FastQCFastQC Report
Thu 26 May 2016
SRR1364382_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364382_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2268427
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18600.056.9728931
GTATCAA29800.046.3482061
CAACGCA34850.037.912895
ATCAACG34850.037.742113
TCAACGC36350.036.1846664
GTACATG90000.034.5955621
AACGCAG38400.034.246896
TATCAAC40400.033.5884672
ACATGGG88150.033.3534933
TACATGG94200.032.601342
TAAGGTG15450.029.6618985
GTAAGGT15050.028.8684234
CGCAGAG45900.028.3860768
ACGCAGA46700.027.900427
CATGGGA50350.027.5418534
GGTAAGG15450.026.5801413
CATGGGG46000.026.3942324
AGGTAAG14700.025.1021582
ATGGGAT27850.023.9347825
GTGTAAG9750.023.2027871
Produced by FastQC (version 0.11.5)