 30.0 34.0 3 32.4235460094141 34.0 31.0 34.0 30.0 34.0 4 36.03143562502109 37.0 35.0 37.0 35.0 37.0 5 35.963812717560316 37.0 35.0 37.0 35.0 37.0 6 36.019208499342064 37.0 35.0 37.0 35.0 37.0 7 35.976352083575186 37.0 35.0 37.0 35.0 37.0 8 35.975662981735084 37.0 35.0 37.0 35.0 37.0 9 37.723934365480176 39.0 38.0 39.0 35.0 39.0 10-11 37.68905208752108 39.0 37.5 39.0 35.0 39.0 12-13 37.657410017882334 39.0 37.0 39.0 35.0 39.0 14-15 39.06859622806812 41.0 38.0 41.0 35.5 41.0 16-17 39.07393090567425 41.0 38.0 41.0 35.5 41.0 18-19 39.13061424993981 41.0 38.5 41.0 36.0 41.0 20-21 39.13091505290072 41.0 39.0 41.0 35.0 41.0 22-23 39.10177691057059 41.0 39.0 41.0 35.0 41.0 24-25 39.064700658163744 41.0 39.0 41.0 35.0 41.0 26-27 38.96954856109245 41.0 38.5 41.0 35.0 41.0 28-29 38.880896324199256 40.5 38.0 41.0 35.0 41.0 30-31 38.76277712044642 40.0 38.0 41.0 35.0 41.0 32-33 38.632551169385806 40.0 38.0 41.0 34.0 41.0 34-35 38.531099366197864 40.0 38.0 41.0 34.0 41.0 36-37 38.40707047081382 40.0 38.0 41.0 34.0 41.0 38-39 38.31154351377889 40.0 38.0 41.0 34.0 41.0 40-41 38.19536580442203 40.0 38.0 41.0 33.0 41.0 42-43 38.03956845640616 40.0 38.0 41.0 33.0 41.0 44-45 37.87511701864235 40.0 37.5 41.0 33.0 41.0 46-47 37.64409205256847 40.0 37.0 41.0 33.0 41.0 48-49 37.41780273158825 40.0 37.0 41.0 32.0 41.0 50-51 37.25330468652157 40.0 37.0 41.0 32.0 41.0 52-53 37.09002786716404 40.0 36.0 41.0 31.0 41.0 54-55 36.9014552915873 40.0 36.0 41.0 31.0 41.0 56-57 36.69392612485153 39.0 35.5 41.0 31.0 41.0 58-59 36.46711154242723 39.0 35.0 41.0 31.0 41.0 60-61 36.20313292574342 39.0 35.0 41.0 30.0 41.0 62-63 35.95371837637313 38.0 35.0 41.0 30.0 41.0 64-65 35.64761447811544 37.5 35.0 40.0 30.0 41.0 66-67 35.31868472475671 37.0 35.0 40.0 29.0 41.0 68-69 34.97870475160023 36.5 34.0 39.5 29.0 41.0 70-71 34.71434698593717 36.0 34.0 39.0 29.0 41.0 72-73 34.522579093736724 36.0 34.0 39.0 29.0 41.0 74-75 34.19613125073985 35.0 34.0 37.5 29.0 40.0 76-77 33.86772561508773 35.0 34.0 37.0 29.0 39.0 78-79 33.553472301251304 35.0 34.0 37.0 29.0 39.0 80-81 33.27147581595662 35.0 34.0 36.0 29.0 39.0 82-83 33.00545877312423 35.0 34.0 36.0 29.0 37.0 84-85 32.77917945984709 35.0 34.0 35.5 28.0 37.0 86-87 32.606039620210524 35.0 34.0 35.0 28.0 36.5 88-89 32.449266092243576 35.0 33.5 35.0 27.0 36.0 90-91 32.30936783913103 35.0 33.0 35.0 27.0 36.0 92-93 32.18239725091256 35.0 33.0 35.0 27.0 36.0 94-95 32.10163394338385 35.0 33.0 35.0 27.0 35.5 96-97 32.02556710793965 35.0 33.0 35.0 27.0 35.0 98-99 31.95310762055136 35.0 33.0 35.0 26.0 35.0 100-101 31.892836085832926 35.0 33.0 35.0 26.0 35.0 102-103 30.90827940134492 34.0 31.5 35.0 24.5 35.0 104-105 31.632019331451126 35.0 33.0 35.0 25.0 35.0 106-107 31.92870054836494 35.0 33.0 35.0 27.0 35.0 108-109 31.952739623012683 35.0 33.0 35.0 27.0 35.0 110-111 31.91386455746223 35.0 33.0 35.0 27.0 35.0 112-113 31.847398254542213 35.0 33.0 35.0 26.0 35.0 114-115 31.766006177326204 35.0 33.0 35.0 25.0 35.0 116-117 31.708246576078842 35.0 33.0 35.0 25.0 35.0 118-119 31.602960392942453 35.0 33.0 35.0 25.0 35.0 120-121 31.46874537143733 35.0 33.0 35.0 25.0 35.0 122-123 31.328551176248233 35.0 33.0 35.0 24.5 35.0 124-125 30.642578887863973 34.5 32.0 35.0 21.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.014285541373737942 1101 2 0.02581443335267153 1101 3 0.05635545625456473 1101 4 0.03413102508928745 1101 5 0.13017920334399236 1101 6 0.14885605477434183 1101 7 0.1623796835789051 1101 8 0.16095862606638178 1101 9 0.14044329300968172 1101 10-11 0.21229366553600215 1101 12-13 0.15858278346696864 1101 14-15 0.1990755318607853 1101 16-17 0.20436108580591394 1101 18-19 0.2639969918213154 1101 20-21 0.25384584431139245 1101 22-23 0.25687766090106834 1101 24-25 0.22498280687240424 1101 26-27 0.15612589991960135 1101 28-29 0.08204609525820672 1101 30-31 0.1802430585330015 1101 32-33 0.040641826837955364 1101 34-35 0.10867726928441357 1101 36-37 0.2038864140046499 1101 38-39 0.2644463079945609 1101 40-41 0.1764067281839985 1101 42-43 0.1952210292393417 1101 44-45 0.11777550589457775 1101 46-47 0.17266671368862774 1101 48-49 0.07342321947272978 1101 50-51 0.08806020355562083 1101 52-53 0.0864734202688382 1101 54-55 0.1232789102569285 1101 56-57 0.22240012797297481 1101 58-59 -0.02794612190869117 1101 60-61 0.11943056562331122 1101 62-63 -0.10916603477232201 1101 64-65 -0.26977085090477004 1101 66-67 -0.14440844111341278 1101 68-69 -0.1850681760385413 1101 70-71 -0.04125716525396683 1101 72-73 -0.06908565876042871 1101 74-75 -0.12107076102755343 1101 76-77 -0.21607957505467823 1101 78-79 -0.32097327300020595 1101 80-81 -0.3681673610883607 1101 82-83 -0.3016858156193578 1101 84-85 -0.4249616222736634 1101 86-87 -0.4291509524979844 1101 88-89 -0.5112848170530739 1101 90-91 -0.7272363366611501 1101 92-93 -0.669324539244414 1101 94-95 -0.4566905096483822 1101 96-97 -0.412152431336672 1101 98-99 -0.28043037757581146 1101 100-101 -0.4139737180539278 1101 102-103 -0.18036871148797928 1101 104-105 -0.2409073601922529 1101 106-107 -0.1432163028548068 1101 108-109 -0.14134256460733496 1101 110-111 -0.12491249285096373 1101 112-113 -0.2964389447324578 1101 114-115 -0.426088963095971 1101 116-117 -0.3035581134746934 1101 118-119 -0.23303081329721564 1101 120-121 -0.23618572960423023 1101 122-123 -0.18852031952218695 1101 124-125 -0.15338458651576303 1102 1 0.15713237459834772 1102 2 0.2129544352080046 1102 3 0.17567501700880683 1102 4 0.13208659638707587 1102 5 0.07462692742014099 1102 6 0.13053228391002847 1102 7 0.19476464112641168 1102 8 0.23635642196646955 1102 9 0.30097939097884563 1102 10-11 0.2610250102880727 1102 12-13 0.2955990026513149 1102 14-15 0.34536842358701847 1102 16-17 0.3751621967577279 1102 18-19 0.3579284550149495 1102 20-21 0.3697789510690299 1102 22-23 0.31359444034021067 1102 24-25 0.28956508018191585 1102 26-27 0.3347690788010169 1102 28-29 0.34968442309581604 1102 30-31 0.3984109531874793 1102 32-33 0.40796854281153827 1102 34-35 0.3015922340564785 1102 36-37 0.31494200563615493 1102 38-39 0.35163367710212157 1102 40-41 0.32605046647688596 1102 42-43 0.2179797992087842 1102 44-45 0.11803155142711574 1102 46-47 0.20415609752079433 1102 48-49 0.16513577755735298 1102 50-51 0.14806692211543293 1102 52-53 0.18751395370562562 1102 54-55 0.1438279112342542 1102 56-57 0.1328652453416197 1102 58-59 0.12328542664906195 1102 60-61 0.11262931224177919 1102 62-63 0.15127771813835267 1102 64-65 0.20911722276137823 1102 66-67 0.1383213462821189 1102 68-69 0.07088224717450231 1102 70-71 0.03344441168666634 1102 72-73 0.0893196156369882 1102 74-75 0.12933521052585206 1102 76-77 0.11213940689141566 1102 78-79 0.11271772759668153 1102 80-81 0.0584436174319265 1102 82-83 0.11383251143113426 1102 84-85 0.13902844325338037 1102 86-87 0.11516132278868696 1102 88-89 0.08222146531571894 1102 90-91 0.015379104032334112 1102 92-93 0.08579051492105094 1102 94-95 0.06682491820767922 1102 96-97 0.01524519027441329 1102 98-99 0.1289627687435484 1102 100-101 0.1710118468813775 1102 102-103 0.21000186815413002 1102 104-105 0.024651681143286197 1102 106-107 0.14706717913954392 1102 108-109 0.19767811862428175 1102 110-111 0.14751198894380835 1102 112-113 0.10054641780271822 1102 114-115 -0.010878687166496093 1102 116-117 -0.14005582288620388 1102 118-119 -0.14269874590172194 1102 120-121 -0.04434788958840841 1102 122-123 -0.05804204614520714 1102 124-125 -0.09630189388190757 1103 1 0.08335450820442247 1103 2 0.13785409588200537 1103 3 0.18114721800007771 1103 4 0.11372074585052161 1103 5 -0.02382576694153471 1103 6 0.004447813028427561 1103 7 0.05963686156400172 1103 8 0.19829655560454285 1103 9 0.25504914911829957 1103 10-11 0.23679033995860976 1103 12-13 0.19984871618731148 1103 14-15 0.35686744573244766 1103 16-17 0.29802091677131415 1103 18-19 0.2991298715194617 1103 20-21 0.20281874341185357 1103 22-23 0.2578797295673141 1103 24-25 0.3160778470611447 1103 26-27 0.24755340432751893 1103 28-29 0.3106326251110971 1103 30-31 0.26485403331247426 1103 32-33 0.24170318729463247 1103 34-35 0.2131344719588384 1103 36-37 0.21965095389786882 1103 38-39 0.18024635438056436 1103 40-41 0.13514282173409953 1103 42-43 0.20570277036053142 1103 44-45 0.22949158862127916 1103 46-47 0.15584368129074733 1103 48-49 0.04133301864126793 1103 50-51 0.02127729131245104 1103 52-53 0.10995243932949705 1103 54-55 0.08356977670263888 1103 56-57 -0.03334964820207631 1103 58-59 -0.048540822930931427 1103 60-61 -0.03357202426204253 1103 62-63 -0.046044904948864485 1103 64-65 0.04362756623510933 1103 66-67 -0.08828745219368273 1103 68-69 0.03686610795853795 1103 70-71 0.10796705372789006 1103 72-73 0.12198151241641142 1103 74-75 0.11121636211731811 1103 76-77 0.10901307226453127 1103 78-79 0.12060810326761384 1103 80-81 0.15634069162211262 1103 82-83 0.2082334016541907 1103 84-85 0.13289962122045296 1103 86-87 0.14566627790829756 1103 88-89 0.1656007990453645 1103 90-91 0.20535697153396626 1103 92-93 0.12737164238922816 1103 94-95 0.10605248693750724 1103 96-97 0.07902627823455077 1103 98-99 0.20599174007007548 1103 100-101 0.1799147697373229 1103 102-103 0.216645002310365 1103 104-105 0.1430920861683127 1103 106-107 0.12574538005943126 1103 108-109 0.11978306291303653 1103 110-111 0.07646106912049433 1103 112-113 0.17374635614064715 1103 114-115 0.23214156039256295 1103 116-117 0.15446925302214254 1103 118-119 0.08522675622568343 1103 120-121 0.09842072503349897 1103 122-123 -0.03313452386501936 1103 124-125 -0.17070545797415448 1104 1 0.11384275241323394 1104 2 0.06623676327976113 1104 3 0.09692948826665315 1104 4 0.04385963796199377 1104 5 0.2024005526464876 1104 6 0.2356860514365806 1104 7 0.1903297467855225 1104 8 0.17499486535465536 1104 9 0.19040054060391043 1104 10-11 0.18977938710022357 1104 12-13 0.19415025928373808 1104 14-15 0.20741829427672087 1104 16-17 0.22071802609107039 1104 18-19 0.2629865226729393 1104 20-21 0.16182032241128042 1104 22-23 0.21358050673300966 1104 24-25 0.24423680760813937 1104 26-27 0.1581825556122709 1104 28-29 0.2181531594308268 1104 30-31 0.29134081758793684 1104 32-33 0.1885541489211633 1104 34-35 0.12298809089608653 1104 36-37 0.1596124161758965 1104 38-39 0.15119667268389492 1104 40-41 0.01312283206537046 1104 42-43 0.025021516979364833 1104 44-45 0.06983679477657034 1104 46-47 0.11371077423434883 1104 48-49 0.10581417139633942 1104 50-51 0.0034356205039216547 1104 52-53 0.055356595407424436 1104 54-55 0.13521278178400564 1104 56-57 0.0992767633415923 1104 58-59 0.14315205862701674 1104 60-61 0.13038418445407984 1104 62-63 0.08884322788735943 1104 64-65 0.11121367238343538 1104 66-67 -0.011356337722943977 1104 68-69 -0.020366973361767293 1104 70-71 -0.037366990745432815 1104 72-73 -0.018038139593123503 1104 74-75 0.04092180789461963 1104 76-77 0.05724512412116667 1104 78-79 0.08059655691060641 1104 80-81 0.1210978181850848 1104 82-83 0.11427827979768068 1104 84-85 0.08758200176053776 1104 86-87 0.06350358296048597 1104 88-89 -5.069185127908327E-4 1104 90-91 0.024258757891551852 1104 92-93 0.010589949745799743 1104 94-95 0.06660482533430212 1104 96-97 -0.025823237502322627 1104 98-99 0.047860992078625486 1104 100-101 0.05429234415973383 1104 102-103 0.04378181107069423 1104 104-105 -0.0029916166769368147 1104 106-107 0.032671805158319245 1104 108-109 0.054065836310130067 1104 110-111 0.163566609295831 1104 112-113 0.14523865409161374 1104 114-115 0.1183580432903959 1104 116-117 0.12679730328146022 1104 118-119 0.07396020451375662 1104 120-121 -0.05335372594974075 1104 122-123 -0.20246900857754113 1104 124-125 -0.32712058583891235 1105 1 0.0727761834498537 1105 2 0.08503342428738847 1105 3 0.12274551256771815 1105 4 0.12463609345169147 1105 5 0.06215698522895963 1105 6 0.14087917647128734 1105 7 0.1662118709167899 1105 8 0.1834401370124965 1105 9 0.1422093849375372 1105 10-11 0.1616455330130364 1105 12-13 0.2004477783285239 1105 14-15 0.18644837636701084 1105 16-17 0.214074814625981 1105 18-19 0.18027771650830005 1105 20-21 0.15856393478923536 1105 22-23 0.1904985422148684 1105 24-25 0.18449461370587272 1105 26-27 0.1811308776559457 1105 28-29 0.10567516416703882 1105 30-31 0.10825048183344421 1105 32-33 0.09210938851500572 1105 34-35 0.1304534149003871 1105 36-37 0.07184224742057665 1105 38-39 -0.009828080904597414 1105 40-41 -0.07759599684762719 1105 42-43 -0.03418654424142886 1105 44-45 0.0030444866952521465 1105 46-47 -0.1064278204443525 1105 48-49 -0.050616166698716825 1105 50-51 -0.03210212868573592 1105 52-53 -0.18012989744823926 1105 54-55 -0.27406387140035804 1105 56-57 -0.17403572351459218 1105 58-59 -0.2817332194374984 1105 60-61 -0.3072982714078307 1105 62-63 -0.2177072499828583 1105 64-65 -0.3337582007579414 1105 66-67 -0.3164495005185657 1105 68-69 -0.30427357752980555 1105 70-71 -0.3031508300911412 1105 72-73 -0.27929256294770255 1105 74-75 -0.2246978566992084 1105 76-77 -0.1871332569277797 1105 78-79 -0.13784971888752295 1105 80-81 -0.09662559319283304 1105 82-83 -0.11880534468532744 1105 84-85 -0.12544854756833246 1105 86-87 -0.2028387274453678 1105 88-89 -0.16018096495479028 1105 90-91 -0.20169809380654868 1105 92-93 -0.09879648847239508 1105 94-95 -0.26780260291375413 1105 96-97 -0.2261737101550878 1105 98-99 -0.24258083110786188 1105 100-101 -0.2586737011787328 1105 102-103 -0.21116438143771887 1105 104-105 -0.17873409389541095 1105 106-107 -0.1935289404122038 1105 108-109 -0.20425311624767062 1105 110-111 -0.23543341854027844 1105 112-113 -0.1805170634963318 1105 114-115 -0.21479588558703 1105 116-117 -0.2494365031345751 1105 118-119 -0.38561673616953485 1105 120-121 -0.292119414548786 1105 122-123 -0.3136302538339244 1105 124-125 -0.4919333821714247 1106 1 0.053693501387257925 1106 2 0.12377022872468046 1106 3 0.07288167373871346 1106 4 0.013076390994456233 1106 5 0.054501397227582515 1106 6 -0.04510217274423667 1106 7 0.045759529894908724 1106 8 0.07276691611806996 1106 9 0.12012139768300756 1106 10-11 0.0029383664238409324 1106 12-13 6.634702935173209E-4 1106 14-15 0.0437599444591541 1106 16-17 0.013999649892788568 1106 18-19 -0.06514926283680467 1106 20-21 0.055615431192372 1106 22-23 -0.04100476957067656 1106 24-25 -0.0012782775081561226 1106 26-27 0.04391628747924159 1106 28-29 -0.06427546510217041 1106 30-31 -0.015227068481124206 1106 32-33 0.019662742206172368 1106 34-35 -0.01635709206775715 1106 36-37 0.036245796428751476 1106 38-39 0.03919785948925636 1106 40-41 -0.03014504868476564 1106 42-43 0.013950560341136509 1106 44-45 -0.08098056764508499 1106 46-47 -0.06868878931605593 1106 48-49 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-0.5877821249264699 2216 102-103 -0.6529824320321502 2216 104-105 -0.5753841248567895 2216 106-107 -0.5868034260831898 2216 108-109 -0.43563667510015947 2216 110-111 -0.4342500897997752 2216 112-113 -0.32000518688033 2216 114-115 -0.38254353884832426 2216 116-117 -0.45254443863091964 2216 118-119 -0.37805323439689786 2216 120-121 -0.3092972843812589 2216 122-123 -0.3646007153676649 2216 124-125 -0.38415764455158197 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 232.0 3 15.0 4 34.0 5 103.0 6 248.0 7 606.0 8 1115.0 9 1986.0 10 2996.0 11 4349.0 12 5752.0 13 7917.0 14 11494.0 15 10057.0 16 5429.0 17 4645.0 18 4583.0 19 4381.0 20 4162.0 21 4326.0 22 4661.0 23 5132.0 24 6401.0 25 8739.0 26 11465.0 27 15016.0 28 19487.0 29 25040.0 30 31584.0 31 39361.0 32 50715.0 33 65567.0 34 90138.0 35 136882.0 36 277732.0 37 595912.0 38 288681.0 39 1710.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.994879327860296 18.389688183557105 12.821243238790808 24.79418924979179 2 16.251537612093422 20.22030671608375 38.03561941677394 25.492536255048886 3 18.73950978267272 24.390945487755435 30.040322465349046 26.829222264222807 4 12.695371809043877 16.48789096521723 36.78276936590621 34.03396785983268 5 12.795888543927969 38.34555318927369 34.98273733165036 13.875820935147983 6 32.771795643216414 36.946465075150684 16.6331875581261 13.648551723506797 7 28.361258637362585 32.73805609231791 21.071704906576663 17.828980363742836 8 26.925181839964818 34.72049629057337 19.60451844934358 18.749803420118226 9 26.615800847852604 14.887916584937091 19.51267632857977 38.983606238630536 10-11 24.968361361573738 26.137404047572616 27.900332427302615 20.99390216355103 12-13 25.92112328746755 23.487936142848238 27.990601909012252 22.600338660671955 14-15 22.560193474634477 25.108097489896508 25.641336503011175 26.69037253245784 16-17 22.256016488119712 28.4394045016364 26.74541489935396 22.559164110889927 18-19 22.395237934570208 26.519812678673244 28.813292288407133 22.271657098349415 20-21 22.560055036868526 25.93932677054132 29.34170209407736 22.158916098512794 22-23 22.576320173299106 25.83208332356391 29.09433718410685 22.49725931903013 24-25 22.479159673188448 26.171029929894612 28.953485911727483 22.396324485189457 26-27 21.960727911559026 26.54044142140793 28.82700991881435 22.671820748218693 28-29 22.247673631285426 26.591343712399524 28.876394791862054 22.284587864452995 30-31 22.19786057877203 26.098125382973898 28.83592232981948 22.86809170843459 32-33 21.910467529103155 26.24758349367126 28.94820891882054 22.893740058405037 34-35 22.775296814855352 26.249464586660903 28.54626628900368 22.42897230948006 36-37 22.92358746989826 25.763859382050068 28.407580005867374 22.9049731421843 38-39 22.430522236258422 26.167827464911564 28.465767650871843 22.935882647958174 40-41 22.73486169199523 25.892609247754628 28.64195638753634 22.7305726727138 42-43 22.474670503524713 25.928471800866156 28.42038986579956 23.176467829809575 44-45 22.48532012087025 26.113486273998486 28.604090465112552 22.797103140018713 46-47 23.182872759775666 26.54557536572436 27.63092506060379 22.64062681389618 48-49 22.87944501059677 26.58073732476442 27.526612018670445 23.013205645968366 50-51 23.000046321368504 26.753135127438092 27.534994078299125 22.71182447289428 52-53 23.229851777339473 26.428742580717845 27.482868242012565 22.858537399930118 54-55 22.633020959561446 26.62926835684381 27.63192583091099 23.105784852683755 56-57 22.791073911610074 26.811576590963483 27.534791849367185 22.86255764805926 58-59 22.710192028026174 26.220440785496933 27.66372477984458 23.405642406632314 60-61 22.286201681695868 26.632207732644268 27.925146891762026 23.15644369389784 62-63 22.49777400090241 26.583318703024556 28.114182745233045 22.80472455083999 64-65 22.23828855696356 26.403923477099227 28.02765900381608 23.33012896212113 66-67 22.169349779516004 26.646738947063824 27.837569832322362 23.346341441097803 68-69 22.48136779606011 26.208952321859257 28.179183685723718 23.13049619635691 70-71 22.419383616637273 26.219053305060125 27.81737018000151 23.54419289830109 72-73 22.337206030702504 26.508419056507528 27.545160500774312 23.609214412015657 74-75 22.373764263121384 26.812180575563023 27.393313596236645 23.42074156507895 76-77 22.607973108444043 26.51789691837088 27.592066579247 23.282063393938078 78-79 22.589730495415612 26.476179093279228 27.549262203536095 23.384828207769065 80-81 22.80985983754892 26.757849414648277 27.108863154108352 23.323427593694447 82-83 22.58338275232422 26.737837638456575 27.214433052183594 23.464346557035615 84-85 22.47355535946812 26.72168234635459 27.357142897990627 23.447619396186663 86-87 22.73604311432857 26.627095255605315 27.177404550817112 23.459457079249 88-89 22.455312746439688 26.87745939302995 26.947770655470237 23.719457205060124 90-91 22.44607706617608 26.90508065350873 26.98411291434035 23.66472936597484 92-93 22.415882396335924 26.34630770084173 27.33529751185627 23.902512390966073 94-95 22.08136777279426 26.2498048497901 27.512433856231056 24.15639352118459 96-97 22.489167376260472 25.920093923723 27.52069735962481 24.070041340391718 98-99 22.276756354902354 26.108483687416005 27.09049838446802 24.52426157321362 100-101 22.104356896422562 25.938164976127336 27.47769282985246 24.479785297597637 102-103 21.890712991860877 25.651894816151295 27.65540895060625 24.801983241381578 104-105 21.773321205733524 25.992027466097 27.384575969109505 24.85007535905997 106-107 22.08210155082359 25.855151812696814 27.00066308238091 25.062083554098685 108-109 22.02653223742621 25.809985259868096 27.082754903488592 25.080727599217102 110-111 22.04062480162554 25.933682453318024 27.20021937713136 24.825473367925074 112-113 21.877210630125017 26.073812242909256 27.47820751172474 24.57076961524099 114-115 21.776847665031312 26.265445460019798 27.063774230793648 24.893932644155246 116-117 21.68085949585195 26.10417847337351 27.277824702785512 24.937137327989028 118-119 21.45385619674115 26.32540589813988 26.940536515821034 25.280201389297936 120-121 21.362356932163515 26.48486314554232 27.121555763716614 25.031224158577555 122-123 21.2348514373603 26.591187034262926 27.131893108899806 25.04206841947697 124-125 21.09222449413162 26.468881878407448 27.1081744990354 25.33071912842553 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 64.0 1 55.0 2 131.0 3 217.0 4 153.5 5 119.5 6 260.5 7 637.5 8 1122.0 9 1372.5 10 1094.0 11 741.5 12 722.5 13 812.0 14 925.0 15 1021.0 16 1150.5 17 1315.5 18 1500.0 19 1693.5 20 1916.0 21 2213.5 22 2663.5 23 3354.5 24 4308.5 25 5760.0 26 7673.0 27 10077.5 28 12577.0 29 15346.5 30 18659.5 31 22529.0 32 29356.0 33 32788.5 34 32483.5 35 35054.5 36 39914.5 37 44707.0 38 47536.0 39 50036.5 40 51543.0 41 53040.5 42 54584.5 43 56263.0 44 58969.5 45 62584.0 46 64304.5 47 64733.0 48 63166.5 49 60795.0 50 61097.5 51 60759.5 52 61184.0 53 63424.0 54 66798.5 55 63510.5 56 56079.0 57 51648.0 58 48690.5 59 43324.5 60 34397.5 61 28344.0 62 23832.0 63 17502.0 64 12462.5 65 9271.5 66 6408.5 67 4952.5 68 3734.0 69 2413.0 70 1605.5 71 1058.0 72 715.5 73 496.5 74 367.0 75 264.0 76 156.5 77 60.5 78 34.5 79 15.5 80 5.0 81 4.5 82 5.0 83 3.5 84 2.0 85 4.5 86 3.5 87 1.5 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025448159240283808 2 0.0 3 0.0 4 0.0 5 0.0543847178371009 6 0.01709887553448283 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 4.0030812288086883E-4 22-23 0.0 24-25 0.0 26-27 3.717146855322354E-4 28-29 2.859343734863349E-4 30-31 1.4296718674316745E-4 32-33 1.4296718674316745E-4 34-35 2.2874749878906795E-4 36-37 0.0 38-39 0.0 40-41 8.578031204590048E-5 42-43 0.0 44-45 5.718687469726699E-5 46-47 0.0 48-49 4.0030812288086883E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 2.0015406144043441E-4 58-59 1.1437374939453397E-4 60-61 8.578031204590048E-5 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.8593437348633493E-5 70-71 5.718687469726699E-5 72-73 5.718687469726699E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.0015406144043441E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 1.7156062409180096E-4 100-101 0.0 102-103 2.8593437348633493E-5 104-105 0.001401078430083041 106-107 8.578031204590048E-5 108-109 0.001458265304780308 110-111 0.003774333730019621 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 4.0030812288086883E-4 122-123 7.720228084131042E-4 124-125 8.578031204590048E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1748653.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.09333094153057 #Duplication Level Percentage of deduplicated Percentage of total 1 83.40988364956614 55.128370438453345 2 10.244514891012688 13.541882260542792 3 2.90247694688931 5.7550310820275525 4 1.2316011386598773 3.256024865816526 5 0.5792591165019777 1.9142582243931912 6 0.3533610597687808 1.4012885679088796 7 0.22152624213128755 1.0248985063391773 8 0.1659282697897864 0.8773401638217536 9 0.11796870966880853 0.7017250471997308 >10 0.6765364141447567 8.567760583838933 >50 0.06469134816732389 2.967149375476199 >100 0.03115891833451643 3.5419214419388863 >500 3.644317882688908E-4 0.14716858091266125 >1k 7.288635765377816E-4 1.1751808613303996 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3449 0.1972375308308738 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3434 0.1963797277104148 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1836 0.10499510194418218 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 2.859343734863349E-5 5.718687469726698E-5 22-23 0.0 0.0 0.0 2.2874749878906792E-4 1.1437374939453396E-4 24-25 0.0 0.0 0.0 5.146818722754029E-4 1.7156062409180093E-4 26-27 0.0 0.0 0.0 9.721768698535386E-4 2.0015406144043441E-4 28-29 0.0 0.0 0.0 0.0026305962360742815 2.2874749878906792E-4 30-31 0.0 0.0 0.0 0.011952056811728798 2.5734093613770143E-4 32-33 0.0 0.0 0.0 0.03130981389675367 2.859343734863349E-4 34-35 0.0 0.0 0.0 0.06067527405380027 2.859343734863349E-4 36-37 0.0 0.0 0.0 0.11022770097898212 2.859343734863349E-4 38-39 0.0 0.0 0.0 0.18711545400945756 2.859343734863349E-4 40-41 0.0 0.0 0.0 0.2702365763819351 2.859343734863349E-4 42-43 0.0 0.0 0.0 0.34995507970992534 3.145278108349684E-4 44-45 0.0 0.0 0.0 0.44645793076156337 3.4312124818360187E-4 46-47 0.0 0.0 0.0 0.5705820422919813 3.4312124818360187E-4 48-49 0.0 0.0 0.0 0.703856053773962 3.4312124818360187E-4 50-51 0.0 0.0 0.0 0.8511408495567732 3.4312124818360187E-4 52-53 0.0 0.0 0.0 0.9859875000929287 3.4312124818360187E-4 54-55 0.0 0.0 0.0 1.1355025839889332 4.0030812288086883E-4 56-57 0.0 0.0 0.0 1.308635847134909 4.5749499757813584E-4 58-59 0.0 0.0 0.0 1.500869526429772 4.5749499757813584E-4 60-61 0.0 0.0 0.0 1.7193519812106803 4.5749499757813584E-4 62-63 0.0 0.0 0.0 1.9305431094676875 4.5749499757813584E-4 64-65 0.0 0.0 0.0 2.1347860324489765 4.5749499757813584E-4 66-67 0.0 0.0 0.0 2.3791455480303982 4.5749499757813584E-4 68-69 0.0 0.0 0.0 2.652927710643564 4.5749499757813584E-4 70-71 0.0 0.0 0.0 2.9531588028042153 5.146818722754029E-4 72-73 0.0 0.0 0.0 3.2647414895922746 6.004621843213033E-4 74-75 0.0 0.0 0.0 3.5652871095637613 6.290556216699368E-4 76-77 0.0 0.0 0.0 3.8851332997455756 6.290556216699368E-4 78-79 0.0 0.0 0.0 4.260479351821088 6.290556216699368E-4 80-81 0.0 0.0 0.0 4.663303697188637 6.290556216699368E-4 82-83 0.0 0.0 0.0 5.07985289248353 6.290556216699368E-4 84-85 0.0 0.0 0.0 5.494886635598943 6.290556216699368E-4 86-87 0.0 0.0 0.0 5.90780446435056 6.290556216699368E-4 88-89 0.0 0.0 0.0 6.364556032557632 6.290556216699368E-4 90-91 0.0 0.0 0.0 6.871860797997087 6.290556216699368E-4 92-93 0.0 0.0 0.0 7.3819963137340565 6.290556216699368E-4 94-95 0.0 0.0 0.0 7.902911555351462 7.434293710644708E-4 96-97 0.0 0.0 0.0 8.429545484438593 7.434293710644708E-4 98-99 0.0 0.0 0.0 8.967130700030252 7.434293710644708E-4 100-101 0.0 0.0 0.0 9.577486213674181 7.434293710644708E-4 102-103 0.0 0.0 0.0 10.203482337547815 8.292096831103712E-4 104-105 0.0 0.0 0.0 10.83342435577556 8.578031204590047E-4 106-107 0.0 0.0 0.0 11.454759749361365 8.578031204590047E-4 108-109 0.0 0.0 0.0 12.09954176157305 8.578031204590047E-4 110-111 0.0 0.0 0.0 12.78312506826683 8.578031204590047E-4 112-113 0.0 0.0 0.0 13.50442311882346 8.578031204590047E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2850 0.0 73.97164 1 GTATCAA 4885 0.0 52.055916 1 ATCAACG 6110 0.0 40.824238 3 TCAACGC 6185 0.0 40.329197 4 CAACGCA 6250 0.0 40.005024 5 TATCAAC 6275 0.0 39.845642 2 AACGCAG 6510 0.0 38.57362 6 GTACATG 6235 0.0 33.143612 1 ACGCAGA 7735 0.0 32.38311 7 CGCAGAG 7765 0.0 32.258003 8 TACATGG 6495 0.0 30.705128 2 ACATGGG 6270 0.0 30.477737 3 GCAGAGT 9065 0.0 27.566288 9 TAAGGTG 1120 0.0 27.107996 5 GAGTACT 6000 0.0 26.129206 12-13 CAGAGTA 8700 0.0 25.884718 10-11 TGGTATC 1265 0.0 25.412563 2 GTACTTT 6190 0.0 25.086884 14-15 CATGGGA 3235 0.0 25.027063 4 GTAAGGT 1215 0.0 24.988441 4 >>END_MODULE